CA2715871A1 - Method of nucleic acid recombination - Google Patents

Method of nucleic acid recombination Download PDF

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Publication number
CA2715871A1
CA2715871A1 CA2715871A CA2715871A CA2715871A1 CA 2715871 A1 CA2715871 A1 CA 2715871A1 CA 2715871 A CA2715871 A CA 2715871A CA 2715871 A CA2715871 A CA 2715871A CA 2715871 A1 CA2715871 A1 CA 2715871A1
Authority
CA
Canada
Prior art keywords
nucleic acid
single stranded
replacement
region
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2715871A
Other languages
English (en)
French (fr)
Inventor
Marcello Maresca
Axel Steffen Erler
Jun Fu
Philipp Martin Seibert
Adrian Francis Stewart
Youming Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bridges GmbH
Original Assignee
Gene Bridges GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Bridges GmbH filed Critical Gene Bridges GmbH
Publication of CA2715871A1 publication Critical patent/CA2715871A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2715871A 2008-02-20 2009-02-20 Method of nucleic acid recombination Abandoned CA2715871A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0803109.8 2008-02-20
GBGB0803109.8A GB0803109D0 (en) 2008-02-20 2008-02-20 Method of nucleic acid recombination
PCT/IB2009/000488 WO2009104094A2 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Publications (1)

Publication Number Publication Date
CA2715871A1 true CA2715871A1 (en) 2009-08-27

Family

ID=39272012

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2715871A Abandoned CA2715871A1 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Country Status (8)

Country Link
US (1) US8728820B2 (https=)
EP (1) EP2255001B1 (https=)
JP (1) JP5703031B2 (https=)
CN (1) CN102027112B (https=)
AU (1) AU2009215361A1 (https=)
CA (1) CA2715871A1 (https=)
GB (1) GB0803109D0 (https=)
WO (1) WO2009104094A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058555A1 (en) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) * 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
ES2947714T3 (es) * 2014-12-19 2023-08-17 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso
EP3303585A4 (en) * 2015-06-03 2018-10-31 Board of Regents of the University of Nebraska Dna editing using single-stranded dna
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
EP3440213A1 (en) 2016-04-06 2019-02-13 Gene Bridges GmbH Adenoviral vectors
EP4056701A1 (en) * 2016-08-12 2022-09-14 Nexuspiral Inc. Genome editing method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6509156B1 (en) * 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
DK1311661T3 (da) * 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms

Also Published As

Publication number Publication date
EP2255001A2 (en) 2010-12-01
EP2255001B1 (en) 2015-03-25
JP2011512153A (ja) 2011-04-21
CN102027112A (zh) 2011-04-20
WO2009104094A3 (en) 2009-12-17
JP5703031B2 (ja) 2015-04-15
US8728820B2 (en) 2014-05-20
GB0803109D0 (en) 2008-03-26
WO2009104094A2 (en) 2009-08-27
US20110165630A1 (en) 2011-07-07
CN102027112B (zh) 2013-11-06
AU2009215361A1 (en) 2009-08-27

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20140220

FZDE Discontinued

Effective date: 20160912