JP5713924B2 - 活性化白血球組成物 - Google Patents
活性化白血球組成物 Download PDFInfo
- Publication number
- JP5713924B2 JP5713924B2 JP2011552537A JP2011552537A JP5713924B2 JP 5713924 B2 JP5713924 B2 JP 5713924B2 JP 2011552537 A JP2011552537 A JP 2011552537A JP 2011552537 A JP2011552537 A JP 2011552537A JP 5713924 B2 JP5713924 B2 JP 5713924B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- wound
- composition
- bag
- activated leukocyte
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000000265 leukocyte Anatomy 0.000 title claims description 101
- 239000000203 mixture Substances 0.000 title claims description 58
- 208000027418 Wounds and injury Diseases 0.000 claims description 79
- 206010052428 Wound Diseases 0.000 claims description 78
- 210000004027 cell Anatomy 0.000 claims description 63
- 239000008280 blood Substances 0.000 claims description 48
- 210000004369 blood Anatomy 0.000 claims description 47
- 238000000034 method Methods 0.000 claims description 32
- 238000011534 incubation Methods 0.000 claims description 31
- 210000003714 granulocyte Anatomy 0.000 claims description 29
- 210000002381 plasma Anatomy 0.000 claims description 29
- 210000004698 lymphocyte Anatomy 0.000 claims description 17
- 210000001616 monocyte Anatomy 0.000 claims description 17
- 230000035939 shock Effects 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 14
- 208000004210 Pressure Ulcer Diseases 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 210000002966 serum Anatomy 0.000 claims description 12
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 claims description 10
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 claims description 10
- 101001046686 Homo sapiens Integrin alpha-M Proteins 0.000 claims description 8
- 102100022338 Integrin alpha-M Human genes 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 7
- 208000014674 injury Diseases 0.000 claims description 7
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000000701 coagulant Substances 0.000 claims description 5
- 210000000822 natural killer cell Anatomy 0.000 claims description 5
- 210000000440 neutrophil Anatomy 0.000 claims description 5
- 230000008733 trauma Effects 0.000 claims description 5
- 210000003651 basophil Anatomy 0.000 claims description 4
- 230000006378 damage Effects 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 210000003979 eosinophil Anatomy 0.000 claims description 4
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 claims description 3
- 206010011985 Decubitus ulcer Diseases 0.000 claims description 3
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 claims description 3
- 210000002443 helper t lymphocyte Anatomy 0.000 claims description 3
- 210000000581 natural killer T-cell Anatomy 0.000 claims description 3
- 210000003752 saphenous vein Anatomy 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 206010002153 Anal fissure Diseases 0.000 claims description 2
- 208000016583 Anus disease Diseases 0.000 claims description 2
- 208000009531 Fissure in Ano Diseases 0.000 claims description 2
- 208000025865 Ulcer Diseases 0.000 claims description 2
- 229910001410 inorganic ion Inorganic materials 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 231100000397 ulcer Toxicity 0.000 claims description 2
- 230000002980 postoperative effect Effects 0.000 claims 2
- 208000000558 Varicose Ulcer Diseases 0.000 claims 1
- 210000003141 lower extremity Anatomy 0.000 claims 1
- 230000000065 osmolyte Effects 0.000 claims 1
- 210000001562 sternum Anatomy 0.000 claims 1
- 229940124597 therapeutic agent Drugs 0.000 claims 1
- 238000011282 treatment Methods 0.000 description 27
- 230000029663 wound healing Effects 0.000 description 19
- 210000003743 erythrocyte Anatomy 0.000 description 16
- 230000004913 activation Effects 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 9
- 238000002560 therapeutic procedure Methods 0.000 description 9
- 101001070790 Homo sapiens Platelet glycoprotein Ib alpha chain Proteins 0.000 description 7
- 102100034173 Platelet glycoprotein Ib alpha chain Human genes 0.000 description 7
- 210000001772 blood platelet Anatomy 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- 239000000306 component Substances 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 101001018097 Homo sapiens L-selectin Proteins 0.000 description 6
- 102100033467 L-selectin Human genes 0.000 description 6
- 230000000735 allogeneic effect Effects 0.000 description 6
- 239000003102 growth factor Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000002792 vascular Effects 0.000 description 6
- 108010004729 Phycoerythrin Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000020411 cell activation Effects 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 5
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 5
- 238000001802 infusion Methods 0.000 description 5
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000007170 pathology Effects 0.000 description 5
- 206010053567 Coagulopathies Diseases 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 4
- 206010018910 Haemolysis Diseases 0.000 description 4
- 108010004469 allophycocyanin Proteins 0.000 description 4
- 239000012503 blood component Substances 0.000 description 4
- 230000035602 clotting Effects 0.000 description 4
- 206010012601 diabetes mellitus Diseases 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 230000008588 hemolysis Effects 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000012552 review Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 230000037314 wound repair Effects 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 210000004207 dermis Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 210000002540 macrophage Anatomy 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 208000026350 Inborn Genetic disease Diseases 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 2
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000033115 angiogenesis Effects 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000000149 argon plasma sintering Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000001754 blood buffy coat Anatomy 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001804 debridement Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002635 electroconvulsive therapy Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000000651 myofibroblast Anatomy 0.000 description 2
- 230000017074 necrotic cell death Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000007115 recruitment Effects 0.000 description 2
- 238000011268 retreatment Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000012475 sodium chloride buffer Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 108010023728 Alloderm Proteins 0.000 description 1
- 102100035248 Alpha-(1,3)-fucosyltransferase 4 Human genes 0.000 description 1
- 108010001781 Apligraf Proteins 0.000 description 1
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 241001227713 Chiron Species 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 208000008960 Diabetic foot Diseases 0.000 description 1
- 206010056340 Diabetic ulcer Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 101001022185 Homo sapiens Alpha-(1,3)-fucosyltransferase 4 Proteins 0.000 description 1
- 101000619542 Homo sapiens E3 ubiquitin-protein ligase parkin Proteins 0.000 description 1
- 241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 206010022714 Intestinal ulcer Diseases 0.000 description 1
- 208000007766 Kaposi sarcoma Diseases 0.000 description 1
- 208000034693 Laceration Diseases 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 102000005431 Molecular Chaperones Human genes 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 108010078678 Osmolite Proteins 0.000 description 1
- 108010035766 P-Selectin Proteins 0.000 description 1
- 102100023472 P-selectin Human genes 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 208000033809 Suppuration Diseases 0.000 description 1
- 208000002847 Surgical Wound Diseases 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 206010053692 Wound complication Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000036436 anti-hiv Effects 0.000 description 1
- 238000002617 apheresis Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001815 biotherapy Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- BPKIGYQJPYCAOW-FFJTTWKXSA-I calcium;potassium;disodium;(2s)-2-hydroxypropanoate;dichloride;dihydroxide;hydrate Chemical compound O.[OH-].[OH-].[Na+].[Na+].[Cl-].[Cl-].[K+].[Ca+2].C[C@H](O)C([O-])=O BPKIGYQJPYCAOW-FFJTTWKXSA-I 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 108010015046 cell aggregation factors Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007960 cellular response to stress Effects 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 150000004683 dihydrates Chemical class 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003090 exacerbative effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 210000004475 gamma-delta t lymphocyte Anatomy 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000000495 immunoinflammatory effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 230000029774 keratinocyte migration Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000000207 lymphocyte subset Anatomy 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000011330 nucleic acid test Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000005305 organ development Effects 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 102000045222 parkin Human genes 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000001126 phototherapy Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 210000004180 plasmocyte Anatomy 0.000 description 1
- 230000010118 platelet activation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035752 proliferative phase Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008215 regulation of wound healing Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000250 revascularization Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 208000006379 syphilis Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000003106 tissue adhesive Substances 0.000 description 1
- 229940075469 tissue adhesives Drugs 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 238000011277 treatment modality Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/19—Platelets; Megacaryocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/18—Erythrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Cell Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Diabetes (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Surgery (AREA)
- Materials Engineering (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Emergency Medicine (AREA)
- Biochemistry (AREA)
- Obesity (AREA)
- Heart & Thoracic Surgery (AREA)
- General Engineering & Computer Science (AREA)
- Cardiology (AREA)
- Dermatology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20929809P | 2009-03-05 | 2009-03-05 | |
| US61/209,298 | 2009-03-05 | ||
| US21158709P | 2009-04-01 | 2009-04-01 | |
| US61/211,587 | 2009-04-01 | ||
| PCT/IB2010/000882 WO2010100570A2 (en) | 2009-03-05 | 2010-03-05 | Activated leukocyte composition |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015046833A Division JP2015129180A (ja) | 2009-03-05 | 2015-03-10 | 活性化白血球組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012519681A JP2012519681A (ja) | 2012-08-30 |
| JP2012519681A5 JP2012519681A5 (enExample) | 2013-03-28 |
| JP5713924B2 true JP5713924B2 (ja) | 2015-05-07 |
Family
ID=42710060
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011552537A Expired - Fee Related JP5713924B2 (ja) | 2009-03-05 | 2010-03-05 | 活性化白血球組成物 |
| JP2015046833A Pending JP2015129180A (ja) | 2009-03-05 | 2015-03-10 | 活性化白血球組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015046833A Pending JP2015129180A (ja) | 2009-03-05 | 2015-03-10 | 活性化白血球組成物 |
Country Status (15)
| Country | Link |
|---|---|
| EP (2) | EP2650001B1 (enExample) |
| JP (2) | JP5713924B2 (enExample) |
| KR (1) | KR101548783B1 (enExample) |
| CN (1) | CN102438635B (enExample) |
| AU (1) | AU2010220132B2 (enExample) |
| BR (1) | BRPI1006759A2 (enExample) |
| CA (2) | CA2867976A1 (enExample) |
| DK (1) | DK2403509T3 (enExample) |
| ES (1) | ES2428102T3 (enExample) |
| IL (1) | IL214953A0 (enExample) |
| MX (1) | MX2011009277A (enExample) |
| PL (1) | PL2403509T3 (enExample) |
| RU (2) | RU2015102067A (enExample) |
| WO (1) | WO2010100570A2 (enExample) |
| ZA (1) | ZA201107269B (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8574902B2 (en) | 2009-03-05 | 2013-11-05 | Macrocure Ltd. | Activated leukocyte composition and uses for wound healing |
| BR112013005610A2 (pt) * | 2010-09-09 | 2019-09-24 | Macrocure Ltd | sobrenadante condicionado de leucócito, alcs, método para o tratamento de uma ferida, método para a inibição do surgimento de infecção em uma ferida, método, sobrenadante e produto |
| WO2013136182A1 (en) | 2012-03-15 | 2013-09-19 | Macrocure, Ltd. | Activated immunostimulatory cell composition and uses thereof |
| RU2014140795A (ru) | 2012-03-15 | 2016-05-10 | Макрокьюэ, Лтд. | Активированная иммуностимулирующая клеточная композиция, используемая для лечения инфекционных заболеваний |
| EP2781224A1 (en) * | 2013-03-18 | 2014-09-24 | Khorionyx | Implantable preparations comrpsing globin insoluble at physiological pH and serum for regeneration of tissues and treatment of wounds. |
| CN107427535A (zh) | 2015-02-16 | 2017-12-01 | 纳雅康医疗有限公司 | 经修饰的血凝块 |
| GB201618106D0 (en) | 2016-10-26 | 2016-12-07 | Lift Biosciences Ltd | Cancer-killing cells |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60120821A (ja) * | 1983-12-05 | 1985-06-28 | Asahi Chem Ind Co Ltd | 悪性腫瘍治療用白血球刺激材および刺激方法 |
| US4751001A (en) * | 1984-09-24 | 1988-06-14 | Becton Dickinson And Company | Blood partitioning apparatus |
| JPH02167071A (ja) * | 1988-12-19 | 1990-06-27 | Terumo Corp | 非ヒト動物由来白血球系細胞の分離材、分離器および分離方法 |
| JPH07102151B2 (ja) * | 1994-06-03 | 1995-11-08 | 中外製薬株式会社 | 新規なポリペプチドの製造法 |
| IL110195A (en) * | 1994-07-03 | 2003-10-31 | David Danon | Method and system for cultivating macrophages |
| US6146890A (en) | 1994-07-03 | 2000-11-14 | Danon; David | Method and system for cultivating macrophages |
| JP3201610B2 (ja) * | 1995-03-17 | 2001-08-27 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 腫瘍を処置する方法 |
| JPH10259134A (ja) * | 1997-01-16 | 1998-09-29 | Sekisui Chem Co Ltd | 創傷治癒促進剤 |
| RU2143685C1 (ru) * | 1998-05-19 | 1999-12-27 | Коган Александр Харитонович | Способ дифференциального подсчета гранулоцитов, моноцитов и лимфоцитов |
| EP1731175B1 (en) * | 1998-11-12 | 2009-09-30 | International Manufacturing Group, Inc. | Hemostatic cross-linked dextran beads useful for rapid blood coagulation and hemostatis |
| AU2002353465B2 (en) * | 2001-11-21 | 2008-10-16 | Yeda Research And Development Co. Ltd. | Process for the manufacture of human mononuclear phagocytic leukocytes |
| JP4108393B2 (ja) * | 2002-07-15 | 2008-06-25 | テルモ株式会社 | 細胞増殖剤の製造方法 |
| JP4245324B2 (ja) * | 2002-10-09 | 2009-03-25 | 旭化成クラレメディカル株式会社 | 単球の分離回収方法 |
| EP2198873A1 (en) * | 2008-12-18 | 2010-06-23 | Aposcience AG | Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture |
| EP2201954A1 (en) * | 2008-12-18 | 2010-06-30 | Aposcience AG | Pharmaceutical preparation |
-
2010
- 2010-03-05 CN CN201080020134.XA patent/CN102438635B/zh not_active Expired - Fee Related
- 2010-03-05 BR BRPI1006759A patent/BRPI1006759A2/pt not_active IP Right Cessation
- 2010-03-05 EP EP13176068.8A patent/EP2650001B1/en not_active Not-in-force
- 2010-03-05 PL PL10723285T patent/PL2403509T3/pl unknown
- 2010-03-05 WO PCT/IB2010/000882 patent/WO2010100570A2/en not_active Ceased
- 2010-03-05 DK DK10723285.2T patent/DK2403509T3/da active
- 2010-03-05 RU RU2015102067/15A patent/RU2015102067A/ru not_active Application Discontinuation
- 2010-03-05 KR KR1020117023251A patent/KR101548783B1/ko not_active Expired - Fee Related
- 2010-03-05 EP EP10723285.2A patent/EP2403509B1/en not_active Not-in-force
- 2010-03-05 CA CA2867976A patent/CA2867976A1/en not_active Abandoned
- 2010-03-05 ES ES10723285T patent/ES2428102T3/es active Active
- 2010-03-05 JP JP2011552537A patent/JP5713924B2/ja not_active Expired - Fee Related
- 2010-03-05 AU AU2010220132A patent/AU2010220132B2/en not_active Ceased
- 2010-03-05 MX MX2011009277A patent/MX2011009277A/es active IP Right Grant
- 2010-03-05 CA CA2754190A patent/CA2754190A1/en not_active Abandoned
- 2010-03-05 RU RU2011139016/15A patent/RU2548741C2/ru not_active IP Right Cessation
-
2011
- 2011-09-04 IL IL214953A patent/IL214953A0/en unknown
- 2011-10-05 ZA ZA2011/07269A patent/ZA201107269B/en unknown
-
2015
- 2015-03-10 JP JP2015046833A patent/JP2015129180A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| RU2011139016A (ru) | 2013-04-10 |
| KR101548783B1 (ko) | 2015-08-31 |
| WO2010100570A3 (en) | 2010-12-16 |
| RU2548741C2 (ru) | 2015-04-20 |
| CA2754190A1 (en) | 2010-09-10 |
| JP2015129180A (ja) | 2015-07-16 |
| RU2015102067A (ru) | 2015-06-20 |
| EP2403509B1 (en) | 2013-08-21 |
| ZA201107269B (en) | 2014-03-26 |
| CA2867976A1 (en) | 2010-09-10 |
| EP2403509A2 (en) | 2012-01-11 |
| DK2403509T3 (da) | 2013-09-16 |
| WO2010100570A2 (en) | 2010-09-10 |
| KR20120002530A (ko) | 2012-01-05 |
| EP2650001A1 (en) | 2013-10-16 |
| CN102438635A (zh) | 2012-05-02 |
| BRPI1006759A2 (pt) | 2016-03-15 |
| CN102438635B (zh) | 2014-09-17 |
| AU2010220132A1 (en) | 2011-10-13 |
| AU2010220132B2 (en) | 2013-10-10 |
| IL214953A0 (en) | 2011-11-30 |
| EP2650001B1 (en) | 2016-09-07 |
| ES2428102T3 (es) | 2013-11-05 |
| MX2011009277A (es) | 2011-12-16 |
| PL2403509T3 (pl) | 2013-11-29 |
| JP2012519681A (ja) | 2012-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015129180A (ja) | 活性化白血球組成物 | |
| KR101922538B1 (ko) | 상처 치유 조성물의 제조를 위한 방법, 튜브 및 장치 | |
| US20200254019A1 (en) | Pharmaceutical preparation | |
| US9439950B2 (en) | Activated leukocyte conditioned supernatant and uses for wound healing | |
| US9132154B2 (en) | Activated leukocyte composition and uses for wound healing | |
| Zamani et al. | Blood derivatives as monotherapy and combination therapy: A promising strategy for wound healing | |
| CA3171694C (en) | Method for producing enhanced anti-inflammatory/anti-catabolic agents from autologous physiological fluid | |
| WO2016075542A1 (en) | Methods for extended storage of activated leukocyte compositions | |
| EP3784257A1 (en) | Cells differentiated from mesenchymal stem cells for the treatment of dermal lesions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130208 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20130208 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140318 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140604 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20141014 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150113 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150210 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150310 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5713924 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| LAPS | Cancellation because of no payment of annual fees |