MX2012001946A - Estabilizacion de enzimas utilizando coenzimas estables. - Google Patents

Estabilizacion de enzimas utilizando coenzimas estables.

Info

Publication number
MX2012001946A
MX2012001946A MX2012001946A MX2012001946A MX2012001946A MX 2012001946 A MX2012001946 A MX 2012001946A MX 2012001946 A MX2012001946 A MX 2012001946A MX 2012001946 A MX2012001946 A MX 2012001946A MX 2012001946 A MX2012001946 A MX 2012001946A
Authority
MX
Mexico
Prior art keywords
enzymes
stabilization
enzyme
stable coenzymes
stabilized
Prior art date
Application number
MX2012001946A
Other languages
English (en)
Inventor
Wolfgang Roedel
Thomas Meier
Rainer Schmuck
Dieter Heindl
Carina Horn
Nelli Steinke
Nadine Bucci
Rolf Nagel
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2012001946A publication Critical patent/MX2012001946A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/05—Oxidoreductases acting on NADH or NADPH (1.6) with a quinone or similar compound as acceptor (1.6.5)
    • C12Y106/05002—NAD(P)H dehydrogenase (quinone) (1.6.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se relaciona con un método para estabilizar una enzima mediante almacenamiento de la enzima en presencia de una coenzima estabilizada. Además, la presente invención se relaciona con una enzima estabilizada con una coenzima estabilizada así como el uso de la misma en elementos de prueba para detectar analitos.
MX2012001946A 2009-08-20 2010-08-18 Estabilizacion de enzimas utilizando coenzimas estables. MX2012001946A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09168327A EP2292751A1 (de) 2009-08-20 2009-08-20 Stabilisierung von Enzymen mit stabilen Coenzymen
PCT/EP2010/062045 WO2011020856A1 (de) 2009-08-20 2010-08-18 Stabilisierung von enzymen mit stabilen coenzymen

Publications (1)

Publication Number Publication Date
MX2012001946A true MX2012001946A (es) 2012-04-02

Family

ID=41211921

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012001946A MX2012001946A (es) 2009-08-20 2010-08-18 Estabilizacion de enzimas utilizando coenzimas estables.

Country Status (8)

Country Link
US (2) US9540702B2 (es)
EP (2) EP2292751A1 (es)
JP (1) JP5851401B2 (es)
KR (2) KR101554457B1 (es)
CN (1) CN102471767B (es)
CA (1) CA2770249C (es)
MX (1) MX2012001946A (es)
WO (1) WO2011020856A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5540096B2 (ja) * 2009-07-27 2014-07-02 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト カルバ−ｎａｄの酵素合成
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
US8787944B2 (en) 2011-08-18 2014-07-22 Rivada Research, Llc Method and system for providing enhanced location based information for wireless handsets
EP2636750A1 (en) 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
CN104704367A (zh) * 2012-08-09 2015-06-10 基础服务农业研究院 膜组件和包含这种膜组件的侧流免疫测定装置
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
EP2770064A1 (de) * 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
MX2016009382A (es) 2014-01-24 2016-09-16 Hoffmann La Roche Metodo de fabricacion de tiras de prueba unicodigo y sin codigo.
WO2018067235A1 (en) 2016-10-05 2018-04-12 Roche Diabetes Care, Inc. Detection reagents and electrode arrangements for multi-analyte diagnostic test elements, as well as methods of using the same
EP3339431A1 (en) * 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
EP3963058A4 (en) * 2019-05-01 2024-01-03 Codexis, Inc. MODIFIED GLUCOSE DEHYDROGENASES AND PROCESSES FOR THE REDUCTIVE AMINATION OF KETONE AND AMINO COMPOUNDS
CN114173831B (zh) * 2019-05-28 2023-11-14 W.L.戈尔及同仁股份有限公司 稳定化的医疗装置及其制造方法
AU2020337996C1 (en) * 2019-08-30 2024-05-02 Abbott Diabetes Care Inc. Analyte sensors and sensing methods for the detection of alcohol

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2061984C3 (de) 1970-12-16 1974-04-11 Boehringer Mannheim Gmbh, 6800 Mannheim Reagens zur Bestimmung der Lactat-Dehydrogenase im Farbtest
US5032506A (en) 1986-12-16 1991-07-16 Enzymatics, Inc. Color control system
US5609718A (en) 1995-09-29 1997-03-11 Micron Technology, Inc. Method and apparatus for measuring a change in the thickness of polishing pads used in chemical-mechanical planarization of semiconductor wafers
DE19543493A1 (de) * 1995-11-22 1997-05-28 Boehringer Mannheim Gmbh Stabilisierte Coenzym-Lösungen und deren Verwendung zur Bestimmung von Dehydrogenasen bzw. deren Substrate im alkalischen Milieu
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
US6710770B2 (en) * 2000-02-11 2004-03-23 Canesta, Inc. Quasi-three-dimensional method and apparatus to detect and localize interaction of user-object and virtual transfer device
WO2001094370A1 (fr) 2000-06-07 2001-12-13 Wako Pure Chemical Industries, Ltd. Derives de coenzymes et enzymes appropries
US7341830B2 (en) 2002-05-16 2008-03-11 Roche Diagnostics Operations, Inc. Method and reagent system having a non-regenerative enzyme-coenzyme complex
CA2535147A1 (en) 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
DE102006035020B4 (de) * 2005-07-28 2018-08-23 Roche Diabetes Care Gmbh Stabilisierung von NAD/NADH
DE102005035461A1 (de) 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stabile NAD/NADH-Derivate
JP4786451B2 (ja) 2005-07-28 2011-10-05 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト Ｎａｄ／ｎａｄｈの安定化
US20070196899A1 (en) 2005-11-29 2007-08-23 Sony Corporation Mutant protein having diaphorase activity
GB0625671D0 (en) 2006-12-21 2007-01-31 Oxford Biosensors Ltd Protein formulation
EP1964927A1 (de) * 2007-02-27 2008-09-03 F. Hoffmann-La Roche AG Chinone als Mediatoren für photometrische Teste
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP2287605A1 (de) * 2009-08-20 2011-02-23 Roche Diagnostics GmbH Vereinfachte Magazinierung integrierter Systeme
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
EP2333544A1 (de) * 2009-12-11 2011-06-15 F. Hoffmann-La Roche AG Sterilisierbare Chemie für Testelemente
PL2513648T3 (pl) * 2009-12-16 2015-11-30 Hoffmann La Roche Wykrywanie rozkładu enzymów w elemencie testującym przy pomocy kontrolowanego uwalniania chronionego analitu

Also Published As

Publication number Publication date
CA2770249C (en) 2015-04-28
CN102471767A (zh) 2012-05-23
KR101554457B1 (ko) 2015-09-30
EP2467478A1 (de) 2012-06-27
CN102471767B (zh) 2018-02-16
US20120276565A1 (en) 2012-11-01
KR20150070443A (ko) 2015-06-24
CA2770249A1 (en) 2011-02-24
EP2467478B1 (de) 2015-09-16
JP5851401B2 (ja) 2016-02-03
WO2011020856A1 (de) 2011-02-24
KR20120064076A (ko) 2012-06-18
US20170073661A1 (en) 2017-03-16
EP2292751A1 (de) 2011-03-09
JP2013502212A (ja) 2013-01-24
US9540702B2 (en) 2017-01-10

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