JP6153520B2 - Skin beauty kit containing cosmetic composition and application method - Google Patents

Description

  The present invention relates to a skin cosmetic kit and a coating method including a cosmetic composition, and more specifically to a skin cosmetic kit and a coating method including a cosmetic composition having an excellent skin cooling effect due to tensile stimulation.

  In general, cosmetics are intended to clean the body, decorate themselves beautifully and attractively, and protect the skin and hair from ultraviolet rays and dryness to prevent aging. With the recent development of industrial society and increase in social activities, the self-consciousness to actively express themselves increases, and the desire to maintain health by taking care of own skin using cosmetics The basic cosmetics in various forms and dosage forms are being researched and developed.

  The elasticity of the skin is shown by elastic fibers composed of elastin present in the dermis layer, and these elastic fibers have a very low elastic modulus like rubber, so that they can be deformed even with a small force. It is easy to be done, and when the force is removed, it easily returns to its original shape. The elastic fiber has a form in which microfibrils are stuck in an amorphous temperament called elastin. Elastin is a protein composed of very unique amino acids found only in elastic fibers of lysine-derived desmosines and isodesmosines. Such desmosine and isodesmosine form cross-links in a long peptide chain, and such a structure gives elastin a rubber-like property.

  In addition, elastic fibers composed of elastin exist together with collagen fibers called collagen, and the elasticity of the skin can be maintained in a state where elastin and collagen are sufficiently present.

  On the other hand, a decrease in the elasticity of the skin is induced by a decrease in the production and destruction of elastin due to a weakening of skin cells (fibroblasts) due to aging or ultraviolet rays. An elastin degrading enzyme is expressed in the skin by ultraviolet rays or the like, and this enzyme degrades normal elastin in the skin, resulting in a decrease in the elasticity of the skin. In addition, the skin elasticity is reduced by the damage and reduction of the protein called collagen, which constitutes 70% or more of the skin dermis. Collagen is also degraded by a degrading enzyme called collagenase induced by ultraviolet rays or the like. Prior patents related to the enhancement of skin elasticity simply suppress the activity of collagenase or the activity of elastase in order to show the effect of improving skin elasticity, and have a limited effect on the dermis layer. As a result, the function cannot be maximized.

  On the other hand, recently, as the climate has become more polar, the interest in products that can lower the temperature of the skin and soothe in the summer is increasing as the summer becomes longer and it becomes hot and humid. In fact, makeup closes the pores of the skin, gives it a hot feeling, and disappears when sweat flows, increasing the need for refreshing cosmetics among women who need to apply makeup every day doing. As a result, products aimed at improving the mood by cooling and calming the skin that has been lit by the heat and tightening the pores have been released as summer products. Specifically, many products using ingredients such as menthol, menthol derivatives, xylitol, etc. as cooling agents have appeared in general cosmetic preparations, but this is insufficient to immediately feel the cooling effect.

International Publication No. 2010/091893 JP-A-8-332113 JP 2009-13129 A US Patent Application Publication No. 2008-63674 Korean Patent No. 10-1032271

  The present invention is excellent in tensile stimulation that cannot be realized by a general application method by applying a cosmetic composition containing arginine and lysine-containing polypeptides as active ingredients to the skin using a skin application device. Another object of the present invention is to provide a skin cosmetic kit and an application method that exhibit a skin cooling effect.

  In order to solve the above-mentioned object, the present invention includes a cosmetic composition containing an arginine and lysine-containing polypeptide as an active ingredient, and a skin application device for applying the cosmetic composition to the skin. Provide skin cosmetic kit.

  In one embodiment of the present invention, the skin application device may be one or more selected from the group consisting of a roller for applying a tensile stimulus, a shiatsu device, and a massage device.

  In one embodiment of the present invention, the skin application device may be roll-on.

  In an embodiment of the present invention, the roll-on may include three or more balls.

  In one embodiment of the present invention, the cosmetic composition may further contain at least one of oat extract and yeast extract.

  In one embodiment of the present invention, the cosmetic composition comprises 0.01 to 2% by weight of arginine and lysine-containing polypeptide, 2 to 6% by weight of oat extract, and yeast extraction, based on the total weight of the composition. The product may contain 0.01 to 0.5% by weight.

  In one embodiment of the present invention, the cosmetic composition may further contain one or more selected from the group consisting of adenosine, retinol and niacinamide.

  In one embodiment of the present invention, the skin cosmetic kit may have one or more selected from the group consisting of a skin cooling application, a skin elasticity enhancement application, and a skin wrinkle improvement application.

  In addition, the present invention includes a step of applying a tensile stimulus to the skin using a skin application device at the same time or after the cosmetic composition containing arginine and lysine-containing polypeptide as active ingredients is applied to the skin. A method for applying a cosmetic composition is provided.

  In one embodiment of the present invention, the application method may include one or more selected from the group consisting of a skin cooling application, a skin elasticity enhancement application, and a skin wrinkle improvement application.

  According to the present invention, a cosmetic composition having an effect of enhancing skin elasticity is applied to the skin using a skin application device that applies a tensile stimulus instead of a general application method. A skin beauty kit and an application method can be provided that generate synergistic effects of the two and exhibit superior skin cooling, skin elasticity enhancement, and skin wrinkle reduction effects.

It is a photograph which shows the change of actin at the time of the tension stimulus which concerns on one Example of this invention. It is a graph of the relative expression level of collagen at the time of using the cosmetic composition and tension stimulus which concern on one Example of this invention. 1 is a cross-sectional view of a skin application device according to an embodiment of the present invention. It is a perspective view of an upper ball installation part (a) and a lower ball installation part (b) of a skin application device concerning one example of the present invention. It is a graph which shows the measurement result of a skin cooling experiment at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is a graph which shows the skin temperature reduction rate at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is the photograph which image | photographed the skin temperature of the eyelid at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is a graph which shows the measurement result of the wrinkle experiment of the eyelash at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is a graph which shows the wrinkle reduction rate of a crease when using the cosmetic composition and skin coating apparatus which concern on one Example of this invention. It is the photograph which image | photographed the wrinkle of the eyes in the case of using the cosmetic composition and skin coating apparatus which concern on one Example of this invention. It is a graph which shows the elasticity increase rate of the eyelash at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is a graph which shows the measurement result of the elasticity experiment of the eyelash at the time of using the cosmetics composition and skin coating apparatus which concern on one Example of this invention. It is the graph (a) which shows the skin blood flow rate at the time of using the cosmetic composition and skin coating apparatus which concern on one Example of this invention, and the image (b) of a blood flow rate. It is the graph (a) of the absorption amount of an active ingredient, and the graph (b) of an absorption rate at the time of using the cosmetic composition and skin coating device which concern on one Example of this invention.

  In the following, preferred embodiments of the present invention will be described in detail in order to enable a person having ordinary knowledge in the technical field to which the present invention belongs to easily carry out the present invention.

  The present invention provides a cosmetic cosmetic kit comprising a cosmetic composition containing arginine and lysine-containing polypeptides as active ingredients, and a skin application device for applying the cosmetic composition to the skin. The cosmetic composition itself has skin cooling, skin elasticity enhancement and skin wrinkle improvement effects.

  When the cosmetic composition described above is applied to the skin, it is applied while applying a tensile stimulus to the skin using a skin application device. The skin application device is not particularly limited, but may be one or more selected from the group consisting of a roller for applying a tensile stimulus, a shiatsu device, and a massage device.

  A tensile stimulus refers to a physical stimulus that applies pressure to the skin to cause it to expand and contract. Tensile stimuli strengthen the cytoskeletal proteins of skin cells. As can be seen in FIG. 1, actin in the cytoskeletal protein of fibroblasts is enhanced upon tensile stimulation.

  In addition, the tensile stimulus expresses a skin cell anti-aging and barrier function improving gene. Table 1 shows the related research results.

  In addition, tensile stimuli improve skin blood flow and promote skin absorption of the substance. And it is useful for immediate skin cooling effect, skin wrinkle, and elasticity improvement effect. About this, the effect is confirmed in the following experiment example.

  Since the tension stimulation to the skin using the skin application device generates a massage effect and an effect of promoting penetration of the active ingredient, the skin elasticity enhancing effect of the cosmetic composition containing the arginine and lysine-containing polypeptide as the active ingredient is effective. Help to be even better. Further, since the skin application device conducts heat of the skin and applies a tensile stimulus while directly contacting the skin, the skin cooling effect can be further improved. That is, the simultaneous use of the cosmetic composition and the skin application device produces a synergistic effect of skin cooling, skin elasticity enhancement, and skin wrinkle improvement effects.

  Preferably, the skin application device may be a roll-on. The roll-on may have one, two, three or more balls. Such a roll-on is not limited as long as a spherical ball is brought into contact with the skin.

  As an example, FIG. 3 shows a roll-on with three balls in such a skin application device. FIG. 3a is a cross-sectional view of the roll-on, which is a cross-sectional view through two of the three balls. FIG. 3b is a cross-sectional view through one of the three balls. FIG. 4 is a perspective view of an upper ball installation part (a) and a lower ball installation part (b) which are a part of the roll-on.

  Specifically, as seen in FIG. 3, the cosmetic composition is stored inside the lower container 10, the lower ball setting unit 30 supports the lower part of the ball 90, and the upper ball setting unit fixes the ball 90. The ball 90 is a spherical metal sphere, and is configured to rotate between the lower and upper ball mounting portions. When the cosmetic composition is applied to the surface of the ball on the container inner side, the ball rotates. The applied cosmetic composition is transmitted to the outside and applied to the skin. After the roll-on is used, the lid can be closed and stored, and the lid is one in which the lid outer cap 50 and the inner cap 60 are superimposed. FIG. 3 is merely an example of the present invention, and is not limited thereto.

  The cosmetic composition may further contain one or more of oat extract and yeast extract. At this time, the cosmetic composition contains 0.01 to 2% by weight of arginine and lysine-containing polypeptide, 2 to 6% by weight of oat extract, and 0.01 to 0% of yeast extract with respect to the total weight of the composition. 0.5% by weight, more preferably 0.01 to 1.5% by weight of arginine and lysine-containing polypeptide, 3 to 5% by weight of oat extract, and 0.06 to 0. 0% of yeast extract. It may be 2% by weight. When contained in a content within the above range, the effect of each active ingredient on the skin is exhibited and skin stability can be maintained.

  The cosmetic composition may further contain one or more selected from the group consisting of adenosine, retinol and niacinamide. Preferably, adenosine may be 0.01-0.07% by weight and retinol 50-150 IU based on the total weight of the composition.

  Arginine and lysine-containing polypeptides used in the present invention promote synthesis of extracellular components such as collagen I, collagen III, collagen V, decorin (Decorin, proteoglycane), and inhibit the release of MMP-1 And has an anti-aging effect. Representative examples of commercially available arginine and lysine-containing polypeptides include Solabia's PEPTISKIN (registered trademark) and the like. PEPTISKIN (registered trademark) is a 40% dry extraction solution composed of L-arginine and L-lysine oligopeptide, having a polymerization degree of 2 to 7, and obtained by a non-solvent enzymatic synthesis method.

  The oat extract used in the present invention is obtained by extracting from oat (Oat). Oats are cereals also called Avena sativa, and oats are rich in high-quality proteins, minerals such as phosphorus, magnesium and calcium, so they are not only suitable as diet foods, but also effective for constipation, It is mainly used for food. The oat extract used in the cosmetic composition of the present invention is extracted by an ordinary method for producing a cereal extract. As an example, the oats may be extracted into a pulverized product obtained by drying or pulverizing the oats, for example, with a solvent selected from the group consisting of distilled water, an organic solvent, and a mixture thereof. The organic solvent used at this time may be 1,3-butylene glycol, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, and two kinds of the organic solvents are sequentially used according to the polarity of the organic solvent. You may use it above. A typical example of the commercial oat extract is OSILIFT (registered trademark) manufactured by Silab. OSILIFT (registered trademark) is an Oat kernel extract, and is a substance excellent in an immediate skin lifting effect and wrinkle alleviation effect.

  The yeast extract used in the present invention is obtained by purifying and extracting yeast by microbial fermentation. In general, a yeast extract has a function of promoting the synthesis of collagen IV, collagen VII and integrin, and has TGF-β1 activity. Therefore, it has an anti-wrinkle effect and a dermis-epidermis junction strengthening effect. The yeast extract is not limited as long as it is a general extraction method, but may be prepared by the following method. After dissolving yeast (Saccharomyces cerevisiae) in water, separating the portion dissolved by filtration and the portion not dissolved, separately taking the dissolved portion and concentrating, then filtering and extracting the yeast To obtain a yeast extract. A typical example of a commercially available yeast extract is TONISKIN (registered trademark) (also called Yeast extract) manufactured by Silab.

  In the skin beauty kit, the cosmetic composition may be contained in the skin application device, and the cosmetic composition may be included separately without being contained in the skin application device.

  The present invention includes a step of applying a tensile stimulus to the skin using a skin application device at the same time as or after applying a cosmetic composition containing arginine and lysine-containing polypeptide as active ingredients to the skin. A method of applying the composition is provided.

  In the application method, the skin application device may be a roll-on. The roll-on may have one, two, three or more balls.

  Such roll-on is not limited as long as a spherical roll is brought into contact with the skin.

  The use of the skin application device using the cosmetic composition according to the present invention includes a yeast extract having a dermis-skin boundary strengthening effect and an oat extract having an immediate wrinkle mitigating effect through natural polyose. Synergistic effects of skin cooling and increased elasticity can be achieved by the cosmetic composition to be contained and a roll-on massage device having a massage effect by rolling and an effect of promoting penetration of active ingredients.

  In particular, when using a roll-on type skin application device, it is not necessary to use the hand, the cosmetic composition is not absorbed by the hand, and a large amount of the composition can be applied to the application site. There is an advantage that it is possible. Moreover, there is no restriction | limiting of the usability | use_conditions, such as a stickiness felt from a hand, and a feeling of after-remaining, You may use a foaming agent, a polymer, etc. in excess. In particular, when using a roll-on type skin application device, a tension effect is applied to the skin to give a massage effect, increase blood flow, promote the absorption of active ingredients, and improve skin cooling effect, wrinkle improvement effect and There is an advantage that comes to show the effect of elasticity improvement.

  Through the following implementation, the present invention will be described in more detail. However, the examples are for illustrating the present invention, and the scope of the present invention is not limited to these examples.

[Experimental Example 1] Collagen expression level measurement experiment Collagen relative expression level was measured with respect to skin fibroblasts. First, the skin fibroblasts were compared before and after applying a tensile stimulus. The tensile stimulus was 0.5 Hz and 17% tension was performed for 1 hour. In addition, PEPTISKIN (registered trademark) (manufactured by Solabia) was treated as a arginine and lysine-containing polypeptide on skin fibroblasts, and the results were compared before and after applying tensile stimulation under the same conditions as described above. The results are shown in FIG.

  As can be seen in FIG. 2, it was confirmed that the collagen expression level of the skin fibroblasts was increased only by tensile stimulation alone. In addition, when tensile stimulation was applied to skin fibroblasts treated with PEPTISKIN (registered trademark), the highest expression level of collagen was observed. That is, when a tensile stimulus and PEPTISKIN (registered trademark) were given at the same time, a synergy effect was exhibited and the most excellent effect was exhibited.

[Example 1]
As the oat extract, an oat extract under the trade name OSILIFT (registered trademark) (manufactured by Silab) was used. As the arginine and lysine-containing polypeptide, a polypeptide having a trade name of PEPTISKIN (registered trademark) (manufactured by Solabia) was used. As the yeast extract, a yeast extract under the trade name TONISKIN (registered trademark) (manufactured by Silab) was used. OSILIFT® 4% by weight, PEPTISKIN® 0.01% by weight, TONISKIN® 0.5% by weight, adenosine 0.04% by weight and retinol 100 IU were mixed. Substances were added at the ratios shown in Table 2 to produce essences.

  The essence thus produced was injected into a roll-on applicator that is a skin application device. The roll-on applicator used is as shown in FIG.

[Experimental Example 2] Skin Cooling Experiment Using the essence produced in Example 1, a clinical test was conducted on 10 subjects. The age of 10 subjects was 32 ± 3.2 years. The test was performed with the right eye of each subject as the test group and the left eye as the control group.

  The subject was adapted for 15 minutes in a constant temperature and humidity room at 22 to 28 ° C. and a relative humidity of 108 to 42% after washing the face. The test group of subjects massaged with a roll-on applicator which is a skin application device according to the present invention for 30 seconds, applied the essence of Example 1, and the control group absorbed the same amount of the same emulsion by hand. It was. Thereafter, the face was photographed before and after the essence application.

  A skin temperature measurement camera (IR camera, CAM PM575, FLIR. USA) was used to examine immediate changes in skin temperature. The results are shown in FIGS.

  As can be seen from FIG. 5 and FIG. 6, it was found that the skin temperature of the eyes decreased before and immediately after the use of the roll-on applicator, and the skin temperature reduction rate was much larger than that of the control group. FIG. 7 shows a photograph of the skin temperature distribution around the eyes of two subjects photographed with a camera. Also from FIG. 7, it can be confirmed that the difference in skin temperature decrease is more certain in the test group, demonstrating that the present invention is excellent in immediate cooling effect.

[Experimental Example 3] Skin Wrinkle Experiment A skin wrinkle experiment was performed using the essence produced in Example 1 above, with the subject, test group and control group being the same as in Experimental Example 2.

  Measurement was performed before, after, and after application of the essence of Example 1, and after 2 weeks, 4 weeks and 8 weeks.

  When photographing a face, a wrinkle measuring instrument (primos, GFMESSTECHNIK. Germany) was used to photograph a wrinkle on the eyes, and a change in the wrinkles on the eyes was examined. The measurement results are shown in FIGS. 8 and 9, and the photographic images are shown in FIG. Table 3 shows a significant difference with respect to pre-use as a result of paired t-test, and Table 4 shows a significant difference between groups as a result of independent t-test.

  As can be seen from FIG. 8 and FIG. 9, the wrinkles on the eyes decreased by 11.7% after 8 weeks of use of the test group products. On the other hand, the wrinkle reduction effect did not appear until 8 weeks in the case of the control group product. Further, as can be seen from FIG. 10, after 8 weeks of use with respect to 0 weeks, it was possible to visually observe the reduction in wrinkles. In addition, as seen from Tables 2 and 3, 8 weeks after the use of the product, the wrinkle improvement effect between the test group and the control group was compared with the pre-use comparison and the significant difference between groups (significant difference * p <0.05). ) Was confirmed. This seems to be because the absorption of the active ingredient was promoted by rolling the roll-on applicator.

[Experimental Example 4] Skin Elasticity Experiment The skin elasticity experiment was performed using the essence produced in Example 1, with the subject, test group, and control group being the same as in Experimental Example 2.

  Measurement was performed before, after, and after application of the essence of Example 1, and after 2 weeks, 4 weeks and 8 weeks.

  At the time of photographing a face, a skin elasticity measuring device (Cutometer 580 (registered trademark), C + K, Germany) was used to examine changes in the elasticity of the skin around the eyes. The results are shown in FIG. 11 and FIG.

  As can be seen from FIGS. 11 and 12, after 2 weeks, 4 weeks and 8 weeks of use of the test group products, the elasticity of the eyes is 0.4%, 6.6% and 9.1% (p = 0.0.1), respectively. 062) It was found that there is a tendency to increase. On the other hand, in the case of the control group product, only 2.7% up to 8 weeks showed only a tendency to increase the elasticity of eyes. This seems to be due to the massage effect of the eyes by the roll-on applicator.

[Experimental Example 5] Blood flow measurement experiment Using the essence produced in Example 1, the subjects, the test group, and the control group were the same as in Experimental Example 2, and the blood flow rate of the skin was measured. The results are shown in FIG. 13 (a) blood flow change comparison graph by application method, b) blood flow image).

  As can be seen from FIG. 13a, it was observed that the blood flow increased by about 40% after use than before the use of the roll-on applicator of the present invention, and the control group using the hand rather decreased the blood flow. Also, as can be seen from FIG. 13b, the value of the Perfusion color scale increases, and the closer to red, the faster the blood flow speed. The lower the value, the closer to the cold blue color, the more blood flow It was confirmed that the blood flow rate was faster than before application. Thereby, it confirmed that blood flow increased by the massage effect by the roll-on applicator of this invention.

[Experimental Example 6] Experiment of skin absorption promoting effect Using the essence produced in Example 1, the subject, test group and control group were measured in the same manner as in Experimental Example 2, and the degree of skin absorption of the active ingredient was measured. . The results are shown in FIG. 14 (a) an absorption comparison graph of active ingredients by the coating method, and b) absorption rate comparison graph).

  As can be seen from FIG. 14a, when using the roll-on applicator of the present invention, the amount of absorption of the active ingredient is increased by about 40% compared to the case of manual application, and as can be seen from FIG. Increased 2 times. From this, when using the roll-on applicator of the present invention, it was confirmed that the amount of the active ingredient transmitted through the skin was increased by the physical stimulation of the roll-on, and the initial absorption rate was increased by the massage effect of the roll-on.

DESCRIPTION OF SYMBOLS 10 Lower container 20 Upper container 30 Upper ball installation part 40 Lower ball installation part 50 Cap outside lid 60 Cap inside lid 70 Cap decoration 80 Liner 90 Ball

Claims (4)

A cosmetic kit for skin cooling comprising a cosmetic composition containing arginine and lysine-containing polypeptide as active ingredients, and a skin application device for applying the cosmetic composition to the skin,
The cosmetic composition is
Arginine and lysine-containing polypeptide 0.01 to 2% by mass, oat extract 2 to 6% by mass, and yeast extract 0.01 to 0.5% by mass with respect to the total weight of the composition,
Containing 0.01 to 0.07% by weight of adenosine and 50 to 150 IU of retinol, based on the total weight of the composition;
The skin application device comprises:
It is a roll-on type having three or more metal balls that give a tensile stimulus,
Used to lower skin temperature,
Beauty kit for skin cooling. The skin cooling beauty kit according to claim 1, wherein the skin beauty kit also has one or more selected from the group consisting of a skin elasticity enhancement use and a skin wrinkle improvement use. Application of a cosmetic composition comprising a step of applying a tensile stimulus to the skin using a skin application device at the same time as or after applying a cosmetic composition containing an arginine and lysine-containing polypeptide as an active ingredient A method,
The cosmetic composition is
Arginine and lysine-containing polypeptide 0.01 to 2% by mass, oat extract 2 to 6% by mass, and yeast extract 0.01 to 0.5% by mass with respect to the total weight of the composition,
Containing 0.01 to 0.07% by weight of adenosine and 50 to 150 IU of retinol, based on the total weight of the composition;
The skin application device comprises:
It is a roll-on type having three or more metal balls that give a tensile stimulus,
Used to lower skin temperature,
Application method. The said application | coating method is a coating method of the cosmetic composition of Claim 3 which has one or more selected from the group which consists of a skin cooling use, a skin elasticity promotion use, and a skin wrinkle improvement use.