JP5851401B2 - 安定な補酵素による酵素の安定化 - Google Patents

安定な補酵素による酵素の安定化 Download PDF

Info

Publication number
JP5851401B2
JP5851401B2 JP2012525165A JP2012525165A JP5851401B2 JP 5851401 B2 JP5851401 B2 JP 5851401B2 JP 2012525165 A JP2012525165 A JP 2012525165A JP 2012525165 A JP2012525165 A JP 2012525165A JP 5851401 B2 JP5851401 B2 JP 5851401B2
Authority
JP
Japan
Prior art keywords
enzyme
stabilized
coenzyme
dehydrogenase
stored
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012525165A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013502212A (ja
JP2013502212A5 (https=
Inventor
レーデル、ヴォルフガング
ホルン、カリーナ
シュタインケ、ネリ
ブッチ、ナディーネ
マイアー、トーマス
シュマック、ライナー
ナーゲル、ロルフ
ハインドル、ディーター
Original Assignee
エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト
エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト, エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト filed Critical エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト
Publication of JP2013502212A publication Critical patent/JP2013502212A/ja
Publication of JP2013502212A5 publication Critical patent/JP2013502212A5/ja
Application granted granted Critical
Publication of JP5851401B2 publication Critical patent/JP5851401B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/05—Oxidoreductases acting on NADH or NADPH (1.6) with a quinone or similar compound as acceptor (1.6.5)
    • C12Y106/05002—NAD(P)H dehydrogenase (quinone) (1.6.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2012525165A 2009-08-20 2010-08-18 安定な補酵素による酵素の安定化 Active JP5851401B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09168327A EP2292751A1 (de) 2009-08-20 2009-08-20 Stabilisierung von Enzymen mit stabilen Coenzymen
EP09168327.6 2009-08-20
PCT/EP2010/062045 WO2011020856A1 (de) 2009-08-20 2010-08-18 Stabilisierung von enzymen mit stabilen coenzymen

Publications (3)

Publication Number Publication Date
JP2013502212A JP2013502212A (ja) 2013-01-24
JP2013502212A5 JP2013502212A5 (https=) 2013-08-15
JP5851401B2 true JP5851401B2 (ja) 2016-02-03

Family

ID=41211921

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012525165A Active JP5851401B2 (ja) 2009-08-20 2010-08-18 安定な補酵素による酵素の安定化

Country Status (8)

Country Link
US (2) US9540702B2 (https=)
EP (2) EP2292751A1 (https=)
JP (1) JP5851401B2 (https=)
KR (2) KR101554457B1 (https=)
CN (1) CN102471767B (https=)
CA (1) CA2770249C (https=)
MX (1) MX2012001946A (https=)
WO (1) WO2011020856A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102471793B (zh) * 2009-07-27 2014-04-30 霍夫曼-拉罗奇有限公司 Carba-nad的酶促合成
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
US8787944B2 (en) 2011-08-18 2014-07-22 Rivada Research, Llc Method and system for providing enhanced location based information for wireless handsets
EP2636750A1 (en) 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
US20150192575A1 (en) * 2012-08-09 2015-07-09 Stichting Dienst Landbouwkundig Onderzoek Membrane assembly and a lateral flow immunoassay device comprising such membrane assembly
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
EP2770064A1 (de) * 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
EP3097406A1 (en) 2014-01-24 2016-11-30 Roche Diabetes Care GmbH Method of manufacturing uni- and no-code test stripes
KR102372113B1 (ko) 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들
EP3339431A1 (en) * 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
EP3963058A4 (en) 2019-05-01 2024-01-03 Codexis, Inc. MODIFIED GLUCOSE DEHYDROGENASES AND PROCESSES FOR THE REDUCTIVE AMINATION OF KETONE AND AMINO COMPOUNDS
WO2020242967A1 (en) * 2019-05-28 2020-12-03 W. L. Gore & Associates, Inc. Stabilized medical device and method for making it
JP7473632B2 (ja) * 2019-08-30 2024-04-23 アボット ダイアベティス ケア インコーポレイテッド アルコールの検出のための分析物センサ

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2061984C3 (de) 1970-12-16 1974-04-11 Boehringer Mannheim Gmbh, 6800 Mannheim Reagens zur Bestimmung der Lactat-Dehydrogenase im Farbtest
US5032506A (en) 1986-12-16 1991-07-16 Enzymatics, Inc. Color control system
US5609718A (en) 1995-09-29 1997-03-11 Micron Technology, Inc. Method and apparatus for measuring a change in the thickness of polishing pads used in chemical-mechanical planarization of semiconductor wafers
DE19543493A1 (de) * 1995-11-22 1997-05-28 Boehringer Mannheim Gmbh Stabilisierte Coenzym-Lösungen und deren Verwendung zur Bestimmung von Dehydrogenasen bzw. deren Substrate im alkalischen Milieu
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
US6710770B2 (en) * 2000-02-11 2004-03-23 Canesta, Inc. Quasi-three-dimensional method and apparatus to detect and localize interaction of user-object and virtual transfer device
AU2001262702A1 (en) 2000-06-07 2001-12-17 Asahi Kasei Kabushiki Kaisha Coenzyme derivatives and enzymes appropriate therefor
CN100439513C (zh) 2002-05-16 2008-12-03 霍夫曼-拉罗奇有限公司 含有非再生酶-辅酶复合体的方法和试剂体系
CA2535147A1 (en) 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
DE102005035461A1 (de) * 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stabile NAD/NADH-Derivate
DE102006035020B4 (de) * 2005-07-28 2018-08-23 Roche Diabetes Care Gmbh Stabilisierung von NAD/NADH
US7553615B2 (en) 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
US20070196899A1 (en) 2005-11-29 2007-08-23 Sony Corporation Mutant protein having diaphorase activity
GB0625671D0 (en) 2006-12-21 2007-01-31 Oxford Biosensors Ltd Protein formulation
EP1964927A1 (de) * 2007-02-27 2008-09-03 F. Hoffmann-La Roche AG Chinone als Mediatoren für photometrische Teste
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
EP2287605A1 (de) * 2009-08-20 2011-02-23 Roche Diagnostics GmbH Vereinfachte Magazinierung integrierter Systeme
EP2333544A1 (de) * 2009-12-11 2011-06-15 F. Hoffmann-La Roche AG Sterilisierbare Chemie für Testelemente
MX2012006706A (es) * 2009-12-16 2012-07-03 Hoffmann La Roche Deteccion de la descomposicion de las enzimas en un elemento de prueba mediante la liberacion controlada de analito protegido.

Also Published As

Publication number Publication date
CN102471767A (zh) 2012-05-23
CA2770249C (en) 2015-04-28
JP2013502212A (ja) 2013-01-24
MX2012001946A (es) 2012-04-02
EP2467478A1 (de) 2012-06-27
EP2292751A1 (de) 2011-03-09
KR20120064076A (ko) 2012-06-18
US20170073661A1 (en) 2017-03-16
WO2011020856A1 (de) 2011-02-24
US20120276565A1 (en) 2012-11-01
CN102471767B (zh) 2018-02-16
KR101554457B1 (ko) 2015-09-30
US9540702B2 (en) 2017-01-10
EP2467478B1 (de) 2015-09-16
CA2770249A1 (en) 2011-02-24
KR20150070443A (ko) 2015-06-24

Similar Documents

Publication Publication Date Title
JP5851401B2 (ja) 安定な補酵素による酵素の安定化
US11220674B2 (en) Stabilization of dehydrogenases with stable coenzymes
US20160333391A1 (en) Methods of producing sterilized diagnostic test elements
JP5777633B2 (ja) 保護された分析物の制御された放出によるテストエレメントにおける酵素の分解の検出
AU2012203453B2 (en) Stabilization of dehydrogenases using stable coenzymes
HK1152728B (en) Stabilization of dehydrogenases using stable coenzymes
HK1152728A (en) Stabilization of dehydrogenases using stable coenzymes
HK1170770B (en) Stabilization of enzymes using stable coenzymes
HK1170770A (en) Stabilization of enzymes using stable coenzymes
NZ587397A (en) Stabilization of dehydrogenases using stable chemically modified coenzymes
HK1174390A (en) Sterilizing chemistry for test elements

Legal Events

Date Code Title Description
A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20130627

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130627

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20141021

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150121

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20150121

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150616

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151015

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20151026

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20151110

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20151202

R150 Certificate of patent or registration of utility model

Ref document number: 5851401

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250