JP5996868B2 - 高感度アッセイのためのパルス磁気作動 - Google Patents
高感度アッセイのためのパルス磁気作動 Download PDFInfo
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- JP5996868B2 JP5996868B2 JP2011531589A JP2011531589A JP5996868B2 JP 5996868 B2 JP5996868 B2 JP 5996868B2 JP 2011531589 A JP2011531589 A JP 2011531589A JP 2011531589 A JP2011531589 A JP 2011531589A JP 5996868 B2 JP5996868 B2 JP 5996868B2
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R33/00—Arrangements or instruments for measuring magnetic variables
- G01R33/12—Measuring magnetic properties of articles or specimens of solids or fluids
- G01R33/1269—Measuring magnetic properties of articles or specimens of solids or fluids of molecules labeled with magnetic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2400/00—Moving or stopping fluids
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- B03C2201/00—Details of magnetic or electrostatic separation
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
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Description
Claims (11)
- バイオセンサカートリッジ中の磁性物体又は磁化可能物体の運動を制御するための方法であって、
(a)側方にのびるセンサ表面を持つバイオセンサカートリッジと、前記センサ表面の下に配置された少なくとも2つの磁場発生手段及び前記センサ表面の上に配置された少なくとも1つの磁場発生手段とを設けるステップと、
(b)前記発生した磁場が、前記磁性物体又は磁化可能物体を、前記センサ表面に垂直に、前記センサ表面に対して進退させて交互に移動させるように、前記磁場発生手段を交互に作動させるステップであって、前記交互作動のパルス長は、前記側方にのびるセンサ表面に沿った磁性物体又は磁化可能物体の側方運動が回避されるように調節され、前記センサ表面の下に配置された少なくとも2つの磁場発生手段が、前記磁性物体又は磁化可能物体を前記センサ表面へ近づけるために反対の極性で同時に作動されるか、又は、前記磁性物体又は磁化可能物体を前記センサ表面から離すために、前記センサ表面の下に配置された少なくとも2つの磁場発生手段が同時にオフにされ、前記センサ表面の上に配置された少なくとも1つの磁場発生手段がオンにされる、ステップと、
を有する方法。 - 検出パルスを与えるステップをさらに有し、その間に前記磁性物体又は磁化可能物体の重力方向の運動が回避されるように前記磁場が調節される、請求項1に記載の方法。
- 前記センサ表面に結合した前記磁性物体又は磁化可能物体が前記検出パルス中に検出される、請求項2に記載の方法。
- 前記交互作動の周波数が1Hzより大きく10Hzより低く、又は2Hzより大きく6Hzより低く、又は3乃至5Hzであり、又は4Hzである、請求項1に記載の方法。
- 前記交互作動の周期中に、前記センサ表面に位置する磁性物体又は磁化可能物体が、最初に所定の距離だけ前記表面から離れ、その後同じ所定の距離だけ前記表面に近づく、請求項1に記載の方法。
- 前記交互作動の周期内に、前記磁性物体又は磁化可能物体を前記センサ表面へ近づけるための第1のパルス長を持つ磁場を発生させ、
その後、前記磁性物体又は磁化可能物体を前記センサ表面から離すための第2のパルス長を持つ磁場を発生させ、前記第1のパルス長と前記第2のパルス長の間に発生磁場のない時間間隔を発生させる、請求項1に記載の方法。 - 前記発生磁場強度を持つ前記第1のパルス長と、前記発生磁場強度を持つ前記第2のパルス長が、前記磁性物体又は磁化可能物体が前記センサ表面から離れる運動及び前記センサ表面に近づく運動の位相中に同じ距離だけ移動するように調節される、請求項6に記載の方法。
- 前記第1及び/又は第2のパルス長が前記周期の少なくとも5%、又は少なくとも20%、又は25%、及び又は40%未満である、請求項6に記載の方法。
- 前記第1のパルス長と前記第2のパルス長の間の前記時間間隔が、前記周期の30%より大きく、又は40%より大きく、又は50%より大きい、請求項6に記載の方法。
- バイオセンサシステムであって、
(a)側方にのびるセンサ表面を持つバイオセンサカートリッジと、
(b)前記センサ表面の下に配置された少なくとも2つの磁場発生手段及び前記センサ表面の上に配置された少なくとも1つの磁場発生手段と、
(c)前記発生した磁場が、磁性物体又は磁化可能物体を前記センサ表面に垂直に、前記センサ表面に対して進退させて交互に移動させるように、前記磁場発生手段を交互に作動させるための駆動ユニットであって、前記駆動ユニットは前記センサ表面に沿った前記磁性物体又は磁化可能物体の側方運動を回避する前記交互作動のパルス長を発生させる、駆動ユニットと、
を有し、
前記駆動ユニットが、前記磁性物体又は磁化可能物体を前記センサ表面に近づけるために、前記センサ表面の下に配置された少なくとも2つの磁場発生手段を反対の極性で同時に作動させ、及び/又は、前記磁性物体又は磁化可能物体を前記センサ表面から離すために、前記センサ表面の下に配置された少なくとも2つの磁場発生手段を同時にオフにさせ、前記センサ表面の上に配置された少なくとも1つの磁場発生手段をオンにさせる、
バイオセンサシステム。 - 前記バイオセンサカートリッジが取り外し可能に配置される、請求項10に記載のバイオセンサシステム。
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EP08166901.2 | 2008-10-17 | ||
EP08166901 | 2008-10-17 | ||
PCT/IB2009/054374 WO2010044007A2 (en) | 2008-10-17 | 2009-10-06 | Pulsed magnetic actuation for sensitive assays |
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JP2012506045A JP2012506045A (ja) | 2012-03-08 |
JP5996868B2 true JP5996868B2 (ja) | 2016-09-21 |
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US (1) | US10794903B2 (ja) |
EP (1) | EP2340434B1 (ja) |
JP (1) | JP5996868B2 (ja) |
CN (1) | CN102187241B (ja) |
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EP2362219A1 (en) | 2010-02-19 | 2011-08-31 | Nxp B.V. | Sensor measuring method and sensing apparatus |
US20110262989A1 (en) * | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
EP2614365B1 (en) * | 2010-09-09 | 2019-02-20 | Koninklijke Philips N.V. | A method and a device for attracting magnetic particles to a surface |
US10031132B2 (en) | 2011-10-20 | 2018-07-24 | Minicare B.V. | Magnetic particle detection with incubation period |
EP2773946B1 (en) * | 2011-11-03 | 2021-08-25 | Siemens Healthineers Nederland B.V. | Detection of surface-bound magnetic particles |
RU2653145C2 (ru) | 2012-09-04 | 2018-05-07 | Конинклейке Филипс Н.В. | Измерительное устройство и способ отбора образцов |
JP7455529B2 (ja) | 2019-08-01 | 2024-03-26 | キヤノンメディカルシステムズ株式会社 | 検体測定装置及び検体測定装置の制御方法 |
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US7998746B2 (en) | 2000-08-24 | 2011-08-16 | Robert Otillar | Systems and methods for localizing and analyzing samples on a bio-sensor chip |
WO2003054566A1 (en) | 2001-12-21 | 2003-07-03 | Koninklijke Philips Electronics N.V. | Magnetoresistive sensing device, system and method for determining a density of magnetic particles in fluid |
EP1456658B1 (en) | 2001-12-21 | 2006-05-17 | Koninklijke Philips Electronics N.V. | Sensor and method for measuring the areal density of magnetic nanoparticles on a micro-array |
JP4240303B2 (ja) | 2002-01-29 | 2009-03-18 | 旭化成株式会社 | バイオセンサ、磁性分子測定方法、及び、測定対象物測定方法 |
JP4399211B2 (ja) * | 2002-12-21 | 2010-01-13 | 株式会社ハイニックスセミコンダクター | バイオセンサー |
EP1697755A1 (en) | 2003-07-30 | 2006-09-06 | Koninklijke Philips Electronics N.V. | On-chip magnetic sensor device with suppressed cross-talk |
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EP1751534A1 (en) | 2004-05-18 | 2007-02-14 | Koninklijke Philips Electronics N.V. | Magnetic rotation to improve signal-over-background in biosensing |
PT1781820T (pt) * | 2004-08-20 | 2018-07-10 | Magnomics Sa | Um dispositivo bioeletrónico |
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- 2009-10-06 CN CN200980140699.9A patent/CN102187241B/zh active Active
- 2009-10-06 RU RU2011119631/15A patent/RU2520607C2/ru not_active IP Right Cessation
- 2009-10-06 WO PCT/IB2009/054374 patent/WO2010044007A2/en active Application Filing
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EP2340434A2 (en) | 2011-07-06 |
RU2011119631A (ru) | 2012-11-27 |
WO2010044007A2 (en) | 2010-04-22 |
US10794903B2 (en) | 2020-10-06 |
WO2010044007A3 (en) | 2010-09-16 |
EP2340434B1 (en) | 2014-03-19 |
CN102187241B (zh) | 2015-11-25 |
JP2012506045A (ja) | 2012-03-08 |
RU2520607C2 (ru) | 2014-06-27 |
CN102187241A (zh) | 2011-09-14 |
US20110244596A1 (en) | 2011-10-06 |
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