JP6021813B2 - 多重増幅および検出 - Google Patents

多重増幅および検出 Download PDF

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JP6021813B2
JP6021813B2 JP2013535508A JP2013535508A JP6021813B2 JP 6021813 B2 JP6021813 B2 JP 6021813B2 JP 2013535508 A JP2013535508 A JP 2013535508A JP 2013535508 A JP2013535508 A JP 2013535508A JP 6021813 B2 JP6021813 B2 JP 6021813B2
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probe
oligonucleotide
amplification
probes
melting
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JP2013540449A (ja
JP2013540449A5 (https=
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グオリアン フー
グオリアン フー
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オキシテック リミテッド
オキシテック リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
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  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013535508A 2010-10-25 2011-10-25 多重増幅および検出 Active JP6021813B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1017978.6 2010-10-25
GBGB1017978.6A GB201017978D0 (en) 2010-10-25 2010-10-25 Multiplex amplification and detection
PCT/GB2011/052068 WO2012056227A2 (en) 2010-10-25 2011-10-25 Multiplex amplification and detection

Publications (3)

Publication Number Publication Date
JP2013540449A JP2013540449A (ja) 2013-11-07
JP2013540449A5 JP2013540449A5 (https=) 2014-12-11
JP6021813B2 true JP6021813B2 (ja) 2016-11-09

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JP2013535508A Active JP6021813B2 (ja) 2010-10-25 2011-10-25 多重増幅および検出

Country Status (5)

Country Link
US (2) US9284607B2 (https=)
EP (2) EP3150726B1 (https=)
JP (1) JP6021813B2 (https=)
GB (1) GB201017978D0 (https=)
WO (1) WO2012056227A2 (https=)

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GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
CN104662172B (zh) * 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
EP2889381A1 (en) * 2013-12-27 2015-07-01 Universite De Liege Detection method for species specific identification of nucleic acid of interest
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
CN108368547B (zh) * 2015-12-15 2022-04-05 Seegene株式会社 与靶核酸序列有关的信号提取
KR102110999B1 (ko) * 2016-01-26 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
US20200157601A1 (en) * 2016-12-02 2020-05-21 Congen Biotechnologie Gmbh Probe for detection of snps
KR20200115552A (ko) * 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
WO2020031156A1 (en) 2018-08-09 2020-02-13 Speedx Pty Ltd Multiplex detection of nucleic acids
AU2020256848A1 (en) * 2020-06-30 2022-10-27 Speedx Pty Ltd Multiplex detection of nucleic acids using mixed reporters
CZ2024298A3 (cs) * 2024-08-02 2026-03-11 Hola Biolabs s.r.o. Detekce alespoň jedné cílové sekvence nukleové kyseliny a kit pro detekci

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EP0232967B1 (en) 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
ATE332398T1 (de) 2000-08-11 2006-07-15 Univ Utah Res Found Einfach markierte oligonukleotidsonden
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
CA2516299A1 (en) 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
EP1709200B1 (en) 2003-12-03 2012-05-09 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP1799853A2 (en) 2004-09-24 2007-06-27 Universität Bern Molecular beacons
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
AU2006204087B2 (en) 2005-01-03 2010-09-16 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
WO2007045890A1 (en) 2005-10-21 2007-04-26 Primerdesign Ltd Probe
US8252558B2 (en) 2006-10-09 2012-08-28 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
DE602006008150D1 (de) * 2006-10-12 2009-09-10 Bio Rad Pasteur Doppelsträngige Sonden zur Fluoreszenzdetektion von Nukleinsäuren
GB0703997D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
CA2680121C (en) * 2007-03-08 2016-05-10 Idaho Technology Primers for melting curve analysis in methods of nucleic acid analysis
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GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection

Also Published As

Publication number Publication date
EP3150726A1 (en) 2017-04-05
EP3150726B1 (en) 2019-08-28
US10400276B2 (en) 2019-09-03
GB201017978D0 (en) 2010-12-08
JP2013540449A (ja) 2013-11-07
WO2012056227A2 (en) 2012-05-03
WO2012056227A3 (en) 2012-11-22
US9284607B2 (en) 2016-03-15
EP2633072A2 (en) 2013-09-04
US20160289751A1 (en) 2016-10-06
US20130267434A1 (en) 2013-10-10

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