JP6300727B2 - コバインダー補助アッセイ法 - Google Patents

コバインダー補助アッセイ法 Download PDF

Info

Publication number
JP6300727B2
JP6300727B2 JP2014541281A JP2014541281A JP6300727B2 JP 6300727 B2 JP6300727 B2 JP 6300727B2 JP 2014541281 A JP2014541281 A JP 2014541281A JP 2014541281 A JP2014541281 A JP 2014541281A JP 6300727 B2 JP6300727 B2 JP 6300727B2
Authority
JP
Japan
Prior art keywords
binding
oligonucleotide sequence
analyte
oligonucleotide
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014541281A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014533364A5 (https=
JP2014533364A (ja
Inventor
イーライ・エヌ・グレザー
ジョージ・シガル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JP2014533364A publication Critical patent/JP2014533364A/ja
Publication of JP2014533364A5 publication Critical patent/JP2014533364A5/ja
Application granted granted Critical
Publication of JP6300727B2 publication Critical patent/JP6300727B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2014541281A 2011-11-11 2012-11-09 コバインダー補助アッセイ法 Active JP6300727B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161558537P 2011-11-11 2011-11-11
US61/558,537 2011-11-11
PCT/US2012/064263 WO2013070990A1 (en) 2011-11-11 2012-11-09 Co-binder assisted assay methods

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017231321A Division JP6795733B2 (ja) 2011-11-11 2017-12-01 コバインダー補助アッセイ法

Publications (3)

Publication Number Publication Date
JP2014533364A JP2014533364A (ja) 2014-12-11
JP2014533364A5 JP2014533364A5 (https=) 2015-12-17
JP6300727B2 true JP6300727B2 (ja) 2018-03-28

Family

ID=48290570

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2014541281A Active JP6300727B2 (ja) 2011-11-11 2012-11-09 コバインダー補助アッセイ法
JP2017231321A Active JP6795733B2 (ja) 2011-11-11 2017-12-01 コバインダー補助アッセイ法
JP2020079957A Pending JP2020126068A (ja) 2011-11-11 2020-04-30 コバインダー補助アッセイ法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2017231321A Active JP6795733B2 (ja) 2011-11-11 2017-12-01 コバインダー補助アッセイ法
JP2020079957A Pending JP2020126068A (ja) 2011-11-11 2020-04-30 コバインダー補助アッセイ法

Country Status (6)

Country Link
US (4) US9777338B2 (https=)
EP (2) EP3467500B1 (https=)
JP (3) JP6300727B2 (https=)
DK (2) DK3467500T3 (https=)
ES (2) ES2884100T3 (https=)
WO (1) WO2013070990A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3467500B1 (en) 2011-11-11 2021-05-12 Eli N. Glezer Co-binder assisted assay methods
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904181A1 (en) 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
WO2015175856A1 (en) 2014-05-15 2015-11-19 Meso Scale Technologies, Llc. Improved assay methods
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
EP3356547B1 (en) * 2015-10-01 2020-11-18 Dynamic Biosensors GmbH Method for detecting molecular interactions using an immobilized nucleic acid
EP3448886A4 (en) * 2016-04-26 2019-12-11 Ultivue, Inc. SUPER-HIGH-RESOLUTION IMMUNOFLUORESCENCE WITH BINDING-LIMITED PREVIEW
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11796535B2 (en) 2016-09-16 2023-10-24 Plexbio Co., Ltd. Methods and systems for multiplex assays
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
AU2020232954B2 (en) 2019-03-01 2026-01-15 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11619589B2 (en) 2020-07-20 2023-04-04 United Arab Emirates University Method and system for detection, quantification and/or identification of an analyte in a specimen based on electrical and/or optical response to an electric field
WO2022018622A1 (en) 2020-07-20 2022-01-27 United Arab Emirates University Method and system for virus and protein-antibody interactions detection and monitoring based on optical light intensity and electrical parameters
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
WO2023080137A1 (ja) * 2021-11-04 2023-05-11 凸版印刷株式会社 標的物質の検出方法

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310687A (en) 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US5238808A (en) 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
US5591581A (en) 1986-04-30 1997-01-07 Igen, Inc. Electrochemiluminescent rhenium moieties and methods for their use
US6165729A (en) 1986-04-30 2000-12-26 Hyperion Catalysis International, Inc. Electrochemiluminescent reaction utilizing amine-derived reductant
US5147806A (en) 1988-04-29 1992-09-15 Igen, Inc. Method and apparatus for conducting electrochemiluminescence measurements
US5308754A (en) 1988-03-21 1994-05-03 Kankare Jouko J Electrogenerated luminescence in solution
US5093268A (en) 1988-04-28 1992-03-03 Igen, Inc. Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena
US5705402A (en) 1988-11-03 1998-01-06 Igen International, Inc. Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets
US5324457A (en) 1989-10-02 1994-06-28 Board Of Regents, The University Of Tx System Devices and methods for generating electrogenerated chemiluminescence
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5776672A (en) 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
JPH0534345A (ja) 1991-02-19 1993-02-09 Tdk Corp 化学発光を利用する抗原・抗体の測定方法
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
ZA929351B (en) 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
DE69432419T2 (de) * 1993-09-22 2004-01-08 Igen International, Inc. Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
US5786141A (en) 1994-08-26 1998-07-28 Bard; Allen J. Electrogenerated chemiluminescence labels for analysis and/or referencing
US5866434A (en) 1994-12-08 1999-02-02 Meso Scale Technology Graphitic nanotubes in luminescence assays
US5641623A (en) 1995-01-04 1997-06-24 Martin; Mark T. Electrochemiluminescence assay
US5643713A (en) 1995-06-07 1997-07-01 Liang; Pam Electrochemiluminescent monitoring of compounds
EP0821726B1 (en) 1995-03-10 2014-05-07 Meso Scale Technologies, LLC. Multi-array, multi-specific electrochemiluminescence testing
US6207369B1 (en) 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6673533B1 (en) * 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
US5679519A (en) 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
US6319670B1 (en) 1995-05-09 2001-11-20 Meso Scale Technology Llp Methods and apparatus for improved luminescence assays using microparticles
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
GB9606850D0 (en) 1996-04-01 1996-06-05 Univ Liverpool An assay system and novel labelled compounds for use therwith
JP2001523111A (ja) * 1997-05-05 2001-11-20 サード ウェーブ テクノロジーズ，インコーポレーテッド 構造体架橋オリゴヌクレオチドを使用した標的依存型反応
US7314711B2 (en) * 1997-05-23 2008-01-01 Bioveris Corporation Assays employing electrochemiluminescent labels and electrochemiluminescence quenchers
FR2764381A1 (fr) 1997-06-09 1998-12-11 Univ De Neuchatel Detecteur electrochimioluminescent
US6413783B1 (en) 1997-09-18 2002-07-02 Meso Scale Technologies, Llc Assay sonication apparatus and methodology
US6200531B1 (en) 1998-05-11 2001-03-13 Igen International, Inc. Apparatus for carrying out electrochemiluminescence test measurements
US8063190B2 (en) 1998-06-02 2011-11-22 Tom Robin Caine Boyde Nucleic acid-linked conjugates and methods for making and using them
EP0962773A1 (en) 1998-06-03 1999-12-08 Mark Howard Jones Electrochemical based assay processes instrument and labels
GB9815042D0 (en) 1998-07-10 1998-09-09 Imperial College Detector
US6214552B1 (en) 1998-09-17 2001-04-10 Igen International, Inc. Assays for measuring nucleic acid damaging activities
US6136268A (en) 1999-08-17 2000-10-24 Orion Diagnostica Method for luminescence measurements
US6800608B2 (en) * 2001-06-22 2004-10-05 Beckman Coulter, Inc. Homogeneous assay of vancomycin using a stable particle-vancomycin conjugate, a novel rate enhancer, and a novel dose response modulator
AU2003291249A1 (en) 2002-12-18 2004-07-29 Aclara Biosciences, Inc. Multiplexed immunohistochemical assays using molecular tags
AU2004232976B2 (en) * 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
US7195875B2 (en) * 2003-04-18 2007-03-27 Beckman Coulter, Inc. Oligonucleotide pairs for multiplexed binding assays
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
CN101208437B (zh) 2003-12-12 2012-07-04 圣路易斯大学 检测大分子和其它分析物的生物传感器
EP2511383B1 (en) 2006-03-31 2013-12-25 Columbia University Binary probes for fluorescent analysis of nucleic acids
EP2010475A2 (en) * 2006-04-27 2009-01-07 Intezyne Technologies Incorporated Heterofunctional poly(ethylene glycol) containing acid-labile amino protecting groups and uses thereof
JP5329038B2 (ja) * 2006-12-21 2013-10-30 宇部日東化成株式会社 半導体装置及び半導体装置の製造方法
US20120003632A1 (en) 2007-11-21 2012-01-05 Life Science Patents fret-probes and use thereof
JP5435878B2 (ja) * 2008-02-12 2014-03-05 富士フイルム株式会社 断片化抗体を標識物質に固定した標識粒子
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET
US20100261292A1 (en) * 2009-04-10 2010-10-14 Meso Scale Technologies, Llc Methods for Conducting Assays
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
WO2011143583A1 (en) * 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
EP3467500B1 (en) 2011-11-11 2021-05-12 Eli N. Glezer Co-binder assisted assay methods

Also Published As

Publication number Publication date
US20140315189A1 (en) 2014-10-23
JP2020126068A (ja) 2020-08-20
ES2700792T3 (es) 2019-02-19
EP2776833A4 (en) 2015-12-02
JP2018054629A (ja) 2018-04-05
US20170362668A1 (en) 2017-12-21
DK2776833T3 (da) 2019-01-02
EP3467500A1 (en) 2019-04-10
EP2776833B1 (en) 2018-09-05
EP3467500B1 (en) 2021-05-12
JP2014533364A (ja) 2014-12-11
US11242570B2 (en) 2022-02-08
DK3467500T3 (da) 2021-08-16
US20250327141A1 (en) 2025-10-23
US20220033918A1 (en) 2022-02-03
US12359264B2 (en) 2025-07-15
ES2884100T3 (es) 2021-12-10
EP2776833A1 (en) 2014-09-17
JP6795733B2 (ja) 2020-12-02
WO2013070990A1 (en) 2013-05-16
US9777338B2 (en) 2017-10-03

Similar Documents

Publication Publication Date Title
JP6300727B2 (ja) コバインダー補助アッセイ法
AU2020200675B2 (en) Improved methods for conducting multiplexed assays
US20200191779A1 (en) Methods for conducting assays
EP3644058A1 (en) Reagent storage in an assay device
HK1213048B (en) Improved methods for conducting multiplexed assays

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151023

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151023

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160816

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20160817

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161116

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170215

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20170801

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171201

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20171206

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20171227

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20180130

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180227

R150 Certificate of patent or registration of utility model

Ref document number: 6300727

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250