JP6148183B2 - セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法 - Google Patents

セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法 Download PDF

Info

Publication number
JP6148183B2
JP6148183B2 JP2013558217A JP2013558217A JP6148183B2 JP 6148183 B2 JP6148183 B2 JP 6148183B2 JP 2013558217 A JP2013558217 A JP 2013558217A JP 2013558217 A JP2013558217 A JP 2013558217A JP 6148183 B2 JP6148183 B2 JP 6148183B2
Authority
JP
Japan
Prior art keywords
sequence
glucosidase
seq
polypeptide
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2013558217A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014509858A (ja
JP2014509858A5 (https=
Inventor
ケイパー、シジス
ニコラエフ、イゴール
ランツ、スザンヌ
フジダラ、メレディス・ケー
シー、ミーガン・ワイ
Original Assignee
ダニスコ・ユーエス・インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク filed Critical ダニスコ・ユーエス・インク
Publication of JP2014509858A publication Critical patent/JP2014509858A/ja
Publication of JP2014509858A5 publication Critical patent/JP2014509858A5/ja
Application granted granted Critical
Publication of JP6148183B2 publication Critical patent/JP6148183B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445Beta-glucosidase (3.2.1.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021Beta-glucosidase (3.2.1.21)
    • DTEXTILES; PAPER
    • D06TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06MTREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Textile Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2013558217A 2011-03-17 2012-03-16 セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法 Expired - Fee Related JP6148183B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161453918P 2011-03-17 2011-03-17
US61/453,918 2011-03-17
PCT/US2012/029498 WO2012125951A1 (en) 2011-03-17 2012-03-16 Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars

Publications (3)

Publication Number Publication Date
JP2014509858A JP2014509858A (ja) 2014-04-24
JP2014509858A5 JP2014509858A5 (https=) 2015-04-30
JP6148183B2 true JP6148183B2 (ja) 2017-06-21

Family

ID=45888505

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013558217A Expired - Fee Related JP6148183B2 (ja) 2011-03-17 2012-03-16 セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法

Country Status (13)

Country Link
US (2) US20140073017A1 (https=)
EP (1) EP2686427A1 (https=)
JP (1) JP6148183B2 (https=)
KR (1) KR20140023313A (https=)
CN (2) CN103492561A (https=)
AU (1) AU2012228968B2 (https=)
BR (1) BR112013023715A2 (https=)
CA (1) CA2829918A1 (https=)
MX (1) MX2013010509A (https=)
RU (1) RU2013146341A (https=)
SG (1) SG192097A1 (https=)
WO (1) WO2012125951A1 (https=)
ZA (1) ZA201305532B (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
US8980050B2 (en) 2012-08-20 2015-03-17 Celanese International Corporation Methods for removing hemicellulose
JP6290626B2 (ja) 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
CA2888753A1 (en) * 2012-10-31 2014-05-08 Danisco Us Inc. Beta-glucosidase from magnaporthe grisea
WO2014088940A1 (en) 2012-12-07 2014-06-12 Danisco Us Inc. Compositions and methods of use
US10059932B2 (en) * 2013-03-15 2018-08-28 Nagaoka University Of Technology Mutant of cellulase-producing microorganism, production method of cellulase and production method of cello-oligosaccharide
MX2016001317A (es) 2013-07-29 2016-04-07 Danisco Us Inc Variantes de enzimas.
FR3014903B1 (fr) * 2013-12-17 2017-12-01 Ifp Energies Now Procede d'hydrolyse enzymatique avec production in situ de glycosides hydrolases par des microorganismes genetiquement modifies (mgm) et non mgm
TW201527756A (zh) * 2014-01-10 2015-07-16 Nat Univ Tsing Hua 提供食品安全地圖之方法、電腦程式產品、和系統
JP6465470B2 (ja) * 2014-01-22 2019-02-06 本田技研工業株式会社 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法
JP6398115B2 (ja) * 2014-01-22 2018-10-03 本田技研工業株式会社 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法
JP6398116B2 (ja) * 2014-01-22 2018-10-03 本田技研工業株式会社 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法
JP2017524363A (ja) 2014-08-08 2017-08-31 ザイレコ,インコーポレイテッド アグリコシル化された酵素およびその使用
US20180148683A1 (en) 2015-07-07 2018-05-31 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
DK3419992T3 (da) 2016-02-22 2021-02-15 Danisco Us Inc Svampesystem til proteinproduktion med højt udbytte
EP3693449A1 (en) * 2016-04-29 2020-08-12 Novozymes A/S Detergent compositions and uses thereof
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
EP3523415A1 (en) 2016-10-04 2019-08-14 Danisco US Inc. Protein production in filamentous fungal cells in the absence of inducing substrates
MX2019007335A (es) 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
EP3375884B1 (en) * 2017-03-15 2020-05-06 Clariant International Ltd Process for producing proteins under inducing conditions
WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE
US12416026B2 (en) 2019-08-29 2025-09-16 Danisco Us Inc. Expression of beta-glucosidase in yeast for improved ethanol production
CN115125152A (zh) * 2022-03-28 2022-09-30 湖南科技学院 一种降解木质纤维素的混合菌、混合酶和降解方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366558A (en) 1979-03-23 1994-11-22 Brink David L Method of treating biomass material
DK494089D0 (https=) * 1989-10-06 1989-10-06 Novo Nordisk As
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
US5405769A (en) 1993-04-08 1995-04-11 National Research Council Of Canada Construction of thermostable mutants of a low molecular mass xylanase
US5705369A (en) 1994-12-27 1998-01-06 Midwest Research Institute Prehydrolysis of lignocellulose
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
ES2237159T3 (es) 1998-10-06 2005-07-16 Mark Aaron Emalfarb Sistema de transformacion en el campo de los hongos micoticos filamentosos en chrysosporium.
US6409841B1 (en) 1999-11-02 2002-06-25 Waste Energy Integrated Systems, Llc. Process for the production of organic products from diverse biomass sources
US6423145B1 (en) 2000-08-09 2002-07-23 Midwest Research Institute Dilute acid/metal salt hydrolysis of lignocellulosics
JP2004527261A (ja) 2001-05-18 2004-09-09 ノボザイムス アクティーゼルスカブ セロビアーゼ活性を有するポリペプチド及びそれをコードするポリヌクレオチド
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
KR101148255B1 (ko) 2002-10-04 2012-08-08 다니스코 유에스 인크. 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법
AU2003291395A1 (en) 2002-11-07 2004-06-03 Genencor International, Inc. Bgl6 beta-glucosidase and nucleic acids encoding the same
AU2003219956A1 (en) 2003-02-27 2004-09-28 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
CN101374948B (zh) 2003-04-01 2012-04-11 金克克国际有限公司 灰腐质霉cbh1.1变体
ATE524491T1 (de) 2003-05-29 2011-09-15 Genencor Int Neue trichoderma-gene
AU2005249422B2 (en) 2004-05-27 2011-03-17 Genencor International, Inc. Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
US7781191B2 (en) 2005-04-12 2010-08-24 E. I. Du Pont De Nemours And Company Treatment of biomass to obtain a target chemical
EP1869194A2 (en) 2005-04-12 2007-12-26 E.I.Du pont de nemours and company Treatment of biomass to obtain a target chemical
WO2007094852A2 (en) * 2006-02-10 2007-08-23 Verenium Corporation Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
US9512448B2 (en) * 2007-05-09 2016-12-06 Stellenbosch University Method for enhancing cellobiose utilization
DK2152892T3 (da) 2007-05-21 2013-07-22 Danisco Us Inc Genencor Div Fremgangsmåde til indføring af nukleinsyrer i fungale celler
MX2009012844A (es) * 2007-05-31 2009-12-15 Novozymes Inc Composiciones para degradar material celulosico.
KR20100028031A (ko) * 2007-06-06 2010-03-11 다니스코 유에스 인크. 단백질 특성의 개선 방법
CN101796195A (zh) * 2007-09-07 2010-08-04 丹尼斯科美国公司 β-葡糖苷酶增强的丝状真菌全纤维素酶组合物和使用方法
CN101952421A (zh) * 2007-12-05 2011-01-19 诺维信公司 具有内切葡聚糖酶活性的多肽和编码所述多肽的多核苷酸
CN105368764B (zh) 2007-12-13 2019-03-12 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
US10676751B2 (en) * 2008-02-29 2020-06-09 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
US8772010B2 (en) * 2009-06-16 2014-07-08 Codexis, Inc. β-Glucosidase variants
JP5641478B2 (ja) * 2010-07-18 2014-12-17 独立行政法人国際農林水産業研究センター 酵素の再利用方法
DK2748317T3 (en) * 2011-08-22 2017-07-17 Codexis Inc GH61 glycoside hydrolase protein variants and cofactors that enhance GH61 activity
BR112014007651A2 (pt) * 2011-09-30 2017-04-11 Novozymes Inc polipeptídeo quimérico isolado, polinucleotídeo isolado, métodos para produzir um polipeptídeo quimérico e um produto de fermentação, para degradar ou converter um material celulósico, e para fermentar um material celulósico, planta, parte de planta ou célula de planta transgênica, e, formulação de caldo inteiro ou composição de cultura de células
EP2914719A1 (en) * 2012-10-31 2015-09-09 Danisco US Inc. Compositions and methods of use

Also Published As

Publication number Publication date
AU2012228968B2 (en) 2017-05-18
JP2014509858A (ja) 2014-04-24
MX2013010509A (es) 2013-10-17
BR112013023715A2 (pt) 2016-09-13
ZA201305532B (en) 2014-10-29
US20180119125A1 (en) 2018-05-03
CN109371002A (zh) 2019-02-22
RU2013146341A (ru) 2015-04-27
KR20140023313A (ko) 2014-02-26
EP2686427A1 (en) 2014-01-22
CN103492561A (zh) 2014-01-01
US20140073017A1 (en) 2014-03-13
WO2012125951A1 (en) 2012-09-20
SG192097A1 (en) 2013-08-30
CA2829918A1 (en) 2012-09-20

Similar Documents

Publication Publication Date Title
JP6148183B2 (ja) セルラーゼ組成物並びにこれを用いリグノセルロース系バイオマスの発酵性糖質への変換を向上させる方法
JP6290626B2 (ja) 糖化プロセスにおける粘度を低下させる方法
AU2012229042B2 (en) Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
JP5932648B2 (ja) 新規なグリコシルヒドロラーゼ酵素及びその用途
AU2012228968A1 (en) Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
AU2012229042A1 (en) Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
AU2016203478A1 (en) Method for reducing viscosity in saccharification process
AU2012229030A1 (en) Method for reducing viscosity in saccharification process

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150311

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20150311

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20160129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160202

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160427

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160630

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161004

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161208

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170223

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170403

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170418

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170518

R150 Certificate of patent or registration of utility model

Ref document number: 6148183

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees