JP6013519B2 - 統合された電気化学的免疫測定に基づくマイクロ流体デバイス、及びその基板 - Google Patents
統合された電気化学的免疫測定に基づくマイクロ流体デバイス、及びその基板 Download PDFInfo
- Publication number
- JP6013519B2 JP6013519B2 JP2014561524A JP2014561524A JP6013519B2 JP 6013519 B2 JP6013519 B2 JP 6013519B2 JP 2014561524 A JP2014561524 A JP 2014561524A JP 2014561524 A JP2014561524 A JP 2014561524A JP 6013519 B2 JP6013519 B2 JP 6013519B2
- Authority
- JP
- Japan
- Prior art keywords
- microfluidic device
- substrate
- reagent
- sensor
- working electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000758 substrate Substances 0.000 title claims description 52
- 238000003018 immunoassay Methods 0.000 title claims description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 62
- 239000012491 analyte Substances 0.000 claims description 28
- 229920000307 polymer substrate Polymers 0.000 claims description 20
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 239000002086 nanomaterial Substances 0.000 claims description 17
- -1 polyethylene Polymers 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 13
- 210000004369 blood Anatomy 0.000 claims description 12
- 239000008280 blood Substances 0.000 claims description 12
- 239000002041 carbon nanotube Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 229910021393 carbon nanotube Inorganic materials 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 10
- 229910052737 gold Inorganic materials 0.000 claims description 10
- 239000010931 gold Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 239000000427 antigen Substances 0.000 claims description 7
- 108091007433 antigens Proteins 0.000 claims description 7
- 102000036639 antigens Human genes 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 6
- 229920000642 polymer Polymers 0.000 claims description 6
- 229910052751 metal Inorganic materials 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 239000002105 nanoparticle Substances 0.000 claims description 5
- 210000002966 serum Anatomy 0.000 claims description 5
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 4
- 210000002700 urine Anatomy 0.000 claims description 4
- 108091023037 Aptamer Proteins 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 229910000510 noble metal Inorganic materials 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 229920000728 polyester Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 239000004820 Pressure-sensitive adhesive Substances 0.000 claims description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 2
- 230000021523 carboxylation Effects 0.000 claims description 2
- 238000006473 carboxylation reaction Methods 0.000 claims description 2
- 229910052763 palladium Inorganic materials 0.000 claims description 2
- 229920002492 poly(sulfone) Polymers 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 229920000515 polycarbonate Polymers 0.000 claims description 2
- 239000004417 polycarbonate Substances 0.000 claims description 2
- 229920000570 polyether Polymers 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 2
- 239000004800 polyvinyl chloride Substances 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 26
- 238000003556 assay Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 12
- 238000002965 ELISA Methods 0.000 description 11
- 238000005259 measurement Methods 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 239000007787 solid Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000002071 nanotube Substances 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 238000010256 biochemical assay Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229920001577 copolymer Polymers 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 230000008021 deposition Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002848 electrochemical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000608 laser ablation Methods 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 238000004377 microelectronic Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 238000007650 screen-printing Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004544 sputter deposition Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000005062 Polybutadiene Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 229920000180 alkyd Polymers 0.000 description 1
- XYLMUPLGERFSHI-UHFFFAOYSA-N alpha-Methylstyrene Chemical compound CC(=C)C1=CC=CC=C1 XYLMUPLGERFSHI-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- MTAZNLWOLGHBHU-UHFFFAOYSA-N butadiene-styrene rubber Chemical compound C=CC=C.C=CC1=CC=CC=C1 MTAZNLWOLGHBHU-UHFFFAOYSA-N 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000003486 chemical etching Methods 0.000 description 1
- 238000000970 chrono-amperometry Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000006258 conductive agent Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000004070 electrodeposition Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000010329 laser etching Methods 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 230000037230 mobility Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000012299 nitrogen atmosphere Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 229920001084 poly(chloroprene) Polymers 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920001281 polyalkylene Polymers 0.000 description 1
- 229920002857 polybutadiene Polymers 0.000 description 1
- 229920001195 polyisoprene Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920000131 polyvinylidene Polymers 0.000 description 1
- 229920006214 polyvinylidene halide Polymers 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000007261 regionalization Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 239000002094 self assembled monolayer Substances 0.000 description 1
- 239000013545 self-assembled monolayer Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000005476 size effect Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Clinical Laboratory Science (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Dispersion Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Composite Materials (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IB2012/051170 WO2013136115A1 (en) | 2012-03-13 | 2012-03-13 | Biosensor having nanostrucured electrodes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015510136A JP2015510136A (ja) | 2015-04-02 |
| JP6013519B2 true JP6013519B2 (ja) | 2016-10-25 |
Family
ID=46022501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014561524A Expired - Fee Related JP6013519B2 (ja) | 2012-03-13 | 2012-03-13 | 統合された電気化学的免疫測定に基づくマイクロ流体デバイス、及びその基板 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20150047978A1 (enExample) |
| EP (1) | EP2825501A1 (enExample) |
| JP (1) | JP6013519B2 (enExample) |
| CN (1) | CN104203808B (enExample) |
| IN (1) | IN2014DN08308A (enExample) |
| WO (1) | WO2013136115A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012327218C1 (en) | 2011-11-30 | 2015-12-24 | Wellstat Diagnostics, Llc. | Filtration Module |
| US9213043B2 (en) | 2012-05-15 | 2015-12-15 | Wellstat Diagnostics, Llc | Clinical diagnostic system including instrument and cartridge |
| US9075042B2 (en) | 2012-05-15 | 2015-07-07 | Wellstat Diagnostics, Llc | Diagnostic systems and cartridges |
| US9625465B2 (en) | 2012-05-15 | 2017-04-18 | Defined Diagnostics, Llc | Clinical diagnostic systems |
| US10545161B2 (en) | 2013-03-11 | 2020-01-28 | Cue Health Inc. | Systems and methods for detection and quantification of analytes |
| US9623409B2 (en) | 2013-03-11 | 2017-04-18 | Cue Inc. | Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes |
| CN112782393B (zh) | 2013-03-11 | 2024-11-19 | 克忧健康公司 | 用于检测和量化分析物的系统和方法 |
| USD745423S1 (en) | 2014-05-12 | 2015-12-15 | Cue Inc. | Automated analyzer test cartridge and sample collection device for analyte detection |
| US11009479B2 (en) * | 2014-05-27 | 2021-05-18 | Case Western Reserve University | Systems and methods for the detection of HbA1c |
| US10883956B2 (en) * | 2014-05-27 | 2021-01-05 | Case Western Reserve University | Electrochemical sensor for analyte detection |
| JP2016061769A (ja) * | 2014-09-22 | 2016-04-25 | 株式会社東芝 | 塩基配列検出チップ |
| JP6931035B2 (ja) * | 2014-09-22 | 2021-09-01 | キヤノンメディカルシステムズ株式会社 | 塩基配列検出チップ |
| CN107075433A (zh) * | 2014-09-22 | 2017-08-18 | 东芝医疗系统株式会社 | 核酸检查装置 |
| JP6927879B2 (ja) | 2015-01-14 | 2021-09-01 | ピクセル メディカル テクノロジーズ リミテッド | 試料流体分析用の使い捨てカートリッジ |
| AU2016243036B2 (en) | 2015-04-03 | 2022-02-17 | Abbott Laboratories | Devices and methods for sample analysis |
| WO2016161400A1 (en) * | 2015-04-03 | 2016-10-06 | Abbott Laboratories | Devices and methods for sample analysis |
| EP3325153B1 (en) | 2015-07-17 | 2020-03-18 | Cue Health Inc. | Sample analysis cartridge |
| CN105929153B (zh) * | 2016-04-26 | 2017-08-25 | 哈尔滨工业大学(威海) | 一种黄曲霉毒素b1金纳米井阵列免疫电极的制备方法 |
| US11237161B2 (en) | 2017-01-25 | 2022-02-01 | Cue Health Inc. | Systems and methods for enhanced detection and quantification of analytes |
| CN107045068A (zh) * | 2017-04-14 | 2017-08-15 | 刘锦 | 基于微流控纸芯片的便携式生理指标检测仪及其检测方法 |
| CN111323578B (zh) * | 2020-02-28 | 2023-11-03 | 京东方科技集团股份有限公司 | 一种微流控结构、微流控芯片及检测方法 |
| CN112326750B (zh) * | 2020-11-06 | 2023-07-11 | 吉林医药学院 | 一种基于微流控方法的甲型流感病毒检测装置与检测方法 |
| US20240295524A1 (en) * | 2021-08-31 | 2024-09-05 | Virginia Commonwealth University | Self-calibrated electrochemical sensors |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5399256A (en) * | 1994-01-07 | 1995-03-21 | Bioanalytical Systems, Inc. | Electrochemical detector cell |
| US6673533B1 (en) * | 1995-03-10 | 2004-01-06 | Meso Scale Technologies, Llc. | Multi-array multi-specific electrochemiluminescence testing |
| JPH09101283A (ja) * | 1995-10-03 | 1997-04-15 | Nippon Telegr & Teleph Corp <Ntt> | 電気化学検出器およびその製造方法 |
| US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| US20060228723A1 (en) * | 2002-01-16 | 2006-10-12 | Keith Bradley | System and method for electronic sensing of biomolecules |
| US7419821B2 (en) | 2002-03-05 | 2008-09-02 | I-Stat Corporation | Apparatus and methods for analyte measurement and immunoassay |
| EP1514097A1 (en) * | 2002-06-19 | 2005-03-16 | Becton, Dickinson and Company | Microfabricated sensor arrays |
| CN102620959B (zh) | 2002-12-26 | 2015-12-16 | 梅索磅秤技术有限公司 | 检定盒及其使用方法 |
| GB0423961D0 (en) * | 2004-10-28 | 2004-12-01 | Cole Polytechnique Federale De | Adsorption monitoring device |
| JP4597091B2 (ja) * | 2006-05-24 | 2010-12-15 | 株式会社日立ハイテクノロジーズ | 生化学分析装置及びそれに用いる検査カートリッジ |
| JP2009002939A (ja) * | 2007-05-18 | 2009-01-08 | Mitsubishi Kagaku Iatron Inc | アンペロメトリック型バイオセンサ |
| JP5026873B2 (ja) * | 2007-07-04 | 2012-09-19 | 株式会社船井電機新応用技術研究所 | 酵素電極、酵素電極の製造方法及び酵素センサ |
| JP2009216516A (ja) * | 2008-03-10 | 2009-09-24 | Sumitomo Electric Ind Ltd | バイオセンサチップおよびその製造方法 |
| EP2261648B1 (en) * | 2008-03-17 | 2014-05-21 | Mitsubishi Chemical Medience Corporation | Electric analysis method |
| GB0815472D0 (en) | 2008-08-22 | 2008-10-01 | Iti Scotland Ltd | Microfluidic system |
| JP2010156605A (ja) * | 2008-12-26 | 2010-07-15 | Mitsubishi Chemical Medience Corp | 電気的分析方法 |
| US20110266151A1 (en) * | 2010-04-23 | 2011-11-03 | Fredrik Jansson | Microfluidic systems with electronic wettability switches |
| US8808519B2 (en) * | 2011-12-14 | 2014-08-19 | Hamilton Sundstrand Space Systems International | Microfluidic device |
-
2012
- 2012-03-13 US US14/384,538 patent/US20150047978A1/en not_active Abandoned
- 2012-03-13 CN CN201280071882.XA patent/CN104203808B/zh not_active Expired - Fee Related
- 2012-03-13 EP EP12717851.5A patent/EP2825501A1/en not_active Withdrawn
- 2012-03-13 WO PCT/IB2012/051170 patent/WO2013136115A1/en not_active Ceased
- 2012-03-13 IN IN8308DEN2014 patent/IN2014DN08308A/en unknown
- 2012-03-13 JP JP2014561524A patent/JP6013519B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP2825501A1 (en) | 2015-01-21 |
| CN104203808B (zh) | 2017-06-09 |
| CN104203808A (zh) | 2014-12-10 |
| US20150047978A1 (en) | 2015-02-19 |
| WO2013136115A1 (en) | 2013-09-19 |
| IN2014DN08308A (enExample) | 2015-05-15 |
| JP2015510136A (ja) | 2015-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6013519B2 (ja) | 統合された電気化学的免疫測定に基づくマイクロ流体デバイス、及びその基板 | |
| KR100885074B1 (ko) | 미세유로형 센서 복합 구조물 | |
| Gaikwad et al. | Advances in point-of-care diagnostic devices in cancers | |
| US8614056B2 (en) | Microfluidic method for measurement or detection involving cells or biomolecules | |
| JP5798647B2 (ja) | 磁性粒子を有する多数区画装置 | |
| CN103282124B (zh) | 具有整合的样品稀释的样品计量装置和测定装置 | |
| CN103282125A (zh) | 具有整合的样品稀释的样品计量装置和测定装置 | |
| Moazeni et al. | Peptide modified paper based impedimetric immunoassay with nanocomposite electrodes as a point-of-care testing of Alpha-fetoprotein in human serum | |
| JP2006516721A (ja) | 多孔質層上に試薬を含む複層化された電気化学系微小流体センサー | |
| Hervás et al. | Electrochemical immunosensing on board microfluidic chip platforms | |
| JP2014503832A (ja) | 微小流体アッセイ装置および方法 | |
| KR102007164B1 (ko) | 다중 미세유로를 구비한 하이브리드 신속 진단 키트 | |
| US20200284788A1 (en) | Patterned Plasmonic Nanoparticle Arrays for Multiplexed, Microfluidic Biosensing Assays | |
| WO2010086772A1 (en) | System and method for assay | |
| CN102762971B (zh) | 纳米流体生物传感器及其对于快速测量溶液中的生物分子相互作用的应用及方法 | |
| US20230264192A1 (en) | Microfluidic electrochemical analyte detectors | |
| Fong Lei | Recent developments and patents on biological sensing using nanoparticles in microfluidic systems | |
| Karaboğa et al. | Microfluidic based biosensors and applications | |
| WO2016087957A1 (en) | Multiplexed microfluidic device | |
| Jani et al. | Lab‐on‐a‐Chip Devices for Point‐of‐Care Infectious Diseases Diagnostics | |
| Ghoorchian et al. | Lab-on-a-chip miniaturized analytical devices | |
| US20080311679A1 (en) | Biosensor Device | |
| US12285760B2 (en) | Analytical device and reaction chamber | |
| JP2007263706A (ja) | バイオアッセイ用マイクロチップ | |
| Karaboğa et al. | 11 Microfuidic Based |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20150313 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20151204 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20151222 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160315 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160830 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160921 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6013519 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |