JP5746161B2 - 顕微鏡画像における蛍光の評価方法 - Google Patents

顕微鏡画像における蛍光の評価方法 Download PDF

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Publication number
JP5746161B2
JP5746161B2 JP2012516561A JP2012516561A JP5746161B2 JP 5746161 B2 JP5746161 B2 JP 5746161B2 JP 2012516561 A JP2012516561 A JP 2012516561A JP 2012516561 A JP2012516561 A JP 2012516561A JP 5746161 B2 JP5746161 B2 JP 5746161B2
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microscope
counter
image
phosphor
sample
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JP2012530947A (ja
JP2012530947A5 (enExample
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クレッペ、インゴ
カルクブレナー、トーマス
ヴォレシェンスキー、ラルフ
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Carl Zeiss Microscopy GmbH
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
JP2012516561A 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法 Active JP5746161B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102009031231A DE102009031231A1 (de) 2009-06-26 2009-06-26 Verfahren und Anordnungen für die Fluoreszenzmikroskopie
DE102009031231.5 2009-06-26
PCT/EP2010/003705 WO2010149319A1 (de) 2009-06-26 2010-06-19 Verfahren zum auswerten von fluoreszenzereignissen in einem mikroskopbild

Publications (3)

Publication Number Publication Date
JP2012530947A JP2012530947A (ja) 2012-12-06
JP2012530947A5 JP2012530947A5 (enExample) 2013-08-08
JP5746161B2 true JP5746161B2 (ja) 2015-07-08

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Family Applications (1)

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JP2012516561A Active JP5746161B2 (ja) 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法

Country Status (5)

Country Link
US (1) US8988771B2 (enExample)
EP (1) EP2446314B1 (enExample)
JP (1) JP5746161B2 (enExample)
DE (1) DE102009031231A1 (enExample)
WO (1) WO2010149319A1 (enExample)

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WO2015046440A1 (ja) * 2013-09-27 2015-04-02 株式会社ニコン 解析装置、顕微鏡装置、解析方法、及びプログラム
GB201318598D0 (en) * 2013-10-21 2013-12-04 Univ Leicester Improvements in or relating to super-resolution microscopy
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
JPWO2019131947A1 (ja) * 2017-12-27 2020-12-24 国立研究開発法人理化学研究所 分光分析装置、分光分析方法、プログラム、記録媒体及び顕微鏡
WO2019135069A1 (en) * 2018-01-02 2019-07-11 King's College London Method and system for localisation microscopy
US11674902B2 (en) * 2018-07-16 2023-06-13 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. System and method for use in fret microscopy
DE102018122816B9 (de) * 2018-09-18 2024-07-04 Carl Zeiss Meditec Ag Verfahren und Vorrichtung zum Bestimmen einer Eigenschaft eines Objekts
DE102018220779A1 (de) 2018-12-03 2020-06-04 Carl Zeiss Microscopy Gmbh Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe
DE102019127775A1 (de) * 2019-10-15 2021-04-15 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zur Bildaufnahme mit einem Lichtmikroskop
KR20220153579A (ko) * 2020-01-16 2022-11-18 이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디. Fret 현미경 검사에서 사용하기 위한 시스템 및 방법
JP7565554B2 (ja) * 2020-05-22 2024-10-11 国立大学法人山梨大学 分光計、分析システム及び分析方法
DE102020006975A1 (de) * 2020-11-11 2022-05-12 Carl Zeiss Microscopy Gmbh Laser-Scanning-Mikroskop und Verfahren zum Justieren eines Laser-Scanning-Mikroskops
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben

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JP3616107B2 (ja) 1992-04-10 2005-02-02 株式会社日立製作所 ポリヌクレオチド検出法
US5671085A (en) 1995-02-03 1997-09-23 The Regents Of The University Of California Method and apparatus for three-dimensional microscopy with enhanced depth resolution
CN100356163C (zh) * 1996-08-16 2007-12-19 Ge保健尼亚加拉公司 用于分析井板、凝胶和斑点的数字成像系统
JP2001509612A (ja) * 1997-07-10 2001-07-24 ルプレヒト−カールス−ウニヴェルジテート ハイデルベルク ウェーブフィールド顕微鏡、ウェーブフィールド顕微鏡法、ｄｎａ順序決定のためのウェーブフィールド顕微鏡法、およびウェーブフィールド顕微鏡に対する較正方法
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JP4323369B2 (ja) 2004-04-09 2009-09-02 オリンパス株式会社 走査型蛍光顕微鏡装置
JP4673000B2 (ja) * 2004-05-21 2011-04-20 株式会社キーエンス 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器
DE102004034998A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Lichtrastermikroskop mit bewegter Lochscheibe und Verwendung
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JP5536650B2 (ja) * 2007-08-31 2014-07-02 ザ ジェネラル ホスピタル コーポレイション 自己干渉蛍光顕微鏡検査のためのシステムと方法、及び、それに関連するコンピュータがアクセス可能な媒体
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WO2009115108A1 (en) * 2008-03-19 2009-09-24 Ruprecht-Karls-Universität Heidelberg A method and an apparatus for localization of single dye molecules in the fluorescent microscopy
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DE102008021641A1 (de) * 2008-04-30 2009-11-05 Carl Zeiss Microlmaging Gmbh Auflösungsgesteigerte Lumineszenzmikroskopie
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DE102008028490A1 (de) * 2008-06-16 2009-12-17 Carl Zeiss Microimaging Gmbh Beleuchtungsanordnung für die TIRF-Mikroskopie
DE102008049886B4 (de) 2008-09-30 2021-11-04 Carl Zeiss Microscopy Gmbh Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben
DE102008054317A1 (de) 2008-11-03 2010-05-06 Carl Zeiss Microlmaging Gmbh Kombinationsmikroskopie
DE102008059328A1 (de) 2008-11-27 2010-06-02 Carl Zeiss Microimaging Gmbh Auflösungsgesteigerte Mikroskopie
US8693742B2 (en) * 2008-12-17 2014-04-08 The Regents Of The University Of Colorado Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function

Also Published As

Publication number Publication date
JP2012530947A (ja) 2012-12-06
DE102009031231A1 (de) 2010-12-30
EP2446314A1 (de) 2012-05-02
EP2446314B1 (de) 2019-10-23
US8988771B2 (en) 2015-03-24
WO2010149319A1 (de) 2010-12-29
US20120140317A1 (en) 2012-06-07

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