JP5671051B2 - 多孔質基材上で試料を処理する方法及びシステム - Google Patents
多孔質基材上で試料を処理する方法及びシステム Download PDFInfo
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Description
図1〜図3に示すように、全体を装置10として示す本発明の圧迫装置の一実施形態は、例えば頂部シールプラグ14及び底部シールプラグ18を含み得る圧迫機を含んでおり、これら頂部及び底部プラグは、それぞれ、分離領域を画成する開口を有し、流体の通過を可能にする流体入口及び流体出口、又はチャンネル15及び19を有する。この例のプラグのチャンネルはサイズが適合しており、頂部及び底部プラグが互いに接触していればこれら2つの部分を通って妨害なく流体が流れるように並んでいる。この非限定的実施形態では、チャンネルを通る流れは2つの部分の分割線、すなわち分離領域において直径3mmの円形の断面を有する。チャンネルは、例えば図2に示すように、2つの部分の分割線の上方及び下方で断面が狭くなり得る。中空のプラグは分割線のところで、この非限定例の場合、0.0139in2(9mm2)のシール面42を有していて、直径3mmの断面を有する分離領域46を形成している。この例では、フランジレスチュービングナット22(フェルールは図に示してない)を用いて、外径1/16”及び内径1mmを有するチュービングを支持しており、緩衝液のような流体を流体入口26及び出口28を介して輸送する。分離領域46は3mm(矢印C)又は円形の断面に限定されない。分離領域の大きさと形状は装置の所定の応用又は用途に応じて変化し得る。
多孔質基材上で試料を処理する本発明の1つの方法の一例は、多孔質基材(乾燥した分析物を含有している)を装置のスロット内に入れて、シールプラグのヘッドが所望の抽出領域と並ぶようにした後、(プッシャーを介して)シールプラグに力をかけることを含んでいる。シールプラグのヘッドがペーパーを圧迫して、流体入口を介して分離領域に導入される抽出緩衝液のような液体が、基材に緩衝液が適用される最初の点から外へ吸い上げられるのを防ぐシールを形成する。緩衝液は、この例では、第1のプッシャーに同心に配置された中空のボア内に設置された入口管を介して基材に適用される。この緩衝液は、ペーパーを貫通して、分離領域の外へ吸い上げられることなく流れ、次にこの例では底部、すなわち第2のプッシャー内に同心に設置された中空のボア内に設置された出口管を通って、ウェルプレート又はバイアルのような受容器内へ流れる。一方又は両方のプッシャー(シールプラグ)が他方に向かって移動し得るか、又は一方が静止していて他方のプッシャーのみがその静止プッシャーに向かって移動し得る。この例において流体はペーパーに対して垂直な方向に流れる。1以上の抽出の後、試料及び/又は抽出流体と接触する装置の表面を、清浄化又はその他の方法で物質を除いて、交差汚染を低減又は抑制し得る。例えば、空気を装置又はシステム中に導入し強制的に通して、圧迫力をかけたままで流体通路内のあらゆる残りの液体又は異物を除去し得る。また、空気を導入して試料領域から過剰の流体を除去して、その位置を乾燥すると共に圧迫力が解放された後の流体の吸い上げを防ぐこともできる。その後、シールプラグから圧迫力を解放し、ペーパーをスロットから取り出す。また、クリアリングステップはペーパーを取り出し、圧迫力を再度かけて流体通路を装置又はシステムの様々な構成要素に再度接続した後に行うか、又は繰り返してもよい。
例えば図8に示すシステムのようなシステムと関連して使用されるプロセスの非限定例は、種々様々に以下の作動を含み得る。ウェルプレートがプレート積み重ね機から移される。バイアルストリップがウェルプレートシャトルから取り出され、バイアルストリップ上のバイアルが試料処理サブシステム(例えば81)の流体通路内に配置される。自動アセンブリ(例えば98)がマガジンカセットからFTAカードを取り出し、そのFTAカードをカメラの画像化領域内に移動する。画像化システムがFTAカード上の1以上の試料の位置を決定する。次に、自動アセンブリがFTAカードを圧迫装置の分離領域及び流体流通路内に配置する。圧迫装置のアクチュエーターが伸びてFTAペーパーを圧迫する。バイアルの1つを上昇させてバイアルのカバーに穴を開ける。次に、溶媒がポンプにより、マニフォルドから圧迫装置の流体入口を介して流され、FTAカード上の試料の分離領域を通り、試料から抽出された物質と共に受容器バイアル中に入る。同一のシステム内に複数の自動化アセンブリがあってもよい。
Claims (4)
- 多孔質基材(24;48;70)に固定された試料を処理するシステムであって、
複数の試料処理サブシステムであって、その各々が、
1以上の流体分離領域(46;49)を画成する圧迫機、
圧迫機の流体分離領域に対応する開口を有する、多孔質基材のための支持体、
圧迫機の少なくとも一部分を多孔質基材に押し付けるアクチュエーター(12;54)、
少なくとも圧迫機が多孔質基材を押圧しているとき流体分離領域へのアクセス通路を有する流体入口(26)、及び
少なくとも圧迫機が多孔質基材を押圧しているとき、圧迫機の流体分離領域に対応する支持体内の開口を介して流体を受容する流体出口(28)
を含んでいる複数の試料処理サブシステムと、
試料処理サブシステムの流体入口と流体連通したマニフォルド(58;82)と、
複数の多孔質基材を収容するためのカセット(94)と、
1以上の流体受容器を試料処理サブシステムに輸送するための受容器シャトルサブシステムと、
多孔質基材をカセットから試料処理サブシステムに輸送するための自動アセンブリと、
試料処理サブシステム及び自動アセンブリを調和させるコントローラー(102)と、
多孔質基材上の試料を画像化するための画像化サブシステムと
を含んでなる、システム。 - 支持体上の多孔質基材(24;48;70)に固定された試料を処理するための方法であって、当該方法が、
多孔質基材上に圧迫シール(42)を創成して、分離ゾーン(46;49)を形成することにより、その内部に試料の一部分を分離するステップと、
分離ゾーン内に分離された試料に流体を適用して、試料表面に対して実質的に垂直な方向に試料を通って流体を移動させるステップと、
分離ゾーンに適用された後流体の少なくとも一部分を収集するステップと、
収集された流体及び分離ゾーン内の試料の一部分の一方又は両方を分析するステップと、
圧迫シールを創成する前の多孔質基材上の試料を画像化するステップと
を含んでおり、圧迫シールを創成するステップが多孔質基材上に複数の分離ゾーンを形成することを含む、方法。 - さらに、分離ゾーンと接触して残留する流体を除くステップを含む、請求項2記載の方法。
- さらに、流体を収集した後に試料を画像化するステップを含む、請求項2又は請求項3記載の方法。
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