JP5746161B2 - 顕微鏡画像における蛍光の評価方法 - Google Patents

顕微鏡画像における蛍光の評価方法 Download PDF

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Publication number
JP5746161B2
JP5746161B2 JP2012516561A JP2012516561A JP5746161B2 JP 5746161 B2 JP5746161 B2 JP 5746161B2 JP 2012516561 A JP2012516561 A JP 2012516561A JP 2012516561 A JP2012516561 A JP 2012516561A JP 5746161 B2 JP5746161 B2 JP 5746161B2
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microscope
counter
image
phosphor
sample
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JP2012530947A5 (enExample
JP2012530947A (ja
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クレッペ、インゴ
カルクブレナー、トーマス
ヴォレシェンスキー、ラルフ
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2012516561A 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法 Active JP5746161B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102009031231.5 2009-06-26
DE102009031231A DE102009031231A1 (de) 2009-06-26 2009-06-26 Verfahren und Anordnungen für die Fluoreszenzmikroskopie
PCT/EP2010/003705 WO2010149319A1 (de) 2009-06-26 2010-06-19 Verfahren zum auswerten von fluoreszenzereignissen in einem mikroskopbild

Publications (3)

Publication Number Publication Date
JP2012530947A JP2012530947A (ja) 2012-12-06
JP2012530947A5 JP2012530947A5 (enExample) 2013-08-08
JP5746161B2 true JP5746161B2 (ja) 2015-07-08

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Family Applications (1)

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JP2012516561A Active JP5746161B2 (ja) 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法

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Country Link
US (1) US8988771B2 (enExample)
EP (1) EP2446314B1 (enExample)
JP (1) JP5746161B2 (enExample)
DE (1) DE102009031231A1 (enExample)
WO (1) WO2010149319A1 (enExample)

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GB201318598D0 (en) * 2013-10-21 2013-12-04 Univ Leicester Improvements in or relating to super-resolution microscopy
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
WO2019131947A1 (ja) * 2017-12-27 2019-07-04 国立研究開発法人理化学研究所 分光分析装置、分光分析方法、プログラム、記録媒体及び顕微鏡
JP7228917B2 (ja) * 2018-01-02 2023-02-27 キングス カレッジ ロンドン 局在化顕微鏡法のための方法及びシステム
US11674902B2 (en) * 2018-07-16 2023-06-13 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. System and method for use in fret microscopy
DE102018122816B9 (de) * 2018-09-18 2024-07-04 Carl Zeiss Meditec Ag Verfahren und Vorrichtung zum Bestimmen einer Eigenschaft eines Objekts
DE102018220779A1 (de) 2018-12-03 2020-06-04 Carl Zeiss Microscopy Gmbh Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe
DE102019127775A1 (de) * 2019-10-15 2021-04-15 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zur Bildaufnahme mit einem Lichtmikroskop
KR20220153579A (ko) * 2020-01-16 2022-11-18 이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디. Fret 현미경 검사에서 사용하기 위한 시스템 및 방법
JP7565554B2 (ja) * 2020-05-22 2024-10-11 国立大学法人山梨大学 分光計、分析システム及び分析方法
DE102020006975A1 (de) * 2020-11-11 2022-05-12 Carl Zeiss Microscopy Gmbh Laser-Scanning-Mikroskop und Verfahren zum Justieren eines Laser-Scanning-Mikroskops
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben
JP7865983B2 (ja) 2021-03-12 2026-05-26 ストライカー・ユーロピアン・オペレーションズ・リミテッド 腫瘍組織を検出および除去するための神経外科的方法およびシステム
CN118552949A (zh) * 2024-01-22 2024-08-27 青岛瑞斯凯尔生物科技有限公司 应用于显微镜的自动图像扫描分析方法和系统

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US8693742B2 (en) * 2008-12-17 2014-04-08 The Regents Of The University Of Colorado Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function

Also Published As

Publication number Publication date
US8988771B2 (en) 2015-03-24
WO2010149319A1 (de) 2010-12-29
EP2446314A1 (de) 2012-05-02
JP2012530947A (ja) 2012-12-06
US20120140317A1 (en) 2012-06-07
DE102009031231A1 (de) 2010-12-30
EP2446314B1 (de) 2019-10-23

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