CN102471767B - 利用稳定辅酶的酶的稳定化 - Google Patents

利用稳定辅酶的酶的稳定化 Download PDF

Info

Publication number
CN102471767B
CN102471767B CN201080036800.9A CN201080036800A CN102471767B CN 102471767 B CN102471767 B CN 102471767B CN 201080036800 A CN201080036800 A CN 201080036800A CN 102471767 B CN102471767 B CN 102471767B
Authority
CN
China
Prior art keywords
enzyme
nad
coenzyme
stabilized
glucose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080036800.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN102471767A (zh
Inventor
W.勒德尔
C.霍恩
N.施泰因克
N.布西
T.迈尔
R.施姆克
R.纳格尔
D.海因德尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN102471767A publication Critical patent/CN102471767A/zh
Application granted granted Critical
Publication of CN102471767B publication Critical patent/CN102471767B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/05—Oxidoreductases acting on NADH or NADPH (1.6) with a quinone or similar compound as acceptor (1.6.5)
    • C12Y106/05002—NAD(P)H dehydrogenase (quinone) (1.6.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201080036800.9A 2009-08-20 2010-08-18 利用稳定辅酶的酶的稳定化 Active CN102471767B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09168327A EP2292751A1 (de) 2009-08-20 2009-08-20 Stabilisierung von Enzymen mit stabilen Coenzymen
EP09168327.6 2009-08-20
PCT/EP2010/062045 WO2011020856A1 (de) 2009-08-20 2010-08-18 Stabilisierung von enzymen mit stabilen coenzymen

Publications (2)

Publication Number Publication Date
CN102471767A CN102471767A (zh) 2012-05-23
CN102471767B true CN102471767B (zh) 2018-02-16

Family

ID=41211921

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080036800.9A Active CN102471767B (zh) 2009-08-20 2010-08-18 利用稳定辅酶的酶的稳定化

Country Status (8)

Country Link
US (2) US9540702B2 (https=)
EP (2) EP2292751A1 (https=)
JP (1) JP5851401B2 (https=)
KR (2) KR101554457B1 (https=)
CN (1) CN102471767B (https=)
CA (1) CA2770249C (https=)
MX (1) MX2012001946A (https=)
WO (1) WO2011020856A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102471793B (zh) * 2009-07-27 2014-04-30 霍夫曼-拉罗奇有限公司 Carba-nad的酶促合成
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
US8787944B2 (en) 2011-08-18 2014-07-22 Rivada Research, Llc Method and system for providing enhanced location based information for wireless handsets
EP2636750A1 (en) 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
US20150192575A1 (en) * 2012-08-09 2015-07-09 Stichting Dienst Landbouwkundig Onderzoek Membrane assembly and a lateral flow immunoassay device comprising such membrane assembly
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
EP2770064A1 (de) * 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
EP3097406A1 (en) 2014-01-24 2016-11-30 Roche Diabetes Care GmbH Method of manufacturing uni- and no-code test stripes
KR102372113B1 (ko) 2016-10-05 2022-03-07 에프. 호프만-라 로슈 아게 다중 분석물 진단 테스트 엘리먼트들을 위한 검출 시약들 및 전극 배열들, 그리고 그것을 사용하는 방법들
EP3339431A1 (en) * 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
EP3963058A4 (en) 2019-05-01 2024-01-03 Codexis, Inc. MODIFIED GLUCOSE DEHYDROGENASES AND PROCESSES FOR THE REDUCTIVE AMINATION OF KETONE AND AMINO COMPOUNDS
WO2020242967A1 (en) * 2019-05-28 2020-12-03 W. L. Gore & Associates, Inc. Stabilized medical device and method for making it
JP7473632B2 (ja) * 2019-08-30 2024-04-23 アボット ダイアベティス ケア インコーポレイテッド アルコールの検出のための分析物センサ

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101233144A (zh) * 2005-07-28 2008-07-30 霍夫曼-拉罗奇有限公司 稳定的nad/nadh衍生物
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2061984C3 (de) 1970-12-16 1974-04-11 Boehringer Mannheim Gmbh, 6800 Mannheim Reagens zur Bestimmung der Lactat-Dehydrogenase im Farbtest
US5032506A (en) 1986-12-16 1991-07-16 Enzymatics, Inc. Color control system
US5609718A (en) 1995-09-29 1997-03-11 Micron Technology, Inc. Method and apparatus for measuring a change in the thickness of polishing pads used in chemical-mechanical planarization of semiconductor wafers
DE19543493A1 (de) * 1995-11-22 1997-05-28 Boehringer Mannheim Gmbh Stabilisierte Coenzym-Lösungen und deren Verwendung zur Bestimmung von Dehydrogenasen bzw. deren Substrate im alkalischen Milieu
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
US6710770B2 (en) * 2000-02-11 2004-03-23 Canesta, Inc. Quasi-three-dimensional method and apparatus to detect and localize interaction of user-object and virtual transfer device
AU2001262702A1 (en) 2000-06-07 2001-12-17 Asahi Kasei Kabushiki Kaisha Coenzyme derivatives and enzymes appropriate therefor
CN100439513C (zh) 2002-05-16 2008-12-03 霍夫曼-拉罗奇有限公司 含有非再生酶-辅酶复合体的方法和试剂体系
CA2535147A1 (en) 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
DE102006035020B4 (de) * 2005-07-28 2018-08-23 Roche Diabetes Care Gmbh Stabilisierung von NAD/NADH
US7553615B2 (en) 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
US20070196899A1 (en) 2005-11-29 2007-08-23 Sony Corporation Mutant protein having diaphorase activity
GB0625671D0 (en) 2006-12-21 2007-01-31 Oxford Biosensors Ltd Protein formulation
EP1964927A1 (de) * 2007-02-27 2008-09-03 F. Hoffmann-La Roche AG Chinone als Mediatoren für photometrische Teste
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
EP2287605A1 (de) * 2009-08-20 2011-02-23 Roche Diagnostics GmbH Vereinfachte Magazinierung integrierter Systeme
EP2333544A1 (de) * 2009-12-11 2011-06-15 F. Hoffmann-La Roche AG Sterilisierbare Chemie für Testelemente
MX2012006706A (es) * 2009-12-16 2012-07-03 Hoffmann La Roche Deteccion de la descomposicion de las enzimas en un elemento de prueba mediante la liberacion controlada de analito protegido.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101233144A (zh) * 2005-07-28 2008-07-30 霍夫曼-拉罗奇有限公司 稳定的nad/nadh衍生物
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
CN101945999A (zh) * 2008-02-19 2011-01-12 霍夫曼-拉罗奇有限公司 使用稳定辅酶的脱氢酶稳定作用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
An investigation of the nicotinamide-adenine dinucleotide-induced tightening of the structure of glyceraldehyde 3-phosphate dehydrogenase;Osborne H H et al;《Biochem .J.》;19761231;第157卷(第1期);255-259 *
Separation and Properties of Two Novel NADP+-dependent Alcohol Dehydrogenases from Euglena gracilis Z;Iqbal Munir et al;《Pakistan Journal of Biological Sciences》;20061231;第9卷(第15期);2743-2747 *

Also Published As

Publication number Publication date
CN102471767A (zh) 2012-05-23
CA2770249C (en) 2015-04-28
JP2013502212A (ja) 2013-01-24
MX2012001946A (es) 2012-04-02
EP2467478A1 (de) 2012-06-27
EP2292751A1 (de) 2011-03-09
KR20120064076A (ko) 2012-06-18
US20170073661A1 (en) 2017-03-16
WO2011020856A1 (de) 2011-02-24
US20120276565A1 (en) 2012-11-01
KR101554457B1 (ko) 2015-09-30
JP5851401B2 (ja) 2016-02-03
US9540702B2 (en) 2017-01-10
EP2467478B1 (de) 2015-09-16
CA2770249A1 (en) 2011-02-24
KR20150070443A (ko) 2015-06-24

Similar Documents

Publication Publication Date Title
CN102471767B (zh) 利用稳定辅酶的酶的稳定化
US11220674B2 (en) Stabilization of dehydrogenases with stable coenzymes
KR101604624B1 (ko) 건식 화학 층에서 낮은 활성을 갖는 효소의 고속 반응 속도
HK1170770B (en) Stabilization of enzymes using stable coenzymes
HK1170770A (en) Stabilization of enzymes using stable coenzymes
AU2012203453B2 (en) Stabilization of dehydrogenases using stable coenzymes
JP3775561B2 (ja) 安定なｐｑｑ依存性グルコースデヒドロゲナーゼ組成物
HK1152728B (en) Stabilization of dehydrogenases using stable coenzymes
HK1152728A (en) Stabilization of dehydrogenases using stable coenzymes
NZ587397A (en) Stabilization of dehydrogenases using stable chemically modified coenzymes
HK1166353A (en) Fast reaction kinetics of enzymes having low activity in dry chemistry layers

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1170770

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant