JP5703031B2 - 核酸組換え法 - Google Patents
核酸組換え法 Download PDFInfo
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- JP5703031B2 JP5703031B2 JP2010547269A JP2010547269A JP5703031B2 JP 5703031 B2 JP5703031 B2 JP 5703031B2 JP 2010547269 A JP2010547269 A JP 2010547269A JP 2010547269 A JP2010547269 A JP 2010547269A JP 5703031 B2 JP5703031 B2 JP 5703031B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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| GB0803109.8 | 2008-02-20 | ||
| GBGB0803109.8A GB0803109D0 (en) | 2008-02-20 | 2008-02-20 | Method of nucleic acid recombination |
| PCT/IB2009/000488 WO2009104094A2 (en) | 2008-02-20 | 2009-02-20 | Method of nucleic acid recombination |
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| JP2011512153A JP2011512153A (ja) | 2011-04-21 |
| JP2011512153A5 JP2011512153A5 (OSRAM) | 2011-12-01 |
| JP5703031B2 true JP5703031B2 (ja) | 2015-04-15 |
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| JP (1) | JP5703031B2 (OSRAM) |
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| AU (1) | AU2009215361A1 (OSRAM) |
| CA (1) | CA2715871A1 (OSRAM) |
| GB (1) | GB0803109D0 (OSRAM) |
| WO (1) | WO2009104094A2 (OSRAM) |
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| WO2011058555A1 (en) * | 2009-11-12 | 2011-05-19 | Yeda Research And Development Co. Ltd. | A method of editing dna in a cell and constructs capable of same |
| GB201009732D0 (en) | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| WO2013049227A2 (en) | 2011-09-26 | 2013-04-04 | Geneart Ag | High efficiency, small volume nucleic acid synthesis |
| WO2014153188A2 (en) * | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN115927293A (zh) | 2014-12-09 | 2023-04-07 | 生命技术公司 | 高效小体积核酸合成 |
| MX388784B (es) * | 2014-12-19 | 2025-03-20 | Regeneron Pharma | Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa. |
| CN108368502B (zh) * | 2015-06-03 | 2022-03-18 | 内布拉斯加大学董事委员会 | 使用单链dna的dna编辑 |
| CN106011162A (zh) * | 2016-01-27 | 2016-10-12 | 浙江大学 | 大肠杆菌细胞内原位进化目标蛋白质的新方法 |
| WO2017174753A1 (en) | 2016-04-06 | 2017-10-12 | Gene Bridges Gmbh | Adenoviral vectors |
| US20190284581A1 (en) * | 2016-08-12 | 2019-09-19 | Nexuspiral Inc. | Genome editing method |
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| US5518900A (en) * | 1993-01-15 | 1996-05-21 | Molecular Tool, Inc. | Method for generating single-stranded DNA molecules |
| ES2337027T3 (es) * | 1997-12-05 | 2010-04-20 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli. |
| US6355412B1 (en) * | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| AU2001283377B2 (en) * | 2000-08-14 | 2007-09-13 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Enhanced homologous recombination mediated by lambda recombination proteins |
| US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
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- 2009-02-20 US US12/918,134 patent/US8728820B2/en not_active Expired - Fee Related
- 2009-02-20 AU AU2009215361A patent/AU2009215361A1/en not_active Abandoned
- 2009-02-20 JP JP2010547269A patent/JP5703031B2/ja not_active Expired - Fee Related
- 2009-02-20 EP EP09711877.2A patent/EP2255001B1/en not_active Not-in-force
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- 2009-02-20 WO PCT/IB2009/000488 patent/WO2009104094A2/en not_active Ceased
- 2009-02-20 CN CN2009801137719A patent/CN102027112B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US8728820B2 (en) | 2014-05-20 |
| EP2255001B1 (en) | 2015-03-25 |
| CN102027112B (zh) | 2013-11-06 |
| WO2009104094A3 (en) | 2009-12-17 |
| EP2255001A2 (en) | 2010-12-01 |
| CA2715871A1 (en) | 2009-08-27 |
| US20110165630A1 (en) | 2011-07-07 |
| AU2009215361A1 (en) | 2009-08-27 |
| WO2009104094A2 (en) | 2009-08-27 |
| CN102027112A (zh) | 2011-04-20 |
| JP2011512153A (ja) | 2011-04-21 |
| GB0803109D0 (en) | 2008-03-26 |
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