JP2011512153A5 - - Google Patents
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- Publication number
- JP2011512153A5 JP2011512153A5 JP2010547269A JP2010547269A JP2011512153A5 JP 2011512153 A5 JP2011512153 A5 JP 2011512153A5 JP 2010547269 A JP2010547269 A JP 2010547269A JP 2010547269 A JP2010547269 A JP 2010547269A JP 2011512153 A5 JP2011512153 A5 JP 2011512153A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- replacement
- stranded
- region
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 102
- 150000007523 nucleic acids Chemical class 0.000 claims 102
- 102000039446 nucleic acids Human genes 0.000 claims 102
- 238000000034 method Methods 0.000 claims 15
- 230000000295 complement effect Effects 0.000 claims 8
- 230000006798 recombination Effects 0.000 claims 7
- 238000005215 recombination Methods 0.000 claims 7
- 101100316841 Escherichia phage lambda bet gene Proteins 0.000 claims 5
- 238000006467 substitution reaction Methods 0.000 claims 5
- 238000006073 displacement reaction Methods 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 230000015556 catabolic process Effects 0.000 claims 3
- 238000006731 degradation reaction Methods 0.000 claims 3
- 230000006801 homologous recombination Effects 0.000 claims 3
- 238000002744 homologous recombination Methods 0.000 claims 3
- 230000035772 mutation Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 101150072534 sbcB gene Proteins 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0803109.8 | 2008-02-20 | ||
| GBGB0803109.8A GB0803109D0 (en) | 2008-02-20 | 2008-02-20 | Method of nucleic acid recombination |
| PCT/IB2009/000488 WO2009104094A2 (en) | 2008-02-20 | 2009-02-20 | Method of nucleic acid recombination |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011512153A JP2011512153A (ja) | 2011-04-21 |
| JP2011512153A5 true JP2011512153A5 (https=) | 2011-12-01 |
| JP5703031B2 JP5703031B2 (ja) | 2015-04-15 |
Family
ID=39272012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010547269A Expired - Fee Related JP5703031B2 (ja) | 2008-02-20 | 2009-02-20 | 核酸組換え法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8728820B2 (https=) |
| EP (1) | EP2255001B1 (https=) |
| JP (1) | JP5703031B2 (https=) |
| CN (1) | CN102027112B (https=) |
| AU (1) | AU2009215361A1 (https=) |
| CA (1) | CA2715871A1 (https=) |
| GB (1) | GB0803109D0 (https=) |
| WO (1) | WO2009104094A2 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011058555A1 (en) * | 2009-11-12 | 2011-05-19 | Yeda Research And Development Co. Ltd. | A method of editing dna in a cell and constructs capable of same |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| CN107058059B (zh) | 2011-09-26 | 2020-08-07 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) * | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US12595500B2 (en) | 2011-09-26 | 2026-04-07 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US10407676B2 (en) | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| ES2947714T3 (es) * | 2014-12-19 | 2023-08-17 | Regeneron Pharma | Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso |
| EP3303585A4 (en) * | 2015-06-03 | 2018-10-31 | Board of Regents of the University of Nebraska | Dna editing using single-stranded dna |
| CN106011162A (zh) * | 2016-01-27 | 2016-10-12 | 浙江大学 | 大肠杆菌细胞内原位进化目标蛋白质的新方法 |
| EP3440213A1 (en) | 2016-04-06 | 2019-02-13 | Gene Bridges GmbH | Adenoviral vectors |
| EP4056701A1 (en) * | 2016-08-12 | 2022-09-14 | Nexuspiral Inc. | Genome editing method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5518900A (en) * | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| US6509156B1 (en) * | 1997-12-05 | 2003-01-21 | Europaisches Laboratorium Fur Molekularoiologie (Embl) | DNA cloning method relying on the E. coli recE/recT recombination system |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| DK1311661T3 (da) * | 2000-08-14 | 2012-11-26 | Us Gov Health & Human Serv | Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner |
| US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
-
2008
- 2008-02-20 GB GBGB0803109.8A patent/GB0803109D0/en not_active Ceased
-
2009
- 2009-02-20 AU AU2009215361A patent/AU2009215361A1/en not_active Abandoned
- 2009-02-20 CA CA2715871A patent/CA2715871A1/en not_active Abandoned
- 2009-02-20 JP JP2010547269A patent/JP5703031B2/ja not_active Expired - Fee Related
- 2009-02-20 CN CN2009801137719A patent/CN102027112B/zh not_active Expired - Fee Related
- 2009-02-20 US US12/918,134 patent/US8728820B2/en not_active Expired - Fee Related
- 2009-02-20 EP EP09711877.2A patent/EP2255001B1/en not_active Not-in-force
- 2009-02-20 WO PCT/IB2009/000488 patent/WO2009104094A2/en not_active Ceased
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