JP6083877B2 - 多能性幹細胞から骨格筋細胞への分化誘導方法 - Google Patents

多能性幹細胞から骨格筋細胞への分化誘導方法 Download PDF

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JP6083877B2
JP6083877B2 JP2014521869A JP2014521869A JP6083877B2 JP 6083877 B2 JP6083877 B2 JP 6083877B2 JP 2014521869 A JP2014521869 A JP 2014521869A JP 2014521869 A JP2014521869 A JP 2014521869A JP 6083877 B2 JP6083877 B2 JP 6083877B2
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myod
cell
skeletal muscle
expression
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JP2014533491A (ja
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英俊 櫻井
英俊 櫻井
田中　章仁
章仁 田中
ウォルツェン、クヌート
真 池谷
真 池谷
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Kyoto University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014521869A 2011-11-18 2012-08-17 多能性幹細胞から骨格筋細胞への分化誘導方法 Active JP6083877B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161561586P 2011-11-18 2011-11-18
US61/561,586 2011-11-18
PCT/JP2012/071373 WO2013073246A1 (en) 2011-11-18 2012-08-17 Method of inducing differentiation from pluripotent stem cells to skeletal muscle cells

Publications (3)

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JP2014533491A JP2014533491A (ja) 2014-12-15
JP2014533491A5 JP2014533491A5 (https=) 2015-10-08
JP6083877B2 true JP6083877B2 (ja) 2017-02-22

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US (1) US9862929B2 (https=)
JP (1) JP6083877B2 (https=)
WO (1) WO2013073246A1 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6566520B2 (ja) * 2013-10-29 2019-08-28 国立大学法人 熊本大学 筋疾患の治療または予防のための医薬組成物
ES2782380T5 (es) * 2013-10-30 2023-05-08 Univ Missouri Método de especificación y cultivo de linaje del músculo esquelético escalable
JP6845500B2 (ja) * 2014-12-29 2021-03-17 国立大学法人京都大学 骨格筋前駆細胞の製造方法
US10620109B2 (en) 2015-01-14 2020-04-14 Kyoto University Method for selecting skeletal muscle progenitor cell
KR102684690B1 (ko) 2015-03-03 2024-07-15 리젠츠 오브 더 유니버시티 오브 미네소타 Etv2 및 그의 용도
WO2017004388A1 (en) 2015-06-30 2017-01-05 Regents Of The University Of Minnesota Humanized heart muscle
RU2018103093A (ru) * 2015-06-30 2019-07-31 Реджентс Оф Зэ Юниверсити Оф Миннесота Гуманизированная скелетная мышца
SG10201912931RA (en) * 2015-08-31 2020-02-27 I Peace Inc Pluripotent stem cell manufacturing system, method for inducing stem cells, floating culture method for stem cells, floating culture vessel for stem cells, method for producing induced pluripotent stem cells, and method for producing particular somatic cells from animal cells
US11396644B2 (en) * 2016-01-31 2022-07-26 Keio University Method for promoting differentiation of pluripotent stem cells by reducing undifferentiated state thereof
US20190161731A1 (en) * 2016-04-06 2019-05-30 The General Hospital Corporation Direct reprogramming of somatic cells into myogenic cells
WO2017188458A1 (ja) * 2016-04-27 2017-11-02 武田薬品工業株式会社 骨格筋前駆細胞及び骨格筋細胞の製造方法
AU2017351638A1 (en) 2016-10-26 2019-06-13 Genea Biocells USA (Holdings), Inc. Improved generation of muscle lineage cells and therapeutic uses thereof
US11136555B2 (en) * 2017-03-01 2021-10-05 Elixirgen Scientific, Inc. Compositions and methods for differentiation of human pluripotent stem cells into desired cell types
WO2018208628A1 (en) 2017-05-06 2018-11-15 Memphis Meats, Inc. Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
WO2019014652A1 (en) 2017-07-13 2019-01-17 Memphis Meats, Inc. COMPOSITIONS AND METHODS FOR INCREASING THE EFFICACY OF CELL CULTURES USED IN FOOD PRODUCTION
JP7548494B2 (ja) * 2018-10-30 2024-09-10 国立大学法人京都大学 細胞の製造方法
WO2020204149A1 (ja) 2019-03-29 2020-10-08 公立大学法人横浜市立大学 スクリーニング方法および毒性評価法
US20240018479A1 (en) * 2020-10-26 2024-01-18 University Of Massachusetts Methods and compositions for treatment of muscle disease with ipsc-induced human skeletal muscle stem cells
CN112430624B (zh) * 2020-11-25 2023-08-18 汕头大学 一种斑马鱼肌肉特异诱导型表达载体及应用
US20240002806A1 (en) * 2022-03-10 2024-01-04 Innocent Meat GmbH Method for differentiating adult stem cells into final tissue
WO2023240152A1 (en) 2022-06-07 2023-12-14 Upside Foods, Inc. Engineering cell lines capable of proliferation in growth factor free media formulations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
US6201168B1 (en) * 1999-08-20 2001-03-13 University Of Iowa Research Foundation Pathogenesis of cardiomyopathy
WO2010008100A1 (ja) 2008-07-17 2010-01-21 国立大学法人名古屋大学 骨格筋細胞又は骨細胞の誘導法

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Publication number Publication date
US9862929B2 (en) 2018-01-09
JP2014533491A (ja) 2014-12-15
US20140370537A1 (en) 2014-12-18
WO2013073246A1 (en) 2013-05-23

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