JP6165555B2 - Automatic analyzer and its dispensing performance confirmation method - Google Patents

Description

  Embodiments described herein relate generally to an automatic analyzer that dispenses a sample collected from a subject and analyzes components contained in the sample, and a method for confirming the dispensing performance thereof.

  Tests using automatic analyzers target biochemical test items, immunological test items, etc., and optically detect changes in color and turbidity caused by the reaction of the mixture of the sample collected from the specimen and the reagent used to analyze each test item. Measure automatically. By this measurement, analytical data represented by the concentrations of various test item components in the sample, enzyme activities, and the like are generated.

  The automatic analyzer includes analysis units such as a sample dispensing probe, a reagent dispensing probe, a measuring unit, and a plurality of reaction containers. The sample dispensing probe dispenses the sample stored in the sample container into the reaction container. The reagent dispensing probe dispenses a reagent contained in the reagent container into the reaction container. The measurement unit measures a mixed solution of the sample and the reagent in the reaction container.

  In recent years, in automatic analyzers, the amount of samples and reagents has been reduced in consideration of the burden on the subject and the reduction of running costs. In particular, since the amount of sample to be dispensed into the reaction container is extremely small compared to the reagent, a technique for accurately dispensing a small amount of sample into the reaction container is required. Then, in order to confirm whether or not a small amount of sample is accurately dispensed into the reaction vessel, a confirmation sample such as a control sample for accuracy control is dispensed into the reaction vessel, and measurement of the mixed liquid containing the sample is performed. The dispensing performance is confirmed based on the data obtained by the above.

JP 2009-281914 A

  However, in order to perform confirmation, preparation and operation of a sample and the like different from those in the case of performing inspection are necessary, and there is a problem that the confirmation work becomes complicated and troublesome.

  Embodiments have been made to solve the above-described problems, and an object thereof is to provide an automatic analyzer and a method for confirming its dispensing performance that can reduce the confirmation work and perform performance confirmation quickly. .

In order to achieve the above object, an automatic analyzer according to an embodiment includes a plurality of reaction containers, a sample dispensing probe for dispensing a sample contained in a sample container into the reaction container, and a reagent contained in a reagent container. For checking the reagent contained in the reagent container in an automatic analyzer equipped with a reagent dispensing probe to be dispensed into a reaction container and a measuring unit for measuring a mixed solution of the sample and reagent dispensed in the reaction container As a reagent, the reagent dispensing probe dispenses the reaction container, the confirmation reagent dispensed in the reaction container is dispensed into the reaction container other than the reaction container by the sample dispensing probe, and the sample dispensing A diluent is discharged from the reagent dispensing probe or the sample dispensing probe into the reaction container into which the confirmation reagent has been dispensed by the probe, and the confirmation reagent in the reaction container is Dilution liquid is seeking diluted value indicating the variation of a plurality of confirmation data generated by measuring the diluted reagent by the measurement unit was, minutes of the sample if the value is within the allowable range set in advance A performance confirmation unit is provided that determines that the injection is performed normally and determines that the dispensing performance of the sample by the sample dispensing probe is degraded when the value is out of the allowable range . It is characterized by that.

The block diagram which shows the structure of the automatic analyzer which concerns on embodiment. The perspective view which shows the structure of the analysis part which concerns on embodiment. The figure which shows an example of the performance confirmation parameter setting screen displayed on the display part which concerns on embodiment. The figure which shows an example of a structure of the sample dispensing unit regarding the dispensing of the sample dispensing probe which concerns on embodiment. The figure which shows an example of each stop position of each reaction container hold | maintained at the reaction table which concerns on embodiment, and the position of a measurement part. The figure which shows the washing position of the washing nozzle which concerns on embodiment, and each dispensing position of a sample dispensing probe, a 1st reagent dispensing probe, and a 2nd reagent dispensing probe. The figure which shows an example of operation | movement of the washing nozzle in each performance confirmation which concerns on embodiment, each reaction container, a 1st reagent dispensing probe, and a sample dispensing probe. The figure which shows an example of operation | movement of the washing nozzle in each performance confirmation which concerns on embodiment, each reaction container, a 1st reagent dispensing probe, and a sample dispensing probe.

  Hereinafter, embodiments will be described with reference to the drawings.

  FIG. 1 is a block diagram illustrating a configuration of an automatic analyzer according to the embodiment. This automatic analyzer 100 is configured to supply each liquid such as a standard sample, a test sample, a reagent for analyzing each inspection item, a reagent for confirming performance selected from these reagents (a reagent for confirmation), and a diluent. The analysis unit 10 is provided for dispensing and measuring a mixed solution of a sample and a reagent, a confirmation reagent, and a diluted reagent obtained by diluting the confirmation reagent with a diluent. Moreover, the drive part 40 which drives each analysis unit of the analysis part 10 and the analysis control part 41 which controls the analysis part 10 and the drive part 40 are provided.

  The automatic analyzer 100 also includes a performance confirmation unit 50 that confirms the dispensing performance of the analysis unit 10 based on reference data and confirmation data generated by the measurement of the confirmation reagent and the diluted reagent in the analysis unit 10. ing. Further, a data processing unit 60 that processes standard data and test data generated by measurement of a mixed solution containing a standard sample and a mixed solution containing a test sample in the analysis unit 10 to generate calibration data and analysis data is provided. I have.

  Furthermore, the automatic analyzer 100 includes an output unit 70 that prints out and displays the calibration data and analysis data generated by the data processing unit 60, an analysis parameter for each inspection item, and an analysis range in which the analysis data can be analyzed. And an operation unit 80 for inputting a re-inspection parameter for performing re-inspection when it is off, input of a confirmation parameter for confirming dispensing performance, input of start of inspection or performance confirmation, and the like. ing. In addition, a system control unit 90 that controls the analysis control unit 41, the performance confirmation unit 50, the data processing unit 60, and the output unit 70 is provided.

  FIG. 2 is a perspective view showing the configuration of the analysis unit 10. The analysis unit 10 includes a sample container 11 for storing a sample such as a standard sample or a test sample, and a sample table 12 for holding the sample container 11 so as to be movable. In addition, for example, a 1-reagent system and 2-reagent system first reagent, a 2-reagent system second reagent, a test sample and a confirmation reagent are diluted to prepare a diluted sample and a diluted reagent for analysis of each test item. A reagent container 13 for storing a diluent is provided.

  In addition, a reagent store 15 for keeping the first reagent in the reagent container 13 cool, a reagent store 16 for keeping the second reagent in the reagent container 13 cool, and a reagent store 15 for holding the reagent container 13 movably. , 16 and two reagent racks 14 are provided. Moreover, the reaction table 18 which hold | maintains 165 reaction containers 17 arrange | positioned in a line at equal intervals on the circumference so that rotation movement is possible is provided.

  In addition, the sample dispensing probe 19 for dispensing the sample in the sample container 11 held on the sample table 12 and sucking it into the reaction container 17, and the sample dispensing probe 19 can be rotated and moved up and down. And a sample dispensing arm 20 to be held in the chamber. In addition, a first reagent dispensing probe 21 that dispenses the first reagent and diluent in the reagent container 13 held in the reagent rack 14 and sucks them into the reaction container 17 and the first reagent dispensing probe. And a first reagent dispensing arm 22 that holds 21 in a rotatable and vertically movable manner.

  Also, a first stirrer 23 that stirs the mixed solution of the sample and the first reagent dispensed in the reaction container 17, and a first stirrer arm 24 that holds the first stirrer 23 so as to be capable of rotating and moving up and down. It has. In addition, the second reagent dispensing probe 25 for dispensing the second reagent in the reagent container 13 held in the reagent rack 14 and sucking it into the reaction container 17 and the second reagent dispensing probe 25 are rotated. And a second reagent dispensing arm 26 that can be moved and moved up and down.

  In addition, a second stirrer 27 that stirs the sample dispensed in the reaction vessel 17, a mixed solution of the first reagent and the second reagent, and a second stirrer 27 that holds the second stirrer 27 so as to be capable of rotating and vertically moving. And a stirring arm 28. Further, a measuring unit 29 that optically measures the mixed liquid in the reaction vessel 17 and a cleaning nozzle 30 that cleans the inside of the reaction vessel 17 that has been measured by the measuring unit 29 are provided.

  Then, the measurement unit 29 irradiates the reaction container 17 with light, and, for example, changes in absorbance and absorbance are detected based on a detection signal that detects light transmitted through the mixed solution containing the standard sample and the test sample in the reaction container 17. Generate standard data and test data expressed in quantities. Further, based on a detection signal for detecting light transmitted through the confirmation reagent and dilution reagent in the reaction container 17, reference data represented by absorbance and confirmation data are generated. Then, the generated standard data and test data are output to the data processing unit 60. Also, the generated reference data and confirmation data are output to the performance confirmation unit 50.

  The drive unit 40 illustrated in FIG. 1 drives the sample table 12 of the analysis unit 10 to move the sample container 11. Further, each reagent rack 14 is driven to rotate the reagent container 13. Further, the reaction table 18 is driven to rotate the reaction vessel 17. Further, the sample dispensing arm 20, the first reagent dispensing arm 22, the second reagent dispensing arm 26, the first stirring arm 24, and the second stirring arm 28 are respectively rotated and driven up and down to sample dispensing. The probe 19, the first reagent dispensing probe 21, the second reagent dispensing probe 25, the first stirring bar 23, and the second stirring bar 27 are rotated and moved up and down, respectively. Further, the cleaning nozzle 30 is moved up and down.

  The analysis control unit 41 controls the measurement unit 29 and the drive unit 40 of the analysis unit 10. Then, based on the analysis parameters of each inspection item input from the operation unit 80, each analysis unit of the analysis unit 10 performs dispensing of a sample, a reagent, and the like and measurement of the mixed solution. In addition, based on the confirmation parameter input from the operation unit 80, the first or second reagent amount in the reagent container 13 set as the confirmation parameter of the analysis unit 10 is used as the confirmation reagent. Dispensing into the reaction vessel 17 with the injection probes 21 and 25, and the confirmation reagent dispensed into the reaction vessel 17 is dispensed into the reaction vessel 17 other than the reaction vessel 17 with the sample dispensing probe 19, and the sample dispensing probe The dilution liquid is discharged from the first reagent dispensing probe 21 to the reaction container 17 into which the confirmation reagent has been dispensed by 19, and the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 with the dilution liquid is measured by the measurement unit 29. Make measurement and generate confirmation data.

  The performance confirmation unit 50 dispenses the sample dispensed by the sample dispensing probe 19 of the analysis unit 10 based on the confirmation parameter input from the operation unit 80 and the confirmation data generated by the analysis unit 10. Confirm. Here, the quality of the dispensing accuracy and dispensing accuracy is determined using the confirmation data.

  First, determination of dispensing accuracy will be described. In this case, for example, a variation coefficient that is a value indicating variation is obtained by processing a plurality of pieces of confirmation data. If the variation coefficient is within the allowable range set as the confirmation parameter, the sample dispensing probe 19 determines that the sample is being dispensed normally. When the coefficient of variation is out of the allowable range, it is determined that the sample dispensing performance of the sample dispensing probe 19 is degraded.

  Next, determination of dispensing accuracy will be described. In this case, the confirmation reagent dispensed in the reaction container 17 is measured by the measurement unit 29 to generate reference data, and the confirmation reagent dispensed in the reaction container 17 after the measurement is sampled into the sample dispensing probe 19. To dispense. Then, the dilution rate of the confirmation reagent is calculated from the amount of the diluent discharged from the first reagent dispensing probe 21 set as the confirmation parameter and the amount of the confirmation reagent dispensed by the sample dispensing probe 19 to confirm The value which shows the deviation with respect to the reference | standard data of the data which multiplied the dilution data for the data for use is obtained. And it determines with the dispensing performance of the said sample falling when the value which shows deviation has remove | deviated from the preset tolerance | permissible_range.

  The data processing unit 60 generates the calibration data and analysis data of each inspection item by processing the standard data and the test data output from the measurement unit 29 of the analysis unit 10, and the calculation unit 61 generates the data. And a data storage unit 62 for storing standard data and analysis data.

  The calculation unit 61 generates calibration data indicating the relationship between the standard data output from the measurement unit 29 and standard values preset in the standard sample of the standard data, and outputs the generated calibration data to the output unit 70. The data is stored in the data storage unit 62.

  In addition, the calibration data of the test item corresponding to the test data output from the measurement unit 29 is read from the data storage unit 62, and expressed by the concentration value and the enzyme activity value from the test data using the read calibration data. Generate analytical data. The generated analysis data is output to the output unit 70 and stored in the data storage unit 62.

  The data storage unit 62 includes a memory device such as a hard disk, and stores calibration data output from the calculation unit 61 for each inspection item. In addition, the analysis data of each inspection item output from the calculation unit 61 is stored for each test sample.

  The output unit 70 includes a printing unit 71 that prints out the calibration data and analysis data output from the calculation unit 61 of the data processing unit 60 and a display unit 72 that displays the output. The printing unit 71 includes a printer or the like, and prints the calibration data and analysis data output from the calculation unit 61 on printer paper or the like according to a preset format.

  The display unit 72 includes a monitor such as a CRT or a liquid crystal panel, and displays calibration data and analysis data output from the calculation unit 61. Also, an analysis parameter setting screen for setting analysis parameters such as the reagent names of the first and second reagents for analyzing each inspection item to be inspected, the dispensing amounts of the first and second reagents, and the dispensing amount of the sample. Is displayed. Further, a re-inspection parameter setting screen for setting re-inspection parameters such as an analysis range for determining whether or not to re-inspect each inspection item is displayed. In addition, an inspection item setting screen for setting identification information such as a name and ID for identifying the test sample and an inspection item to be tested is displayed for each test sample. In addition, a performance confirmation parameter setting screen for setting confirmation parameters for confirming the dispensing performance is displayed.

  The operation unit 80 includes input devices such as a keyboard, a mouse, a button, and a touch key panel. Then, input for setting analysis parameters and re-inspection parameters for each inspection item, input for setting test identification information and test items of the test sample, input for setting confirmation parameters, and the like are performed.

  The system control unit 90 includes a CPU and a storage circuit, and receives a command signal input by an operation from the operation unit 80, information on analysis parameters and re-inspection parameters of each inspection item, information on test identification information and information on inspection items, and confirmation After the input information such as the parameter information is stored in the storage circuit, the analysis control unit 41, the performance confirmation unit 50, the data processing unit 60 and the output unit 70 are controlled based on the input information to control the entire system. To do.

Next, referring to FIG. 1 to FIG. 3, the performance confirmation parameter setting screen on which the confirmation parameter is displayed on the display unit 72, the analysis unit 10 based on the confirmation parameter displayed on the performance confirmation parameter setting screen, and An example of the operation of the performance confirmation unit 50 will be described.
FIG. 3 is a diagram showing an example of the performance confirmation parameter setting screen displayed on the display unit 72. The performance confirmation parameter setting screen 73 displays the confirmation reagent set in the “dispensing condition” column for setting a confirmation reagent and the like and the “dispensing condition” column in the measuring unit 29 of the analyzing unit 10. A column of “Measurement conditions” for setting the conditions to be measured, and “Judgment criteria” for setting an allowable range for determining whether or not the sample is being dispensed normally by the sample dispensing probe 19 Column. Each confirmation parameter stored in the storage circuit of the system control unit 90 is displayed in each column by setting input from the operation unit 80.

  The “dispensing conditions” column includes the “reagent name” column for setting the confirmation reagent and the first or second reagent that is the confirmation reagent set in the “reagent name” column as the first or second. The “reagent amount” column for setting the amount to be dispensed into the reaction container 17 by the second reagent dispensing probes 21 and 25 is separated from the confirmation reagent dispensed into the reaction container 17 by the sample dispensing probe 19. Column of “sample amount” for setting the amount to be dispensed into the reaction container 17.

  In the “reagent name” column, the reagent name is set with the first or second reagent showing high absorbance at a wavelength detectable by the measurement unit 29 as a confirmation reagent. Here, “A1” indicating that the reagent name of the first reagent of the inspection item A selected and set from the reagent names of the inspection items set as the analysis parameters is displayed.

  Thus, by selecting from the reagents of each inspection item used in the inspection, the selected reagent can be used as a confirmation reagent, so without preparing a sample or the like for performance confirmation, Performance confirmation can be executed.

  In the “reagent amount” column, an amount greater than a predetermined amount, which is the amount that can be measured by the measurement unit 29, is provided in the reaction container 17 dispensed by the first or second reagent dispensing probes 21 and 25. Set. Then, “80” indicating that the amount of the reagent for checking the reagent name set in the “reagent name” column to be dispensed into the reaction container 17 is, for example, 80 μL is displayed.

  With this setting, the first reagent dispensing probe 21 dispenses 80 μL of the confirmation reagent into the reaction container 17, and when the performance confirmation unit 50 determines the dispensing accuracy, the measuring unit 29 is placed in the reaction container 17. The reference reagent is generated by measuring the confirmation reagent.

  In the “sample amount” column, a small amount is set when the sample in the sample container 11 can be dispensed into the reaction container 17 by the sample dispensing probe 19. The amount of the confirmation reagent in the reaction vessel 17 dispensed by the first or second reagent dispensing probes 21 and 25 is dispensed to another reaction vessel 17 by the sample dispensing probe 19 is, for example, 2.0 μL. “2.0” is displayed to indicate that it exists.

  With this setting, the sample dispensing probe 19 dispenses 2.0 μL of the confirmation reagent among the confirmation reagents in the reaction container 17 dispensed by the first reagent dispensing probe 21 to another reaction container 17. To do.

  The “measurement condition” column is set in the “wavelength” column and the “wavelength” column for setting the wavelength of the measurement unit 29 that measures the confirmation reagent set in the “reagent name” column. The column of “Lower limit” for setting the lower limit value of the reference data at the wavelength, the column of “Measurement number” for setting the number of measurements, and the reaction vessel 17 dispensed by the sample dispensing probe 19 And a “diluent amount” column for setting the amount of a diluting solution for diluting the confirmation reagent.

  In the “wavelength” column, the wavelength at which the confirmation reagent exhibits high absorbance is selected and set from wavelengths that can be detected by the measurement unit 29. Here, out of the light transmitted through the confirmation reagent in the reaction container 17 dispensed by the first or second reagent dispensing probes 21 and 25, the wavelength detected by the detector of the measurement unit 29 is, for example, 340 nm. “340” is displayed.

  In the “Lower limit value” column, an allowable lower limit value of the reference data at the wavelength set in the “Wavelength” column in order to determine whether or not the reagent accommodated in the reagent container 13 can be used as a confirmation reagent. Set. Then, “1.2” indicating that the allowable lower limit of the absorbance of the confirmation reagent is 1.2 Abs is displayed.

  With this setting, the measurement unit 29 measures the confirmation reagent in the reaction container 17 and generates reference data indicating the absorbance of the confirmation reagent at 340 nm. In addition, the performance confirmation unit 50 continues the performance confirmation when the reference data is 1.2 Abs or more, and stops the confirmation of the dispensing performance using the confirmation reagent when the reference data is lower than 1.2 Abs. To do.

  In the column of “Diluted liquid amount”, the confirmation reagent in the reaction container 17 dispensed by the sample dispensing probe 19 is discharged into the reaction container 17 in order to obtain an amount that can be measured by the measurement unit 29. Set the amount of dilution to dilute the reagent for confirmation. Then, “78” indicating that the amount of the diluent for diluting the confirmation reagent in the reaction container 17 dispensed by the sample dispensing probe 19 is 78 μL, for example, is displayed.

  With this setting, the first reagent dispensing probe 21 discharges the diluent to the reaction container 17 into which the confirmation reagent has been dispensed by the sample dispensing probe 19. In addition, the measurement unit 29 measures the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 with the diluent, and generates confirmation data indicating the absorbance of the diluted reagent at 340 nm. Further, the performance confirmation unit 50 calculates the dilution rate of the confirmation reagent from the amounts set in the “sample amount” and “diluted solution amount” columns.

  In the “number of times of measurement” column, the number of times the sample dispensing probe 19 dispenses the confirmation reagent of the amount set in the “sample amount” column is set. Then, “5” indicating that the number of times of dispensing of the confirmation reagent by the sample dispensing probe 19 is 5 is displayed.

  The “Judgment Criteria” column has an “Accuracy” column for setting an allowable range for determining whether the dispensing accuracy is good and an “Accuracy” column for setting an allowable range for determining the accuracy of dispensing accuracy. Consists of.

  In the column of “Accuracy”, the variation when the confirmation reagent is dispensed a plurality of times by the sample dispensing probe 19 is within an allowable range when the variation coefficient is 1% or less, and is larger than 1%. In this case, “≦ 1” indicating that the value is outside the allowable range is displayed.

  With this setting, the performance confirmation unit 50 calculates an average value and a standard deviation using a plurality of confirmation data generated by measuring a plurality of dilution reagents, obtains a variation coefficient from the average value and the standard deviation, And the quality of the dispensing accuracy is determined based on the allowable range set in the column of “Accuracy”. When the coefficient of variation is within the allowable range, it is determined that the sample is dispensed normally, and when the coefficient of variation is outside the allowable range, it is determined that the sample dispensing performance is degraded. To do.

  In the column of “Accuracy”, the value indicating the deviation when the confirmation reagent is dispensed one or more times by the sample dispensing probe 19 is within the allowable range when the value is within the range of 100 ± 5%. When it is out of the range of ± 5%, “± 5” indicating that it is out of the allowable range is displayed.

  With this setting, the performance confirmation unit 50 obtains a value expressed as a percentage indicating a deviation from the reference data of data obtained by multiplying the confirmation data generated by the measurement of the diluted reagent by the dilution rate of the confirmation reagent, and indicates the deviation. Whether or not the dispensing accuracy is good is determined based on the value and the allowable range set in the column of “Accuracy”. Then, it is determined that the dispensing performance of the sample is deteriorated only when the value indicating the deviation is out of the allowable range.

  In addition, since the amount of the confirmation reagent dispensed by the sample dispensing probe 19 is extremely large compared to the discharge amount of the diluent, an error factor in dispensing accuracy and dispensing accuracy depends on the dispensing of the sample dispensing probe 19. Error factors account for the majority.

  In this way, by setting the confirmation parameter, any reagent other than the time when the inspection is performed by the analysis unit 10 such as the periodic inspection before the start of the inspection, after the end of the inspection, using the reagent used for the inspection is used. Performance confirmation can be executed at the timing.

  Next, an example of the operation of each analysis unit of the analysis unit 10 will be described with reference to FIGS.

First, an analysis unit related to each dispensing of the sample dispensing probe 19, the first reagent dispensing probe 21, and the second reagent dispensing probe 25 will be described.
FIG. 4 is a diagram showing an example of the configuration of a sample dispensing unit related to dispensing of the sample dispensing probe 19. The sample dispensing unit 38 includes a tube 381 having one end connected to the upper end of the sample dispensing probe 19, a syringe 382 having an upper end connected to the other end of the tube 381, and a lower end of the syringe 382. It is constituted by a plunger 383 that seals an opening provided in the section and is slidable in the direction of the arrow L1 and the direction of the arrow L2.

  In addition, the sample dispensing unit 38 includes a pump 385 for supplying and filling water as a pressure transmission medium stored in the tank 384 into the syringe 382, the tube 381, and the sample dispensing probe 19, and the syringe 382 and the pump. And an open / close valve 386 that opens and closes a flow path communicating between the two.

  In the sample dispensing, the drive unit 40 sucks and drives the plunger 383 in the L1 direction while the flow path between the syringe 382 and the pump 385 is closed by the on-off valve 386 driven and controlled by the analysis control unit 41. As a result, the sample dispensing probe 19 sucks the sample in the sample container 11 and the confirmation reagent in the reaction container 17. Further, when the drive unit 40 drives the plunger 383 to discharge in the L2 direction, the sample dispensing probe 19 discharges the aspirated sample and confirmation reagent into the reaction container 17.

  Similar to the sample dispensing unit 38, the first reagent dispensing probe 21 is connected to a first reagent dispensing unit including a tube, a syringe, a plunger, a tank, a pump, and an on-off valve. Then, the first reagent and the diluent in the reagent container 13 are sucked by the suction drive of the drive unit 40. Further, the aspirated first reagent and diluent are discharged into the reaction container 17 by the discharge drive of the drive unit 40.

  Similarly to the sample dispensing unit 38, the second reagent dispensing probe 25 is connected to a second reagent dispensing unit including a tube, a syringe, a plunger, a tank, a pump, and an on-off valve. Then, the second reagent in the reagent container 13 is aspirated by the suction drive of the drive unit 40. Further, the aspirated second reagent is discharged into the reaction container 17 by the discharge drive of the drive unit 40.

  FIG. 5 is a diagram illustrating an example of each stop position of each reaction vessel 17 and the position of the measurement unit 29 held in the reaction table 18. When the start of inspection is input from the operation unit 80, each reaction container 17 rotates and moves in the direction of arrow R1 every cycle time, and the cleaning position W, the sample discharge position Pa, and the first reagent discharge are different from those before the movement. It stops at each stop position including the position Pb, the first stirring position Pc, the diluted sample suction position Pf, the second reagent discharge position Pd, and the second stirring position Pe. And it stops at the same stop position every round time which is longer than one cycle time.

  Here, each reaction container 17 is rotationally moved in the R1 direction every cycle time by the rotational drive of the reaction table 18 at an angle θ, and is a reaction located at an angle of 90 °, for example, in the R1 direction from the position before the movement. It stops at the position of the reaction vessel 17 adjacent to the vessel 17 in the direction opposite to the R1 direction.

  The measurement unit 29 includes a light source and a detector arranged across the inner and outer circumferences of each reaction container 17, and irradiates each reaction container 17 that passes through the measurement position Pm with each rotation of the reaction table 18. Then, light in a plurality of wavelength ranges transmitted through the mixed solution containing the standard sample and the test sample in each reaction container 17 is detected to generate standard data and test data represented by absorbance.

  Next, with reference to FIG. 1 to FIG. 6, an example of an inspection performed by the analysis unit 10 and an operation when it is determined that a re-inspection is necessary in this inspection will be described.

FIG. 6 is a diagram showing the cleaning position of the cleaning nozzle 30 and the dispensing positions of the sample dispensing probe 19, the first reagent dispensing probe 21, and the second reagent dispensing probe 25.
The cleaning nozzle 30 includes, for example, seven first to seventh cleaning nozzles 31 to 37, and is arranged to be movable in the vertical direction at the cleaning position W by the vertical drive of the drive unit 40. The first to seventh cleaning nozzles 31 to 37 stop at the upper stop position when the reaction table 18 is rotating, and move downward when the reaction table 18 is stopped to move to the first to seventh cleaning nozzles 31 to 37. The reaction container 17 stopped at the seventh cleaning positions W1 to W7 is cleaned.

  The reaction container 17 that has stopped at the seventh cleaning position W7 and has finished cleaning by the first to seventh cleaning nozzles 31 to 37 stops at the sample discharge position Pa, for example, at the second cycle after two cycle times have passed. The sample dispensing probe 19 is a reaction container at the sample discharge position Pa for analysis of, for example, the inspection item A, in which the test sample collected from the test object P of the sample container 11 held on the sample table 12 is set to this sample. Dispense into 17.

  In the third cycle after one cycle time has elapsed from the second cycle, the reaction container 17 into which the sample has been dispensed at the sample discharge position Pa in the second cycle stops at the first reagent discharge position Pb. The first reagent dispensing probe 21 dispenses the first reagent for analysis of the test item A in the reagent container 13 held in the reagent rack 14 in the reagent storage 15 into the reaction container 17 at the first reagent discharge position Pb. .

  In the fifth cycle, in which two cycle times have elapsed from the third cycle, the reaction vessel 17 into which the first reagent has been dispensed at the first reagent discharge position Pb in the third cycle stops at the first stirring position Pc. The first stirrer 23 stirs the mixed solution of the sample and the first reagent in the reaction container 17 at the first stirring position Pc.

  In the sixth cycle after one cycle time has elapsed from the fifth cycle, the reaction vessel 17 that has been stirred at the first stirring position Pc in the fifth cycle stops at the diluted sample suction position Pf.

  In the d cycle when a predetermined cycle time has elapsed from the sixth cycle, the reaction vessel 17 stopped at the diluted sample suction position Pf in the fifth cycle stops at the second reagent discharge position Pd. The second reagent dispensing probe 25 dispenses the second reagent for analysis of the inspection item A in the reagent container 13 held in the reagent rack 14 in the reagent storage 16 into the reaction container 17 at the second reagent discharge position Pd. To do.

  In the (d + 1) cycle when one cycle time has elapsed from the d cycle, the reaction container 17 into which the second reagent has been dispensed at the second reagent discharge position Pd in the d cycle stops at the second stirring position Pe. The second stirrer 27 stirs the mixed solution of the sample, the first reagent, and the second reagent in the reaction container 17 at the second stirring position Pe.

  The measurement unit 29 measures the liquid mixture in the reaction vessel 17 that passes through the measurement position Pm between the stop at the second stirring position Pe and the stop at the cleaning position W in the (d + 1) cycle. Generate data. Then, the calculation unit 61 of the data processing unit 60 generates analysis data of the inspection item A based on the test data generated by the measurement unit 29.

  The reaction vessel 17 stirred at the second stirring position Pe in the (d + 1) cycle has the first to seventh washing positions in the g cycle, the (g + 4) cycle,..., The (g + 24) cycle. Stop at W1 to W7. The first to seventh cleaning nozzles 31 to 37 clean the reaction vessel 17 that has been stirred at the second stirring position Pe in the (d + 1) cycle.

  The system control unit 90 determines whether to perform reinspection based on the analysis data generated by the arithmetic unit 61 and the reinspection parameter. When it is determined that reexamination is unnecessary, the analysis control unit 41 is instructed to end the analysis of the inspection item A of the subject P. If it is determined that reexamination is necessary, the analysis control unit 41 is instructed to reexamine the inspection item A of the subject P.

  If the test sample dispensed into the reaction container 17 at the sample discharge position Pa in the second cycle is for retesting of the inspection item A by the retest instruction, the first reagent dispensing probe in the third cycle 21 sucks the diluted solution in the reagent container 13 held in the reagent rack 14 and discharges the diluted liquid to the reaction container 17 at the first reagent discharge position Pb. In the fourth cycle, the first stirrer 23 moves to the first stirring position Pc. In the sixth cycle, the sample dispensing probe 19 stirs the diluted sample in the reaction vessel 17 at the diluted sample suction position Pf for retesting. Dispense into the reaction container 17 at the sample discharge position Pa. When the reaction container 17 containing the diluted sample stops at the first reagent discharge position Pb, the first reagent dispensing probe 21 transfers the first reagent for analysis of the test item A to the reaction container 17 at the first reagent discharge position Pb. Dispense. The first stirrer 23 stirs the mixed liquid in the reaction container 17 when the reaction container 17 containing the mixed liquid of the diluted sample and the first reagent stops at the first stirring position Pc. The second reagent dispensing probe 25 dispenses the second reagent for analysis of the test item A when the reaction container 17 containing the mixed solution of the diluted sample and the first reagent stops at the second reagent discharge position Pd. The second stirring bar 27 stirs the liquid mixture in the reaction container 17 when the reaction container 17 containing the diluted sample, the mixed liquid of the first reagent and the second reagent stops at the second stirring position Pe. The measurement unit 29 measures the liquid mixture in the reaction vessel 17 that passes through the measurement position Pm between the time when it stops at the second stirring position Pe and the time when it stops at the cleaning position W, and generates the absorbance. Generates the analysis data of the inspection item A based on the absorbance generated by the measurement unit 29.

  In this way, when the analysis data obtained by the first measurement is out of the analysis range at a high concentration, for example, the sample is again dispensed into the reaction vessel 17 and the dispensed sample in the reaction vessel 17 is removed. It is possible to perform a re-inspection by diluting with the diluent and diluting the diluted sample in the reaction vessel 17 diluted with the diluent into another reaction vessel 17.

  Hereinafter, an example of an operation for confirming the performance of the automatic analyzer 100 will be described with reference to FIGS. 1 to 8.

  The confirmation parameters displayed on the performance confirmation parameter setting screen 73 of FIG. 3 are stored in the storage circuit of the system control unit 90 of the automatic analyzer 100. When the start of performance confirmation is input from the operation unit 80, the system control unit 90 performs performance confirmation by the pass / fail determination of the dispensing accuracy and the dispensing accuracy to the analysis control unit 41, the performance confirmation unit 50, and the output unit 70. Instruct. The analysis control unit 41 controls the measurement unit 29 and the drive unit 40 of the analysis unit 10 based on the confirmation parameters stored in the storage circuit of the system control unit 90.

  In this way, by setting the confirmation parameters in advance, it is not necessary to prepare a sample or the like for confirmation, and the automatic analyzer 100 can be simply operated by performing an input operation for starting confirmation performance. Confirmation of dispensing performance can be executed.

  7 and 8 are diagrams showing an example of operations of the cleaning nozzle 30, each reaction container 17, the first reagent dispensing probe 21, and the sample dispensing probe 19 in the performance check. And each reaction container 17 operate | moves similarly to the operation | movement shown in FIG. Further, the cleaning nozzle 30 and the first reagent dispensing probe 21 operate in the same manner as in the case of inspection and re-inspection. Further, the sample dispensing probe 19 operates in the same manner as in the retest.

  The reaction container 17 at the seventh cleaning position W7 that has been cleaned by the cleaning nozzle 30 stops at the first reagent discharge position Pb, for example, at the third cycle after the elapse of three cycle times. The first reagent dispensing probe 21 aspirates the first reagent for analysis of the test item A in the reagent container 13 held in the reagent rack 14 in the reagent container 15 as a confirmation reagent, and the first reagent dispensing probe 21 at the first reagent discharge position Pb. The first dispensing to discharge into the reaction vessel 17 is performed.

  In the fourth cycle, the first reagent dispensing probe 21 aspirates the confirmation reagent in the reagent container 13 and performs the second dispensing to be discharged to the reaction container 17 stopped at the first reagent discharge position Pb after cleaning. Do.

  In the fifth cycle, the reaction vessel 17 into which the confirmation reagent has been dispensed in the first reagent discharge position Pb in the third cycle stops at the first stirring position Pc. In addition, the first reagent dispensing probe 21 performs the third dispensing to suck the confirmation reagent in the reagent container 13 and discharge it to the reaction container 17 stopped at the first reagent discharging position Pb after the cleaning.

  The measurement unit 29 stops at the first stirring position Pc in the fifth cycle, and then measures the confirmation reagent in the reaction vessel 17 that passes through the measurement position Pm to generate the first reference data. The eye reference data is output to the performance confirmation unit 50. The performance confirmation unit 50 continues the performance confirmation when the first reference data is greater than or equal to the allowable lower limit value, and confirms the dispensing performance using the confirmation reagent when the reference data is lower than the allowable lower limit value. To stop.

  The analysis control unit 41 stops the performance confirmation operation of the analysis unit 10 based on the dispensation confirmation stop of the performance confirmation unit 50. Further, the system control unit causes the display unit 72 to display abnormality information of the confirmation reagent based on the confirmation stop of the dispensing performance of the performance confirmation unit 50. Hereinafter, an operation when the confirmation reagent is normal will be described.

  In the sixth cycle, the reaction container 17 into which the confirmation reagent has been dispensed at the first reagent discharge position Pb in the fourth cycle stops at the first stirring position Pc. Further, the sample dispensing probe 19 sucks the confirmation reagent in the reaction container 17 in which the first dispensing stopped at the diluted sample suction position Pf, finishes the cleaning, and stops at the sample discharge position Pa. The first dispensing to be discharged into the container 17 is performed. In addition, the first reagent dispensing probe 21 performs the fourth dispensing that sucks the confirmation reagent in the reagent container 13 and discharges it to the reaction container 17 stopped at the first reagent discharge position Pb after the cleaning.

  After stopping at the first stirring position Pc in the sixth cycle, the measurement unit 29 measures the confirmation reagent in the reaction vessel 17 that passes through the measurement position Pm, and generates second reference data. The eye reference data is output to the performance confirmation unit 50.

  In the seventh cycle, the reaction container 17 into which the confirmation reagent has been dispensed at the first reagent discharge position Pb in the fifth cycle stops at the first stirring position Pc. In addition, the sample dispensing probe 19 sucks the confirmation reagent in the reaction container 17 that has been dispensed for the second time to stop at the diluted sample suction position Pf, finishes the cleaning, and stops at the sample discharge position Pa. The second dispensing to be discharged into the container 17 is performed. Further, the first reagent dispensing probe 21 sucks the diluted solution in the reagent container 13, and the first dispensing reagent 19 is dispensed by the sample dispensing probe 19 which stops at the first reagent discharge position Pb. Discharge into the reaction vessel 17.

  As described above, the operation of dispensing the sample in the sample container 11 into the reaction container 17 by the sample dispensing probe 19 at the time of inspection is performed in the reaction container 17 at the diluted sample suction position Pf using the operation at the time of re-inspection. The sample dispensing performance can be confirmed by replacing it with the operation of dispensing the confirmation reagent in the reaction container 17 at the sample discharge position Pa.

  After the plunger of the first reagent dispensing unit is driven to be sucked by the drive unit 40, each of the syringe and tube of the first reagent dispensing unit and the first reagent dispensing probe 21 is pumped by the pump of the first reagent dispensing unit. The inside is filled with a pressure transmission medium. Then, the plunger may be driven to be driven by the driving unit 40 so that the water as the pressure transmission medium is discharged from the first reagent dispensing probe 21 as a diluent for diluting the confirmation reagent.

  After stopping at the first stirring position Pc in the seventh cycle, the measurement unit 29 measures the confirmation reagent in the reaction vessel 17 passing through the measurement position Pm, and generates the third reference data. The eye reference data is output to the performance confirmation unit 50.

  In the eighth cycle, the reaction container 17 into which the confirmation reagent has been dispensed at the first reagent discharge position Pb in the sixth cycle stops at the first stirring position Pc. Further, the sample dispensing probe 19 sucks the confirmation reagent in the reaction container 17 that has been dispensed for the third time, which stops at the diluted sample suction position Pf, finishes the cleaning, and stops at the sample discharge position Pa. The third dispensing to be discharged into the container 17 is performed. Further, the first reagent dispensing probe 21 sucks the diluted solution in the reagent container 13 and the second dispensing of the confirmation reagent is performed by the sample dispensing probe 19 which stops at the first reagent discharge position Pb. Discharge into the reaction vessel 17.

  After stopping at the first stirring position Pc in the eighth cycle, the measurement unit 29 measures the confirmation reagent in the reaction container 17 in which the fourth dispensing that has passed through the measurement position Pm is performed, and measures the fourth. The reference data is generated, and the fourth reference data is output to the performance confirmation unit 50.

  In the ninth cycle, the reaction vessel 17 in which the diluent is discharged at the first reagent discharge position Pb in the seventh cycle stops at the first stirring position Pc. The first stirrer 23 stirs the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 at the first stirring position Pc with the diluent. In addition, the sample dispensing probe 19 sucks the confirmation reagent in the reaction container 17 in which the fourth dispensing stopped at the diluted sample suction position Pf, finishes the cleaning, and stops at the sample discharge position Pa. The fourth dispensing to be discharged into the container 17 is performed. Further, the first reagent dispensing probe 21 sucks the diluted solution in the reagent container 13 and the third dispensing of the confirmation reagent is performed by the sample dispensing probe 19 which stops at the first reagent discharge position Pb. Discharge into the reaction vessel 17.

  After the stirring is performed at the first stirring position Pc in the ninth cycle, the measurement unit 29 measures the diluted reagent in the reaction container 17 passing through the measurement position Pm, and generates the first confirmation data, The first confirmation data is output to the performance confirmation unit 50.

  In the tenth cycle, the reaction container 17 in which the diluent is discharged at the first reagent discharge position Pb in the eighth cycle stops at the first stirring position Pc. The first stirrer 23 stirs the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 at the first stirring position Pc with the diluent. Further, the first reagent dispensing probe 21 sucks the diluted solution in the reagent container 13 and the fourth dispensing of the confirmation reagent is performed by the sample dispensing probe 19 which stops at the first reagent discharge position Pb. Discharge into the reaction vessel 17.

  After the stirring is performed at the first stirring position Pc in the 10th cycle, the measurement unit 29 measures the diluted reagent in the reaction vessel 17 that passes through the measurement position Pm, and generates second confirmation data. The second confirmation data is output to the performance confirmation unit 50.

  In the eleventh cycle, the reaction vessel 17 in which the diluent is discharged at the first reagent discharge position Pb in the ninth cycle stops at the first stirring position Pc. The first stirrer 23 stirs the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 at the first stirring position Pc with the diluent. The first reagent dispensing probe 21 sucks the confirmation reagent in the reagent container 13 and performs the fifth dispensing to finish the cleaning and discharge the reaction reagent to the reaction container 17 that stops at the first reagent discharge position Pb.

  The measurement unit 29 measures the diluted reagent in the reaction container 17 that passes through the measurement position Pm after stirring is performed at the first stirring position Pc in the eleventh cycle, and generates third check data. The third confirmation data is output to the performance confirmation unit 50.

  In the twelfth cycle, the reaction vessel 17 in which the diluent is discharged at the first reagent discharge position Pb in the tenth cycle stops at the first stirring position Pc. The first stirrer 23 stirs the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 at the first stirring position Pc with the diluent.

  The measurement unit 29 measures the diluted reagent in the reaction container 17 passing through the measurement position Pm after stirring is performed at the first stirring position Pc in the twelfth cycle, and generates fourth confirmation data. The fourth confirmation data is output to the performance confirmation unit 50.

  In the thirteenth cycle, the reaction container 17 into which the confirmation reagent has been dispensed at the first reagent discharge position Pb in the eleventh cycle stops at the first stirring position Pc. Then, after stopping at the first stirring position Pc in the thirteenth cycle, the measurement unit 29 measures five confirmation reagents in the reaction container 17 in which the fifth dispensing that passes through the measurement position Pm has been performed. The eye reference data is generated, and the fifth reference data is output to the performance confirmation unit 50.

  In the 14th cycle, the sample dispensing probe 19 aspirates the confirmation reagent in the reaction container 17 that has been dispensed for the fifth time and stops at the diluted sample aspirating position Pf. Dispensing the fifth time to discharge into the reaction vessel 17 to be stopped.

  In the fifteenth cycle, the first reagent dispensing probe 21 sucks the diluted solution in the reagent container 13 and the fifth dispensing of the confirmation reagent is performed by the sample dispensing probe 19 that stops at the first reagent discharge position Pb. It discharges to the reaction container 17 performed.

  In the 17th cycle, the reaction container 17 in which the diluent is discharged at the first reagent discharge position Pb in the 15th cycle stops at the first stirring position Pc. The first stirrer 23 stirs the diluted reagent obtained by diluting the confirmation reagent in the reaction container 17 at the first stirring position Pc with the diluent.

  The measurement unit 29 measures the diluted reagent in the reaction vessel 17 passing through the measurement position Pm after stirring is performed at the first stirring position Pc in the 17th cycle, and generates fifth confirmation data. The fifth confirmation data is output to the performance confirmation unit 50.

  The performance confirmation unit 50 calculates an average value and a standard deviation from the five pieces of confirmation data, obtains a variation coefficient from the average value and the standard deviation, and determines whether the dispensing accuracy is good based on the variation coefficient and the dispensing accuracy allowable range. Determine. When the coefficient of variation is within the allowable range, it is determined that the sample is dispensed normally, and when the coefficient of variation is outside the allowable range, it is determined that the sample dispensing performance is degraded. To do.

  Further, the performance confirmation unit 50 calculates an average value of the five reference data and an average value of the five confirmation data. Next, a value obtained by multiplying the average value of the confirmation data by the dilution ratio of the confirmation reagent and expressed as a percentage indicating the deviation from the average value of the reference data is obtained, and based on the value indicating the deviation and the allowable range of the dispensing accuracy. To determine the accuracy of dispensing. And when the value which shows deviation has remove | deviated from the tolerance | permissible_range, it determines with the sample dispensing performance falling.

  Thus, the determination of the dispensing performance of the sample can be performed by determining whether the dispensing accuracy or the dispensing accuracy is good or bad.

  In addition, it is not limited to the said embodiment, You may make it implement the operation | movement of the performance confirmation in the analysis part 10 by the operation | movement demonstrated below. Each reaction vessel 17 is moved in the direction opposite to the R1 direction and the R1 direction shown in FIG. 5, and stopped at any stop position shown in FIG. Then, after washing at least six reaction vessels 17 with the washing nozzle 30, the first reagent dispensing probe 21 causes the first reagent dispensing probe 19 to supply a confirmation reagent in an amount that can be dispensed five times. Dispense into container 17. Then, the sample dispensing probe 19 is made to suck the confirmation reagent in the reaction container 17 and is discharged into the second reaction container 17 that has been washed for the first time. Next, the sample dispensing probe 19 sucks the confirmation reagent in the reaction container 17 and performs the second dispensing to be discharged into the washed third reaction container 17. Similarly, the third to fifth dispensing is performed. The diluted solution is discharged from the first reagent dispensing probe 21 to the five reaction containers 17 into which the confirmation reagents have been dispensed by the sample dispensing probe 19, and the diluted reagents in the five reaction containers 17 are sent to the measuring unit 29. Let me measure.

  In the case of confirming the performance by this operation, the plunger 383 of the sample dispensing unit 38 is driven to suck in the L1 direction from the time of sample dispensing, and then the syringe 382, the tube 381, and the sample dispensing probe 19 are pumped by the pump 385. Each interior is filled with a pressure transmission medium. Then, the plunger 383 is driven to be discharged by the drive unit 40, and water as a pressure transmission medium may be discharged from the sample dispensing probe 19 as a diluent for diluting the confirmation reagent.

  According to the embodiment described above, the reagent selected from the reagents of each inspection item used in the inspection is input to the reaction container 17 as the confirmation reagent by the input operation for setting the confirmation parameter and starting the performance confirmation. The confirmation reagent dispensed into the reaction vessel 17 is dispensed by the sample dispensing probe 19 into a reaction vessel 17 other than the reaction vessel 17, and the reaction vessel 17 is dispensed by the sample dispensing probe 19. The confirmation reagent can be diluted and measured. And based on the data for confirmation obtained by the measurement, the dispensing performance of the sample by the sample dispensing probe 19 can be confirmed.

  Thus, it is not necessary to prepare a sample or the like for confirmation at any timing other than when the analysis unit 10 is inspected, and it can be quickly and easily performed by an input operation for starting the confirmation performance. Dispensing performance can be confirmed.

  Although several embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalents thereof.

DESCRIPTION OF SYMBOLS 10 Analysis part 11 Sample container 13 Reagent container 17 Reaction container 19 Sample dispensing probe 21 1st reagent dispensing probe 25 2nd reagent dispensing probe 29 Measuring part 41 Analysis control part 50 Performance confirmation part

Claims (8)

A plurality of reaction containers, a sample dispensing probe for dispensing a sample contained in a sample container into the reaction container, a reagent dispensing probe for dispensing a reagent contained in a reagent container into the reaction container, and the reaction container In an automatic analyzer equipped with a measuring unit for measuring a dispensed sample and reagent mixture,
The reagent contained in the reagent container is dispensed as a confirmation reagent into the reaction container by the reagent dispensing probe, and the confirmation reagent dispensed in the reaction container is dispensed by the sample dispensing probe other than the reaction container. Dispense into the reaction container, and discharge the diluent from the reagent dispensing probe or the sample dispensing probe into the reaction container into which the confirmation reagent has been dispensed by the sample dispensing probe. A value indicating variation is obtained from a plurality of confirmation data generated by measuring the diluted reagent diluted with the diluent by the measurement unit , and the value is within a preset allowable range. When it is determined that the sample is dispensed normally and the value is out of the allowable range, the sample dispensing performance of the sample by the sample dispensing probe is degraded. Automatic analyzer characterized by comprising determining performance check unit and. The measurement unit measures the confirmation reagent in the reaction container dispensed by the reagent dispensing probe and generates reference data,
The performance confirmation unit is configured to use the dilution liquid based on a preset amount of the diluent dispensed from the reagent dispensing probe or the sample dispensing probe and a confirmation reagent dispensed by the sample dispensing probe. The dilution factor of the confirmation reagent to be diluted is calculated, a value indicating a deviation of the reference data from the data obtained by multiplying the confirmation data by the dilution factor is obtained, and the value is out of the preset allowable range. The automatic analyzer according to claim 1, wherein in this case, it is determined that the dispensing performance of the sample by the sample dispensing probe is degraded.   A plurality of reaction containers, a sample dispensing probe for dispensing a sample contained in a sample container into the reaction container, a reagent dispensing probe for dispensing a reagent contained in a reagent container into the reaction container, and the reaction container In an automatic analyzer equipped with a measuring unit for measuring a dispensed sample and reagent mixture,
  The reagent contained in the reagent container is dispensed as a confirmation reagent into the reaction container by the reagent dispensing probe, and the confirmation reagent dispensed in the reaction container is dispensed by the sample dispensing probe other than the reaction container. Dispense into the reaction container, and discharge the diluent from the reagent dispensing probe or the sample dispensing probe into the reaction container into which the confirmation reagent has been dispensed by the sample dispensing probe. Provided with a performance confirmation unit for confirming the dispensing performance of the sample by the sample dispensing probe based on confirmation data generated by measuring the diluted reagent diluted with the diluent by the measurement unit ,
  The measurement unit measures the confirmation reagent in the reaction container dispensed by the reagent dispensing probe and generates reference data,
  The performance confirmation unit is configured to use the dilution liquid based on a preset amount of the diluent dispensed from the reagent dispensing probe or the sample dispensing probe and a confirmation reagent dispensed by the sample dispensing probe. The dilution factor of the confirmation reagent to be diluted is calculated, a value indicating a deviation of the reference data from the data obtained by multiplying the confirmation data by the dilution factor is obtained, and the value is out of the preset allowable range. In this case, it is determined that the dispensing performance of the sample by the sample dispensing probe is deteriorated. The reference data is an absorbance equal to or higher than a preset lower limit value at a predetermined wavelength,
4. The automatic analyzer according to claim 2 , wherein the measurement unit includes a light source that irradiates light to the reaction container and a detector that detects light having the predetermined wavelength of the light. 5.   5. The automatic analyzer according to claim 4, wherein the performance confirmation unit stops confirming the dispensing performance of the sample when the reference data is a value smaller than the lower limit value. The amount of the diluent discharged from the reagent dispensing probe or the sample dispensing probe is smaller than the amount of the confirmation reagent dispensed by the sample dispensing probe. The automatic analyzer according to any one of 5. A plurality of reaction containers, a sample dispensing probe for dispensing a sample contained in a sample container into the reaction container, a reagent dispensing probe for dispensing a reagent contained in a reagent container into the reaction container, and the reaction container In the method for confirming the dispensing performance of an automatic analyzer equipped with a measuring unit for measuring a mixture of dispensed sample and reagent,
Dispensing the reagent contained in the reagent container into the reaction container with the reagent dispensing probe as a confirmation reagent,
The confirmation reagent dispensed in the reaction vessel is dispensed into a reaction vessel other than the reaction vessel by the sample dispensing probe,
The diluent is discharged from the reagent dispensing probe or the sample dispensing probe into the reaction container into which the confirmation reagent has been dispensed by the sample dispensing probe,
Measuring the diluted reagent obtained by diluting the confirmation reagent in the reaction vessel with the diluent with the measurement unit to generate confirmation data;
A value indicating variation is obtained from a plurality of the confirmation data , and when the value is within a preset allowable range, it is determined that dispensing of the sample is performed normally, and the value is within the allowable range A method for confirming the dispensing performance of an automatic analyzer, wherein the performance confirming unit performs a process of determining that the dispensing performance of the sample by the sample dispensing probe is deteriorated when the sample dispensing probe is off .   A plurality of reaction containers, a sample dispensing probe for dispensing a sample contained in a sample container into the reaction container, a reagent dispensing probe for dispensing a reagent contained in a reagent container into the reaction container, and the reaction container In the method for confirming the dispensing performance of an automatic analyzer equipped with a measuring unit for measuring a mixture of dispensed sample and reagent,
Dispensing the reagent contained in the reagent container into the reaction container with the reagent dispensing probe as a confirmation reagent,
The confirmation reagent dispensed in the reaction vessel is dispensed into a reaction vessel other than the reaction vessel by the sample dispensing probe,
The diluent is discharged from the reagent dispensing probe or the sample dispensing probe into the reaction container into which the confirmation reagent has been dispensed by the sample dispensing probe,
Measuring the diluted reagent obtained by diluting the confirmation reagent in the reaction vessel with the diluent with the measurement unit to generate confirmation data;
Measuring the confirmation reagent in the reaction vessel dispensed by the reagent dispensing probe and generating reference data by the measurement unit;
A confirmation reagent diluted with the diluent based on a preset amount of the diluent dispensed from the reagent dispensing probe or the sample dispensing probe and an amount of the confirmation reagent dispensed by the sample dispensing probe The dilution ratio is calculated, a value indicating a deviation of the data obtained by multiplying the confirmation data by the dilution ratio with respect to the reference data is obtained, and the sample dispensing is performed when the value is out of a preset allowable range. A method for confirming the dispensing performance of an automatic analyzer, wherein the performance confirmation unit performs a process of determining that the dispensing performance of the sample by the probe has deteriorated.

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