JP2012530947A5 - - Google Patents

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JP2012530947A5
JP2012530947A5 JP2012516561A JP2012516561A JP2012530947A5 JP 2012530947 A5 JP2012530947 A5 JP 2012530947A5 JP 2012516561 A JP2012516561 A JP 2012516561A JP 2012516561 A JP2012516561 A JP 2012516561A JP 2012530947 A5 JP2012530947 A5 JP 2012530947A5
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Japan
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microscope
counter
region
sample
phosphor
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JP2012516561A
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Japanese (ja)
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JP2012530947A (ja
JP5746161B2 (ja
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JP2012516561A 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法 Active JP5746161B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102009031231A DE102009031231A1 (de) 2009-06-26 2009-06-26 Verfahren und Anordnungen für die Fluoreszenzmikroskopie
DE102009031231.5 2009-06-26
PCT/EP2010/003705 WO2010149319A1 (de) 2009-06-26 2010-06-19 Verfahren zum auswerten von fluoreszenzereignissen in einem mikroskopbild

Publications (3)

Publication Number Publication Date
JP2012530947A JP2012530947A (ja) 2012-12-06
JP2012530947A5 true JP2012530947A5 (enExample) 2013-08-08
JP5746161B2 JP5746161B2 (ja) 2015-07-08

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JP2012516561A Active JP5746161B2 (ja) 2009-06-26 2010-06-19 顕微鏡画像における蛍光の評価方法

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US (1) US8988771B2 (enExample)
EP (1) EP2446314B1 (enExample)
JP (1) JP5746161B2 (enExample)
DE (1) DE102009031231A1 (enExample)
WO (1) WO2010149319A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012216164B4 (de) * 2012-09-12 2016-04-28 Forschungsverbund Berlin E.V. Vorrichtung mit einer Anordnung optischer Elemente
WO2015046440A1 (ja) * 2013-09-27 2015-04-02 株式会社ニコン 解析装置、顕微鏡装置、解析方法、及びプログラム
GB201318598D0 (en) * 2013-10-21 2013-12-04 Univ Leicester Improvements in or relating to super-resolution microscopy
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
JPWO2019131947A1 (ja) * 2017-12-27 2020-12-24 国立研究開発法人理化学研究所 分光分析装置、分光分析方法、プログラム、記録媒体及び顕微鏡
WO2019135069A1 (en) * 2018-01-02 2019-07-11 King's College London Method and system for localisation microscopy
US11674902B2 (en) * 2018-07-16 2023-06-13 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. System and method for use in fret microscopy
DE102018122816B9 (de) * 2018-09-18 2024-07-04 Carl Zeiss Meditec Ag Verfahren und Vorrichtung zum Bestimmen einer Eigenschaft eines Objekts
DE102018220779A1 (de) 2018-12-03 2020-06-04 Carl Zeiss Microscopy Gmbh Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe
DE102019127775A1 (de) * 2019-10-15 2021-04-15 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Verfahren zur Bildaufnahme mit einem Lichtmikroskop
KR20220153579A (ko) * 2020-01-16 2022-11-18 이슘 리서치 디벨롭먼트 컴퍼니 오브 더 히브루 유니버시티 오브 예루살렘 엘티디. Fret 현미경 검사에서 사용하기 위한 시스템 및 방법
JP7565554B2 (ja) * 2020-05-22 2024-10-11 国立大学法人山梨大学 分光計、分析システム及び分析方法
DE102020006975A1 (de) * 2020-11-11 2022-05-12 Carl Zeiss Microscopy Gmbh Laser-Scanning-Mikroskop und Verfahren zum Justieren eines Laser-Scanning-Mikroskops
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5784162A (en) 1993-08-18 1998-07-21 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for biological research, medical diagnostics and therapy
JP3616107B2 (ja) 1992-04-10 2005-02-02 株式会社日立製作所 ポリヌクレオチド検出法
US5671085A (en) 1995-02-03 1997-09-23 The Regents Of The University Of California Method and apparatus for three-dimensional microscopy with enhanced depth resolution
CN100356163C (zh) * 1996-08-16 2007-12-19 Ge保健尼亚加拉公司 用于分析井板、凝胶和斑点的数字成像系统
JP2001509612A (ja) * 1997-07-10 2001-07-24 ルプレヒト−カールス−ウニヴェルジテート ハイデルベルク ウェーブフィールド顕微鏡、ウェーブフィールド顕微鏡法、dna順序決定のためのウェーブフィールド顕微鏡法、およびウェーブフィールド顕微鏡に対する較正方法
DE19908883A1 (de) 1999-03-02 2000-09-07 Rainer Heintzmann Verfahren zur Erhöhung der Auflösung optischer Abbildung
EP1921440A3 (en) * 1999-11-12 2008-06-04 E.I. Dupont De Nemours And Company Fluorometer with low heat-generating light source
JP2005043278A (ja) 2003-07-24 2005-02-17 Institute Of Physical & Chemical Research 蛍光相関分光測定装置及び不均一系試料における拡散係数測定方法
DE10335471A1 (de) 2003-08-02 2005-03-03 Leica Microsystems Heidelberg Gmbh Detektor und Verfahren zur Detektion schwacher fluoreszenter Strahlung mit einem Mikroskopsystem
JP4323369B2 (ja) 2004-04-09 2009-09-02 オリンパス株式会社 走査型蛍光顕微鏡装置
JP4673000B2 (ja) * 2004-05-21 2011-04-20 株式会社キーエンス 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器
DE102004034998A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Lichtrastermikroskop mit bewegter Lochscheibe und Verwendung
EP1825317B1 (en) * 2004-11-24 2013-04-17 Battelle Memorial Institute Optical system for cell imaging
US7705987B2 (en) * 2005-02-15 2010-04-27 Tata Institute Of Fundamental Research Fluorescence correlation microscopy with real-time alignment readout
EP3203235A1 (en) 2005-05-23 2017-08-09 Harald F. Hess Optical microscopy with phototransformable optical labels
DE102005034443A1 (de) * 2005-07-22 2007-02-22 Carl Zeiss Jena Gmbh Auflösungsgesteigerte Lumineszenz-Mikroskopie
DE102006021317B3 (de) 2006-05-06 2007-10-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe
WO2008019299A2 (en) 2006-08-04 2008-02-14 Ikonisys, Inc. Image processing method for a microscope system
US7838302B2 (en) * 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
WO2008032096A2 (en) * 2006-09-14 2008-03-20 Oxford Gene Technology Ip Limited Apparatus for imaging single molecules
US7916304B2 (en) * 2006-12-21 2011-03-29 Howard Hughes Medical Institute Systems and methods for 3-dimensional interferometric microscopy
JP5536650B2 (ja) * 2007-08-31 2014-07-02 ザ ジェネラル ホスピタル コーポレイション 自己干渉蛍光顕微鏡検査のためのシステムと方法、及び、それに関連するコンピュータがアクセス可能な媒体
DE102008009216A1 (de) * 2008-02-13 2009-08-20 Carl Zeiss Microimaging Gmbh Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe
WO2009115108A1 (en) * 2008-03-19 2009-09-24 Ruprecht-Karls-Universität Heidelberg A method and an apparatus for localization of single dye molecules in the fluorescent microscopy
EP2107363B1 (en) * 2008-03-31 2012-07-11 Deutsches Krebsforschungszentrum Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution
US7772569B2 (en) * 2008-04-01 2010-08-10 The Jackson Laboratory 3D biplane microscopy
DE102008021641A1 (de) * 2008-04-30 2009-11-05 Carl Zeiss Microlmaging Gmbh Auflösungsgesteigerte Lumineszenzmikroskopie
US8174692B2 (en) 2008-05-21 2012-05-08 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. High spatial resolution imaging of a structure of interest in a specimen
DE102008028490A1 (de) * 2008-06-16 2009-12-17 Carl Zeiss Microimaging Gmbh Beleuchtungsanordnung für die TIRF-Mikroskopie
DE102008049886B4 (de) 2008-09-30 2021-11-04 Carl Zeiss Microscopy Gmbh Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben
DE102008054317A1 (de) 2008-11-03 2010-05-06 Carl Zeiss Microlmaging Gmbh Kombinationsmikroskopie
DE102008059328A1 (de) 2008-11-27 2010-06-02 Carl Zeiss Microimaging Gmbh Auflösungsgesteigerte Mikroskopie
US8693742B2 (en) * 2008-12-17 2014-04-08 The Regents Of The University Of Colorado Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function

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