JP5694342B2 - 複数の蛍光信号を監視する装置 - Google Patents
複数の蛍光信号を監視する装置 Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
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Description
Claims (21)
- 複数の蛍光信号源からの蛍光信号を検出する検出器であって、
前記複数の蛍光信号源からの前記蛍光信号を個別に検出する複数のピクセルであって、各ピクセルが、受け取られた前記蛍光信号を検出し、検出信号を生成するために、予め決められた数の少なくとも2つの検出素子を有する、複数のピクセルと、
前記少なくとも2つの検出素子から前記検出信号を受け取り、前記少なくとも2つの検出素子のうちどれが最も強い検出信号を生成したかを示すピクセル出力信号を生成する信号変換回路であって、ウィナーテイクオール回路を有する信号変換回路と、
を有する検出器。 - 各ピクセルが、少なくとも4の検出素子を有する、請求項1に記載の検出器。
- 各ピクセルが8乃至16の検出素子を有する、請求項1に記載の検出器。
- 前記信号変換回路がデジタルのピクセル出力信号を生成する、請求項1に記載の検出器。
- 前記複数のピクセルが、列及び行に沿ったアレイとして配され、前記検出器が更に、前記ピクセルを個別にアドレッシングし、前記ピクセルから前記ピクセル出力信号を読み出すアドレッシング及び読み出し手段を有する、請求項1に記載の検出器。
- 前記アドレッシング及び読み出し手段が、各検出素子ごとに選択スイッチを有し、前記選択スイッチは、関連する検出素子によって生成された出力信号を、関連する信号変換回路に送ることを可能にするために、選択スイッチアドレッシング信号を用いてオン及びオフを切り替えられることができる、請求項5に記載の検出器。
- 前記選択スイッチがN型MOSFETトランジスタである、請求項6に記載の検出器。
- 前記アドレッシング及び読み出し手段が、各検出素子ごとにリセットスイッチを有し、前記リセットスイッチは、各検出期間の後に前記検出素子をリセットするために、リセット信号を用いてオン及びオフを切り替えられることができる、請求項5に記載の検出器。
- 前記リセットスイッチがN型MOSFETトランジスタである、請求項8に記載の検出器。
- 前記アドレッシング及び読み出し手段が、各検出素子ごとに、前記検出素子の検出信号を検出器電流信号に変換する電圧電流変換素子を有する、請求項5に記載の検出器。
- 前記電圧電流変換素子は、そのゲートが関連する検出素子の出力に結合されるP型MOSFETトランジスタを有する、請求項10に記載の検出器。
- 前記ウィナーテイクオール回路は、接続される各検出素子ごとに、
ドレイン端子が、前記検出素子によって検出される検出信号を表す検出器電流信号を供給され、ソース端子が基準電位に結合され、ゲート端子が、予め決められたバイアス電流を供給される、第1のN型MOSFETトランジスタと、
ゲート端子が、前記第1のN型MOSFETトランジスタのドレイン端子に結合され、ソース端子が、前記第1のN型MOSFETトランジスタのゲート端子に結合されて、前記予め決められたバイアス電流を供給され、ドレイン端子が、検出器素子出力信号を出力する、第2のN型MOSFETトランジスタと、
を有する、請求項1に記載の検出器。 - 前記基準電位がグランド電位である、請求項12に記載の検出器。
- 前記ウィナーテイクオール回路が、前記複数のピクセルのエリア外に配される、請求項1に記載の検出器。
- 前記ウィナーテイクオール回路が、前記複数のピクセルのエリア内に配される、請求項1に記載の検出器。
- 前記ウィナーテイクオール回路が、関連するピクセルのエリア内に配される、請求項1に記載の検出器。
- 各ピクセルごとに、2つのウィナーテイクオール回路が関連付けられ、
第1のウィナーテイクオール回路が、前記複数のピクセルのエリア外に配され、第2のウィナーテイクオール回路が、前記複数のピクセルのエリア内に配される、請求項1に記載の検出器。 - 各ピクセルごとに、2つのウィナーテイクオール回路が関連付けられ、
第1のウィナーテイクオール回路が、前記複数のピクセルのエリア外に配され、第2のウィナーテイクオール回路が、関連するピクセルのエリア内に配される、請求項1に記載の検出器。 - 複数の別個の蛍光信号を監視する装置であって、
複数の別個の蛍光信号源をその上に有する基板と、
励起照明源と、
前記複数の蛍光信号源からの蛍光信号を検出する検出器と、
前記励起照明源からの励起照明を、前記基板上の前記複数の別個の蛍光信号源に同時に導くとともに、前記複数の蛍光信号源からの蛍光信号を前記検出器に導くように位置付けられる光学トレインと、
を有し、前記検出器が、
前記複数の蛍光信号源からの前記蛍光信号を個別に検出する複数のピクセルであって、各ピクセルが、受け取られた前記蛍光信号を検出し、検出信号を生成するために、予め決められた数の少なくとも2つの検出素子を有する、複数のピクセルと、
前記少なくとも2つの検出素子から前記検出信号を受け取り、前記少なくとも2つの検出素子のうちどれが最も強い検出信号を生成したかを示すピクセル出力信号を生成する信号変換回路であって、ウィナーテイクオール回路を有する信号変換回路と、
を有する装置。 - 前記光学トレインが、
前記基板上の前記複数の蛍光信号源からの蛍光信号を同時に収集するために、前記基板の第1の焦点面に焦点をあわせられる対物レンズと、
前記蛍光信号のスペクトル成分を空間的に分離するスペクトル分離手段と、
前記蛍光信号の空間的に分離されたスペクトル成分を受け取り、それらを前記検出器に集束させる集束レンズと、
を有する、請求項19に記載の装置。 - 蛍光標識化されたヌクレオチドを用いるDNAシークエンシングのために使用される、請求項19に記載の装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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EP09173971 | 2009-10-23 | ||
EP09173971.4 | 2009-10-23 | ||
PCT/IB2010/054709 WO2011048539A1 (en) | 2009-10-23 | 2010-10-18 | Device for monitoring a plurality of discrete fluorescence signals |
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JP2013508718A JP2013508718A (ja) | 2013-03-07 |
JP5694342B2 true JP5694342B2 (ja) | 2015-04-01 |
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US (1) | US9228947B2 (ja) |
EP (1) | EP2491370B1 (ja) |
JP (1) | JP5694342B2 (ja) |
KR (1) | KR20120101396A (ja) |
CN (1) | CN102575991B (ja) |
BR (1) | BR112012009260A2 (ja) |
RU (1) | RU2543429C2 (ja) |
WO (1) | WO2011048539A1 (ja) |
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JP5772425B2 (ja) | 2011-09-13 | 2015-09-02 | ソニー株式会社 | 微小粒子測定装置 |
JP6015735B2 (ja) * | 2014-11-07 | 2016-10-26 | ソニー株式会社 | 微小粒子測定装置 |
FR3048316B1 (fr) * | 2016-02-29 | 2019-06-28 | Sagem Defense Securite | Dispositif de detection d'un spot laser |
US20180052106A1 (en) * | 2016-08-17 | 2018-02-22 | Kerry Gunning | Dual detection scheme for dna sequencing |
JP2018163162A (ja) * | 2018-06-04 | 2018-10-18 | ソニー株式会社 | 微小粒子測定装置 |
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JP3176821B2 (ja) * | 1995-05-24 | 2001-06-18 | シャープ株式会社 | 入力判定回路 |
JPH1123532A (ja) * | 1997-07-01 | 1999-01-29 | Hitachi Electron Eng Co Ltd | 蛍光検出装置 |
US6046466A (en) * | 1997-09-12 | 2000-04-04 | Nikon Corporation | Solid-state imaging device |
US6992709B1 (en) * | 1999-02-26 | 2006-01-31 | Intel Corporation | Method and apparatus for high-speed broadband illuminant discrimination |
AU4061200A (en) * | 1999-03-31 | 2000-10-16 | Regents Of The University Of California, The | Multi-channel detector readout method and integrated circuit |
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JP4897164B2 (ja) * | 2001-09-27 | 2012-03-14 | 三井造船株式会社 | 電子パルス検出装置および電子パルス検出チップ |
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AU2003290429A1 (en) | 2002-12-25 | 2004-07-22 | Casio Computer Co., Ltd. | Optical dna sensor, dna reading apparatus, identification method of dna and manufacturing method of optical dna sensor |
JP4103581B2 (ja) * | 2002-12-25 | 2008-06-18 | カシオ計算機株式会社 | Dna読取装置及びdnaの同定方法 |
JP4232656B2 (ja) * | 2004-03-02 | 2009-03-04 | カシオ計算機株式会社 | 蛍光検出チップ |
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JP2008209268A (ja) * | 2007-02-27 | 2008-09-11 | Casio Comput Co Ltd | 生体高分子分析装置 |
EP2150806A4 (en) | 2007-05-10 | 2013-01-02 | Pacific Biosciences California | METHODS AND SYSTEMS FOR ANALYZING FLUORESCENT MATERIAL WITH LIMITED AUTOFLUORESCENCE |
RU2339953C1 (ru) * | 2007-06-13 | 2008-11-27 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт биологического приборостроения" (ФГУП "ГосНИИ БП") | Способ многоаналитного иммуноанализа с использованием микрочастиц |
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- 2010-10-18 WO PCT/IB2010/054709 patent/WO2011048539A1/en active Application Filing
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- 2010-10-18 KR KR1020127013154A patent/KR20120101396A/ko not_active Application Discontinuation
- 2010-10-18 CN CN201080047694.4A patent/CN102575991B/zh active Active
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BR112012009260A2 (pt) | 2019-09-24 |
EP2491370A1 (en) | 2012-08-29 |
RU2012121143A (ru) | 2013-11-27 |
EP2491370B1 (en) | 2023-03-01 |
WO2011048539A1 (en) | 2011-04-28 |
JP2013508718A (ja) | 2013-03-07 |
CN102575991B (zh) | 2016-06-15 |
RU2543429C2 (ru) | 2015-02-27 |
KR20120101396A (ko) | 2012-09-13 |
US9228947B2 (en) | 2016-01-05 |
CN102575991A (zh) | 2012-07-11 |
US20120208263A1 (en) | 2012-08-16 |
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