JP5874789B2 - Transcript - Google Patents

Description

  The present invention relates to a transfer body that is used by being attached to an article such as a credit card in the field of forgery prevention, and more specifically includes a highly reliable authentication information medium that is difficult to counterfeit, The present invention relates to a transfer body having a high ability to guarantee the authenticity of the article.

  The main use of the transfer body of the present invention is a transfer foil, a transfer ribbon, a hologram sheet or the like used in the field of forgery prevention, and specifically, when a counterfeit card such as a credit card is used Items that may cause damage to the holder or card company, driver's license, employee ID card, membership card ID card, entrance examination voucher, passport, banknote, gift certificate, point card, stock certificate, securities, lottery Tickets, horse tickets, bankbooks, boarding tickets, passports, air tickets, admission tickets for various events, play tickets, prepaid cards for transportation and various telephones. That is, the transfer body of the present invention is an information recording medium that holds information having economic or social value, information such as identification of the person, and the authenticity of the recording medium itself for the purpose of preventing damage caused by forgery. It is applied to an article that is desired to have a function capable of identifying the Furthermore, the present invention can be preferably applied to an article for which it is desired to improve the durability of the visual image, that is, scratch resistance, solvent resistance, and the like.

  In addition to the uses described above, expensive products such as luxury watches, luxury leather products, precious metal products, jewelry, etc., often referred to as luxury brand products, or storage boxes or cases for these expensive products Also, famous brands of mass-produced products, such as audio products, electrical appliances, etc., or tags hung on them, and storage media on which music software, video software, computer software, game software, etc. that are copyrighted works are recorded, Or it can apply also to those cases. In addition, for products that have the ability to identify their authenticity for the purpose of preventing damage caused by counterfeiting, such as printer toner and paper, such as products whose replacement equipment is limited to genuine materials. Can be applied. In addition, those with very high social value that require higher reliability, such as paintings and sculptures by famous creators, antiques and jewelry that are recognized worldwide, 100 Agricultural products that are very difficult to re-produce, such as wines that have been aged for nearly a year, and more expensive because there are only a few genuine ones in the world, or only one genuine one in the world. It can be suitably applied to non-existent documents (contracts, wills, etc.).

  Conventionally, for the purpose of preventing forgery of the article, a hologram that is said to be difficult to manufacture due to the precision of its structure is often applied as one that can be identified as authentic (for example, Patent Document 1). However, since the hologram manufacturing method itself is known and can be precisely processed by that method, when the hologram is merely for visual judgment, there is no difference between a genuine hologram and a forged hologram. It is difficult to distinguish. These authentic identification objects need to identify not only the authenticity of visual confirmation of the hologram image but also the authenticity of the object using a new authenticity identification method.

JP-A-5-155199

  An object of the present invention is to provide a transfer body that solves the above-described problems, is difficult to counterfeit, has a reliable authentication information medium, and has a high ability to guarantee the authenticity of the article.

  As a result of various studies, the inventor is a transfer body in which at least a hologram forming layer, a reflective thin film layer, a nucleic acid-containing ink layer, and an adhesive layer are sequentially laminated on a transfer substrate, and the nucleic acid-containing ink layer Is composed of a nucleic acid-containing ink composition containing a nucleic acid and a fluorescent dye, and is partially provided on the reflective thin film layer. The refractive index of the nucleic acid-containing ink layer and the refractive index of the adhesive layer It was found that a transfer body characterized in that the difference is 0.1 or less achieves the above object.

  In the transfer body of the present invention, a hologram forming layer, a reflective thin film layer, and a nucleic acid-containing ink layer are sequentially laminated on a transfer substrate, and then a nucleic acid-containing ink layer is provided before the adhesive layer is laminated. In order to smooth the unevenness on the reflective thin film layer composed of the portion not provided, a smoothing layer for embedding the recess may be laminated.

In the present invention, the smoothing layer may be formed of the same resin composition as the adhesive layer.
Furthermore, you may have a peeling layer between a transfer base material and a hologram formation layer.
Moreover, you may provide the protective layer which covers the surface of a hologram formation layer between a transfer base material and a hologram formation layer, and when a peeling layer exists between a peeling layer and a hologram formation layer.
In order to detect the position of the portion where the nucleic acid-containing ink layer is provided, a detection mark can also be provided in the hologram forming layer or other layers.
Moreover, an ultraviolet absorber can also be contained in any one layer, in particular, any layer between the nucleic acid-containing ink layer and the transfer substrate.

The transfer body of the present invention has the above specific layer structure, and in particular, since the nucleic acid-containing ink layer is provided between the reflective thin film layer and the adhesive layer, the nucleic acid-containing ink layer peels off and disappears. There is nothing to do.
Further, in the state where the transfer body of the present invention is applied on the article, the nucleic acid-containing ink layer is located inside the hologram forming layer and the reflective thin film layer, so that the partially provided nucleic acid-containing ink layer is The position in the transfer body is concealed by the hologram reproduction image and the reflective thin film, and is hardly visible. Therefore, by providing the nucleic acid-containing ink layer in an extremely small area and setting the concentration of the nucleic acid contained therein to the detection limit, unauthorized acquisition of the nucleic acid contained in the transfer body of the present invention can be prevented.

Furthermore, when the nucleic acid-containing ink layer and the adhesive layer are transparent and the difference between the refractive index of the nucleic acid-containing ink layer and the refractive index of the adhesive layer is 0.1 or less, these boundary lines are reduced. The anti-counterfeiting property is further improved.
In addition, before laminating the adhesive layer, a smoothing layer for embedding the recesses in order to smooth the unevenness on the reflective thin film layer composed of the portion provided with the nucleic acid-containing ink layer and the portion not provided with the nucleic acid-containing ink layer. By laminating the film, it becomes possible to accurately reproduce the thickness of each layer, to obtain a transfer body having a smooth surface without unevenness, and to prevent transferability from being deteriorated. Here, the difference between the refractive index of the nucleic acid-containing ink layer and the refractive index of the smoothing layer is preferably 0.1 or less.

  In the transfer body of the present invention, the nucleic acid-containing ink is embedded in a resin composition that forms an adhesive layer and / or a smoothing layer. Under such conditions, the nucleic acid is kept very stable, thus maintaining the ability to maintain sequence information over a long period of time, i.e., assure the authenticity of the article.

Furthermore, when the transcript of the present invention contains a nucleic acid having a length of n bases (pairs), the nucleic acid can be combined with as many as 4 ⁿ sequences. The present invention can be applied not only to identification of individual information but also to identification of individual information.
In addition, the article to which the transfer body of the present invention is applied has three steps, that is, the first step is to visually check the hologram image, and the second step is simple to detect a specific fluorescence wavelength from a specific position. According to the determination method, in the third stage, the authenticity of the article can be determined by collecting the nucleic acid by partially destroying it and examining whether the nucleic acid has valid sequence information. .

It is a schematic sectional drawing which shows the example about the layer structure of the transfer body concerning this invention.

The above-described present invention will be described in more detail below.
<1> Layer Configuration of Transfer Body of the Present Invention As a transfer body according to the present invention, for example, as shown in FIG. 1, a transfer substrate 1, a hologram forming layer 2, a reflective thin film layer 3, an adhesive layer 4, A transfer body having a basic structure composed of a nucleic acid-containing ink layer 5 partially provided between the reflective thin film layer 3 and the adhesive layer 4 can be exemplified.
As another example, in order to smooth the unevenness on the reflective thin film layer composed of the portion provided with the nucleic acid-containing ink layer and the portion not provided, a smoothing layer for embedding the concave portion is laminated, Next, a transfer body in which an adhesive layer is laminated can be mentioned.
As another example, a release layer and a protective layer are provided between the transfer substrate 1 and the hologram forming layer 2, and the transfer substrate / release layer / protective layer / hologram forming layer / reflective thin film layer / nucleic acid-containing ink. A transfer body having a layer structure of a layer / adhesive layer can also be used (in the present invention, “/” used between layers means a boundary surface between layers). Here, a layer structure that does not include any one of the peeling layer and the protective layer is also one aspect of the present invention.

<2> Transfer base material The transfer base material used in the transfer body of the present invention serves as a support for laminating a transfer layer to be transferred to the transfer body during the manufacture of the transfer body. The transfer body of the present invention is superimposed on the article to be transferred so that the adhesive layer faces the article surface, and the transfer layer is transferred from the transfer substrate as the support to the article surface, and then printed. Then, the transfer substrate 1 is peeled off and removed.
As the transfer substrate, various materials can be applied depending on the application as long as they have mechanical strength, heat resistance, and solvent resistance that can withstand processing during production. Since it depends on the purpose of use, it is not limited, but a film-like or sheet-like plastic is preferable.
For example, various plastic films such as polyethylene terephthalate (PET), polycarbonate, polyvinyl alcohol, polysulfone, polyethylene, polypropylene, polystyrene, polyarylate, triacetyl cellulose (TAC), diacetyl cellulose, and polyethylene / vinyl alcohol can be exemplified. .
The thickness of the transfer substrate can be appropriately changed depending on the material so that its strength and thermal conductivity are appropriate, but is usually about 2 to 50 μm, preferably from the viewpoint of transferability. 2.
5 to 12 μm. If it is thicker than 50 μm, heat transfer may be deteriorated during transfer printing, and if it is less than 2 μm, the mechanical strength as a substrate tends to be insufficient.

<3> Release layer As a layer constituting a part of the transfer layer transferred from the transfer substrate to the article to be transferred by heating at the time of transfer printing of the transfer body of the present invention, in some cases, directly with the substrate A release layer may be formed as a layer in contact with. By having this release layer, the transfer layer can be reliably and easily transferred from the thermal transfer sheet to the transfer medium. Release layers commonly used in the art can be used, such as microcrystalline wax, carnauba wax, paraffin wax, Fischer-Tropsch wax, various low molecular weight polyethylenes, wood wax, beeswax, whale wax, ibota wax, wool wax. , Shellac wax, candelilla wax, petrolactam, partially modified wax, fatty acid ester, fatty acid amide and other waxes, silicone wax, silicone resin, fluororesin, acrylic resin, polyester resin, polyurethane resin, cellulose resin, vinyl chloride -It can be formed using a thermoplastic resin such as vinyl acetate copolymer or nitrified cotton.

The release layer can be formed from a binder resin and a releasable material. As the binder resin, thermoplastic resins such as polymethyl methacrylate, polyethyl methacrylate, polybutyl acrylate and other acrylic resins, polyvinyl acetate, vinyl chloride-vinyl acetate copolymer, polyvinyl alcohol, polyvinyl butyral, etc. Vinyl-based resins, cellulose derivatives such as ethyl cellulose, nitrocellulose, and cellulose acetate, unsaturated polyester resins that are thermosetting resins, polyester resins, polyurethane resins, amino alkyd resins, and the like can be used. Furthermore, an ionizing radiation curable resin constituting a protective layer and a hologram forming layer, which will be described later, can also be used. As the releasable material, waxes, silicone waxes, silicone resins, melamine resins, fluorine resins, fine powders of talc and silica, lubricants such as surfactants and metal soaps, and the like can be used.
The release layer was prepared by dissolving or dispersing the necessary materials with an appropriate solvent to prepare a release layer coating solution, and using a gravure printing method, screen printing method or gravure plate on the transfer substrate. It can be formed by applying and drying by means such as reverse coating. The thickness after drying is usually 0.1 to 10 g / m ² , but can be adjusted as appropriate. The thickness of the release layer can be appropriately set, but is usually 0.1 to 1 μm from the viewpoint of peelability and transferability.
The degree is appropriate.

<4> Protective layer As a layer constituting a part of the transfer layer provided on the transfer substrate of the transfer body of the present invention, as a layer for directly contacting the hologram forming layer described below and protecting the layer, In some cases, a protective layer may be formed. This contributes to improving the scratch resistance of the hologram forming layer and the durability such as water resistance. This protective layer can be formed of various resins conventionally known as protective layer forming resins. Examples of the resin for forming the protective layer include, as thermoplastic resins, polyester resins, polystyrene resins, acrylic resins, polyurethane resins, acrylic urethane resins, epoxy resins, phenoxy resins, resins obtained by modifying these resins with silicone, these A mixture of these resins, ionizing radiation curable resin, ultraviolet blocking resin and the like can be exemplified. In addition to this, an ultraviolet absorber, an organic filler and / or an inorganic filler can be appropriately added as necessary.

  The protective layer containing the ionizing radiation curable resin is particularly excellent in plasticizer resistance and scratch resistance. As the ionizing radiation curable resin, known ones can be used. For example, a radical polymerizable polymer or oligomer is crosslinked and cured by ionizing radiation irradiation, and a photopolymerization initiator is added if necessary, and an electron beam Those obtained by polymerization and crosslinking with ultraviolet rays can be used. The protective layer containing an ultraviolet blocking resin or an ultraviolet absorber particularly provides light resistance to the nucleic acid-containing ink layer described below, and maintains the function of the contained nucleic acid as a sequence information medium for a long period of time. The main purpose. As the ultraviolet blocking resin, for example, a resin obtained by reacting and bonding a reactive ultraviolet absorber with a thermoplastic resin or the above ionizing radiation curable resin can be used. For example, an addition-polymerizable double bond (for example, a non-reactive organic ultraviolet absorber such as salicylate, phenyl acrylate, benzophenone, benzotriazole, coumarin, triazine, nickel chelate) Examples thereof include vinyl groups, acryloyl groups, methacryloyl groups, etc.), alcoholic hydroxyl groups, amino groups, carboxyl groups, epoxy groups, and isocyanate groups into which reactive groups have been introduced.

  The protective layer is formed by adding the protective layer-forming resin described above and, if necessary, additives such as ultraviolet absorbers, organic fillers and / or inorganic fillers, and dissolving or dispersing them in an appropriate solvent. A resin composition for formation is prepared, and this is applied to the above-mentioned transfer substrate or, if a release layer is provided, on the release layer, for example, a gravure printing method, a screen printing method, a reverse coating method using a gravure plate It can apply | coat by forming means, such as, and can dry and form. The protective layer can be formed to have an arbitrary thickness, but usually about 0.1 to 1 μm is appropriate in terms of protective ability and transferability.

<5> Hologram-forming layer As a layer constituting a part of the transfer layer provided on the transfer substrate of the transfer body of the present invention, or on the release layer when a release layer is provided, and When a protective layer is provided, a hologram forming layer for forming a hologram is provided on the protective layer.
In the transfer body of the present invention, the hologram formed by the hologram forming layer may be any conventionally known hologram or a combination thereof. For example, it may be a surface relief type hologram forming layer with irregularities formed on its surface, and a volume type characterized in that a hologram is formed by recording interference fringes in the forming material It may be a hologram forming layer. In particular, the surface relief hologram is preferable in that the foil breakage is good at the time of transfer printing by heat.

Surface relief type hologram forming layer As a material of the surface relief type hologram forming layer, various resins conventionally used for forming a surface relief type hologram, such as thermosetting resins, thermoplastic resins, ionizing radiation curable resins, etc. Various resin materials can be selected.
Examples of the thermosetting resin include unsaturated polyester resins, acrylic urethane resins, epoxy-modified acrylic resins, epoxy-modified unsaturated polyester resins, alkyd resins, and phenol resins.
Examples of the thermoplastic resin include acrylate resin, acrylamide resin, nitrocellulose resin, and polystyrene resin. These resins are used alone or as a copolymer of two or more. These resins may be used alone or as a mixture of two or more kinds of isocyanate resins, metal soaps such as cobalt naphthenate and zinc naphthenate, benzoyl peroxide, methyl ethyl ketone peroxide, benzophenone, acetophenone, anthraquinone, naphthoquinone. Further, a heat or ultraviolet curing agent such as azobisisobutyronitrile or diphenyl sulfide may be blended.

  Examples of the ionizing radiation curable resin include epoxy acrylate, urethane acrylate, and acrylic-modified polyester. These ionizing radiation curable resins may include other monofunctional or polyfunctional monomers, oligomers, and the like as described below for the purpose of adjusting the cross-linked structure and viscosity. For example, monofunctional (mono) methacrylate such as tetrahydrofurfuryl (meth) acrylate, hydroxyethyl (meth) acrylate, vinylpyrrolidone, (meth) acryloyloxyethyl succinate, (meth) acryloyloxyethyl phthalate, etc. In skeleton structure, polyol (meth) acrylate (epoxy-modified polyol (meth) acrylate, lactone-modified polyol (meth) acrylate, etc.), polyester (meth) acrylate, epoxy (meth) acrylate, urethane (meth) acrylate, etc. Poly (meth) acrylates with polybutadiene-based, isocyanuric acid-based, hydantoin-based, melamine-based, phosphoric acid-based, imide-based, phosphazene-based skeletons, UV and electron beam curable There various monomers, oligomers, polymers may be utilized.

  Further, when forming the surface relief hologram forming layer, a press tamper with irregularities formed on the surface is pressed against the forming material to form irregularities on the surface of the forming material. At this time, the forming material A release agent can be added in advance so that can be easily peeled off from the press tamper. As the release agent to be used, conventionally known release agents, for example, polyethylene wax, amide wax, solid wax such as Teflon (registered trademark) powder, fluorine-based, phosphate-based surfactant, silicone and the like are all used. It can be used. Particularly preferable release agent is modified silicone, specifically, modified silicone oil side chain type, modified silicone oil both end type, modified silicone oil one end type, modified silicone oil side chain both end type, trimethylsiloxysilicate. And polymethylsiloxane (referred to as silicone resin), silicone graft acrylic resin, and methylphenyl silicone oil.

In order to form a hologram forming layer using the above resin material, it can be directly formed by developing the photosensitive resin material by performing interference exposure of the hologram. It is preferable to perform molding by using a replica or a plating mold thereof as a replica mold and pressing the mold surface against the layer of the resin material.
When thermosetting resin or ionizing radiation curable resin is used, curing is performed by heating or ionizing radiation irradiation while keeping the uncured resin in close contact with the mold surface, and then cured by peeling after curing. The fine irregularities of the relief hologram can be formed on one side of the layer made of a transparent resin material. A diffraction grating forming layer having a diffraction grating formed in a pattern by a similar method can also be used as the optical diffraction structure.
The thickness of the surface relief type hologram forming layer is usually 0.1 to 5 μm, particularly about 0.1 to 3 μm, but the thickness is not limited as long as the hologram can be formed, and can be adjusted as appropriate.

Volume hologram forming layer As a material for the volume hologram forming layer, various materials conventionally used for volume hologram formation can be selected. Silver salt materials, dichromated gelatin emulsions, photopolymerizable resins, Examples include known volume hologram recording materials such as photocrosslinkable resins, and in particular, photosensitive materials for dry volume phase hologram recording applications, including matrix polymers, photopolymerizable compounds, photopolymerization initiators, and sensitizing dyes. The thing which consists of is mentioned.
Examples of the photopolymerizable compound include photopolymerization having at least one ethylenically unsaturated bond in one molecule as described later, photocrosslinkable monomers, oligomers, prepolymers, and mixtures thereof. Examples thereof include unsaturated carboxylic acids and salts thereof, esters of unsaturated carboxylic acids and aliphatic polyhydric alcohol compounds, and amide bonds of unsaturated carboxylic acids and aliphatic polyvalent amine compounds.
As photopolymerization initiators, 1,3-di (t-butyldioxycarbonyl) benzophenone, 3,3 ′, 4,4′-tetrakis (t-butyldioxycarbonyl) benzophenone, 2,4,6-tris (Trichloromethyl) -s-triazine, 2-mercaptobenzimidazole, imidazole dimers and the like are exemplified.
Examples of the sensitizing dye include thiopyrylium salts, merocyanines, quinolines, ketocoumarins, thioxanthenes, cyanines, rhodamines, diphenyliodonium salts and the like.

  Examples of matrix polymers include polymethacrylates or partial hydrolysates thereof, polyvinyl acetate or hydrolysates thereof, polyvinyl alcohol or partial acetal compounds thereof, triacetyl cellulose, polyvinyl butyral, polyvinyl chloride, and poly-N-vinylcarbazole. Or a derivative thereof, poly-N-vinylpyrrolidone or a derivative thereof, a copolymer of styrene and maleic anhydride, acrylic acid, acrylic acid ester, methacrylic acid, methacrylic acid ester, acrylamide, acrylonitrile, vinyl acetate and the like can be copolymerized A copolymer having at least one monomer group as a polymerization component or a mixture thereof is used.

The photopolymerizable compound is used in a ratio of 10 to 1000 parts by weight, preferably 10 to 100 parts by weight, based on 100 parts by weight of the binder resin. A photoinitiator is used in the ratio of 1-10 weight part with respect to 100 weight part of binder resin, Preferably it is 5-10 weight part. The sensitizing dye is used in a proportion of 0.01 to 1 part by weight, preferably 0.01 to 0.5 part by weight, based on 100 parts by weight of the binder resin.
The thickness of the volume hologram forming layer is usually about 1 to 100 μm, particularly about 2 to 40 μm, but the thickness is not limited as long as the hologram can be formed, and can be appropriately adjusted.

<6> Reflective thin film layer In the transfer body of the present invention, in order to enhance the reflection and / or diffraction effect of the hologram on the hologram forming layer provided on the transfer substrate, the release layer, or the protective layer as described above. A reflective thin film layer is formed on a part or the whole surface. As a formation method, a known method such as sublimation, vapor deposition, CVD (chemical vapor deposition), sputtering, reactive sputtering, ion plating, electroplating or the like can be used.
As the reflective thin film layer, any film can be used as long as it can exhibit the hologram effect. For example, a metallic glossy thin film layer such as a metal thin film by a vacuum thin film method or a transparent reflective thin film layer can be used. Either is acceptable.

The transparent reflective thin film layer has a nearly colorless and transparent hue, and its optical refractive index is different from that of the hologram forming layer, so that it is possible to visually recognize the glitter of a hologram or the like even though there is no metallic luster. A transparent hologram can be produced. For example, there are a thin film having a higher refractive index than the hologram forming layer and a thin film having a lower refractive index. Examples of the former include ZnS, TiO ₂ , Al ₂ O ₃ , Sb ₂ S ₃ , SiO, and SnO _2. ITO, etc., and examples of the latter include LiF, MgF ₂ , and AlF ₃ . Preferably, it is a metal oxide or nitride, specifically, Be, Mg, Ca, Cr, Mn, Cu, Ag, Al, Sn, In, Te, Fe, Co, Zn, Ge, Pb, Cd , Bi, Se, Ga, Rb, Sb, Pb, Ni, Sr, Ba, La, Ce, Au, and other oxides or nitrides, and the like can be exemplified by a mixture of two or more thereof. Also, a general light-reflective metal thin film such as aluminum can be used when it has a thickness of 200 mm or less. The transparent metal compound may be formed on the hologram forming layer so as to have a thickness of about 10 to 2000 nm, preferably 20 to 1000 nm, as with the metal thin film.

<7> Nucleic acid-containing ink layer In the transfer body of the present invention, a nucleic acid-containing ink layer is partially provided on the reflective thin film layer. In the present invention, the nucleic acid-containing ink layer is a layer made of a nucleic acid-containing ink composition containing a nucleic acid and a fluorescent dye.

Nucleic acid In the present invention, a nucleic acid is DNA (including nuclear DNA and extranuclear DNA), RNA, and derivatives thereof, which may be naturally derived or artificial, and constitute the nucleic acid. Nucleotides may be naturally occurring or artificially produced. Further, the nucleic acid may be in either a single stranded fragment or a double stranded fragment, and its length is an oligomer of less than about 100 bases / base pair, depending on the authentication application. Or a polymer composed of more nucleotides. For the purpose of individual identification, it is preferable to use a nucleic acid fragment having a length of preferably 10 bases / base pair or more, more preferably 100 bases / base pair or more. In addition, from the viewpoint of handling during production and detection, storage stability, ease of degradation, etc., the length of each fragment is 1000 bases / base pair or less, more suitably 500 bases / base pair or less. It is preferable to synthesize them as described above, or cleave with restriction enzymes.

In the present invention, DNA obtained by pouring DNA from a living body, for example, a human cell, and decomposing it with, for example, a restriction enzyme may be mixed with other components of the nucleic acid-containing ink composition. In addition, a specific sequence, preferably a specific polymorphic region, preferably a STR sequence can be amplified by PCR and used. Furthermore, DNA or RNA derived from non-human animals and plant cells can also be used. On the other hand, a nucleic acid synthesized by a conventionally known chemical synthesis method may be used.
The nucleic acid obtained as described above may consist of only a fragment having one type of sequence or a mixture of fragments having different sequences, as necessary.
In the present invention, the nucleic acid is blended at a ratio of 1 × 10 ⁻² to 1 × 10 ⁵ μg, preferably 1 to 1 × 10 ⁴ μg, based on 100 g of the solid content in the nucleic acid-containing ink composition. Is preferred. It is essential that one or more nucleic acids are contained in the nucleic acid-containing ink layer. When the amount is less than 1 × 10 ⁻² μg, a specific site provided with the nucleic acid-containing ink layer is cut out from the transfer body, and the nucleic acid is removed. It can be difficult to collect and sequence. On the other hand, when the amount is more than 1 × 10 ⁵ μg, it is difficult to match the refractive index of the adhesive layer or the smoothing layer surrounding the nucleic acid-containing ink layer, and it is easy to visually recognize from the outside, which is not preferable. In addition, the probability of contamination in which nucleic acids are mixed outside the target composition during production is also increased.

Fluorescent dyes In the nucleic acid-containing ink composition constituting the nucleic acid-containing ink layer, various fluorescent dyes that are conventionally used may be used as the fluorescent dye.
Specifically, organic fluorescent dyes and inorganic fluorescent dyes can be used. Examples of organic fluorescent dyes include diaminostilbene disulfonic acid derivatives, imidazole derivatives, coumarin derivatives, triazoles, carbazoles, pyridine, naphthalic acid, imidazolone derivatives, fluorescein, etc. And dyes such as eosin, and compounds having a benzene ring such as anthracene. In addition, examples of the inorganic fluorescent dye include, for example, oxides such as Ca, Ba, Mg, and Sr, crystals such as sulfides, silicates, phosphates, and tungstates as main components, and Eu, Mn, and Pb. , Fe, Mn, Zn, Ag, Cu and other metal elements or rare earth elements can be used as a dopant.

Further, when the nucleic acid is double-stranded, a fluorescent dye that enters into the double helix structure and forms a double-stranded nucleic acid-fluorescent dye complex can be preferably used. In the nucleic acid-containing ink composition of the present invention, when such a combination of a fluorescent dye and a double-stranded nucleic acid is used, the electron cloud of the fluorescent dye has a strong interaction with an electron cloud of a base pair having a double helix structure. Demonstrates that the double helix structure is physically strong, and the resistance to degradation and stability are increased. Such fluorescent dyes do not have a three-dimensional structure, but have a two-dimensional planar structure, which is about 0.
A skeleton having a size of about 4 nm × 1.0 to 1.4 nm and having three benzene rings connected (anthracene skeleton, phenanthrene skeleton), or having one phenyl group connected to two benzene rings as a substituent. To do. Specifically, anthracene skeleton, phenanthrene skeleton, dibenzofuran skeleton, anthraquinone skeleton, indigo, thioindigo skeleton, diphenylmethane skeleton, triphenylmethane skeleton, xanthene skeleton, anthocyanin skeleton, fluorescein skeleton, rhodamine skeleton, thiazine skeleton, benzidine skeleton, ditrizine Examples include skeletons, dianisidine skeletons, diaminoacridine skeletons and the like in which auxiliary color groups are substituted. These fluorescent dyes have a carbocyclic or heterocyclic skeleton in one plane, and an electron cloud composed of π electron linkages on the plane above and below the plane. Are at a distance of about 0.2 nm and express interaction, and both ends of the fluorescent dye interact with oxygen in the phosphate / ribosome structure constituting the pillar of the DNA helical structure. One benzene ring (phenyl group) is added to the phenanthrene skeleton, and two amino groups as an auxiliary color group, ethidium bromide, acridine orange, cyber green I and II, and a rare earth complex as a substituent to the naphthalene diimide skeleton The fluorescent dye is a typical fluorescent dye capable of forming the complex, and can be preferably used because of its physical stability.

In the present invention, the nucleic acid-containing ink composition contains a combination of double-stranded DNA and a fluorescent dye that can enter a double helix structure to form a complex from the viewpoint of physical stability. It is preferable. Such a DNA-containing ink composition exhibits excellent structural stability over an extremely long period of time under the condition that the transfer material of the present invention is coated with the resin composition of the adhesive layer or the smoothing layer, and is excellent. And storage stability such as light resistance and weather resistance.
Furthermore, the fluorescent dye that forms a complex by entering into the double helix structure is affected by the non-covalent interaction, and the energy gap between the excited state and the ground state emitting fluorescence is increased. It is narrowed and a long wavelength shift of the fluorescence wavelength appears. For example, acridine orange normally emits fluorescence at 650 nm with respect to excitation light of 460 nm. However, when entering a double helical structure to form a complex, acridine orange emits fluorescence at 526 nm with respect to excitation light of 500 nm. . By strictly managing this wavelength shift, for example, a specific fluorescence wavelength is emitted by irradiating a specific excitation wavelength to a specific part without performing a destructive inspection by cutting out a transfer layer and collecting a nucleic acid. Whether or not non-destructive inspection is possible.

  In the present invention, the fluorescent dye is preferably blended at a ratio of 0.001 to 1000 μg, preferably 0.01 to 100 μg, based on 100 g of the solid content in the nucleic acid-containing ink composition. If it is less than 0.001 μg, the reliability in determining authenticity becomes insufficient, and if it is more than 1000 μg, the proportion of the fluorescent dye not incorporated in the nucleic acid increases and its stability decreases. At the same time, the presence of the fluorescent dye itself tends to be exposed, which is not preferable.

Nucleic acid-containing ink composition The above-described nucleic acid and fluorescent dye can be inked by a conventionally known method. For example, a nucleic acid and a fluorescent dye can be appropriately mixed with a solvent and other components such as a vehicle and various additives to form an ink composition.
As the solvent, various solvents used for dissolving or suspending nucleic acids can be appropriately used. For example, sterilized water, alcohol (for example, methanol, ethanol, propanol, butanol, pentanol, hexanol, heptanol) , Octanol, nonanol, etc.), esters (eg, ethyl acetate, butyl acetate, propyl acetate, etc.), ketones (eg, acetone, dimethyl ketone, methyl ethyl ketone, diethyl ketone, etc.) and aromatic solvents (toluene, hexane, cyclohexane, xylene, etc.), And mixtures thereof, but are not limited thereto.

As the vehicle, a resin generally used in an ink composition can be appropriately used. For example, polymethyl methacrylate (refractive index n = 1.49), polymethyl acrylate (n = 1.47), poly Hexidyl methacrylate (n = 1.54), polybenzyl methacrylate (n = 1.57), polybutyl acrylate (n = 1.44), polyisobutyl acrylate (n = 1.48), cellulose nitrate resin (n = 1.54), methylcellulose resin (n = 1.50), cellulose resin (n = 1.54), cellulose acetate propionate resin (n = 1.47), polystyrene (n = 1.60), polyethylene Terephthalate (n = 1.64), polyvinyl acetate (n = 1.47), polyvinyl chloride / vinyl acetate (n = 1.54), melamine resin n = 1.56), epoxy resin (n = 1.61),
A phenol resin (n = 1.60) or a mixture thereof can be used. In the present invention, these resins are used so that the difference between the refractive index of the nucleic acid-containing ink layer and the refractive index of the adhesive layer or smoothing layer described below is 0.1 or less, preferably 0.03 or less. And a resin mixture is selected. As one of the preferable embodiments, there is a case where the refractive index difference can be reduced by using the same resin composition as the resin composition forming the adhesive layer and / or the smoothing layer.

Various known additives such as a fluidity adjusting agent, a drying adjusting agent, and a processing adjusting agent may be further added to the mixture containing the nucleic acid, the solvent and the vehicle.
In addition, the nucleic acid may be appropriately mixed with the vehicle and other components after being subjected to a surfactant treatment, a moisturizing treatment with hyaluronic acid or the like, or a freeze drying treatment. In particular, dispersibility in the vehicle is enhanced by treatment with a surfactant. In addition, when subjected to freeze-drying treatment, particularly when the nucleic acid is a double-stranded DNA, the double helix structure is preferably maintained, and the fluorescent dye is incorporated therein, so that light resistance and weather resistance are improved. Increases further.

Layer formation In the transfer body of the present invention, the nucleic acid-containing ink composition obtained as described above can be appropriately printed and formed at an arbitrary thickness on an arbitrary portion on the reflective thin film layer. For printing, for example, a gravure coating method, an offset printing method, a letterpress printing method, a screen printing method, a curtain coating method, an ink jet method, or the like can be used.
The transfer body of the present invention requires remarkably higher reliability than the conventional anti-counterfeit technology, and the effect is further enhanced by bringing the concentration of nucleic acid contained in the transfer body close to the detection limit. Therefore, it is important to prevent even slight contamination of nucleic acids in order to eliminate false detection as much as possible and perform precise detection. Therefore, the screen printing method, which can batch-process printing substrates, printing plates, inks, etc., prevents nucleic acid contamination and accurately spots the target position on the reflective thin film layer. Since printing can be performed, it can be particularly suitably used in the present invention. In particular, when the adhesion of the nucleic acid-containing ink composition to be used due to airborne droplets is confirmed by fluorescence detection or the like, a stainless screen printing method with high detection accuracy can be suitably used. Further, when screen printing is performed, it is easy to isolate the periphery of the printing area of the screen plate by making the ink supply unit completely sealed. In particular, it is desirable that the printing area of the nucleic acid-containing ink layer of the present invention has a small area, and it is possible to print in one place with a size of about 10 μm ² in the screen printing method.

The nucleic acid-containing ink layer can be formed with an appropriate thickness depending on the size and concentration of the nucleic acid contained, but from the viewpoint of detection suitability and concealability, 0.1 to 5 μm, particularly 0.1. It is preferable to form with a thickness of ˜2 μm. Similarly, it is preferable to form in an area of 10 to 5 × 10 ⁸ μm ² , more preferably 100 to 5 × 10 ⁶ μm ² . At this time, the size, concentration, formation area, and formation of the nucleic acid are such that 10 ¹² to 10 ²⁰ , preferably 10 ¹² to 10 ¹⁵ nucleic acid fragments are present per 1 cm ³ occupied area of the nucleic acid-containing ink layer. It is preferable to adjust the thickness.

<8> Adhesive layer In the transfer body of the present invention, an adhesive layer is provided after the nucleic acid-containing ink layer is partially provided on the reflective thin film layer.
The adhesive layer is not particularly limited as long as it is a heat-sensitive or pressure-sensitive adhesive that does not have adhesiveness at normal temperature or normal pressure, and exhibits an adhesive force only when heated or pressurized. For example, rubber systems such as polyisoprene rubber, polyisobutylene rubber, styrene butadiene rubber; poly (meth) methyl acrylate, poly (meth) ethyl acrylate, poly (meth) acrylate, poly (meth) acrylate, (Meth) acrylic acid esters such as poly (meth) acrylic acid-2-ethylhexyl; polyvinyl ethers such as polyisobutyl ether; polyvinyl chlorides such as polyvinyl acetate and vinyl chloride-vinyl acetate copolymer; polyacrylamide Polyamides such as polymethylolacrylamide; Vinyl chlorides such as polyvinyl chloride; Other vinyls such as polyvinyl butyral, vinyl acetate / octyl acrylate, vinyl acetate / butyl acrylate, vinylidene chloride / butyl acrylate; polystyrene, etc. An aromatic vinyl system of; and Li chloride olefin and the like, can be used alone or in combination of two or more select only one among these. However, the refractive index as the adhesive layer is different from the refractive index of the adjacent diffusion-containing ink layer, and if there is a smoothing layer, the difference between the refractive index of the smoothing layer is 0.1 or less, especially 0. It is necessary to select the resin that constitutes the adhesive composition so as to be 0.03 or less. In addition, when used as a transfer foil or transfer ribbon, it is necessary to make a selection so as to ensure sufficient adhesion with the reflective thin film layer.

The resin as described above may be melted by heat to form a liquid, which may be applied and cooled by a roll coating method, a comma coating method, a hot malt applicator method, or the like.
Although the thickness of an adhesive bond layer can be set suitably, Preferably it is 1-5 micrometers, Most preferably, it is 2-4 micrometers. If it is 1 μm or less, sufficient adhesiveness may not be obtained, and if it is 5 μm or more, foil breakage may be poor during heating or pressurization.
You may add various additives, such as a white pigment, an extender pigment, and a fluorescent whitening agent, to the adhesive composition which comprises the said adhesive bond layer as needed.
In addition to the heat-sensitive or pressure-sensitive adhesive, it is possible to use various arbitrary pressure-sensitive adhesives. In that case, however, the release film that is peeled off when applied to the article surface is applied to the layer surface. Used by sticking.

<9> Smoothing layer In the transfer body of the present invention, the nucleic acid-containing ink layer is partially provided on the reflective thin film layer, and then, if necessary, the portion provided with the nucleic acid-containing ink layer is not provided. In order to smooth the unevenness on the reflective thin film layer composed of the portion, a smoothing layer for embedding the concave portion may be laminated, and then the adhesive layer may be laminated.
As the smoothing layer, any resin or resin composition can be used as long as it is applied for the purpose of flattening the unevenness, provided that the refractive index of the smoothing layer and the nucleic acid-containing ink layer It is necessary to select the resin so that the difference from the refractive index is 0.1 or less, preferably 0.03 or less. The same resin composition as that used in the hologram forming layer, nucleic acid-containing ink layer and adhesive layer can be used, and preferably the same composition as the resin composition forming the adhesive layer is used. Also good.

The smoothing layer can be formed by applying, drying, and curing by a known coating method such as a roll coating method, a Miya bar coating method, or a gravure coating method using a liquid resin composition or the like. The thickness of the smoothing layer can be determined as appropriate according to the thickness of the nucleic acid-containing ink layer.
By providing the smoothing layer before providing the adhesive layer, the smoothness of the finally obtained adhesive layer is increased, and a uniform surface without unevenness is obtained. As a result, heat and pressure are uniformly applied during transfer printing, and transfer quality is improved. For example, when the article to be transferred is not a porous polymer material such as a paper substrate, but is a card substrate or the like and is difficult to absorb the adhesive resin, the smoothing layer is one or two. By providing more than one layer, the concealability can be improved by reducing the accessibility to the nucleic acid-containing ink layer.

<10> Physical stability In the field of anti-counterfeiting as in the present invention, a transfer body is required to have a stable authenticity assurance capability of usually 1 year or longer, and further 5 years or longer. By having the structure of the present invention, even a nucleic acid that is relatively easily degradable can exhibit weather resistance, light resistance, and physical stability that can withstand the above requirements. In addition, each layer constituting the transfer layer, that is, at least one of a peeling layer, a protective layer, a hologram forming layer, a reflective thin film layer, a nucleic acid-containing ink layer, a smoothing layer, and an adhesive layer is provided with an ultraviolet absorber. By containing, deterioration over time due to ultraviolet rays or the like can be suppressed, the physical stability of the contained nucleic acid can be further increased, and sequence information can be preserved for a long period of time.

  The said ultraviolet absorber is a conventionally well-known non-reactive organic type ultraviolet absorber, and salicylate type, phenyl acrylate type, benzophenone type, benzotriazole type, coumarin type, triazine type and nickel chelate type are mentioned. Specific examples of the organic filler and / or inorganic filler include polyethylene wax, bisamide, nylon, acrylic resin, cross-linked polystyrene, silicone resin, silicone rubber, talc, calcium carbonate, titanium oxide, microsilica, colloidal silica, and other silica fine particles. Although powder etc. are mentioned, it does not specifically limit and anything can be used. The ultraviolet absorber can be appropriately added as long as the transparency of the layer is maintained, and preferably a ratio of 0.01 to 0.05 g with respect to 100 g of the solid content in the composition constituting each layer. It is blended with.

<11> Detection In the above, since the nucleic acid-containing ink layer is preferably provided in a small area and is difficult to be visually recognized from the outside, inconvenience may occur not only for those who try to counterfeit but also for legitimate detectors. Therefore, in order to eliminate such inconvenience, at least one layer constituting the transfer layer may be provided with one or more detection marks for detecting the position of the portion where the nucleic acid-containing ink layer is provided. Good. As a result, a legitimate detector aligns the article to which the transfer layer from the transfer body of the present invention is applied with reference to the detection mark, and fluorescence is detected in the portion where the nucleic acid-containing ink layer is provided. And whether the fluorescence wavelength is authentic, and whether the nucleic acid contained in the part has valid sequence information can be examined.

Such a detection mark may be a visible print, a cutout, a part of a hologram image, or the like, or cannot be visually recognized under a normal environment like a laser reproduction hologram. Also good.
Furthermore, in order to counter the leakage of information and prevent unauthorized acquisition of nucleic acids, an ink composition that contains only fluorescent dyes and does not contain nucleic acids is provided in one or more parts of the transfer body to complicate detection. Also good.

The present invention will be described in more detail by the following examples, but is not limited thereto.
Example 1
The surface of a PET film having a thickness of 12 μm as a transfer substrate was coated with a thermosetting resin composition made of a melamine resin (manufactured by The Inktec Co., Ltd., Iupimer LZ). A surface relief hologram is formed by bringing a surface relief hologram replication mold having a detection pattern (a diffraction grating of 1000 μm × 500 μm size) into contact with the mold and subjecting it to a heat curing treatment, and a hologram forming layer having a thickness of 3 μm is formed. Obtained.
Subsequently, ZnS was vacuum-deposited on the surface on which the relief hologram was formed, thereby forming a reflective transparent thin film layer having a thickness of 50 nm.

  On the other hand, DNA derived from salmon mushroom in 74 μl of sterilized water (manufactured by Nichiro Co., Ltd., DNA solution extracted from salmon mushroom, fragmented to 300 bp or less), 1.3 mg (4000 nmol base), and 3 μl of sterilized water A solution of 20 nmol of fluorescent dye (acridine orange) (1% / base pair) and a solution of 6000 nmol of alkyl lipid (C18 lipid) (1.5 times the base) in 24 μl of sterilized water, These were mixed and the precipitate was collected to remove excess alkyl lipid, washed, freeze-dried, and then 28 mg of a surfactant-treated DNA / fluorescent dye complex was obtained. 1 mg of the obtained complex was dissolved in 50 μl of ethanol and mixed with 10 g of cellulose resin / acrylic resin (2: 1) and 1 g of ethanol / ethyl acetate (2: 1) to obtain a nucleic acid-containing ink composition.

The obtained nucleic acid-containing ink composition is silk-screen printed at a predetermined position on the reflective thin film layer while detecting an image position detection pattern, dried at 50 ° C. for 10 minutes, and then contains a nucleic acid having a size of 200 μm × 200 μm. An ink layer (thickness 2 μm, refractive index n = 1.55) was formed.
An adhesive composition having the following composition was applied thereon and dried at 70 ° C. for 10 minutes to form an adhesive layer (thickness 3 μm, refractive index n = 1.56). Obtained.
<Adhesive composition>
・ Cellulose resin / acrylic resin (2: 1) (manufactured by Seiko Advance Co., Ltd., STR T475) 30 parts by mass ・ External pigment (calcium carbonate) 5 parts by mass ・ Methyl isobutyl ketone 25 parts by mass ・ Ethyl acetate 40 parts by mass

  When the transfer body of the present invention obtained above was superposed so that the adhesive layer and the surface of the transfer target face each other and transferred with a thermal printer, a good hologram reproduction image was confirmed by visual observation. Next, irradiation with an excitation wavelength of 500 nm was performed, and fluorescence at 526 nm was confirmed from a predetermined position. Furthermore, the said part was cut out, DNA was melt | dissolved and extracted, the PCR amplification process was carried out, the base sequence of the contained DNA fragment was read with the DNA sequencer, and it was confirmed that it was DNA of the target salmon egg white origin.

Separately, in accordance with JIS L0843, an exposure test was conducted with a xenon arc lamp at an irradiance of 0.35 W / m ² (340 nm) based on a dyeing fastness test method for a xenon arc lamp. After exposure for 100 hours, the DNA-containing part was cut out in the same manner as described above, the DNA was dissolved and extracted, subjected to PCR amplification, and the nucleotide sequence of the contained DNA fragment was read with a DNA sequencer. Was confirmed.

(Example 2)
As a fluorescent dye in the nucleic acid-containing ink composition, pyronine 20 nmol (1% / base pair) / 9 μl sterilized water was used instead of acridine orange, and the transfer body of the present invention was obtained in the same manner as in Example 1. Transfer printed on the body surface.
A good hologram reproduction image was confirmed by visual observation, and when an excitation wavelength of 564 nm was irradiated, fluorescence at 576 nm was confirmed from a predetermined position. Furthermore, the said part was cut out, DNA was melt | dissolved and extracted, the PCR amplification process was carried out, the base sequence of the contained DNA fragment was read with the DNA sequencer, and it was confirmed that it was salmon egg white origin DNA. Further, when an exposure test was performed with a xenon arc lamp in the same manner as in Example 1, the target base sequence could be read even after 100 hours of exposure.

(Example 3)
In the same manner as in Example 1, a hologram forming layer and a reflective thin film layer were formed on a transfer substrate, and the prepared nucleic acid-containing ink composition was silk-screen printed at a predetermined position on the reflective thin film layer. After drying at 50 ° C. for 10 minutes, a nucleic acid-containing ink layer (thickness 2 μm, refractive index n = 1.55) having a size of 1000 μm × 1000 μm was formed.

Next, a resin composition having the following composition was applied to a portion where the nucleic acid-containing ink layer was not formed, and a smoothing layer (thickness 2 μm, refractive index n = 1.56) was formed to obtain a smooth surface.
<Resin composition>
・ Cellulose resin / acrylic resin (2: 1) (manufactured by Seiko Advance Co., Ltd., STR T475) 30 parts by mass ・ External pigment (calcium carbonate) 5 parts by mass ・ Methyl isobutyl ketone 25 parts by mass ・ Ethyl acetate 40 parts by mass

On the smooth surface obtained above, an adhesive composition having the following composition was applied and dried at 70 ° C. for 10 minutes to form an adhesive layer (thickness 1 μm, refractive index n = 1.56). The transfer body of the present invention was obtained.
<Adhesive composition>
・ Cellulose resin / acrylic resin (2: 1) (manufactured by Seiko Advance Co., Ltd., STR T475) 30 parts by mass ・ External pigment (calcium carbonate) 5 parts by mass ・ Isopropyl alcohol 40 parts by mass ・ Ethyl acetate 25 parts by mass

  When the transfer body of the present invention obtained above was superposed so that the adhesive layer and the surface of the transfer target face each other and transferred with a thermal printer, a good hologram reproduction image was confirmed by visual observation. Next, irradiation with an excitation wavelength of 500 nm was performed, and fluorescence at 526 nm was confirmed from a predetermined position. Furthermore, the said part was cut out, DNA was melt | dissolved and extracted, the PCR amplification process was carried out, the base sequence of the contained DNA fragment was read with the DNA sequencer, and it was confirmed that it was salmon egg white origin DNA. Further, when an exposure test was performed with a xenon arc lamp in the same manner as in Example 1, the target base sequence could be read even after 100 hours of exposure.

Example 4
In the same manner as in Example 1, a transfer body of the present invention was obtained. However, when forming a surface relief hologram, a hologram for forming a circular reconstructed image having a diameter of 1 mm was formed at a predetermined position by laser light interferometry, and a detection mark was provided.
In the same manner as in Example 1, transfer printing was performed on a transfer medium, and a hologram reproduction image, fluorescence, and DNA sequence were confirmed well. The detection mark was not confirmed by visual inspection.

(Example 5)
A protective layer-forming resin composition having the following composition is prepared, applied on a transfer substrate, and dried to provide a protective layer (thickness: 1.0 μm), and a hologram forming layer is formed thereon. Thus, a transfer body of the present invention was obtained in the same manner as in Example 1 except that a protective layer was provided between the transfer substrate and the hologram forming layer.
<Resin composition for forming protective layer>
・ Acrylic resin (Kuraray Co., Ltd., Parapet HR-S) 10 parts by mass ・ Ultraviolet absorber (Ciba Japan Co., Ltd., Tinuvin PS) 0.1 parts by mass ・ Methyl ethyl ketone 10 parts by mass ・ Microsilica 0.1 parts by mass In the same manner as in Example 1, transfer printing was performed on a transfer medium, and a hologram reproduction image, fluorescence, and DNA sequence were confirmed well. Further, when an exposure test was performed with a xenon arc lamp in the same manner as in Example 1, the target base sequence could be read even after 100 hours of exposure.

(Example 6)
In the same manner as in Example 1, a transfer body of the present invention was obtained. However, when the nucleic acid-containing ink layer is partially provided, a nucleic acid-free ink composition having the following composition is printed at another position on the reflective thin film layer, and the nucleic acid-free ink layer having a size of 200 μm × 200 μm is printed. (Thickness 2 μm, refractive index n = 1.55) was formed.
<Nucleic acid-free ink composition>
・ Fluorescent dye (acridine orange) 20 nmol / 3 μl sterilized water ・ Cellulose resin / acrylic resin (2: 1) (STR T475, manufactured by Seiko Advance Co., Ltd.) 10 g
・ Ethanol / ethyl acetate (2: 1) 1g
In the same manner as in Example 1, transfer printing was performed on a transfer medium, and a hologram reproduction image, fluorescence, and DNA sequence were confirmed well. From the portion where the nucleic acid-free ink layer was provided, no fluorescence was observed for irradiation with excitation light of 500 nm. Instead, fluorescence at 650 nm was observed for 460 nm excitation light irradiation.

1. 1. Transfer substrate 2. Hologram forming layer 3. Reflective thin film layer 4. Adhesive layer Nucleic acid-containing ink layer

Claims (6)

On the transfer substrate, at least a hologram forming layer, a reflective thin film layer, and a nucleic acid-containing ink layer are laminated in this order, and then unevenness on the reflective thin film layer comprising a portion provided with a nucleic acid-containing ink layer and a portion not provided In order to smooth the surface, a smoothing layer for embedding the concave portion is laminated, and further, an adhesive layer is laminated in order,
The nucleic acid-containing ink layer is composed of a nucleic acid-containing ink composition containing a nucleic acid and a fluorescent dye. The nucleic acid-containing ink layer is partially provided on the reflective thin film layer, and emits fluorescence emitted from the fluorescent dye when irradiated with excitation light. The above-mentioned transfer body, which is a layer whose presence can be confirmed by detection .   The transfer body according to claim 1, wherein the difference between the refractive index of the nucleic acid-containing ink layer and the refractive index of the adhesive layer is 0.1 or less.   The transfer body according to claim 1 or 2, wherein the adhesive layer and the smoothing layer are composed of the same resin composition.   The transfer body according to any one of claims 1 to 3, further comprising a release layer between the transfer substrate and the hologram forming layer.   The transfer body according to any one of claims 1 to 4, wherein a protective layer is further provided on a surface of the hologram forming layer opposite to the side where the reflective thin film layer is located.   The transfer body according to any one of claims 1 to 5, wherein a detection mark for detecting a position of a portion where the nucleic acid-containing ink layer is provided is provided on the at least one layer.

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