JP6090855B2 - 心筋細胞および／または心筋前駆細胞増殖剤ならびに心筋細胞および／または心筋前駆細胞の増殖方法 - Google Patents

心筋細胞および／または心筋前駆細胞増殖剤ならびに心筋細胞および／または心筋前駆細胞の増殖方法 Download PDF

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JP6090855B2
JP6090855B2 JP2013523110A JP2013523110A JP6090855B2 JP 6090855 B2 JP6090855 B2 JP 6090855B2 JP 2013523110 A JP2013523110 A JP 2013523110A JP 2013523110 A JP2013523110 A JP 2013523110A JP 6090855 B2 JP6090855 B2 JP 6090855B2
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cells
inhibitor
cardiomyocyte
myocardial progenitor
myocardial
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山下　潤
潤 山下
英毅 魚崎
英毅 魚崎
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
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  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013523110A 2010-11-17 2011-11-17 心筋細胞および／または心筋前駆細胞増殖剤ならびに心筋細胞および／または心筋前駆細胞の増殖方法 Expired - Fee Related JP6090855B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41481210P 2010-11-17 2010-11-17
US61/414,812 2010-11-17
PCT/JP2011/077266 WO2012067266A1 (en) 2010-11-17 2011-11-17 Cardiomyocyte- and/or cardiac progenitor cell-proliferating agent and method for proliferating cardiomyocytes and/or cardiac progenitor cells

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JP2013544071A JP2013544071A (ja) 2013-12-12
JP2013544071A5 JP2013544071A5 (enExample) 2015-01-08
JP6090855B2 true JP6090855B2 (ja) 2017-03-08

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JP2013523110A Expired - Fee Related JP6090855B2 (ja) 2010-11-17 2011-11-17 心筋細胞および／または心筋前駆細胞増殖剤ならびに心筋細胞および／または心筋前駆細胞の増殖方法

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US (1) US9074188B2 (enExample)
EP (1) EP2640829A4 (enExample)
JP (1) JP6090855B2 (enExample)
WO (1) WO2012067266A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011503232A (ja) 2007-11-20 2011-01-27 ザ ブリガム アンド ウィメンズ ホスピタル インコーポレイテッド 免疫応答の調節
US9164093B2 (en) * 2011-03-31 2015-10-20 Iheart Japan Corporation Cardiomyocyte marker
WO2014025315A1 (en) * 2012-08-07 2014-02-13 Singapore Health Services Pte Ltd Method, combination and/or composition for inducing cardiomyocyte differentation
EP2914298B1 (en) * 2012-11-02 2020-01-08 Duke University Inhibition of histone methyltransferase for cardiac reprogramming
WO2015119575A2 (en) * 2014-02-06 2015-08-13 Singapore Health Services Pte Ltd Improved method, combination and/or composition for inducing cardiomyocyte or neuronal differentation
EP3102207B1 (en) 2014-02-07 2022-05-11 Agency For Science, Technology And Research 2,4,5-tri-substituted azole-based casein kinase 1 inhibitors as inducers for cardiomyogenesis
WO2016154362A1 (en) 2015-03-23 2016-09-29 The Brigham And Women's Hospital, Inc. Tolerogenic nanoparticles for treating diabetes mellitus
WO2017181166A1 (en) * 2016-04-15 2017-10-19 Temple University-Of The Commonwealth System Of Higher Education Microrna-294 and ln28a as a driver of cardiac tissue proliferation in response to pathological injury
WO2019006512A1 (en) * 2017-07-07 2019-01-10 The University Of Queensland REGENERATION OF CARDIOMYOCYTES
CN111868078A (zh) * 2018-03-16 2020-10-30 斯坦福大学托管董事会 使用wnt激动剂和生物活性脂质用于生成和扩增心肌细胞的试剂和方法
WO2020037076A1 (en) 2018-08-14 2020-02-20 Emory University Inducing proliferation of cardiomyocytes and therapeutic uses related thereto
AU2019435745B2 (en) * 2019-03-18 2026-01-22 The Council Of The Queensland Institute Of Medical Research Cardiomyocyte proliferation
EP4003515B1 (en) * 2019-07-30 2025-10-22 Victor Chang Cardiac Research Institute Klf induced cardiomyogenesis
WO2022006094A1 (en) * 2020-06-30 2022-01-06 The Board Of Trustees Of The Leland Stanford Junior University MOLECULES REGULATING HUMAN iPSC-DERIVED CARDIOMYOCYTES PROLIFERATION BY INHIBITING CELL-CELL CONTACT
CN112481200A (zh) * 2020-12-25 2021-03-12 扬州大学 一种提升大鼠骨髓间充质干细胞分化为心肌细胞效率的方法
CN120041570B (zh) * 2025-02-28 2026-02-10 太原理工大学 CaMKII-α抑制剂在制备治疗T细胞淋巴瘤的药物中的应用

Family Cites Families (10)

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US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US6417185B1 (en) 1998-06-19 2002-07-09 Chiron Corporation Inhibitors of glycogen synthase kinase 3
US20020103360A1 (en) 1998-09-01 2002-08-01 Yang Pan Novel protein related to melanoma-inhibiting protein and uses thereof
ATE532863T1 (de) 2003-11-21 2011-11-15 Daiichi Sankyo Co Ltd Verfahren zur anzucht von myokardzellen
WO2006118914A2 (en) * 2005-04-29 2006-11-09 Children's Medical Center Corporation Methods of increasing proliferation of adult mammalian cardiomyocytes through p38 map kinase inhibition
CN101228264B (zh) 2005-06-22 2017-12-26 阿斯特利亚斯生物治疗股份公司 使灵长类多能干细胞分化成心肌细胞系细胞
CN101864392B (zh) 2005-12-13 2016-03-23 国立大学法人京都大学 核重新编程因子
GB0615327D0 (en) * 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
US8765465B2 (en) 2008-03-26 2014-07-01 Kyoto University Efficient production and use of highly cardiogenic progenitors and cardiomyocytes from embryonic and induced pluripotent stem cells
JP2010158206A (ja) 2009-01-08 2010-07-22 National Institute Of Advanced Industrial Science & Technology ヒト心筋前駆細胞の選別方法

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US9074188B2 (en) 2015-07-07
WO2012067266A1 (en) 2012-05-24
EP2640829A1 (en) 2013-09-25
US20130244262A1 (en) 2013-09-19
EP2640829A4 (en) 2014-06-11
JP2013544071A (ja) 2013-12-12

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