JP5703031B2 - 核酸組換え法 - Google Patents

核酸組換え法 Download PDF

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Publication number
JP5703031B2
JP5703031B2 JP2010547269A JP2010547269A JP5703031B2 JP 5703031 B2 JP5703031 B2 JP 5703031B2 JP 2010547269 A JP2010547269 A JP 2010547269A JP 2010547269 A JP2010547269 A JP 2010547269A JP 5703031 B2 JP5703031 B2 JP 5703031B2
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Japan
Prior art keywords
nucleic acid
stranded
replacement
region
recombination
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Expired - Fee Related
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JP2010547269A
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English (en)
Japanese (ja)
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JP2011512153A5 (OSRAM
JP2011512153A (ja
Inventor
マレスカ,マルセーロ
エルラー,アクセル,シュテッフェン
フウ,ジュン
ザイベルト,フィリップ,マルティン
スチュワート,エイドリアン,フランシス
ザン,ヨウミン
Original Assignee
ジーン ブリッジズ ゲーエムベーハー
ジーン ブリッジズ ゲーエムベーハー
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Publication of JP2011512153A publication Critical patent/JP2011512153A/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1082Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2010547269A 2008-02-20 2009-02-20 核酸組換え法 Expired - Fee Related JP5703031B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0803109.8 2008-02-20
GBGB0803109.8A GB0803109D0 (en) 2008-02-20 2008-02-20 Method of nucleic acid recombination
PCT/IB2009/000488 WO2009104094A2 (en) 2008-02-20 2009-02-20 Method of nucleic acid recombination

Publications (3)

Publication Number Publication Date
JP2011512153A JP2011512153A (ja) 2011-04-21
JP2011512153A5 JP2011512153A5 (OSRAM) 2011-12-01
JP5703031B2 true JP5703031B2 (ja) 2015-04-15

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ID=39272012

Family Applications (1)

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JP2010547269A Expired - Fee Related JP5703031B2 (ja) 2008-02-20 2009-02-20 核酸組換え法

Country Status (8)

Country Link
US (1) US8728820B2 (OSRAM)
EP (1) EP2255001B1 (OSRAM)
JP (1) JP5703031B2 (OSRAM)
CN (1) CN102027112B (OSRAM)
AU (1) AU2009215361A1 (OSRAM)
CA (1) CA2715871A1 (OSRAM)
GB (1) GB0803109D0 (OSRAM)
WO (1) WO2009104094A2 (OSRAM)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058555A1 (en) * 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
WO2013049227A2 (en) 2011-09-26 2013-04-04 Geneart Ag High efficiency, small volume nucleic acid synthesis
WO2014153188A2 (en) * 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
MX388784B (es) * 2014-12-19 2025-03-20 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa.
CN108368502B (zh) * 2015-06-03 2022-03-18 内布拉斯加大学董事委员会 使用单链dna的dna编辑
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
WO2017174753A1 (en) 2016-04-06 2017-10-12 Gene Bridges Gmbh Adenoviral vectors
US20190284581A1 (en) * 2016-08-12 2019-09-19 Nexuspiral Inc. Genome editing method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
ES2337027T3 (es) * 1997-12-05 2010-04-20 Europaisches Laboratorium Fur Molekularbiologie (Embl) Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli.
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
AU2001283377B2 (en) * 2000-08-14 2007-09-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms

Also Published As

Publication number Publication date
US8728820B2 (en) 2014-05-20
EP2255001B1 (en) 2015-03-25
CN102027112B (zh) 2013-11-06
WO2009104094A3 (en) 2009-12-17
EP2255001A2 (en) 2010-12-01
CA2715871A1 (en) 2009-08-27
US20110165630A1 (en) 2011-07-07
AU2009215361A1 (en) 2009-08-27
WO2009104094A2 (en) 2009-08-27
CN102027112A (zh) 2011-04-20
JP2011512153A (ja) 2011-04-21
GB0803109D0 (en) 2008-03-26

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