JP5909444B2 - 核酸分析 - Google Patents

核酸分析 Download PDF

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JP5909444B2
JP5909444B2 JP2012520829A JP2012520829A JP5909444B2 JP 5909444 B2 JP5909444 B2 JP 5909444B2 JP 2012520829 A JP2012520829 A JP 2012520829A JP 2012520829 A JP2012520829 A JP 2012520829A JP 5909444 B2 JP5909444 B2 JP 5909444B2
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nucleic acid
microvesicles
rna
urine
concentration
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JP2012533308A (ja
JP2012533308A5 (https=
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レイルアタ エム． ルッソ
レイルアタ エム． ルッソ
ケビン シー． ミランダ
ケビン シー． ミランダ
ヨハン スコッグ
ヨハン スコッグ
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ザ ジェネラル ホスピタル コーポレイション
ザ ジェネラル ホスピタル コーポレイション
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D249/00—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms
    • C07D249/02—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D249/08—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/12—Antidiuretics, e.g. drugs for diabetes insipidus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2012520829A 2009-07-16 2010-07-16 核酸分析 Active JP5909444B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22610609P 2009-07-16 2009-07-16
US22602509P 2009-07-16 2009-07-16
US61/226,025 2009-07-16
US61/226,106 2009-07-16
PCT/US2010/042365 WO2011009104A1 (en) 2009-07-16 2010-07-16 Nucleic acid analysis

Related Child Applications (1)

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JP2015246006A Division JP2016116512A (ja) 2009-07-16 2015-12-17 核酸分析

Publications (3)

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JP2012533308A JP2012533308A (ja) 2012-12-27
JP2012533308A5 JP2012533308A5 (https=) 2013-08-29
JP5909444B2 true JP5909444B2 (ja) 2016-04-26

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JP2012520829A Active JP5909444B2 (ja) 2009-07-16 2010-07-16 核酸分析
JP2015246006A Pending JP2016116512A (ja) 2009-07-16 2015-12-17 核酸分析
JP2017086218A Pending JP2017131244A (ja) 2009-07-16 2017-04-25 核酸分析

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JP2017086218A Pending JP2017131244A (ja) 2009-07-16 2017-04-25 核酸分析

Country Status (13)

Country Link
US (3) US20110195426A1 (https=)
EP (1) EP2454588B1 (https=)
JP (3) JP5909444B2 (https=)
KR (2) KR20180029105A (https=)
CN (2) CN105779436A (https=)
AU (1) AU2010273928A1 (https=)
BR (1) BR112012001077A2 (https=)
CA (1) CA2768371A1 (https=)
DK (1) DK2454588T3 (https=)
ES (1) ES2547715T3 (https=)
IN (1) IN2012DN00866A (https=)
SG (2) SG2014014617A (https=)
WO (1) WO2011009104A1 (https=)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3061952C (en) * 2006-03-09 2022-07-19 Aethlon Medical, Inc. Extracorporeal removal of microvesicular particles
US11266736B2 (en) 2008-04-17 2022-03-08 Vin De Bona Trading Company Pte Ltd Method of painting micro vesicles
EP2475989A4 (en) 2009-09-09 2013-02-27 Gen Hospital Corp USE OF MICROVESICLES IN THE ANALYSIS OF KRAS MUTATIONS
WO2011031877A1 (en) 2009-09-09 2011-03-17 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
JP5755326B2 (ja) * 2010-06-11 2015-07-29 日立化成株式会社 腎機能の特徴を決定する方法
US20140045915A1 (en) 2010-08-31 2014-02-13 The General Hospital Corporation Cancer-related biological materials in microvesicles
JP6219721B2 (ja) * 2010-11-10 2017-10-25 エキソソーム ダイアグノスティックス インコーポレイテッド 核酸含有粒子の単離および該粒子からの核酸の抽出のための方法
US20120196285A1 (en) * 2011-01-31 2012-08-02 Esoterix Genetic Laboratories, Llc Methods for Enriching Microparticles or Nucleic Acids Using Binding Molecules
CA2835641C (en) * 2011-05-11 2019-05-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
US9719129B2 (en) 2011-06-10 2017-08-01 Hitachi Chemical Co., Ltd. Methods for isolating vesicles from biological fluids
EP3492606B1 (en) 2011-08-22 2023-10-04 Exosome Diagnostics, Inc. Urine biomarkers
EP2776587A4 (en) * 2011-11-10 2015-07-15 Exosome Diagnostics Inc LIQUOR ASSAY
CN104995311A (zh) 2012-08-30 2015-10-21 外来体诊断公司 用于核酸测定的对照
CA2887058C (en) 2012-10-03 2022-02-22 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
WO2014055687A1 (en) * 2012-10-05 2014-04-10 Hitachi Chemical Co., Ltd. Urine exosome mrnas and methods of using same to detect diabetic nephropathy
DK2906717T3 (en) 2012-10-11 2019-04-01 Vin De Bona Trading Company Pte Ltd PROCEDURE FOR PAINTING MICROVESICS
CN105209881B (zh) * 2013-03-13 2021-01-22 迈阿密大学 从细胞培养上清液和生物流体分离并纯化微泡的方法
US12558316B2 (en) 2013-03-13 2026-02-24 University Of Miami Methods and compositions for the treatment of epidermolysis bullosa
JP6297676B2 (ja) 2013-05-06 2018-03-20 日立化成株式会社 標的分子を捕捉するためのデバイス及び方法
AU2014305994B2 (en) 2013-08-06 2019-02-21 Exosome Diagnostics, Inc. Urine biomarker cohorts, gene expression signatures, and methods of use thereof
US10607717B2 (en) * 2013-11-06 2020-03-31 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method for subtyping lymphoma types by means of expression profiling
EP3077528B1 (en) * 2013-12-02 2018-08-22 Albert-Ludwigs-Universität Freiburg Process for the diagnosis of cancer by using exosomes
US10844436B2 (en) 2014-04-01 2020-11-24 Cornell University Use of double-stranded DNA in exosomes: a novel biomarker in cancer detection
CA2946384A1 (en) * 2014-04-22 2015-10-29 Trovagene, Inc. Concentrating nucleic acids in urine
CN104031915A (zh) * 2014-06-17 2014-09-10 复旦大学附属肿瘤医院 用于结直肠癌预后的基因片段及其应用
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
WO2016172598A1 (en) * 2015-04-22 2016-10-27 The Broad Institute Inc. Exosomes and uses thereof
GB2539497A (en) 2015-06-19 2016-12-21 Airbus Operations Ltd A Rotational Joint For An Aircraft Folding Wing
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
WO2018057820A1 (en) 2016-09-21 2018-03-29 Predicine, Inc. Systems and methods for combined detection of genetic alterations
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
AU2017263886B2 (en) * 2016-05-13 2023-11-09 Exosome Diagnostics, Inc. Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids
US11891664B2 (en) 2017-05-17 2024-02-06 Exosome Diagnostics, Inc. Methods of detecting renal transplant rejection using microvesicle nucleic acid biomarkers
US11899024B2 (en) 2017-07-12 2024-02-13 Exosome Diagnostics, Inc. Treatment and diagnosis of parkinson's disease using isolated and enriched populations of biofluid-derived extracellular vesicles
JP7069587B2 (ja) * 2017-07-31 2022-05-18 東ソー株式会社 分析機器の洗浄方法
WO2019116234A1 (en) * 2017-12-12 2019-06-20 Nestec S.A. Methods for determining microbiome ribosomal rna composition changes
JP7444386B2 (ja) * 2018-06-07 2024-03-06 株式会社Ｌｓｉメディエンス ヒト尿からのマイクロベシクルの分離方法及び分析方法
WO2020106853A1 (en) 2018-11-20 2020-05-28 Exosome Diagnostics, Inc. Compositions and methods for internal controls of microvesicle isolations
CN109856259A (zh) * 2018-12-29 2019-06-07 南通大学附属医院 一种血清胰腺癌外泌体特异蛋白及其再验证过程
CN114958834A (zh) * 2020-03-09 2022-08-30 中山大学 一种adar蛋白细胞内高效结合底物及应用
CA3180572A1 (en) 2020-05-29 2021-12-02 Johan Skog Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
KR20230051519A (ko) 2020-08-15 2023-04-18 리제너론 파마슈티칼스 인코포레이티드 칼시토닌 수용체 (calcr)를 인코딩하는 변이체 핵산 분자를 갖는 대상체에서 비만의 치료
US20250075258A1 (en) 2021-07-16 2025-03-06 Exosome Diagnostics, Inc. Methods of detecting sjogren's syndrome using salivary exosomes
JP2025506701A (ja) 2022-02-18 2025-03-13 エクソサム ダイアグノスティクス，インコーポレイティド 腎障害の特定および治療における微小胞シグネチャーの使用
CN114480656B (zh) * 2022-03-07 2023-12-26 温州医科大学附属眼视光医院 Slc12a3和/或slc12a9在作为葡萄膜黑色素瘤治疗和预后检测指标中的应用
WO2024054572A1 (en) 2022-09-07 2024-03-14 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5840867A (en) 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
AU645915B2 (en) 1991-07-23 1994-01-27 F. Hoffmann-La Roche Ag Improvements in the in situ PCR
US5582981A (en) 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
ATE471150T1 (de) * 1996-03-26 2010-07-15 Kopreski Michael S Methoden aus plasma oder serum extrahierte extrazelluraere rna zur diagnoseüberwachung oder evaluation von krebs verwenden
DE69738206T2 (de) 1996-11-06 2008-07-17 Sequenom, Inc., San Diego DNA-Diagnostik mittels Massenspektrometrie
US6994960B1 (en) 1997-05-28 2006-02-07 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US6004755A (en) 1998-04-07 1999-12-21 Incyte Pharmaceuticals, Inc. Quantitative microarray hybridizaton assays
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6870021B2 (en) * 2000-01-28 2005-03-22 Mip Technologies Ab Functional monomers for molecular recognition and catalysis
US20040241176A1 (en) * 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
US6913879B1 (en) 2000-07-10 2005-07-05 Telechem International Inc. Microarray method of genotyping multiple samples at multiple LOCI
US6525154B1 (en) 2000-07-20 2003-02-25 The Regents Of The University Of California Molecular imprinting for the recognition of peptides in aqueous solution
JP4162494B2 (ja) * 2001-05-11 2008-10-08 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 筋緊張性ジストロフィー２型に関連するイントロン、および使用方法
AU2002310296A1 (en) * 2001-06-05 2002-12-16 Gene Logic, Inc. Rna purification methods
US6696271B2 (en) 2001-08-23 2004-02-24 The Regents Of The University Of California Frozen tissue microarray technology for analysis of RNA, DNA, and proteins
WO2003023065A1 (en) 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
US20030162190A1 (en) 2001-11-15 2003-08-28 Gorenstein David G. Phosphoromonothioate and phosphorodithioate oligonucleotide aptamer chip for functional proteomics
AU2003302146B2 (en) 2002-06-26 2008-06-26 Cold Spring Harbor Laboratory Methods and compositions for determining methylation profiles
JP2005537002A (ja) 2002-09-02 2005-12-08 パムジーン ベー．ベー． 新規な一体化したマイクロアレイ分析
US7141371B2 (en) 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
EP1498144A1 (en) * 2003-07-15 2005-01-19 Universite Pierre Et Marie Curie Paris Vi Extracellular vesicles from non-pathogenic amoebae useful as vehicle for transferring a molecule of interest to an eukaryotic cell
US7384589B2 (en) 2003-08-01 2008-06-10 Lawrence Livermore National Security, Llc Nanoscale molecularly imprinted polymers and method thereof
US8021847B2 (en) * 2004-06-02 2011-09-20 Proxy Life Science Holdings, Inc. Microvesicle-based compositions and methods
WO2005121369A2 (en) * 2004-06-02 2005-12-22 Sourcepharm, Inc. Rna-containing microvesicles and methods therefor
KR20070058441A (ko) * 2004-07-01 2007-06-08 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 면역억제성 엑소솜
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
EP1869221A2 (en) 2005-04-15 2007-12-26 Cedars-Sinai Medical Center Flow-cytometric heteroduplex analysis for detection of genetic alterations
WO2006119439A2 (en) * 2005-05-03 2006-11-09 Althea Technologies, Inc. Compositions and methods for the analysis of degraded nucleic acids
WO2007100699A2 (en) * 2006-02-24 2007-09-07 Novartis Ag Microparticles containing biodegradable polymer and cationic polysaccharide for use in immunogenic compositions
JP2008035779A (ja) * 2006-08-07 2008-02-21 Mitsubishi Rayon Co Ltd 核酸の分解度を測定する方法と核酸アレイ
US20080287669A1 (en) * 2007-05-16 2008-11-20 Braman Jeffrey C Methods and compositions for identifying compounds useful in nucleic acid purification
WO2009015357A1 (en) * 2007-07-25 2009-01-29 University Of Louisville Research Foundation, Inc. Exosome-associated microrna as a diagnostic marker
JP5769417B2 (ja) * 2007-08-16 2015-08-26 ザ ロイヤル インスティチューション フォー ザ アドバンスメント オブ ラーニング／マギル ユニバーシティ 腫瘍細胞由来微小胞
WO2009030029A1 (en) * 2007-09-05 2009-03-12 Laurentian University Method of using tumour rna integrity to measure response to chemotherapy in cancer patients
ES2575868T3 (es) * 2007-09-14 2016-07-01 The Ohio State University Research Foundation Expresión de miARN en microvesículas de sangre periférica humana y sus usos
EP2245199B1 (en) * 2008-02-01 2013-11-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions

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AU2010273928A1 (en) 2012-02-09
KR20120037992A (ko) 2012-04-20
BR112012001077A2 (pt) 2020-08-11
SG2014014617A (en) 2014-05-29
HK1173213A1 (en) 2013-05-10
DK2454588T3 (en) 2015-09-28
KR20180029105A (ko) 2018-03-19
CA2768371A1 (en) 2011-01-20
CN105779436A (zh) 2016-07-20
EP2454588A4 (en) 2012-12-12
SG177677A1 (en) 2012-02-28
WO2011009104A1 (en) 2011-01-20
EP2454588B1 (en) 2015-07-15
JP2016116512A (ja) 2016-06-30
HK1171083A1 (zh) 2013-03-15
ES2547715T3 (es) 2015-10-08
US20110195426A1 (en) 2011-08-11
AU2010273928A2 (en) 2012-02-09
JP2012533308A (ja) 2012-12-27
US20170114389A1 (en) 2017-04-27
US20160348095A1 (en) 2016-12-01
CN102498397A (zh) 2012-06-13
CN102498397B (zh) 2016-04-06
IN2012DN00866A (https=) 2015-07-10
JP2017131244A (ja) 2017-08-03

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