JP5703031B2 - 核酸組換え法 - Google Patents
核酸組換え法 Download PDFInfo
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- JP5703031B2 JP5703031B2 JP2010547269A JP2010547269A JP5703031B2 JP 5703031 B2 JP5703031 B2 JP 5703031B2 JP 2010547269 A JP2010547269 A JP 2010547269A JP 2010547269 A JP2010547269 A JP 2010547269A JP 5703031 B2 JP5703031 B2 JP 5703031B2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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| Application Number | Priority Date | Filing Date | Title |
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| GBGB0803109.8A GB0803109D0 (en) | 2008-02-20 | 2008-02-20 | Method of nucleic acid recombination |
| GB0803109.8 | 2008-02-20 | ||
| PCT/IB2009/000488 WO2009104094A2 (en) | 2008-02-20 | 2009-02-20 | Method of nucleic acid recombination |
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| JP2011512153A JP2011512153A (ja) | 2011-04-21 |
| JP2011512153A5 JP2011512153A5 (https=) | 2011-12-01 |
| JP5703031B2 true JP5703031B2 (ja) | 2015-04-15 |
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| EP (1) | EP2255001B1 (https=) |
| JP (1) | JP5703031B2 (https=) |
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| CA (1) | CA2715871A1 (https=) |
| GB (1) | GB0803109D0 (https=) |
| WO (1) | WO2009104094A2 (https=) |
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| WO2011058555A1 (en) * | 2009-11-12 | 2011-05-19 | Yeda Research And Development Co. Ltd. | A method of editing dna in a cell and constructs capable of same |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| US12595500B2 (en) | 2011-09-26 | 2026-04-07 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN103945931B (zh) | 2011-09-26 | 2017-03-22 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) * | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US10407676B2 (en) | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| KR102530821B1 (ko) | 2014-12-19 | 2023-05-10 | 리제너론 파마슈티칼스 인코포레이티드 | 단일 단계 다중 표적화를 통한 표적화된 유전자 변형을 위한 방법 및 조성물 |
| WO2016196887A1 (en) * | 2015-06-03 | 2016-12-08 | Board Of Regents Of The University Of Nebraska | Dna editing using single-stranded dna |
| CN106011162A (zh) * | 2016-01-27 | 2016-10-12 | 浙江大学 | 大肠杆菌细胞内原位进化目标蛋白质的新方法 |
| US11866724B2 (en) | 2016-04-06 | 2024-01-09 | Gene Bridges Gmbh | Adenoviral vectors |
| JP6899564B2 (ja) * | 2016-08-12 | 2021-07-07 | Ｎｅｘｕｓｐｉｒａｌ株式会社 | ゲノム編集方法 |
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| US5518900A (en) * | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| AU752105B2 (en) * | 1997-12-05 | 2002-09-05 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Novel dna cloning method |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| WO2002014495A2 (en) * | 2000-08-14 | 2002-02-21 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enhanced homologous recombination mediated by lambda recombination proteins |
| US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
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- 2009-02-20 AU AU2009215361A patent/AU2009215361A1/en not_active Abandoned
Also Published As
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| WO2009104094A3 (en) | 2009-12-17 |
| WO2009104094A2 (en) | 2009-08-27 |
| EP2255001B1 (en) | 2015-03-25 |
| CA2715871A1 (en) | 2009-08-27 |
| JP2011512153A (ja) | 2011-04-21 |
| GB0803109D0 (en) | 2008-03-26 |
| CN102027112A (zh) | 2011-04-20 |
| US20110165630A1 (en) | 2011-07-07 |
| US8728820B2 (en) | 2014-05-20 |
| AU2009215361A1 (en) | 2009-08-27 |
| CN102027112B (zh) | 2013-11-06 |
| EP2255001A2 (en) | 2010-12-01 |
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