JP6113736B2 - 調節可能なプロモーター - Google Patents

調節可能なプロモーター Download PDF

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Publication number
JP6113736B2
JP6113736B2 JP2014533924A JP2014533924A JP6113736B2 JP 6113736 B2 JP6113736 B2 JP 6113736B2 JP 2014533924 A JP2014533924 A JP 2014533924A JP 2014533924 A JP2014533924 A JP 2014533924A JP 6113736 B2 JP6113736 B2 JP 6113736B2
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seq
promoter
poi
carbon source
cell
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Japanese (ja)
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JP2014530016A (ja
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マッタノビッチ、ディエサルド
ガゼル、ブリジッテ
モーレル、ミヒャエル
プリエルホファー、ローランド
ケレイン、ヨアヒム
ベンガー、ヤナ
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Lonza AG
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Lonza AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2014533924A 2011-10-07 2012-10-05 調節可能なプロモーター Active JP6113736B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161544451P 2011-10-07 2011-10-07
EP11184323 2011-10-07
US61/544,451 2011-10-07
EP11184323.1 2011-10-07
EP12171006.5 2012-06-06
EP12171006 2012-06-06
PCT/EP2012/069757 WO2013050551A1 (en) 2011-10-07 2012-10-05 Regulatable promoter

Publications (2)

Publication Number Publication Date
JP2014530016A JP2014530016A (ja) 2014-11-17
JP6113736B2 true JP6113736B2 (ja) 2017-04-12

Family

ID=48043194

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014533924A Active JP6113736B2 (ja) 2011-10-07 2012-10-05 調節可能なプロモーター

Country Status (17)

Country Link
US (4) US9512432B2 (da)
EP (2) EP2764016B1 (da)
JP (1) JP6113736B2 (da)
KR (2) KR102128957B1 (da)
CN (3) CN113788884A (da)
AR (1) AR088252A1 (da)
AU (1) AU2012320421B2 (da)
BR (1) BR112014008152A2 (da)
CA (1) CA2849856C (da)
DK (1) DK3508494T3 (da)
ES (1) ES2913825T3 (da)
IL (1) IL231859B (da)
IN (1) IN2014DN03419A (da)
PL (1) PL2764016T3 (da)
SG (2) SG11201400811QA (da)
TW (1) TWI560272B (da)
WO (1) WO2013050551A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102128957B1 (ko) * 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
CN104195165A (zh) * 2014-09-01 2014-12-10 北京爱必信生物技术有限公司 包含葡萄糖启动子的载体、宿主菌以及重组牛源胰蛋白酶的制备方法
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
ES2902044T3 (es) * 2015-08-05 2022-03-24 Lonza Ag Variantes de promotor
US11377677B2 (en) 2016-03-02 2022-07-05 Lonza Ltd Fermentation process
EP3556856A4 (en) * 2016-12-15 2020-06-24 Kaneka Corporation NOVEL HOST CELL AND TARGET PROTEIN PRODUCTION METHOD USING THE SAME
CN112105737A (zh) * 2018-02-12 2020-12-18 龙沙有限公司 生产目标蛋白质的宿主细胞
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
US11773424B2 (en) 2019-01-11 2023-10-03 Lonza Ltd Carbon-source regulated protein production in a recombinant host cell
ES2948239T3 (es) * 2019-01-29 2023-09-06 Clariant Produkte Deutschland Gmbh Promotor quimérico para su uso en ingeniería metabólica
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
CA3135444A1 (en) 2019-04-01 2020-10-08 Domen ZAVEC Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
MX2022000374A (es) 2019-07-11 2022-03-25 Clara Foods Co Composiciones de proteina y productos consumibles de las mismas.
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
AU2021240417A1 (en) 2020-03-26 2022-10-20 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
US20230126246A1 (en) 2020-04-01 2023-04-27 Lonza Ltd Helper factors for expressing proteins in yeast
EP4136237A1 (en) 2020-04-15 2023-02-22 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
EP4222266A1 (en) 2020-09-30 2023-08-09 Lonza Ltd. Host cells overexpressing translational factors
CN117120618A (zh) 2021-02-12 2023-11-24 勃林格殷格翰 Rcv 两合公司 用于增加蛋白质分泌的信号肽

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU577259B2 (en) 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
US4808537A (en) 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
EP0922109A1 (en) 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
SE9802333D0 (sv) 1998-06-29 1998-06-29 Astra Pharma Prod Novel combination
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
CA2589591A1 (en) 2004-07-22 2006-03-09 Glycofi, Inc. Malate synthase regulatory sequences for heterologous gene expression in pichia
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
EP1951877B1 (en) 2005-11-16 2010-11-03 Korea Research Institute of Bioscience and Biotechnology Translational elongation factor promoter from pichia pastoris and method for producing recombinant protein using the same
KR100717353B1 (ko) 2006-04-12 2007-05-11 한국생명공학연구원 피키아 파스토리스 유래의 자동 유도성 nps 프로모터 및이를 이용한 이종 단백질의 제조방법
CN100552029C (zh) 2006-09-14 2009-10-21 南开大学 毕赤酵母ω3-脂肪酸脱氢酶启动子序列及其应用
US8222386B2 (en) 2006-10-10 2012-07-17 Keck Graduate Institute P. pastoris ADH promoter and use thereof to direct expression of proteins
AU2008241061A1 (en) 2007-04-20 2008-10-30 Polymun Scientific Immunbiologische Forschung Gmbh Yeast expression systems
US8105811B2 (en) 2008-06-13 2012-01-31 Wisconsin Alumni Research Foundation Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
WO2010135678A1 (en) 2009-05-22 2010-11-25 Research Corporation Technologies, Inc. Nucleic acids of pichia pastoris and use thereof for recombinant production of proteins
US20120184020A1 (en) * 2009-07-09 2012-07-19 Verdezyne, Inc. Engineered microorganisms with enhanced fermentation activity
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process
KR102128957B1 (ko) * 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
ES2902044T3 (es) 2015-08-05 2022-03-24 Lonza Ag Variantes de promotor

Also Published As

Publication number Publication date
AU2012320421A1 (en) 2014-03-20
US11976284B2 (en) 2024-05-07
EP2764016B1 (en) 2019-03-13
CN103998460A (zh) 2014-08-20
AR088252A1 (es) 2014-05-21
SG11201400811QA (en) 2014-08-28
US11401523B2 (en) 2022-08-02
US20140242636A1 (en) 2014-08-28
KR102128957B1 (ko) 2020-07-02
KR20140069112A (ko) 2014-06-09
BR112014008152A2 (pt) 2017-04-18
CN110055248A (zh) 2019-07-26
US20220259608A1 (en) 2022-08-18
TW201317350A (zh) 2013-05-01
US10301634B2 (en) 2019-05-28
EP2764016A1 (en) 2014-08-13
EP3508494A1 (en) 2019-07-10
US20190185866A1 (en) 2019-06-20
CA2849856C (en) 2023-03-14
US9512432B2 (en) 2016-12-06
KR101954389B1 (ko) 2019-03-06
ES2913825T3 (es) 2022-06-06
AU2012320421B2 (en) 2017-03-02
CA2849856A1 (en) 2013-04-11
DK3508494T3 (da) 2022-05-23
IL231859A0 (en) 2014-05-28
KR20190025048A (ko) 2019-03-08
US20170037418A1 (en) 2017-02-09
JP2014530016A (ja) 2014-11-17
TWI560272B (en) 2016-12-01
WO2013050551A1 (en) 2013-04-11
IN2014DN03419A (da) 2015-05-22
EP3508494B1 (en) 2022-02-23
CN113788884A (zh) 2021-12-14
IL231859B (en) 2020-08-31
PL2764016T3 (pl) 2019-08-30
SG10201602673PA (en) 2016-05-30

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