JP6316934B2 - 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット - Google Patents
尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット Download PDFInfo
- Publication number
- JP6316934B2 JP6316934B2 JP2016505330A JP2016505330A JP6316934B2 JP 6316934 B2 JP6316934 B2 JP 6316934B2 JP 2016505330 A JP2016505330 A JP 2016505330A JP 2016505330 A JP2016505330 A JP 2016505330A JP 6316934 B2 JP6316934 B2 JP 6316934B2
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- urine sample
- urine
- sample analysis
- iodide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 120
- 210000002700 urine Anatomy 0.000 title claims description 101
- 238000004458 analytical method Methods 0.000 title claims description 23
- 238000012284 sample analysis method Methods 0.000 title claims description 13
- 210000003743 erythrocyte Anatomy 0.000 claims description 50
- 238000005259 measurement Methods 0.000 claims description 43
- -1 EDTA salt Chemical class 0.000 claims description 26
- 125000004432 carbon atom Chemical group C* 0.000 claims description 25
- 239000007850 fluorescent dye Substances 0.000 claims description 24
- 239000002738 chelating agent Substances 0.000 claims description 13
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- XJWSAJYUBXQQDR-UHFFFAOYSA-M dodecyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)C XJWSAJYUBXQQDR-UHFFFAOYSA-M 0.000 claims description 10
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000002270 dispersing agent Substances 0.000 claims description 8
- 230000003287 optical effect Effects 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical group OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- FIZZUEJIOKEFFZ-UHFFFAOYSA-M C3-oxacyanine Chemical compound [I-].O1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2O1 FIZZUEJIOKEFFZ-UHFFFAOYSA-M 0.000 claims description 4
- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 claims description 4
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 claims description 4
- 230000001678 irradiating effect Effects 0.000 claims description 4
- XTYKPEMLMDLYKC-UHFFFAOYSA-N n,n,2-trimethyl-6-propan-2-ylaniline Chemical compound CC(C)C1=CC=CC(C)=C1N(C)C XTYKPEMLMDLYKC-UHFFFAOYSA-N 0.000 claims description 4
- FSVCELGFZIQNCK-UHFFFAOYSA-N N,N-bis(2-hydroxyethyl)glycine Chemical compound OCCN(CCO)CC(O)=O FSVCELGFZIQNCK-UHFFFAOYSA-N 0.000 claims description 3
- 150000001298 alcohols Chemical class 0.000 claims description 3
- QZTKDVCDBIDYMD-UHFFFAOYSA-N 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid Chemical compound NC(=O)CN(CC(O)=O)CC(O)=O QZTKDVCDBIDYMD-UHFFFAOYSA-N 0.000 claims description 2
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 claims description 2
- LVQFQZZGTZFUNF-UHFFFAOYSA-N 2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazine-1,4-diium-1-yl]propane-1-sulfonate Chemical compound OS(=O)(=O)CC(O)CN1CCN(CC(O)CS(O)(=O)=O)CC1 LVQFQZZGTZFUNF-UHFFFAOYSA-N 0.000 claims description 2
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 2
- NUFBIAUZAMHTSP-UHFFFAOYSA-N 3-(n-morpholino)-2-hydroxypropanesulfonic acid Chemical compound OS(=O)(=O)CC(O)CN1CCOCC1 NUFBIAUZAMHTSP-UHFFFAOYSA-N 0.000 claims description 2
- RZQXOGQSPBYUKH-UHFFFAOYSA-N 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCC(CO)(CO)NCC(O)CS(O)(=O)=O RZQXOGQSPBYUKH-UHFFFAOYSA-N 0.000 claims description 2
- XCBLFURAFHFFJF-UHFFFAOYSA-N 3-[bis(2-hydroxyethyl)azaniumyl]-2-hydroxypropane-1-sulfonate Chemical compound OCCN(CCO)CC(O)CS(O)(=O)=O XCBLFURAFHFFJF-UHFFFAOYSA-N 0.000 claims description 2
- 239000007991 ACES buffer Substances 0.000 claims description 2
- 239000007988 ADA buffer Substances 0.000 claims description 2
- 108700016232 Arg(2)-Sar(4)- dermorphin (1-4) Proteins 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- OWXMKDGYPWMGEB-UHFFFAOYSA-N HEPPS Chemical compound OCCN1CCN(CCCS(O)(=O)=O)CC1 OWXMKDGYPWMGEB-UHFFFAOYSA-N 0.000 claims description 2
- GIZQLVPDAOBAFN-UHFFFAOYSA-N HEPPSO Chemical compound OCCN1CCN(CC(O)CS(O)(=O)=O)CC1 GIZQLVPDAOBAFN-UHFFFAOYSA-N 0.000 claims description 2
- 239000007993 MOPS buffer Substances 0.000 claims description 2
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical compound NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims description 2
- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 claims description 2
- 239000007990 PIPES buffer Substances 0.000 claims description 2
- UZMAPBJVXOGOFT-UHFFFAOYSA-N Syringetin Natural products COC1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UZMAPBJVXOGOFT-UHFFFAOYSA-N 0.000 claims description 2
- 239000007997 Tricine buffer Substances 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims description 2
- 208000034953 Twin anemia-polycythemia sequence Diseases 0.000 claims description 2
- 239000007998 bicine buffer Substances 0.000 claims description 2
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 claims description 2
- 230000003139 buffering effect Effects 0.000 claims description 2
- KCFYHBSOLOXZIF-UHFFFAOYSA-N dihydrochrysin Natural products COC1=C(O)C(OC)=CC(C2OC3=CC(O)=CC(O)=C3C(=O)C2)=C1 KCFYHBSOLOXZIF-UHFFFAOYSA-N 0.000 claims description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 2
- 239000004094 surface-active agent Substances 0.000 description 43
- 125000000217 alkyl group Chemical group 0.000 description 25
- 210000003097 mucus Anatomy 0.000 description 16
- 125000003342 alkenyl group Chemical group 0.000 description 15
- 238000010790 dilution Methods 0.000 description 14
- 239000012895 dilution Substances 0.000 description 14
- 239000012192 staining solution Substances 0.000 description 12
- VZUFSMBGWBLOCB-UHFFFAOYSA-N C3-oxacyanine cation Chemical compound O1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2O1 VZUFSMBGWBLOCB-UHFFFAOYSA-N 0.000 description 11
- 239000000975 dye Substances 0.000 description 11
- 150000003839 salts Chemical class 0.000 description 10
- 239000012128 staining reagent Substances 0.000 description 10
- 230000002485 urinary effect Effects 0.000 description 9
- 239000002280 amphoteric surfactant Substances 0.000 description 8
- 230000003204 osmotic effect Effects 0.000 description 8
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 7
- 229910001413 alkali metal ion Inorganic materials 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 125000000304 alkynyl group Chemical group 0.000 description 6
- 239000004065 semiconductor Substances 0.000 description 6
- 239000006172 buffering agent Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000004043 dyeing Methods 0.000 description 5
- 230000005284 excitation Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 4
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 4
- 229960003237 betaine Drugs 0.000 description 4
- 239000003093 cationic surfactant Substances 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 239000002736 nonionic surfactant Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 4
- 206010018910 Haemolysis Diseases 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 150000007942 carboxylates Chemical class 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- XVLXYDXJEKLXHN-UHFFFAOYSA-M dioc6 Chemical compound [I-].O1C2=CC=CC=C2[N+](CCCCCC)=C1C=CC=C1N(CCCCCC)C2=CC=CC=C2O1 XVLXYDXJEKLXHN-UHFFFAOYSA-M 0.000 description 3
- QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000008588 hemolysis Effects 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 3
- 210000005239 tubule Anatomy 0.000 description 3
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 2
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 description 2
- GKLGUKVTTNJEBK-UHFFFAOYSA-N 3-hexyl-2-[3-(3-hexyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole Chemical compound O1C2=CC=CC=C2[N+](CCCCCC)=C1\C=C\C=C1/N(CCCCCC)C2=CC=CC=C2O1 GKLGUKVTTNJEBK-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical class C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 239000003945 anionic surfactant Substances 0.000 description 2
- IOJUPLGTWVMSFF-UHFFFAOYSA-N benzothiazole Chemical compound C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 2
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229960005323 phenoxyethanol Drugs 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 150000003242 quaternary ammonium salts Chemical class 0.000 description 2
- 238000001223 reverse osmosis Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 210000001635 urinary tract Anatomy 0.000 description 2
- 238000011179 visual inspection Methods 0.000 description 2
- FFYRIXSGFSWFAQ-UHFFFAOYSA-N 1-dodecylpyridin-1-ium Chemical compound CCCCCCCCCCCC[N+]1=CC=CC=C1 FFYRIXSGFSWFAQ-UHFFFAOYSA-N 0.000 description 1
- IBLKWZIFZMJLFL-UHFFFAOYSA-N 1-phenoxypropan-2-ol Chemical compound CC(O)COC1=CC=CC=C1 IBLKWZIFZMJLFL-UHFFFAOYSA-N 0.000 description 1
- BKUWWVKLLKXDJK-UHFFFAOYSA-N 2-(dimethylamino)icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCC(N(C)C)C(O)=O BKUWWVKLLKXDJK-UHFFFAOYSA-N 0.000 description 1
- XNCSCQSQSGDGES-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]propyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)C(C)CN(CC(O)=O)CC(O)=O XNCSCQSQSGDGES-UHFFFAOYSA-N 0.000 description 1
- YGDVXSDNEFDTGV-UHFFFAOYSA-N 2-[6-[bis(carboxymethyl)amino]hexyl-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CCCCCCN(CC(O)=O)CC(O)=O YGDVXSDNEFDTGV-UHFFFAOYSA-N 0.000 description 1
- WYMDDFRYORANCC-UHFFFAOYSA-N 2-[[3-[bis(carboxymethyl)amino]-2-hydroxypropyl]-(carboxymethyl)amino]acetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)CN(CC(O)=O)CC(O)=O WYMDDFRYORANCC-UHFFFAOYSA-N 0.000 description 1
- AEDQNOLIADXSBB-UHFFFAOYSA-N 3-(dodecylazaniumyl)propanoate Chemical compound CCCCCCCCCCCCNCCC(O)=O AEDQNOLIADXSBB-UHFFFAOYSA-N 0.000 description 1
- HKJKXDULJBGDED-UHFFFAOYSA-N 3-(tetradecylamino)propanoic acid Chemical compound CCCCCCCCCCCCCCNCCC(O)=O HKJKXDULJBGDED-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- TYPAESUSZUXKRI-UHFFFAOYSA-N C(C(=O)[O-])(=O)[O-].C(CC)(=O)[O-].[NH4+].[NH4+].[NH4+] Chemical compound C(C(=O)[O-])(=O)[O-].C(CC)(=O)[O-].[NH4+].[NH4+].[NH4+] TYPAESUSZUXKRI-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- CXRFDZFCGOPDTD-UHFFFAOYSA-M Cetrimide Chemical compound [Br-].CCCCCCCCCCCCCC[N+](C)(C)C CXRFDZFCGOPDTD-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- FCKYPQBAHLOOJQ-UHFFFAOYSA-N Cyclohexane-1,2-diaminetetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)C1CCCCC1N(CC(O)=O)CC(O)=O FCKYPQBAHLOOJQ-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000006173 Good's buffer Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- BCKXLBQYZLBQEK-KVVVOXFISA-M Sodium oleate Chemical compound [Na+].CCCCCCCC\C=C/CCCCCCCC([O-])=O BCKXLBQYZLBQEK-KVVVOXFISA-M 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 102000018614 Uromodulin Human genes 0.000 description 1
- 108010027007 Uromodulin Proteins 0.000 description 1
- HMNDRWDQGZZYIC-UHFFFAOYSA-N [2-(phosphonomethylamino)ethylamino]methylphosphonic acid Chemical compound OP(O)(=O)CNCCNCP(O)(O)=O HMNDRWDQGZZYIC-UHFFFAOYSA-N 0.000 description 1
- MMBOWXMBUHEDJZ-UHFFFAOYSA-L [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCc1ccccc1 Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCc1ccccc1 MMBOWXMBUHEDJZ-UHFFFAOYSA-L 0.000 description 1
- PYPCLUOPAQYZSY-UHFFFAOYSA-N [Na].C(CCCCCCCCCCCCCCC)(=O)OC Chemical compound [Na].C(CCCCCCCCCCCCCCC)(=O)OC PYPCLUOPAQYZSY-UHFFFAOYSA-N 0.000 description 1
- IPBVNPXQWQGGJP-UHFFFAOYSA-N acetic acid phenyl ester Natural products CC(=O)OC1=CC=CC=C1 IPBVNPXQWQGGJP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000003973 alkyl amines Chemical class 0.000 description 1
- 150000005215 alkyl ethers Chemical class 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- PLMFYJJFUUUCRZ-UHFFFAOYSA-M decyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)C PLMFYJJFUUUCRZ-UHFFFAOYSA-M 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- JWTLFOISPSJINB-UHFFFAOYSA-L disodium tridecylbenzene sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCCCc1ccccc1 JWTLFOISPSJINB-UHFFFAOYSA-L 0.000 description 1
- OZNFKKCYEUFEDW-UHFFFAOYSA-L disodium;decylbenzene;sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCC1=CC=CC=C1 OZNFKKCYEUFEDW-UHFFFAOYSA-L 0.000 description 1
- VVSMKOFFCAJOSC-UHFFFAOYSA-L disodium;dodecylbenzene;sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1 VVSMKOFFCAJOSC-UHFFFAOYSA-L 0.000 description 1
- NSDSAFXDJVYQAG-UHFFFAOYSA-L disodium;tetradecylbenzene;sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O.CCCCCCCCCCCCCCC1=CC=CC=C1 NSDSAFXDJVYQAG-UHFFFAOYSA-L 0.000 description 1
- BHCBQALBZYVPNR-UHFFFAOYSA-N dodecyl 2-amino-2-methylpropanoate Chemical compound CCCCCCCCCCCCOC(=O)C(C)(C)N BHCBQALBZYVPNR-UHFFFAOYSA-N 0.000 description 1
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 1
- AWZOLILCOUMRDG-UHFFFAOYSA-N edifenphos Chemical compound C=1C=CC=CC=1SP(=O)(OCC)SC1=CC=CC=C1 AWZOLILCOUMRDG-UHFFFAOYSA-N 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- IFQUWYZCAGRUJN-UHFFFAOYSA-N ethylenediaminediacetic acid Chemical compound OC(=O)CNCCNCC(O)=O IFQUWYZCAGRUJN-UHFFFAOYSA-N 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- DJQJFMSHHYAZJD-UHFFFAOYSA-N lidofenin Chemical compound CC1=CC=CC(C)=C1NC(=O)CN(CC(O)=O)CC(O)=O DJQJFMSHHYAZJD-UHFFFAOYSA-N 0.000 description 1
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 description 1
- 239000004137 magnesium phosphate Substances 0.000 description 1
- 229960002261 magnesium phosphate Drugs 0.000 description 1
- 229910000157 magnesium phosphate Inorganic materials 0.000 description 1
- 235000010994 magnesium phosphates Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- XCOHAFVJQZPUKF-UHFFFAOYSA-M octyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCC[N+](C)(C)C XCOHAFVJQZPUKF-UHFFFAOYSA-M 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 229940049953 phenylacetate Drugs 0.000 description 1
- WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- BWILYWWHXDGKQA-UHFFFAOYSA-M potassium propanoate Chemical compound [K+].CCC([O-])=O BWILYWWHXDGKQA-UHFFFAOYSA-M 0.000 description 1
- 235000010332 potassium propionate Nutrition 0.000 description 1
- 239000004331 potassium propionate Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- BTURAGWYSMTVOW-UHFFFAOYSA-M sodium dodecanoate Chemical compound [Na+].CCCCCCCCCCCC([O-])=O BTURAGWYSMTVOW-UHFFFAOYSA-M 0.000 description 1
- 229940082004 sodium laurate Drugs 0.000 description 1
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 description 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 1
- 235000010334 sodium propionate Nutrition 0.000 description 1
- 239000004324 sodium propionate Substances 0.000 description 1
- 229960003212 sodium propionate Drugs 0.000 description 1
- 229960000776 sodium tetradecyl sulfate Drugs 0.000 description 1
- MVHWMCQWFWUXEE-GEEYTBSJSA-M sodium;(e)-hexadec-1-ene-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCCC\C=C\S([O-])(=O)=O MVHWMCQWFWUXEE-GEEYTBSJSA-M 0.000 description 1
- NPNCUQAIXIQSKQ-UHFFFAOYSA-M sodium;1-methoxy-1-oxotetradecane-2-sulfonate Chemical compound [Na+].CCCCCCCCCCCCC(S([O-])(=O)=O)C(=O)OC NPNCUQAIXIQSKQ-UHFFFAOYSA-M 0.000 description 1
- WYMNMGKBGAKXHR-UHFFFAOYSA-M sodium;3-hydroxyoctadecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCCCCC(O)CCS([O-])(=O)=O WYMNMGKBGAKXHR-UHFFFAOYSA-M 0.000 description 1
- XZTJQQLJJCXOLP-UHFFFAOYSA-M sodium;decyl sulfate Chemical compound [Na+].CCCCCCCCCCOS([O-])(=O)=O XZTJQQLJJCXOLP-UHFFFAOYSA-M 0.000 description 1
- SZGKXOCIHKEMHV-UHFFFAOYSA-M sodium;octadec-1-ene-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCCCCCC=CS([O-])(=O)=O SZGKXOCIHKEMHV-UHFFFAOYSA-M 0.000 description 1
- RUTSRVMUIGMTHJ-UHFFFAOYSA-M sodium;tetradec-1-ene-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCC=CS([O-])(=O)=O RUTSRVMUIGMTHJ-UHFFFAOYSA-M 0.000 description 1
- UPUIQOIQVMNQAP-UHFFFAOYSA-M sodium;tetradecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCOS([O-])(=O)=O UPUIQOIQVMNQAP-UHFFFAOYSA-M 0.000 description 1
- HQCFDOOSGDZRII-UHFFFAOYSA-M sodium;tridecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCOS([O-])(=O)=O HQCFDOOSGDZRII-UHFFFAOYSA-M 0.000 description 1
- UOWRKHDWHDWJHK-UHFFFAOYSA-M sodium;undecyl sulfate Chemical compound [Na+].CCCCCCCCCCCOS([O-])(=O)=O UOWRKHDWHDWJHK-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 150000003871 sulfonates Chemical class 0.000 description 1
- AQZSPJRLCJSOED-UHFFFAOYSA-M trimethyl(octyl)azanium;chloride Chemical compound [Cl-].CCCCCCCC[N+](C)(C)C AQZSPJRLCJSOED-UHFFFAOYSA-M 0.000 description 1
- CEYYIKYYFSTQRU-UHFFFAOYSA-M trimethyl(tetradecyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)C CEYYIKYYFSTQRU-UHFFFAOYSA-M 0.000 description 1
- 210000004926 tubular epithelial cell Anatomy 0.000 description 1
- 210000003708 urethra Anatomy 0.000 description 1
- 239000004711 α-olefin Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/493—Physical analysis of biological material of liquid biological material urine
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
本実施形態の尿試料分析方法(以下、単に「方法」ともいう)は、尿中有形成分のうち赤血球、円柱、結晶成分及び粘液糸を分析対象とし、特に円柱及び赤血球の分析に好適である。
本実施形態の尿試料分析用試薬11(以下、単に「試薬」ともいう)は、尿試料中の有形成分として少なくとも円柱及び赤血球を検出するための試薬である。本実施形態の試薬は、DiOC2(3)、DiOC3(3)、DiOC4(3)及びDiOC5(3)から選択される少なくとも1つの蛍光色素を含む。なお、本実施形態の試薬については、本実施形態の尿試料分析方法に用いた第1試薬について述べたことと同じである。
本実施形態の尿試料分析用試薬キット(以下、単に「試薬キット」ともいう)は、尿試料中の有形成分として少なくとも円柱及び赤血球を検出するための試薬キットである。この試薬キットは、DiOC2(3)、DiOC3(3)、DiOC4(3)及びDiOC5(3)から選択される少なくとも1つの蛍光色素を含む第1試薬と、分散剤としての界面活性剤を含む第2試薬とを含む。
実施例1では、円柱を染色可能であって、且つ円柱と粘液糸とを染色の差により判別可能な色素を探索した。なお、円柱と粘液糸との判別は、試料を蛍光顕微鏡下で観察することにより行った。また、フローサイトメータによる測定も行った。
尿試料として、円柱及び粘液糸を含む尿を用いた。
(2)試薬
・染色用試薬
染色用試薬として、表1に示される各色素を含む染色液1〜29を調製した。なお、これらの染色液はいずれも、色素を1mg/mLの濃度となるようにエチレングリコール(ナカライテスク株式会社)に溶解して調製した。
希釈用試薬として、HEPES-OH(100 mM、pH7)(株式会社同仁化学研究所)を用いた。なお、溶媒には、逆浸透膜で濾過した水を用いた。
尿試料(200μL)と、希釈用試薬(580μL)と、各染色液(20μL)とを混合し、40℃にて1分間反応させ測定試料を調製した。そして、得られた測定試料中の円柱及び粘液糸を蛍光顕微鏡BX51(オリンパス株式会社製)により観察した。観察の結果、上記の29種の色素のうち、円柱を染色可能であって、且つ円柱と粘液糸とを染色の差により判別可能な色素は、DioC2(3)、DioC3(3)、DioC4(3)、DioC5(3)、DioC6(3)及びDioC7(3)の6種のみであった。これらの色素を用いた場合、粘液糸よりも円柱が良好に染色され、その結果、円柱と粘液糸との判別が可能であった。
蛍光顕微鏡による観察結果を受けて、染色液1〜6のそれぞれで染色した試料について、フローサイトメータによっても円柱と粘液糸とを弁別可能であるかを検討した。試料の測定は、フローサイトメータUF-1000i(シスメックス株式会社製)を用いて行った。このフローサイトメータによる測定の具体的な工程は、次のとおりである。まず、尿試料(200μL)と、希釈用試薬(580μL)と、各染色液(20μL)とを混合し、40℃にて60秒間反応させて測定試料を調製した。そして、測定試料に光を照射して、前方散乱光強度、側方散乱光強度、蛍光強度及び蛍光の積分値を取得した。なお、フローサイトメータの光源として、励起波長488 nmの半導体レーザを用いた。蛍光強度及び蛍光の積分値から、円柱の粘液糸に対する蛍光強度比及び蛍光総和比を算出した。結果を表2に示す。
実施例2では、DioC2(3)、DioC3(3)、DioC4(3)、DioC5(3)、DioC6(3)及びDioC7(3)による赤血球への影響(溶血)を検討した。なお、赤血球への影響は、測定試料中の赤血球数に基づいて評価した。
健常ボランティアから採取したヒト血液を生理食塩水(大塚製薬工場)で1000倍に希釈して、赤血球試料を調製した。
(2)試薬
染色用試薬として、実施例1と同じ染色液1〜6を用いた。希釈用試薬として、実施例1と同じ緩衝液を用いた。
試料の測定はフローサイトメータUF-1000i(シスメックス株式会社製)を用いて行った。なお、測定試料は実施例1と同様にして調製した。そして、測定試料に光を照射して、蛍光強度及び前方散乱光強度を取得した。なお、フローサイトメータの光源として、励起波長488 nmの半導体レーザを用いた。そして、これらの測定値に基づいて、測定試料中の赤血球数をカウントした。結果を表3に示す。
実施例3では、フローサイトメータを用いる本実施形態の尿試料分析方法の臨床性能を、目視検査の結果と比較して評価した。
尿試料として、顕微鏡観察により円柱が出現していないと判断された陰性尿検体(42検体)を用いた。
・染色用試薬
染色用試薬として、実施例1と同じ染色液2(DioC3(3)含有)を用いた。
・希釈用試薬
希釈用試薬として、下記の組成の希釈液1及び2を調製した。なお、希釈液1及び2には、逆浸透膜で濾過した水を溶媒として用いた。
希釈液1:HEPES-OH(100 mM、pH7)及びEDTA-2K(25 mM)(中部キレスト株式会社)
希釈液2:HEPES-OH(100 mM、pH7)、EDTA-2K(25 mM)及びDTAB(10 ppm)(東京化成工業株式会社)
試料の測定はフローサイトメータUF-1000i(シスメックス株式会社製)を用いて行った。このフローサイトメータによる測定の具体的な工程は、次のとおりである。まず、尿試料(200μL)と、希釈用試薬(580μL)と、各染色液(20μL)とを混合し、40℃にて10秒間反応させて測定試料を調製した。そして、得られた測定試料に光を照射して、前方散乱光強度、側方散乱光強度及び蛍光強度を取得した。なお、フローサイトメータの光源として、励起波長488 nmの半導体レーザを用いた。これらの測定値に基づいて測定試料中の円柱の数を算出し、フローサイトメータ(FCM)により陰性と判断された検体数を求めた。また、目視(顕微鏡観察)による陰性検体数をリファレンスとして、FCMによる円柱の分析の特異度を求めた。結果を表4に示す。
22 第1試薬
33 第2試薬
Claims (15)
- 尿試料と、3,3'-ジエチルオキサカルボシアニンアイオダイド、3,3-ジプロピルオキサカルボシアニンアイオダイド、3,3'-ジブチルオキサシアニンアイオダイド及び3,3-ジペンチルオキサカルボシアニンアイオダイドから選択される少なくとも1つの蛍光色素を含む第1試薬と、分散剤としてのドデシルトリメチルアンモニウムブロミドを含む第2試薬とを混合して測定試料を調製する工程と、
調製工程で得られた測定試料に含まれる尿中有形成分として少なくとも円柱及び赤血球を検出する工程と
を含む、尿試料分析方法。 - 第2試薬が、キレート剤をさらに含む請求項1に記載の尿試料分析方法。
- キレート剤が、エチレンジアミン四酢酸塩(EDTA塩)である請求項2に記載の尿試料分析方法。
- 調製工程で得られた測定試料に含まれる尿中有形成分に光を照射して光学的情報を取得する工程をさらに含み、
検出工程が、取得した光学的情報に基づいて、尿中有形成分として少なくとも円柱及び赤血球を検出する工程である請求項1〜3のいずれか1項に記載の尿試料分析方法。 - 光学的情報が、散乱光情報および蛍光情報である請求項4に記載の尿試料分析方法。
- 3,3'-ジエチルオキサカルボシアニンアイオダイド、3,3-ジプロピルオキサカルボシアニンアイオダイド、3,3'-ジブチルオキサシアニンアイオダイド及び3,3-ジペンチルオキサカルボシアニンアイオダイドから選択される少なくとも1つの蛍光色素を含む第1試薬と、
分散剤としてのドデシルトリメチルアンモニウムブロミドを含む第2試薬と
を含む、尿中有形成分として少なくとも円柱及び赤血球を検出するための尿試料分析用試薬キット。 - 第2試薬が、キレート剤をさらに含む請求項6に記載の尿試料分析用試薬キット。
- キレート剤が、エチレンジアミン四酢酸塩(EDTA塩)である請求項7に記載の尿試料分析用試薬キット。
- 請求項1〜5のいずれか1項に記載の尿試料分析方法に用いられ、分散剤としてのドデシルトリメチルアンモニウムブロミド、前記ドデシルトリメチルアンモニウムブロミドを溶解するための溶媒、及び緩衝剤を含む、試薬。
- 前記溶媒が、水、水溶性有機溶媒及びそれらの混合物から選択される請求項9に記載の試薬。
- 前記水溶性有機溶媒が、炭素数1〜3の低級アルコール、エチレングリコール及びジメチルスルホキシドから選択される請求項10に記載の試薬。
- 前記緩衝剤が、pH5以上9以下の範囲で緩衝作用を有する緩衝剤である請求項9〜11のいずれか1項に記載の試薬。
- 前記緩衝剤が、Tris、MES、Bis-Tris、ADA、PIPES、ACES、MOPS、MOPSO、BES、TES、HEPES、DIPSO、TAPSO、POPSO、HEPPSO、EPPS、Tricine、Bicine及びTAPSから選択される請求項12に記載の試薬。
- キレート剤をさらに含む請求項9〜13のいずれか1項に記載の試薬。
- 前記キレート剤が、エチレンジアミン四酢酸塩(EDTA塩)である請求項14に記載の試薬。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014039281 | 2014-02-28 | ||
JP2014039281 | 2014-02-28 | ||
PCT/JP2015/055897 WO2015129869A1 (ja) | 2014-02-28 | 2015-02-27 | 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2015129869A1 JPWO2015129869A1 (ja) | 2017-03-30 |
JP6316934B2 true JP6316934B2 (ja) | 2018-04-25 |
Family
ID=54009182
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2016505330A Active JP6316934B2 (ja) | 2014-02-28 | 2015-02-27 | 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット |
Country Status (9)
Country | Link |
---|---|
US (1) | US10502685B2 (ja) |
EP (1) | EP3112862B1 (ja) |
JP (1) | JP6316934B2 (ja) |
KR (2) | KR20160126066A (ja) |
CN (1) | CN106062556B (ja) |
ES (1) | ES2696836T3 (ja) |
HU (1) | HUE041665T2 (ja) |
SG (1) | SG11201607146RA (ja) |
WO (1) | WO2015129869A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11201607145VA (en) * | 2014-02-28 | 2016-10-28 | Sysmex Corp | Method for urine sample analysis, reagent for urine sample analysis, and reagent kit for urine sample analysis |
WO2022091418A1 (ja) * | 2020-11-02 | 2022-05-05 | 株式会社ヘルスケアシステムズ | 尿検体を保存するための試薬、キット及び方法 |
CN115791717B (zh) * | 2022-09-22 | 2024-07-26 | 四川大学华西医院 | 基于竞争选择性识别和二元可视化检测还原性盐的检测方法及其应用 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3070968B2 (ja) * | 1991-05-14 | 2000-07-31 | シスメックス株式会社 | 尿中の細胞分析用試薬及び方法 |
EP0708334B1 (en) * | 1994-10-20 | 2001-05-23 | Sysmex Corporation | Reagent and method for analyzing solid components in urine |
JP3580615B2 (ja) | 1994-10-20 | 2004-10-27 | シスメックス株式会社 | 尿中有形成分分析用試薬及びその試薬を用いた有形成分分析方法 |
JP3467340B2 (ja) | 1995-03-06 | 2003-11-17 | シスメックス株式会社 | 尿中赤血球の鑑別装置 |
JP3739482B2 (ja) | 1996-05-23 | 2006-01-25 | シスメックス株式会社 | 尿中の有形成分分析方法および試薬 |
JP3642658B2 (ja) | 1997-06-30 | 2005-04-27 | シスメックス株式会社 | 尿中有形成分分析装置および分析方法 |
JP2002090365A (ja) * | 2000-02-08 | 2002-03-27 | Internatl Reagents Corp | 腎障害の検査手段 |
US20030224445A1 (en) | 2000-07-13 | 2003-12-04 | Osamu Hotta | Means for examining nephropathy |
US7309581B2 (en) * | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
JP4413120B2 (ja) * | 2004-09-30 | 2010-02-10 | シスメックス株式会社 | 検体中粒子分析方法及び分析装置 |
JP4759438B2 (ja) | 2006-05-17 | 2011-08-31 | シスメックス株式会社 | 尿中有形成分分析装置 |
CN101246158B (zh) * | 2008-03-21 | 2012-04-18 | 王亚东 | 测定血细胞中白细胞的溶血剂 |
CN102226804B (zh) * | 2011-03-28 | 2013-07-03 | 中国人民解放军总医院 | 一种用于血液白细胞五分类计数的溶血剂及其用途 |
CN105026928B (zh) * | 2013-02-28 | 2018-11-06 | 希森美康株式会社 | 尿样本分析装置及尿样本分析方法 |
JP6189686B2 (ja) | 2013-09-10 | 2017-08-30 | 独立行政法人国立高等専門学校機構 | 光線による避難誘導システム |
-
2015
- 2015-02-27 US US15/121,250 patent/US10502685B2/en active Active
- 2015-02-27 JP JP2016505330A patent/JP6316934B2/ja active Active
- 2015-02-27 HU HUE15754422A patent/HUE041665T2/hu unknown
- 2015-02-27 WO PCT/JP2015/055897 patent/WO2015129869A1/ja active Application Filing
- 2015-02-27 SG SG11201607146RA patent/SG11201607146RA/en unknown
- 2015-02-27 ES ES15754422T patent/ES2696836T3/es active Active
- 2015-02-27 CN CN201580010653.0A patent/CN106062556B/zh active Active
- 2015-02-27 KR KR1020167026680A patent/KR20160126066A/ko active Search and Examination
- 2015-02-27 KR KR1020187021877A patent/KR102024497B1/ko active IP Right Grant
- 2015-02-27 EP EP15754422.2A patent/EP3112862B1/en active Active
Also Published As
Publication number | Publication date |
---|---|
US10502685B2 (en) | 2019-12-10 |
SG11201607146RA (en) | 2016-10-28 |
HUE041665T2 (hu) | 2019-05-28 |
EP3112862A1 (en) | 2017-01-04 |
CN106062556A (zh) | 2016-10-26 |
JPWO2015129869A1 (ja) | 2017-03-30 |
EP3112862B1 (en) | 2018-10-24 |
WO2015129869A1 (ja) | 2015-09-03 |
EP3112862A4 (en) | 2017-09-27 |
KR102024497B1 (ko) | 2019-09-23 |
KR20180088529A (ko) | 2018-08-03 |
ES2696836T3 (es) | 2019-01-18 |
CN106062556B (zh) | 2019-07-12 |
US20160363535A1 (en) | 2016-12-15 |
KR20160126066A (ko) | 2016-11-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1755348B (zh) | 检体中粒子分析方法、分析装置及分析试剂 | |
JP3886271B2 (ja) | 赤芽球の分類計数用試薬及び分類計数方法 | |
RU2461004C2 (ru) | Набор реагентов для анализа образца и способ анализа образца | |
WO2021240914A1 (ja) | 尿沈渣用染色液 | |
JP4794334B2 (ja) | 尿試料分析用試薬及び尿試料の分析方法 | |
JP6316934B2 (ja) | 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット | |
JP6316933B2 (ja) | 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット | |
JP3580615B2 (ja) | 尿中有形成分分析用試薬及びその試薬を用いた有形成分分析方法 | |
JP6316935B2 (ja) | 尿試料分析方法及び尿試料分析用試薬キット | |
JPH1183849A (ja) | 尿中有形成分の分析方法及びその分析試薬 | |
JP3888876B2 (ja) | 細菌の染色方法、細菌の分類・計数方法及び細菌染色用希釈液 | |
JP2010002428A (ja) | 尿中赤血球分析用試薬 | |
Al-Satar et al. | EVALUATION OF PERMANENT STAIN USED FOR FIXED SMEARS OF SOME INTESTINAL PARASITES IN STAINING OF URINE SEDIMENT SMEAR |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20161130 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170112 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170523 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170721 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20171010 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20171201 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20180306 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20180328 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6316934 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |