JP5593987B2 - Inspection device - Google Patents

Description

  The present invention relates to an inspection apparatus for determining the authenticity of an authenticity determination medium used by being affixed to an article such as a credit card in the field of forgery prevention, and more specifically, forgery is difficult. The present invention relates to an inspection apparatus for determining the authenticity of an authenticity determination medium that has a highly reliable authentication information medium and has a high ability to guarantee the authenticity of the article.

  The main use of the authenticity determination medium that is an inspection target of the inspection apparatus of the present invention is a transfer foil, a transfer ribbon, a hologram sheet, or the like used in the forgery prevention field, specifically, a credit card or the like. If the card is counterfeited and used, it may damage the cardholder or card company, etc., driver's license, employee ID card, ID card such as membership card, entrance examination card, passport, banknote, gift certificate , Point cards, stock certificates, securities, lottery tickets, horse tickets, passbooks, boarding tickets, pass tickets, air tickets, admission tickets for various events, play tickets, prepaid cards for transportation and various telephones. That is, the authenticity determination medium is an information recording medium that holds information having economic or social value, information such as identification, and the authenticity of the recording medium itself for the purpose of preventing damage caused by forgery. The present invention is applied to inspection of an article desired to have a function capable of identifying the

In addition to the uses described above, expensive products such as luxury watches, luxury leather products, precious metal products, jewelry, etc., often referred to as luxury brand products, or storage boxes or cases for these expensive products Also, famous brands of mass-produced products, such as audio products, electrical appliances, etc., or tags hung on them, and storage media on which music software, video software, computer software, game software, etc. that are copyrighted works are recorded, Or it can apply also to those cases.
In addition, for products that have the ability to identify their authenticity for the purpose of preventing damage caused by counterfeiting, such as printer toner and paper, such as products whose replacement equipment is limited to genuine materials. Can be applied.
In addition, those with very high social value that require higher reliability, such as paintings and sculptures by famous creators, antiques and jewelry that are recognized worldwide, 100 Agricultural products that are very difficult to re-produce, such as wines that have been aged for nearly a year, and more expensive because there are only a few genuine ones in the world, or only one genuine one in the world. It can be suitably applied to non-existent documents (contracts, wills, etc.).

Conventionally, for the purpose of preventing forgery of the article, a hologram that is said to be difficult to manufacture due to the precision of its structure is often applied as one that can be identified as authentic (for example, Patent Document 1).
In addition, as a means for mechanical determination rather than just visual determination, a medium combining special printing and a fluorescent dye is prepared, and the fluorescence generated when specific excitation light is irradiated to the medium. There is also a device that mechanically detects (see, for example, Patent Document 2).
However, since the hologram manufacturing method itself is known and can be precisely processed by that method, when the hologram is merely for visual judgment, there is no difference between a genuine hologram and a forged hologram. It is difficult to distinguish. The same applies to a printed matter combined with a fluorescent dye, and it is necessary to identify the authenticity of the object using a new authenticity identification method.

JP-A-5-155199 JP 2002-274000 A

An object of the present invention is to provide an inspection apparatus that solves the above-described problems, is difficult to counterfeit, has a highly reliable authentication information medium, and has a high ability to guarantee the authenticity of the article.

  As a result of various studies, the present inventor has found that the above object can be achieved by detecting light emitted from a nucleic acid-containing ink composition containing a nucleic acid and a fluorescent dye under special conditions.

  A first aspect of the present invention is an inspection device (20) for an inspection object that includes at least a portion of a nucleic acid-containing ink layer containing a nucleic acid and a fluorescent dye, wherein the inspection object is set at a predetermined position. Excited by a body mounting table (25), a light source (21) that irradiates the test object with a plurality of different wavelengths of excitation light, and an excitation light (P1) of a predetermined wavelength range that irradiates the test object An optical filter (23) that passes at least part of the emitted light (P2), a detection unit (22) that detects light that has passed through the optical filter, the light source (21), the optical filter (23), and a detection A light having a specific wavelength determined from a combination of a nucleic acid and a fluorescent dye is detected by a housing (24) that fixes the unit (22) in a predetermined position and blocks light from the outside to the object to be inspected. Enable It is an inspection apparatus according to claim.

  According to a second aspect of the present invention, the light source has a light emission wavelength range set according to the types of nucleic acid and fluorescent dye.

  A third aspect of the present invention is characterized in that the optical filter has a passing wavelength range set according to the types of nucleic acid and fluorescent dye.

In the present invention, a nucleic acid-containing ink layer is provided in a very small region, and the region is irradiated with excitation light having a plurality of different wavelengths with respect to a medium for authenticity determination in which the concentration of the nucleic acid contained therein is set as a detection limit. Thus, authenticity can be determined only by detecting the presence or absence of light having a specific wavelength determined from the combination of the nucleic acid and the fluorescent dye.
As described above, according to the present invention, there is an effect that it is possible to perform an extremely highly reliable test as compared with a conventional simple fluorescence detecting device while being a simple device.

It is a schematic sectional drawing which shows the layer structure of the transfer body employ | adopted as one means which provides the nucleic acid containing ink layer used as the test object by the test | inspection apparatus of this invention in a very small area | region. It is a schematic sectional drawing which shows the structure of the test | inspection apparatus of this invention. It is a circuit diagram which shows an example of the control means of the light source for excitation used for the test | inspection apparatus of this invention.

[1] First, an inspected object (authentication determination medium) to be inspected by the inspection apparatus of the present invention will be described. The object to be inspected is provided with a nucleic acid-containing ink layer in an extremely small area.
The layer structure of the transfer body employed as a means for providing the nucleic acid-containing ink layer in a very small area will be described as an example.

[1-1] Layer Configuration of Transfer Body As the transfer body according to the present invention, for example, as shown in FIG. 1, a transfer substrate 1, a hologram forming layer 2, a reflective thin film layer 3, an adhesive layer 4, a reflection And a transfer body having a basic structure composed of a nucleic acid-containing ink layer 5 partially provided between the conductive thin film layer 3 and the adhesive layer 4.

As another example, in order to smooth the unevenness on the reflective thin film layer composed of the portion provided with the nucleic acid-containing ink layer and the portion not provided, a smoothing layer for embedding the concave portion is laminated, Next, a transfer body in which an adhesive layer is laminated can be mentioned.
As another example, a release layer and a protective layer are provided between the transfer substrate 1 and the hologram forming layer 2, and the transfer substrate / release layer / protective layer / hologram forming layer / reflective thin film layer / nucleic acid-containing ink. A transfer body having a layer structure of a layer / adhesive layer can also be used (in the present invention, “/” used between layers means a boundary surface between layers). Here, it may be a layer structure which does not have any one of a exfoliation layer and a protection layer.

(Transfer substrate)
The transfer substrate used in the transfer body serves as a support for laminating a transfer layer to be transferred to the transfer target during production of the transfer body. The transfer body of the present invention is superimposed on the article to be transferred so that the adhesive layer faces the article surface, and the transfer layer is transferred from the transfer substrate as the support to the article surface, and then printed. Then, the transfer substrate 1 is peeled off and removed.

(Peeling layer)
As a layer constituting a part of the transfer layer to be transferred from the transfer base material to the article to be transferred by heating at the time of transfer printing of the transfer body, in some cases, a peeling layer is provided as a layer in direct contact with the base material. It may be formed. By having this release layer, the transfer layer can be reliably and easily transferred from the thermal transfer sheet to the transfer medium.

(Protective layer)
As a layer constituting a part of the transfer layer provided on the transfer substrate of the transfer body, a protective layer is optionally formed as a layer for directly contacting the hologram forming layer described below and protecting the layer. May be.

(Hologram forming layer)
As a layer constituting a part of the transfer layer provided on the transfer substrate of the transfer body, on the transfer substrate, or when a release layer is provided, and / or when a protective layer is provided. A hologram forming layer for forming a hologram may be provided on the protective layer.
In the transfer body, the hologram formed by the hologram forming layer may be any conventionally known hologram, or a combination thereof. For example, it may be a surface relief type hologram forming layer with irregularities formed on its surface, and a volume type characterized in that a hologram is formed by recording interference fringes in the forming material It may be a hologram forming layer. In particular, the surface relief hologram is preferable in that the foil breakage is good at the time of transfer printing by heat.

(Reflective thin film layer)
In the transfer body, a reflective thin film layer is partially or entirely provided on the transfer substrate, the release layer or the protective layer, in order to enhance the reflection and / or diffraction effect of the hologram. Form. As a formation method, a known method such as sublimation, vapor deposition, CVD (chemical vapor deposition), sputtering, reactive sputtering, ion plating, electroplating or the like can be used.
As the reflective thin film layer, any film can be used as long as it can exhibit the hologram effect. For example, a metallic glossy thin film layer such as a metal thin film by a vacuum thin film method or a transparent reflective thin film layer can be used. Either is acceptable.

The transparent reflective thin film layer has a nearly colorless and transparent hue, and its optical refractive index is different from that of the hologram forming layer, so that it is possible to visually recognize the glitter of a hologram or the like even though there is no metallic luster. A transparent hologram can be produced. For example, there are a thin film having a higher refractive index than the hologram forming layer and a thin film having a lower refractive index. Examples of the former include ZnS, TiO ₂ , Al ₂ O ₃ , Sb ₂ S ₃ , SiO, and SnO _2. ITO, etc., and examples of the latter include LiF, MgF ₂ , and AlF ₃ . Preferably, it is a metal oxide or nitride, specifically, Be, Mg, Ca, Cr, Mn, Cu, Ag, Al, Sn, In, Te, Fe, Co, Zn, Ge, Pb, Cd , Bi, Se, Ga, Rb, Sb, Pb, Ni, Sr, Ba, La, Ce, Au, and other oxides or nitrides, and the like can be exemplified by a mixture of two or more thereof. Also, a general light-reflective metal thin film such as aluminum can be used when it has a thickness of 200 mm or less. The transparent metal compound may be formed on the hologram forming layer so as to have a thickness of about 10 to 2000 nm, preferably 20 to 1000 nm, as with the metal thin film.

[1-2] Nucleic acid-containing ink layer In the transfer body, a nucleic acid-containing ink layer is partially provided on the reflective thin film layer. The nucleic acid-containing ink layer is a layer made of a nucleic acid-containing ink composition containing a nucleic acid and a fluorescent dye.

(Nucleic acid)
The nucleic acid is DNA (including nuclear DNA and extranuclear DNA), RNA, and derivatives thereof, which may be naturally derived or artificial, and the nucleotides constituting the nucleic acid are naturally It may exist or it may be manufactured artificially. Further, the nucleic acid may be in either a single stranded fragment or a double stranded fragment, and its length is an oligomer of less than about 100 bases / base pair, depending on the authentication application. Or a polymer composed of more nucleotides. For the purpose of individual identification, it is preferable to use a nucleic acid fragment having a length of preferably 10 bases / base pair or more, more preferably 100 bases / base pair or more. In addition, from the viewpoint of handling during production and detection, storage stability, ease of degradation, etc., the length of each fragment is 1000 bases / base pair or less, more suitably 500 bases / base pair or less. It is preferable to synthesize them as described above, or to cleave them with restriction enzymes.

In the medium for authenticity determination to be inspected by the inspection apparatus of the present invention, a DNA obtained by pouring DNA from a living body, for example, a human cell, and decomposing it with, for example, a restriction enzyme is used in addition to the nucleic acid-containing ink composition. You may mix with the component of. In addition, a specific sequence, preferably a specific polymorphic region, preferably a STR sequence can be amplified by PCR and used. Furthermore, DNA or RNA derived from non-human animals and plant cells can also be used. On the other hand, a nucleic acid synthesized by a conventionally known chemical synthesis method may be used.
The nucleic acid obtained as described above may consist of only a fragment having one type of sequence or a mixture of fragments having different sequences, as necessary.
The nucleic acid is preferably blended at a ratio of 1 × 10 ⁻² to 1 × 10 ⁵ μg, preferably 1 to 1 × 10 ⁴ μg, based on 100 g of the solid content in the nucleic acid-containing ink composition. It is essential that one or more nucleic acids are contained in the nucleic acid-containing ink layer. When the amount is less than 1 × 10 ⁻² μg, a specific site provided with the nucleic acid-containing ink layer is cut out from the transfer body, and the nucleic acid is removed. It can be difficult to collect and sequence. On the other hand, when the amount is more than 1 × 10 ⁵ μg, it is difficult to match the refractive index of the adhesive layer or the smoothing layer surrounding the nucleic acid-containing ink layer, and it is easy to visually recognize from the outside, which is not preferable. In addition, the probability of contamination in which nucleic acids are mixed outside the target composition during production is also increased.

(Fluorescent dye)
Moreover, in the nucleic acid containing ink composition which comprises the said nucleic acid containing ink layer, you may use the various fluorescent dyes generally used conventionally from the fluorescent dye.
Specifically, organic fluorescent dyes and inorganic fluorescent dyes can be used. Examples of organic fluorescent dyes include diaminostilbene disulfonic acid derivatives, imidazole derivatives, coumarin derivatives, triazoles, carbazoles, pyridine, naphthalic acid, imidazolone derivatives, fluorescein, etc. And dyes such as eosin, and compounds having a benzene ring such as anthracene. In addition, examples of the inorganic fluorescent dye include, for example, oxides such as Ca, Ba, Mg, and Sr, crystals such as sulfides, silicates, phosphates, and tungstates as main components, and Eu, Mn, and Pb. , Fe, Mn, Zn, Ag, Cu and other metal elements or rare earth elements can be used as a dopant.

  Further, when the nucleic acid is double-stranded, a fluorescent dye that enters into the double helix structure and forms a double-stranded nucleic acid-fluorescent dye complex can be preferably used. In the nucleic acid-containing ink composition of the present invention, when such a combination of a fluorescent dye and a double-stranded nucleic acid is used, the electron cloud of the fluorescent dye has a strong interaction with an electron cloud of a base pair having a double helix structure. Demonstrates that the double helix structure is physically strong, and the resistance to degradation and stability are increased. Such fluorescent dyes do not have a three-dimensional structure, have a two-dimensional planar structure, have a size of about 0.4 nm × 1.0 to 1.4 nm, and have three benzene rings connected to each other. (Anthracene skeleton, phenanthrene skeleton) or those having one skeleton linked to two benzene rings as a substituent. Specifically, anthracene skeleton, phenanthrene skeleton, dibenzofuran skeleton, anthraquinone skeleton, indigo, thioindigo skeleton, diphenylmethane skeleton, triphenylmethane skeleton, xanthene skeleton, anthocyanin skeleton, fluorescein skeleton, rhodamine skeleton, thiazine skeleton, benzidine skeleton, ditrizine Examples include skeletons, dianisidine skeletons, diaminoacridine skeletons and the like in which auxiliary color groups are substituted. These fluorescent dyes have a carbocyclic or heterocyclic skeleton in one plane, and an electron cloud composed of π electron linkages on the plane above and below the plane. Are at a distance of about 0.2 nm and express interaction, and both ends of the fluorescent dye interact with oxygen in the phosphate / ribosome structure constituting the pillar of the DNA helical structure. One benzene ring (phenyl group) is added to the phenanthrene skeleton, and two amino groups as an auxiliary color group, ethidium bromide, acridine orange, cyber green I and II, and a rare earth complex as a substituent to the naphthalene diimide skeleton The fluorescent dye is a typical fluorescent dye capable of forming the complex, and can be preferably used because of its physical stability.

In the medium for authenticity determination to be inspected according to the present invention, the nucleic acid-containing ink composition forms a complex by entering double-stranded DNA and a double helix structure from the viewpoint of physical stability. It is preferable to contain a combination with a fluorescent dye that can be used. Such a DNA-containing ink composition exhibits excellent structural stability over an extremely long period of time under the condition that the transfer material of the present invention is coated with the resin composition of the adhesive layer or the smoothing layer, and is excellent. And storage stability such as light resistance and weather resistance.
Furthermore, the fluorescent dye that forms a complex by entering into the double helix structure is affected by the non-covalent interaction, and the energy gap between the excited state and the ground state emitting fluorescence is increased. It is narrowed and a long wavelength shift of the fluorescence wavelength appears. For example, acridine orange normally emits fluorescence at 650 nm with respect to excitation light of 460 nm. However, when entering a double helical structure to form a complex, acridine orange emits fluorescence at 526 nm with respect to excitation light of 500 nm. . By strictly managing this wavelength shift, for example, a specific fluorescence wavelength is emitted by irradiating a specific excitation wavelength to a specific part without performing a destructive inspection by cutting out a transfer layer and collecting a nucleic acid. Whether or not non-destructive inspection is possible.

  In the authenticity determination medium to be inspected according to the present invention, the fluorescent dye is 0.001 to 1000 μg, preferably 0.01 to 100 μg, based on 100 g of the solid content in the nucleic acid-containing ink composition. It is preferable to mix. If it is less than 0.001 μg, the reliability in determining authenticity becomes insufficient, and if it is more than 1000 μg, the proportion of the fluorescent dye not incorporated in the nucleic acid increases and its stability decreases. At the same time, the presence of the fluorescent dye itself tends to be exposed, which is not preferable.

(Nucleic acid-containing ink composition)
The above nucleic acid and fluorescent dye can be inked by a conventionally known method. For example, a nucleic acid and a fluorescent dye can be appropriately mixed with a solvent and other components such as a vehicle and various additives to form an ink composition.

(Layer formation)
In the transfer body, the nucleic acid-containing ink composition obtained as described above can be appropriately printed and formed at an arbitrary thickness on an arbitrary portion on the reflective thin film layer. For printing, for example, a gravure coating method, an offset printing method, a letterpress printing method, a screen printing method, a curtain coating method, an ink jet method, or the like can be used.
The transfer body requires significantly higher reliability than the conventional anti-counterfeit technology, and the effect is further enhanced by bringing the concentration of the nucleic acid contained in the transfer body close to the detection limit. Therefore, it is important to prevent even slight contamination of nucleic acids in order to eliminate false detection as much as possible and perform precise detection. Therefore, the screen printing method, which can batch-process printing substrates, printing plates, inks, etc., prevents nucleic acid contamination and accurately spots the target position on the reflective thin film layer. Since printing can be performed, it can be particularly suitably used in the present invention. In particular, when the adhesion of the nucleic acid-containing ink composition to be used due to airborne droplets is confirmed by fluorescence detection or the like, a stainless screen printing method with high detection accuracy can be suitably used. Further, when screen printing is performed, it is easy to isolate the periphery of the printing area of the screen plate by making the ink supply unit completely sealed. In particular, it is desirable that the printing area of the nucleic acid-containing ink layer is a small area, and it is possible to print at one place with a size of about 10 μm ² in the screen printing method.

The nucleic acid-containing ink layer can be formed with an appropriate thickness depending on the size and concentration of the nucleic acid contained, but from the viewpoint of detection suitability and concealability, 0.1 to 5 μm, particularly 0.1. It is preferable to form with a thickness of ˜2 μm. Similarly, it is preferable to form in an area of 10 to 5 × 10 ⁸ μm ² , more preferably 100 to 5 × 10 ⁶ μm ² . At this time, the size, concentration, formation area, and formation of the nucleic acid are such that 10 ¹² to 10 ²⁰ , preferably 10 ¹² to 10 ¹⁵ nucleic acid fragments are present per 1 cm ³ occupied area of the nucleic acid-containing ink layer. It is preferable to adjust the thickness.

(Adhesive layer)
In the transfer body, an adhesive layer is provided after the nucleic acid-containing ink layer is partially provided on the reflective thin film layer.
The adhesive layer is not particularly limited as long as it is a heat-sensitive or pressure-sensitive adhesive that does not have adhesiveness at normal temperature or normal pressure, and exhibits an adhesive force only when heated or pressurized.

The resin as described above may be melted by heat to form a liquid, which may be applied and cooled by a roll coating method, a comma coating method, a hot malt applicator method, or the like.
Although the thickness of an adhesive bond layer can be set suitably, Preferably it is 1-5 micrometers, Most preferably, it is 2-4 micrometers. If it is 1 μm or less, sufficient adhesiveness may not be obtained, and if it is 5 μm or more, foil breakage may be poor during heating or pressurization.
You may add various additives, such as a white pigment, an extender pigment, and a fluorescent whitening agent, to the adhesive composition which comprises the said adhesive bond layer as needed.
In addition to the heat-sensitive or pressure-sensitive adhesive, it is possible to use various arbitrary pressure-sensitive adhesives. In that case, however, the release film that is peeled off when applied to the article surface is applied to the layer surface. Used by sticking.

(Smoothing layer (not shown))
In the transfer body, after partially providing the nucleic acid-containing ink layer on the reflective thin film layer, if necessary, the reflective thin film layer comprising a portion provided with the nucleic acid-containing ink layer and a portion not provided In order to smooth the upper unevenness, a smoothing layer for embedding the concave portion may be laminated, and then the adhesive layer may be laminated.

(Physical stability)
In the field of anti-counterfeiting as in the present invention, a transfer body is required to have a stable authenticity guarantee capability that is usually 1 year or longer, and further 5 years or longer. By having the structure of the present invention, even a nucleic acid that is relatively easily degradable can exhibit weather resistance, light resistance, and physical stability that can withstand the above requirements. In addition, each layer constituting the transfer layer, that is, at least one of a peeling layer, a protective layer, a hologram forming layer, a reflective thin film layer, a nucleic acid-containing ink layer, a smoothing layer, and an adhesive layer is provided with an ultraviolet absorber. By containing, deterioration over time due to ultraviolet rays or the like can be suppressed, the physical stability of the contained nucleic acid can be further increased, and sequence information can be preserved for a long period of time.

[2] Detection principle and detection device [2-1] Detection principle In the above, the nucleic acid-containing ink layer is preferably provided in a small area and is difficult to be visually recognized from the outside. Inconvenience may also occur for the detector. Therefore, in order to eliminate such inconvenience, at least one layer constituting the transfer layer may be provided with one or more detection marks for detecting the position of the portion where the nucleic acid-containing ink layer is provided. Good. As a result, a legitimate detector performs alignment of the article to which the transfer layer from the transfer body is applied with reference to the detection mark, and whether fluorescence is detected in the portion where the nucleic acid-containing ink layer is provided. It is checked whether the fluorescence wavelength is authentic, and whether the nucleic acid contained in the portion has valid sequence information.

Such a detection mark may be a visible print, a cutout, a part of a hologram image, or the like, or cannot be visually recognized under a normal environment like a laser reproduction hologram. Also good.
Furthermore, in order to counter the leakage of information and prevent unauthorized acquisition of nucleic acids, an ink composition that contains only fluorescent dyes and does not contain nucleic acids is provided in one or more parts of the transfer body to complicate detection. Also good.

[2-2] Detection Device The inspection device of the present invention will be described in detail with reference to FIGS.
FIG. 2 is a schematic cross-sectional view of the inspection apparatus 20 of the present invention. In the figure, 2 1 a light source, 23 is an optical filter, 22 detector, 24 housing, 25 denotes a mounting base.
A light source 21, a detection unit 22, and an optical filter 23 are attached to the casing 24 so as to have a predetermined positional relationship. In the example of FIG. 2, the optical filter 23 is attached to the light receiving side tip of the detection unit 22. The predetermined portion of the mounting table 25 to be inspected to the transfer layer 11 is transferred to the transfer member 12 is setting. The inspection apparatus 20 becomes a dark box as a whole by covering the casing 24 at a predetermined position of the mounting table so as to cover the object to be inspected set on the mounting table 25.
The form of the inspection apparatus 20 is not limited to the form shown in FIG. 2 as described above. For example, a form in which a bottom plate having an opening is provided in the housing 24 and the mounting table 25 is fitted into the opening is also possible. Good.

As the light source 21, for example, an LED (Light Emitting Diode) can be used. Since LEDs have various emission wavelengths and emission luminances, an LED having a predetermined wavelength is selected and used in the present invention.
Hereinafter, a case where two different wavelength (λ1, λ2) LEDs are used will be described. However, the number of LEDs is not limited to two. Furthermore, the light source 21 used in the present invention is not limited to an LED, and may be any light source that can emit light by switching two or more emission wavelengths.

  FIG. 3 is a circuit diagram showing an example of control means for performing LED switching control. FIG. 3A shows an example in which LEDs are connected in series to increase luminance. FIG. 3B shows an example in which LEDs are connected in parallel in order to increase light brightness. In both FIGS. 3A and 3B, the emission wavelength of the LED 1 is λ1, and the emission wavelength of the LED 2 is λ2 (λ1 ≠ λ2). The wavelength λ of the excitation light P1 emitted from the light source 21 can be changed by switching the switches (SW1, SW2) connected to the LEDs 1 and 2. In the figure, E is a driving power supply, and R1 and R2 are current adjusting resistors.

As the optical filter 23, a band-pass type filter that allows light in a predetermined wavelength range to pass therethrough is used.
Although not shown, a plurality of optical filters 23 having different pass wavelength ranges are prepared, and the pass wavelength range can be selected by switching these or overlapping the filters.
Hereinafter, although the case where the optical filter of two different wavelength ranges is used is demonstrated, it is not limited to two, What is necessary is just two or more.
As the detection unit 22, for example, a photo sensor typified by a photodiode, a phototransistor, a photoelectric tube, a photomultiplier tube, or a camera equipped with an image sensor can be used.

Excitation light P1 emitted from the LED selected by the control means in FIG. 3 is irradiated onto the nucleic acid-containing ink layer 5 formed at a predetermined location of the object to be inspected. If the predetermined nucleic acid and fluorescence are present in the ink, excitation is performed. Light P2 having a specific wavelength corresponding to the type of nucleic acid and fluorescent dye is excited by the action of light P1.
The optical filter 23 passes most of the light P2 having the specific wavelength and cuts most of light having a wavelength other than the light P2. The light P2 that has passed through the optical filter 23 is detected by the detector 22.
As described above, according to the present invention, the combination of the type of the nucleic acid and the fluorescent dye contained in the nucleic acid-containing ink layer 5 from the wavelength of the excitation light P1 and the light P2 having a specific wavelength passing through the optical filter 23 is predetermined. Can be determined.

[Example]
For the evaluation of the inspection apparatus of the present invention, a sample A using a nucleic acid-containing ink composed of a nucleic acid and a fluorescent dye and a sample B using a nucleic acid-free ink in which the nucleic acid is removed from the nucleic acid-containing ink and the other components are the same are prepared. did.
The details of the prototype sample itself will be described later.

In accordance with the characteristics of Samples A and B, light sources (LED1, LED2) and optical filters (FL1, FL2) having the following characteristics were prepared.
LED1 ... The emission wavelength range is 498-510nm (blue-green light-emitting diode)
LED2 ... The emission wavelength range is 390-412nm (ultraviolet light emitting diode)
FL1 ··· Center value of the passing wavelength range 525nm
FL2 ··· Center value of passing wavelength range 615nm

Result of detection test by the inspection apparatus of the present invention (1) Test result of sample A (nucleic acid + fluorescent dye) Light was detected as a result of irradiating light from LED1 and detecting light through FL1.
As a result of irradiating the light of LED1 and detecting the light through FL2, no light was detected.
As a result of irradiating the light of LED2 and detecting the light through FL1, no light was detected.
As a result of irradiating the light of LED2 and detecting the light through FL2, no light was detected.
(2) Test result of sample B (only fluorescent dye) Light was detected as a result of irradiating light from LED1 and detecting light through FL1.
As a result of irradiating the light of LED1 and detecting the light through FL2, no light was detected.
As a result of irradiating the light of LED2 and detecting the light through FL1, no light was detected.
Light was detected as a result of irradiating the light of LED2 and detecting the light through FL2.

From the above results, based on the combination of the wavelength of the light source (LED) and the pass wavelength range of the optical filter (FL), the sample is made of ink composed of both nucleic acid and fluorescent dye, or only fluorescent dye It is possible to determine whether the ink is composed of
That is, the inspection apparatus of the present invention makes it possible to make a primary determination as to whether or not the nucleic acid-containing ink is contained in a predetermined portion of the object to be inspected.

Hereafter, the structure of the sample used for the said test is explained in full detail.
(1) Configuration of Sample A A thermosetting resin composition (Iupimer LZ, manufactured by The Inktec Co., Ltd.) made of a melamine resin was applied to the surface of a PET film having a thickness of 12 μm as a transfer substrate, A surface relief hologram is formed by bringing a surface relief hologram reproduction mold surface having an image position detection pattern (a diffraction grating of 1000 μm × 500 μm size) into contact with the coated surface, and subjecting it to a heat curing treatment. A hologram forming layer having a thickness of 3 μm was obtained.
Subsequently, ZnS was vacuum-deposited on the surface on which the relief hologram was formed, thereby forming a reflective transparent thin film layer having a thickness of 50 nm.

  On the other hand, salmon egg white-derived DNA (manufactured by Nichiro Co., Ltd., DNA solution extracted from salmon egg white, fragmented to 300 bp or less) in sterile water 74 μl (μl: microliter) 1.3 mg (4000 nmol base) solution And a solution of 20 nmol (1% / base pair) of fluorescent dye (acridine orange) in 3 μl of sterilized water and 6000 nmol of alkyl lipid (C18 lipid) in 24 μl of sterilized water (1.5 times per base). A solution was prepared, these were mixed, and the precipitate was collected to remove excess alkyl lipid, washed, freeze-dried, and then 28 mg of a surfactant-treated DNA / fluorescent dye complex was obtained. 1 mg of the obtained complex was dissolved in 50 μl of ethanol and mixed with 10 g of cellulose resin / acrylic resin (2: 1) and 1 g of ethanol / ethyl acetate (2: 1) to obtain a nucleic acid-containing ink composition.

The obtained nucleic acid-containing ink composition is silk-screen printed at a predetermined position on the reflective thin film layer while detecting an image position detection pattern, dried at 50 ° C. for 10 minutes, and then contains a nucleic acid having a size of 200 μm × 200 μm. An ink layer (thickness 2 μm, refractive index n = 1.55) was formed.
An adhesive composition having the following composition was applied thereon and dried at 70 ° C. for 10 minutes to form an adhesive layer (thickness 3 μm, refractive index n = 1.56). Obtained.
<Adhesive composition>
・ Cellulose resin / acrylic resin (2: 1) (manufactured by Seiko Advance Co., Ltd., STR T475) 30 parts by mass ・ External pigment (calcium carbonate) 5 parts by mass ・ Methyl isobutyl ketone 25 parts by mass ・ Ethyl acetate 40 parts by mass

When the transfer body 10 of the present invention obtained above was superposed so that the adhesive layer 4 and the surface of the transfer target 12 face each other and transferred by a thermal printer, a good hologram reproduction image was confirmed by visual observation. It was done.
In addition, the said part was cut out, DNA was melt | dissolved and extracted, the PCR amplification process was carried out, the base sequence of the contained DNA fragment was read with the DNA sequencer, and it was confirmed that it was the target salmon egg white origin DNA.

(2) Configuration of Sample B In the same manner as Sample A, a transfer body was obtained. However, instead of the step of partially providing the nucleic acid-containing ink layer in Sample A, a nucleic acid-free ink composition having the following composition is printed at another position on the reflective thin film layer, and has a size of 200 μm × 200 μm. A nucleic acid-free ink layer (thickness 2 μm, refractive index n = 1.55) was formed.
<Nucleic acid-free ink composition>
・ Fluorescent dye (acridine orange) 20 nmol / 3 μl sterilized water ・ Cellulose resin / acrylic resin (2: 1) (manufactured by Seiko Advance, S
TR T475) 10g
・ Ethanol / ethyl acetate (2: 1) 1g
In the same manner as in sample A, transfer printing was performed on the transfer medium, and the hologram reproduction image, fluorescence, and DNA sequence were confirmed well.

1. 1. Transfer substrate 2. Hologram forming layer Reflective thin film layer 4. 4. Adhesive layer 9. Nucleic acid-containing ink layer Transfer body 11. Transfer layer 12. Transfer object 20. Inspection device 21. Light source 22. Detection unit 23. Optical filter 24. Enclosure

Claims (3)

An inspection apparatus for an object to be inspected that includes at least a part of a nucleic acid-containing ink layer containing a nucleic acid and a fluorescent dye,
A mounting table for setting the object to be inspected at a predetermined position;
A light source that irradiates the test object with a plurality of different wavelengths of excitation light;
An optical filter that passes at least a part of the light emitted by being excited by the excitation light in a predetermined wavelength region irradiated on the object to be inspected;
A detector for detecting light that has passed through the optical filter;
A housing that fixes the light source, the optical filter, and the detection unit at predetermined positions, and blocks light from the outside of the object to be inspected;
An inspection apparatus characterized in that it can detect light having a specific wavelength determined from a combination of a nucleic acid and a fluorescent dye.
The inspection apparatus according to claim 1, wherein the light source has a light emission wavelength range set in accordance with types of nucleic acid and fluorescent dye. The inspection apparatus according to claim 1, wherein the optical filter has a passing wavelength range set in accordance with types of nucleic acid and fluorescent dye.

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