JP5668001B2 - Skin cosmetics and hair cosmetics - Google Patents

Description

  The present invention relates to an anti-inflammatory agent, an anti-aging agent, a hair-restoring agent and an anti-androgen agent comprising a plant extract and / or an acid hydrolyzate of the extract as an active ingredient, and a plant extract and / or the extract. The present invention relates to a skin cosmetic and a hair cosmetic containing an acid hydrolyzate.

  There are various causes and onset mechanisms of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, and other skin diseases with rough skin. In addition, a tumor necrosis factor (hereinafter referred to as “TNF-α”) produced from macrophages is known.

  TNF-α was found as a factor that necrotizes tumors, but is recently considered to be a cytokine that plays a mediator role not only for tumors but also for regulating the function of normal cells. TNF-α plays an important role in the process from the onset to the end of inflammation, but its continuous and excessive production causes damage to tissues including the skin, and causes systemic fever and cachexia. And cause worsening of inflammation. As such inflammation, for example, chronic inflammatory diseases such as rheumatoid arthritis and osteoarthritis are representative. Therefore, it is important to suppress excessive production of TNF-α in pathological inflammation. As a plant extract having a TNF-α production inhibitory effect, an extract from a convolvulaceae yosai (see Patent Document 1) and the like are known.

  As other causes for causing inflammatory diseases, hyaluronidase activation, histamine release, platelet aggregation and the like are known. The activation of hyaluronidase promotes histamine degranulation from mast cells, resulting in inflammation. Therefore, prevention, treatment or improvement of inflammatory diseases can be expected by inhibiting the activation of hyaluronidase. As a plant extract having hyaluronidase inhibitory activity, an extract from Japanese maple (see Patent Document 2) and the like are known.

  Further, since hexosaminidase is released at the same time as histamine is released, the histamine release inhibitory action can be evaluated using the release of hexosaminidase as an index. Therefore, it is considered that by suppressing the release of hexosaminidase, the release of histamine can also be suppressed at the same time, which is effective for preventing, treating or improving inflammatory diseases. As a plant extract having a hexosaminidase release inhibitory action, an extract from Fuji tea (Patent Document 3) and the like are known.

  Platelet aggregates and activates to cause hemostasis physiologically and pathologically thrombus formation, and platelet aggregation is involved in the progression of arteriosclerosis, cancer metastasis, inflammation, etc. It is considered. Therefore, prevention, treatment or improvement of the above diseases can be expected by suppressing platelet aggregation. As a plant extract having a platelet aggregation inhibitory action, an extract from lichen snow tea (see Patent Document 4) and the like are known.

  One cause of skin aging with aging is a decrease in the secretion of estrogen, a female hormone. In other words, estrogen is deeply involved in maintaining the health of adult women, and its lack of secretion leads to various medical illnesses, as well as the cause of unwanted skin changes such as skin irritability, reduced elasticity, reduced moisture, etc. It is known to be. Therefore, a drug containing a substance having the same action as estrogen is transdermally or orally administered to women after menopause, whose estrogen secretion declines. As a plant extract having an estrogen-like action, an extract from pentagon (see Patent Document 5) and the like are known.

  Skin structure is roughly divided into epidermis, basement membrane, dermis and subcutaneous tissue. The basement membrane exists at the boundary between the epidermis and the dermis, and its functions are diverse. Adhesion of the epidermis to the dermis, determination of the polarity of the epidermis, control of epidermal differentiation and proliferation, and production of dermal cells It is involved in the regulation of nutrient supply derived from factors and blood components. Therefore, the basement membrane plays an extremely important role in maintaining the structure and homeostasis of the skin. Therefore, when the structure of the basement membrane changes, skin aging symptoms such as wrinkles and sagging appear. In particular, when the amount of type IV collagen, which is the main component of the basement membrane, decreases, the structure of the basement membrane changes, resulting in skin aging symptoms such as wrinkles and sagging. Known plant extracts having a type IV collagen production promoting action include extracts derived from plants and animals selected from the group consisting of hydrolyzed casein, beech buds, erythrina, soluble eggshell membranes, cuckoo, and western ivy. (See Patent Document 6).

  When the skin is irradiated with ultraviolet rays, the cells of the skin are damaged or cell death is caused, the skin loses its elasticity and elasticity, and exhibits aging symptoms such as rough skin and wrinkles. Therefore, prevention or improvement of skin aging can be expected by suppressing / recovering damage (for example, cell damage, cell death, etc.) caused by ultraviolet irradiation. Oil-soluble licorice extracts (see Patent Document 7) and the like are known as plant extracts having a damage recovery action by ultraviolet irradiation.

  The epidermis acts to alleviate external stimuli and control the loss of body components such as moisture, and is composed of a basal layer, a spiny layer, a granular layer, and a stratum corneum. Cells that divide and proliferate in the basal layer differentiate while passing through the spiny layer and granule layer, and become a stratum corneum composed of keratin protein fibers with strong cross-linking, and finally, the stratum corneum as plaque Drop off from. In particular, in the granular layer, the cell membrane is thickened to form a thickened cell membrane, and between the protein molecules is glutamyl-lysine cross-linked by the action of transglutaminase-1 to form a strong keratin protein fiber. Furthermore, ceramide or the like is covalently bonded to a part of the ceramide to form a hydrophobic structure, thereby providing a foundation for the lamellar structure of the intercellular lipid and forming the basis of the keratin barrier function.

  However, when the amount of transglutaminase-1 produced in the epidermis decreases with aging, the keratin barrier function and the skin moisturizing function decrease, and skin aging symptoms such as rough skin and dry skin come to be exhibited. Therefore, it is considered that aging symptoms of skin can be prevented or improved by promoting production of transglutaminase-1 in the epidermis. Based on such an idea, bittern or calcium chloride which is a constituent component thereof is known as having transglutaminase-1 production promoting action (see Patent Document 8).

  Many steroid hormones are expressed in the form of molecules secreted from production organs and bind to receptors to exert their effects. In the case of male hormones collectively called androgens, for example, testosterone enters the cells of target organs and becomes testosterone. After being reduced to 5α-dihydrotestosterone (5α-DHT) by 5α-reductase, it binds to the receptor and develops an action as an androgen.

  Androgen is an important hormone, but when it works excessively, it is variously preferred, such as androgenetic alopecia, hirsutism, seborrhea, acne (such as acne), benign prostatic hyperplasia, prostate tumor, premature boyhood Not triggering symptoms. Therefore, conventionally, in order to improve these various symptoms, a method of suppressing the action of excess androgen, specifically, by inhibiting the action of testosterone 5α-reductase that reduces testosterone to active 5α-DHT, There have been proposed a method for suppressing the generation of active 5α-DHT and a method for preventing the expression of androgen activity by inhibiting the binding of 5α-DHT generated from testosterone to the receptor.

  As a plant extract having such a testosterone 5α-reductase inhibitory action, for example, an extract from a monopod of the genus Ligaceae (see Patent Document 9) is known. Moreover, as a plant extract which has the effect | action which inhibits the coupling | bonding of 5 (alpha) -DHT and its receptor, the extract (refer patent document 10) etc. from a majito and / or a cuture is known, for example.

JP 2004-250344 A JP 2003-1113068 A JP 2003-12532 A JP 2005-82532 A JP 2002-302452 A JP 2004-18471 A JP 2004-250368 A JP 2004-51596 A JP-A-2005-75786 JP 2002-241297 A

  The present invention relates to an anti-inflammatory agent, an anti-aging agent, a hair-restoring agent and an anti-androgen agent comprising a highly safe natural extract and / or acid hydrolyzate thereof as an active ingredient, and the natural extract and / or acid thereof. It aims at providing the skin cosmetics and hair cosmetics which mix | blended the hydrolyzate.

  In order to achieve the above object, the anti-inflammatory agent, anti-aging agent, hair-restoring agent or anti-androgen agent of the present invention contains an extract from Hunan coffee tea and / or an acid hydrolyzate of the extract as an active ingredient. The skin cosmetic or hair cosmetic of the present invention is characterized by blending an extract from Hunan coffee tea and / or an acid hydrolyzate of the extract.

  In the anti-inflammatory agent of the present invention, the extract and the acid hydrolyzate are selected from the group consisting of a TNF-α production inhibitory action, a hyaluronidase inhibitory action, a hexosaminidase release inhibitory action, and a platelet aggregation inhibitory action. It is preferable to have seeds or two or more actions. In the anti-aging agent of the present invention, the extract has a damage recovery action by ultraviolet irradiation, and the acid hydrolyzate has an estrogen-like action, a type IV collagen production promoting action, a damage by ultraviolet irradiation. It preferably has one or more actions selected from the group consisting of a recovery action and a transglutaminase-1 production promoting action. Furthermore, in the hair restorer or anti-androgen hormone agent of the present invention, the extract has a testosterone 5α-reductase inhibitory action and / or an androgen receptor binding inhibitory action, and the acid hydrolyzate contains testosterone 5α. -It preferably has a reductase inhibitory action.

  Here, “androgen receptor binding inhibition” in the present invention means inhibition of binding between 5α-DHT and androgen receptor, and the inhibition mode is not particularly limited. For example, competitive antagonists Inhibition as an antagonist, such as a non-competitive antagonist, is contemplated. Further, in the present invention, “damage due to ultraviolet irradiation” means skin cell damage due to ultraviolet irradiation. Cell damage includes damage at the tissue level and organ level as well as damage at the cell level. Disorders are included, and examples include cell death, wrinkle formation, reduced skin elasticity, reduced skin barrier function, reduced stratum corneum moisturizing function, and the like.

  According to the present invention, an extract from a natural product, Hunan 甜 tea, and / or an acid hydrolyzate of the extract as an active ingredient, a highly safe anti-inflammatory agent, anti-aging agent, hair restorer, Male hormones, skin cosmetics and hair cosmetics can be provided.

The present invention will be described below.
[Anti-inflammatory agent, anti-aging agent, hair restorer, anti-androgen agent]
The anti-inflammatory agent, anti-aging agent, hair-restoring agent, or anti-androgen agent of the present invention contains an extract from Hunan coffee tea and / or an acid hydrolyzate of the extract as an active ingredient.

  Here, in the present invention, the “extract” refers to an extract obtained by using Hunan coffee tea (scientific name: Lithocarpus litseifolius) as an extraction raw material, a diluted or concentrated solution of the extract, and a dry product obtained by drying the extract. Or any of these crudely purified products or purified products.

  The extraction raw material used in the present invention is Hunan 甜 tea (scientific name: Lithocarpus litseifolius). Hunan tea (Lithocarpus litseifolius) is a plant belonging to the family Beechaceae and is widely distributed in the southwestern region of China, the southern region of China, etc., and can be easily obtained from these regions. The constituent parts of the Konan tea used as the raw material for extraction are not particularly limited, and examples thereof include flower parts, leaf parts, branch parts, fruit parts, bark, root parts, etc., among these, especially leaf parts or branches. It is preferred to use parts or mixtures thereof.

  Details of substances with anti-inflammatory action, anti-aging action, hair-growth action or anti-androgenic action contained in the extract from Hunan coffee tea are unknown, but depending on the extraction method commonly used for plant extraction In addition, an extract having an anti-inflammatory action, an anti-aging action, a hair-growth action or an anti-androgen action can be obtained from Hunan 甜 tea.

  For example, it can be obtained by drying the extraction raw material, pulverizing the raw material as it is or using a crusher, and subjecting it to extraction with an extraction solvent. At this time, the extraction raw material may be dried in the sun or by a commonly used dryer.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These may be used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferred.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a liquid mixture of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by weight of a lower aliphatic alcohol with respect to 10 parts by weight of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by mass of a lower aliphatic ketone with 10 parts by mass of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by mass of polyhydric alcohol with respect to parts by mass.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. The obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method in order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or a purified product thereof. Processing may be performed.

  Purification can be performed by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like. The obtained extract can be used as it is as an active ingredient of an anti-inflammatory agent, an anti-aging agent, a hair-restoring agent, or an anti-androgen hormone, but a concentrated solution or a dried product is easier to use.

  Since the extract from Hunan coffee tea has a characteristic odor, it can be purified for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in its physiological activity. Since it is not used in a large amount when blended into hair cosmetics, there is no practical problem even if it is not purified.

  The method for obtaining the acid hydrolyzate of the extract from the extract from Hunan 甜 tea obtained as described above is not particularly limited, and specifically, it can be obtained as follows. .

  A mixed solvent of an acid and a hydrophilic organic solvent is added to the extract from Hunan coffee tea, and the mixture is reacted under reflux heating. The obtained reaction product is subjected to column chromatography packed with a porous adsorbent, and water. The lower aliphatic alcohol having 1 to 5 carbon atoms (eg, methanol, ethanol, etc.) is eluted in this order to obtain a fraction of the lower aliphatic alcohol. And the acid hydrolyzate of Hunan persimmon tea extract can be obtained by distilling a solvent off from the obtained fraction.

  The acid in the mixed solvent is not particularly limited, and for example, hydrochloric acid, sulfuric acid, nitric acid, acetic acid and the like can be used. Further, the hydrophilic organic solvent in the mixed solvent is not particularly limited, and for example, methanol, ethanol, butanol, isopropanol, acetonitrile and the like can be used.

  Since the Hunan coffee tea extract or acid hydrolyzate of the extract obtained as described above has an anti-inflammatory action, an anti-aging action, a hair growth action or an anti-androgen action, each action is used. Thus, it can be used as an active ingredient of an anti-inflammatory agent, an anti-aging agent, a hair-restoring agent or an anti-androgenic agent. In that case, they may be used alone or in combination. In the case of using a mixture of Hunan 甜 tea extract and the acid hydrolyzate of the extract, the mixing ratio is not particularly limited.

  The anti-inflammatory action of the Hunan coffee tea extract or the acid hydrolyzate of the extract is, for example, from the group consisting of TNF-α production inhibitory action, hyaluronidase activity inhibitory action, hexosaminidase release inhibitory action, and platelet aggregation inhibitory action It is exhibited based on one or more selected actions. However, the anti-inflammatory action which Hunan coffee tea extract or the acid hydrolyzate of the said extract has is not limited to the anti-inflammatory action exhibited based on these actions.

  The anti-aging effect of the Hunan coffee tea extract is demonstrated based on, for example, the damage recovery effect by ultraviolet irradiation, and the anti-aging action of the acid hydrolyzate of the extract is, for example, estrogenic action, type IV collagen production It is exhibited based on one or more actions selected from the group consisting of a promoting action, a damage recovery action by ultraviolet irradiation, and a transglutaminase-1 production promoting action. However, the anti-aging action of the Hunan 甜 tea extract or the acid hydrolyzate of the extract is not limited to the anti-aging action exhibited based on these actions.

  The hair-growth action of the Hunan coffee tea extract is exhibited based on, for example, testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action. The hair-growth action of the acid hydrolyzate of the extract is, for example, testosterone It is exhibited based on the 5α-reductase inhibitory action. However, the hair-restoring action of the Hunan coffee tea extract or the acid hydrolyzate of the extract is not limited to the hair-restoring action exhibited based on these actions.

  The antiandrogenic action of the Hunan coffee extract is exhibited based on, for example, testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action, and the antiandrogen action of the acid hydrolyzate of the extract is For example, it is exhibited based on testosterone 5α-reductase inhibitory action. However, the antiandrogen action which Hunan coconut tea extract or the acid hydrolyzate of the said extract has is not limited to the antiandrogen action which is exhibited based on these actions.

  In addition, Hunan 甜 tea extract is a TNF-α production inhibitory action, a hyaluronidase activity inhibitory action, a hexosaminidase release inhibitory action, a platelet aggregation inhibitory action, a damage recovery action by UV irradiation, a testosterone 5α-reductase inhibitory action or an androgen receptor. Since it has a binding inhibitory action, through these actions, a TNF-α production inhibitor, a hyaluronidase activity inhibitor, a hexosaminidase release inhibitor, a platelet aggregation inhibitor, an ultraviolet radiation damage recovery agent, a testosterone 5α-reductase inhibitor Or you may use as an active ingredient of an androgen receptor binding inhibitor.

  In addition, the acid hydrolyzate of Hunan 甜 tea extract has TNF-α production inhibitory action, hyaluronidase activity inhibitory action, hexosaminidase release inhibitory action, platelet aggregation inhibitory action, estrogen-like action, type IV collagen production promoting action, ultraviolet light It has a damage recovery action by irradiation, a transglutaminase-1 production promoting action or a testosterone 5α-reductase inhibitory action, and therefore through these actions, a TNF-α production inhibitor, a hyaluronidase activity inhibitor, a hexosaminidase release inhibitor, a platelet It may be used as an active ingredient of an aggregation inhibitor, an estrogen-like agent, a type IV collagen production promoter, a damage recovery agent by UV irradiation, a transglutaminase-1 production promoter or a testosterone 5α-reductase inhibitor.

  The anti-inflammatory agent, anti-aging agent, hair-restoring agent or anti-androgenic agent of the present invention may consist of only Hunan 甜 tea extract and / or acid hydrolyzate of the extract, or Hunan 甜 tea extract. And / or may be formulated from an acid hydrolyzate of the extract.

  Hunan coffee tea extract and / or acid hydrolyzate of the extract is powdery, granular, liquid in accordance with a conventional method using a pharmaceutically acceptable carrier such as dextrin, cyclodextrin or any other auxiliary agent. And can be formulated into any dosage form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring agent and the like can be used. Further, the Hunan coffee tea extract and / or the acid hydrolyzate of the extract can be used by blending with other compositions (for example, skin cosmetics, hair cosmetics, etc. described later), and ointments. It can be used as a solution for external use, a patch and the like.

  In addition, the anti-inflammatory agent of this invention can mix | blend the other natural extract which has an anti-inflammatory action as needed, and can use it as an active ingredient. Moreover, the anti-aging agent of this invention can mix | blend the other natural extract which has an anti-aging effect as needed, and can use it as an active ingredient. Furthermore, the hair restoring agent of the present invention can be used as an active ingredient by blending with other natural extracts having hair restoring action, if necessary. Furthermore, the anti-androgenic agent of the present invention can be used as an active ingredient by blending with other natural extracts having anti-androgenic action as required.

  The anti-inflammatory agent of the present invention is based on the TNF-α production inhibitory action, hyaluronidase activity inhibitory action, hexosaminidase release inhibitory action and platelet aggregation inhibitory action possessed by the Hunan coffee tea extract and / or the acid hydrolyzate of the extract. Through one or more actions selected from the group, contact dermatitis (rash), psoriasis, pemphigus vulgaris, and other various inflammatory skin diseases with rough skin can be prevented, treated or improved. . However, the anti-inflammatory agent of the present invention is one or two selected from the group consisting of TNF-α production inhibitory action, hyaluronidase activity inhibitory action, hexosaminidase release inhibitory action and platelet aggregation inhibitory action in addition to these uses. It can be used for all purposes that are meaningful for exerting more than a kind of action.

  The anti-aging agent of the present invention is a damage recovery effect by UV irradiation of Hunan coffee tea extract, an estrogen-like action of an acid hydrolyzate of Hunan coffee tea extract, a type IV collagen production promoting action, and a damage recovery action by UV irradiation. In addition, skin aging symptoms and the like can be prevented, treated, or ameliorated through one or more actions selected from the group consisting of a transglutaminase-1 production promoting action. However, in addition to these uses, the anti-aging agent of the present invention is one kind selected from the group consisting of an estrogen-like action, a type IV collagen production promoting action, a damage recovery action by ultraviolet irradiation, and a transglutaminase-1 production promoting action. It can be used for all purposes that are meaningful in exerting two or more kinds of actions.

  The hair restorer of the present invention is a male through the testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action possessed by Hunan coffee tea extract, or the testosterone 5α-reductase inhibitory action possessed by the acid hydrolyzate of Hunan coffee tea extract. Can prevent, treat or ameliorate alopecia, hirsutism, seborrhea, acne (acne etc.), benign prostatic hyperplasia, prostate tumor, premature boyhood, etc. Suitable for improvement. However, the hair-restoring agent of the present invention can be used for all purposes other than these uses that are meaningful for exerting testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action.

  The anti-androgenic hormone agent of the present invention has a testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action possessed by Hunan coffee tea extract, or a testosterone 5α-reductase inhibitory action possessed by an acid hydrolyzate of Hunan coffee tea extract. Can prevent, treat or ameliorate diseases involving male hormones, such as androgenetic alopecia, hirsutism, seborrhea, acne (acne etc.), benign prostatic hyperplasia, prostate tumor, premature boyhood, etc. . However, the anti-androgenic hormone agent of the present invention can be used for all purposes that are significant for exerting testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action in addition to these uses.

[Skin cosmetics, hair cosmetics]
Hunan coffee tea extract and / or acid hydrolyzate of the extract has anti-inflammatory action, anti-aging action, hair-growth action or anti-androgen action and is used when applied to skin or hair (scalp) Since it is excellent in feeling or safety, it is suitable for blending into skin cosmetics or hair cosmetics. In this case, the skin cosmetics or hair cosmetics may be directly blended with Hunan coffee tea extract and / or acid hydrolyzate of the extract, or Hunan coffee tea extract and / or acid hydrolyzate of the extract. You may mix | blend the anti-inflammatory agent, anti-aging agent, hair restorer, or antiandrogen agent which were formulated from the degradation product. Hunan coffee tea extract and / or acid hydrolyzate of the extract; anti-inflammatory agent, anti-aging agent, hair restorer or anti-androgen agent formulated from Hunan coffee tea extract and / or acid hydrolyzate of the extract By blending, it is possible to impart an anti-inflammatory action, an anti-aging action, a hair-growth action or an anti-androgen action to skin cosmetics or hair cosmetics.

  The kind of skin cosmetics or hair cosmetics that can be blended with Hunan coffee tea extract and / or acid hydrolyzate of the extract is not particularly limited, and examples of skin cosmetics include ointments, creams, and emulsions. , Lotions, packs, foundations and the like, and examples of hair cosmetics include hair tonics, hair creams, hair liquids, shampoos, pomades, rinses, and the like.

  When blending Hunan coffee tea extract and / or acid hydrolyzate of the extract into skin cosmetics or hair cosmetics, the blending amount can be adjusted as appropriate according to the type of skin cosmetics or hair cosmetics. However, a suitable blending rate is about 0.0001 to 10% by mass in terms of a standard extract, and a particularly suitable blending rate is about 0.001 to 10% in terms of a standard extract. 1% by mass.

  Unless the skin cosmetic or hair cosmetic of the present invention interferes with the anti-inflammatory action, anti-aging action, hair-growth action or anti-androgen action of the Hunan coffee tea extract and / or acid hydrolyzate of the extract, Main agents, auxiliaries or other ingredients used in the manufacture of skin cosmetics or hair cosmetics such as astringents, bactericides / antibacterial agents, ultraviolet absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, Oxidizing / active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like can be used in combination. By using together, it becomes a more general product, and the synergistic action between the above-mentioned components used in combination may bring about a superior use effect than would normally be expected.

  The skin cosmetic of the present invention has high safety, and through one or more actions selected from the group consisting of an anti-inflammatory action, an anti-aging action and an anti-androgen action, contact dermatitis (Rash), psoriasis, pemphigus vulgaris, other inflammatory skin diseases with rough skin, skin aging symptoms, seborrhea, acne (acne etc.), etc. can be prevented, treated or improved. Moreover, the hair cosmetic composition of the present invention has high safety, and can prevent and improve male pattern baldness and the like through a hair growth action and / or an antiandrogenic action.

  The anti-inflammatory agent, anti-aging agent, hair-restoring agent, anti-androgen agent, skin cosmetic or hair cosmetic of the present invention is preferably applied to humans, but has the respective effects. It can also be applied to animals other than humans as long as possible.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1] Manufacture of Hunan coffee tea extract 4000 ml of 50% ethanol by weight was added to 400 g of dried dried leaves of Hunan coffee tea, heated and extracted at 80 ° C for 2 hours with a reflux extractor, and filtered while hot. . The residue was further extracted in the same manner. The obtained extracts were combined, concentrated under reduced pressure, and further dried to obtain 90.4 g of a Konan tea extract (sample 1).

[Production Example 2] Production of acid hydrolyzate of Hunan coffee tea extract 50 ml of a mixed solvent obtained by mixing 95% by weight methanol and 5% by weight hydrochloric acid with 5.0 g of Hunan coffee tea extract obtained in Production Example 1. Ratio (mass basis) = 1: 1.7), and the mixture was reacted for 2 hours under heating and reflux. After completion of the reaction, 1000 mL of water was added, applied to a Diaion HP-20 (Mitsubishi Chemical Corporation) column, and eluted in the order of water and methanol to obtain 2.8 g of an acid hydrolyzate as a methanol fraction (Sample 2). ).

[Test Example 1] TNF-α production inhibitory action test Each sample (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 was tested for TNF-α production inhibitory action as follows.

After culturing mouse macrophage cells (RAW264.7) using Dulbecco's MEM medium containing 10% FBS, the cells were collected with a cell scraper. The collected cells were diluted with Dulbecco's MEM medium containing 10% FBS to a cell density of 1.0 × 10 ⁶ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured for 4 hours.

  After completion of the culture, the medium was removed, and 100 μL of a sample solution (samples 1 and 2) dissolved using Dulbecco's MEM medium containing 10% FBS containing 2% DMSO at a final concentration was added to each well at a final concentration of 1 μg / mL. 100 μL of lipopolysaccharide (LPS, E. coli 0111; B4, manufactured by DIFCO) dissolved in Dulbecco MEM containing 10% FBS was added and cultured for 24 hours. After completion of the culture, the amount of TNF-α in the culture supernatant of each well was measured using a sandwich ELISA method, and the TNF-α production inhibition rate (%) was calculated from the measurement result according to the following formula.

TNF-α production inhibition rate (%) = {(B−A) / B} × 100
In the formula, A represents “a TNF-α amount when a sample solution is added”, and B represents a “a TNF-α amount when a sample solution is not added”.

The concentration of the sample solution is decreased stepwise to calculate the TNF-α production suppression rate at each concentration, and the sample concentration IC ₅₀ (μg / g) that inhibits TNF-α production by 50% from the result is calculated. mL).
The results are shown in Table 1.

[Table 1]
Sample IC ₅₀ (μg / mL)
Sample 1 200
Sample 2 50

  As shown in Table 1, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have an excellent TNF-α production inhibitory action. In addition, it was confirmed that the degree of TNF-α production inhibitory action can be adjusted by the concentration of Hunan 甜 tea extract and / or its acid hydrolyzate.

Test Example 2 Hyaluronidase Activity Inhibitory Action Test Each sample (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 was tested for hyaluronidase activity inhibitory action as follows.

  A hyaluronidase solution (Type IV-S (from bovine testis), SIGMA) was added to 0.2 mL (sample concentration: 400 μg / mL) of 0.1 mol / L acetate buffer (pH 3.5) in which the samples (Samples 1 and 2) were dissolved. (0.1N, 400 NFU / mL) was added and reacted at 37 ° C. for 20 minutes. Furthermore, 0.2 mL of 2.5 mmol / L calcium chloride was added as an activator and reacted at 37 ° C. for 20 minutes. To this, 0.5 mL of 0.4 mg / mL potassium hyaluronate solution (from Rooster Comb) was added and reacted at 37 ° C. for 40 minutes.

  Thereafter, 0.2 mL of 0.4 mol / L sodium hydroxide was added to stop the reaction, and after cooling, 0.2 mL of boric acid solution was added to each reaction solution and boiled for 3 minutes. After cooling with ice, 6 mL of p-DABA reagent was added and reacted at 37 ° C. for 20 minutes. Thereafter, the absorbance at a wavelength of 585 nm was measured. A blank test was performed and corrected in the same manner. From the obtained measurement results, the hyaluronidase activity inhibition rate (%) was calculated by the following formula.

Inhibition rate (%) = {1− (St−Sb) / (Ct−Cb)} × 100
In the formula, St represents “absorbance of the sample solution at a wavelength of 585 nm”, Sb represents “absorbance of the sample solution at a wavelength of 585 nm”, Ct represents “absorbance of the control solution at a wavelength of 585 nm”, and Cb represents “control”. The absorbance of the solution blank at a wavelength of 585 nm is expressed.
The results are shown in Table 2.

[Table 2]
Sample Hyaluronidase activity inhibition rate (%)
Sample 1 25.8
Sample 2 95.3

  From the results shown in Table 2, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have an excellent hyaluronidase activity inhibitory action.

[Test Example 3] Hexosaminidase release inhibitory action test Each sample (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 was tested for hexosaminidase release inhibitory action as follows.

Rat basophil leukemia cells (RBL-2H3) were cultured in S-MEM medium supplemented with 15% FBS, and then cells were collected by trypsin treatment. The collected cells are diluted to a cell density of 4.0 × 10 ⁵ cells / mL, DNP-specific IgE is added to a final concentration of 0.5 μg / mL, and then seeded at 100 μL per well in a 96-well plate. And cultured overnight.

After completion of the culture, the medium was removed and washed twice with 500 μL of Siligalian buffer. Next, 30 μL of the same buffer solution and 10 μL of the sample solution prepared with the same buffer solution were added, and the mixture was allowed to stand at 37 ° C. for 10 minutes. Thereafter, 10 μL of a 100 ng / mL DNP-BSA solution was added, and the mixture was allowed to stand at 37 ° C. for 15 minutes to release hexosaminidase. Thereafter, the release was stopped by allowing the 96-well plate to stand on ice. 10 μL of cell supernatant in each well and 10 μL of 1 mmol / L p-NAG (p-nitrophenyl-N-acetyl-β-D-glucoside) solution were added to a new 96-well plate and incubated at 37 ° C. for 1 hour. Reacted. After completion of the reaction, 250 μL of 0.1 mol / L Na ₂ CO ₃ / NaHCO ₃ was added to each well, and the absorbance at a wavelength of 415 nm was measured. As a blank test, the absorbance at a wavelength of 415 nm of a mixed solution of 10 μL of cell supernatant and 250 μL of 0.1 mol / L Na ₂ CO ₃ / NaHCO ₃ was measured and corrected. From the obtained measurement results, the hexosaminidase release inhibition rate (%) was calculated by the following formula.

Inhibition rate of hexosaminidase release (%) = {1− (B−C) / A} × 100
In the formula, A represents “absorbance at a wavelength of 415 nm without addition of a sample”, B represents “absorbance at a wavelength of 415 nm with addition of a sample”, and C represents “at a wavelength of 415 nm with addition of a sample and no p-NAG added”. Absorbance ".

The concentration of the sample solution is decreased stepwise to calculate the hexosaminidase release inhibition rate, and the sample concentration IC ₅₀ (μg / mL) that inhibits hexosaminidase release by 50% from the result is calculated. )
The results are shown in Table 3.

[Table 3]
Sample IC ₅₀ (μg / ml)
Sample 1 406
Sample 2 40.9

  From the results shown in Table 3, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have an excellent hexosaminidase release inhibitory action. Moreover, it was confirmed that the degree of hexosaminidase release inhibitory action can be adjusted by the concentration of Hunan 甜 tea extract and / or its acid hydrolyzate.

[Test Example 4] Platelet aggregation inhibitory action test Each sample (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 was tested for platelet aggregation inhibitory action as follows.

(1) Preparation of platelet suspension 1/10 amount of 77 mmol / L EDTA (pH 7.4) was added to the collected rabbit blood, centrifuged (180 × g, 10 minutes, room temperature), and platelet suspension (P RP) was obtained. Further centrifugation (810 × g, 10 minutes, 4 ° C.) was performed, and the supernatant was removed to obtain platelets. This was washed with a platelet washing solution (0.15 mol / L sodium chloride, 0.15 mol / L Tris-HCl buffer (pH 7.4), and 77 mmol / L EDTA solution (pH 7.4) in 90: 8: 2. The resulting platelets were centrifuged in the same manner as above, and the resulting platelets were suspended in platelets (10 mmol / L containing 145 mmol / L sodium chloride, 5 mmol / L potassium chloride and 5.5 mmol / L glucose). The platelet count was adjusted (3.0 × 10 ⁵ cells / μL) by suspending in HEPES buffer (pH 7.4) to obtain a washed platelet suspension.

(2) Platelet aggregation inhibitory action test 1 μL of 200 mmol / L calcium chloride solution was added to 222 μL of the obtained washed platelet suspension and reacted at 37 ° C. for 1 minute. 2 μL of the sample solution was added thereto, and the mixture was further reacted for 2 minutes. After a stir bar was added and stirred for 1 minute, 25 μL of the collagen solution was added, and the platelet aggregation rate for 10 minutes was measured at a temperature of 37 ° C. As a control, the platelet aggregation rate was measured in the same manner except that no sample solution was added. From the measurement results obtained, the platelet aggregation inhibition rate (%) was calculated by the following formula.

Platelet aggregation inhibition rate (%) = (A−B) / A × 100
In the formula, A represents “control platelet aggregation rate”, and B represents “platelet aggregation rate at the time of sample addition”.
The results are shown in Table 4.

[Table 4]
Specimen sample concentration ([mu] g / mL) platelet aggregation inhibition rate (%)
Sample 1 25 69.6
Sample 2 400 19.6

  From the results shown in Table 4, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have a platelet aggregation inhibitory action. In particular, it was confirmed that Hunan 甜 tea extract (Sample 1) has an excellent platelet aggregation inhibition rate.

[Test Example 5] Estrogen-like action test Sample 2 obtained in Production Example 2 was tested for estrogen-like action as follows.

Human breast cancer-derived cells (MCF-7) were cultured in a MEM medium containing 10% FBS, 1% NEAA and 1 mmoL / L sodium pyruvate, and then cells were collected by trypsin treatment. The collected cells were treated with activated carbon-treated 10% FBS, 1% NEAA and 1 mmol / L sodium pyruvate, and the phenol red-free MEM (T-MEM) medium was used at 3.0 × 10 ⁴ cells / After dilution to a cell density of mL, the cells were seeded at 450 μL per well in a 48-well plate and cultured to establish the cells.

  Six hours later (day 0), 50 μL of a sample solution (sample concentration: 12.5 μg / mL) prepared to 10 times the final concentration with T-MEM was added to each well, and the culture was continued. On the third day, the medium was removed, 0.5 mL of the sample solution prepared to the final concentration with T-MEM was added to each well, and the culture was further continued.

Estrogen-like effects were measured using the MTT assay. After completion of the culture, the medium was removed, and 200 μL of MTT dissolved in MEM containing 1% NEAA and 1 mmol / L sodium pyruvate at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 200 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. As a positive control, 1 × 10 ⁻⁹ M estradiol was used. From the measurement results obtained, the estrogen-like action rate (%) was calculated by the following formula.

Estrogen-like action rate (%) = A / B × 100
In the formula, A represents “absorbance when a sample is added”, and B represents “absorbance when no sample is added”.

  As a result of calculation by the above formula, the estrogen-like action rate of the acid hydrolyzate (sample 2) of Hunan 甜 tea extract is 214.3 ± 2.0%, and the acid hydrolyzate (sample 2) of Hunan 甜 tea extract ) Was confirmed to have an excellent estrogenic effect.

[Test Example 6] Type IV collagen production promoting action test Sample 2 obtained in Production Example 2 was tested for type IV collagen production promoting action as follows.

Human normal fibroblasts (NB1RGB) were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with Dulbecco's MEM medium to a cell density of 1.6 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well microplate, and cultured overnight.

  After completion of the culture, the medium was removed, 150 μL of a sample solution (sample concentration: 100 μg / mL) dissolved in 0.25% FBS-containing Dulbecco's MEM medium was added to each well and cultured for 3 days. After culture, the amount of type IV collagen in the medium of each well was measured by ELISA. From the obtained measurement results, the type IV collagen production promotion rate (%) was calculated by the following formula.

Type IV collagen production promotion rate (%) = A / B × 100
In the formula, A represents “amount of type IV collagen when a sample is added”, and B represents “amount of type IV collagen when no sample is added”.

  As a result of calculation by the above formula, the rate of type IV collagen production promotion of the acid hydrolyzate (sample 2) of Hunan coffee tea extract is 209.3 ± 19.2%, and the acid hydrolyzate of Hunan coffee tea extract ( Sample 2) was confirmed to have an excellent type IV collagen production promoting effect.

[Test Example 7] Damage recovery action test by UV irradiation The samples (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 were tested for damage recovery action by UV irradiation as follows.

Human normal skin fibroblasts (NB1RGB) were cultured using α-MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with α-MEM medium to a cell density of 2.0 × 10 ⁵ cells / mL, and then seeded at 200 μL per well in a 48-well plate. After culturing for 24 hours, the medium was replaced with 100 μL of PBS (−) and irradiated with 1.0 J / cm ² of UV-B. Immediately after the irradiation, PBS (-) was taken out, 400 µL of a sample solution (sample concentration; 100 µg / mL) dissolved in 10% FBS-containing α-MEM was added to each well, and cultured for 24 hours.

  The damage recovery effect by ultraviolet (UV-B) irradiation was measured using an MTT assay. After completion of the culture, the medium was removed, and 200 μL of MTT dissolved at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 200 μL of 2-propanol, and after extraction, absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. Similarly, after seeding the cells, the cells not irradiated with UV-B and the cells irradiated with UV-B but not added with the sample solution were also measured in the same manner, and were set as a non-irradiated group and an irradiated group, respectively. From the obtained measurement results, the damage recovery rate (%) by ultraviolet (UV-B) irradiation was calculated by the following formula.

Damage recovery rate (%) = {(Nt−C) − (Nt−Sa)} / (Nt−C) × 100
In the formula, Nt represents “absorbance in cells not irradiated with UV-B”, C represents “absorbance in cells irradiated with UV-B but not added with a sample solution”, and Sa represents “UV Absorbance in “cells irradiated with B and added with sample solution”.
The results are shown in Table 5.

[Table 5]
Sample Damage recovery rate by UV irradiation (%)
Sample 1 7.1 ± 1.9
Sample 2 53.4 ± 1.8

  As shown in Table 5, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have a damage recovery effect by ultraviolet irradiation. In particular, it was confirmed that the acid hydrolyzate (sample 2) of Hunan 甜 tea extract has an excellent damage recovery action by ultraviolet rays.

[Test Example 8] Transglutaminase-1 production promoting action test Sample 2 obtained in Production Example 2 was tested for transglutaminase-1 production promoting action as follows.

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocyte medium (KGM), and then cells were collected by trypsin treatment. The collected cells were diluted with KGM to a cell density of 1.0 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured for 2 days.

  After completion of the culture, 100 μL of a sample solution dissolved in KGM (sample concentration: 25 μg / mL) was added to each well and cultured for 24 hours. After completion of the culture, the medium was removed, the cells were fixed on a plate, and the amount of transglutaminase-1 expressed on the cell surface was measured by ELISA using a monoclonal anti-human transglutaminase-1 antibody. From the obtained measurement results, the transglutaminase-1 production promotion rate (%) was calculated by the following formula.

Transglutaminase-1 production promotion rate (%) = A / B × 100
In the formula, A represents “absorbance at a wavelength of 405 nm when a sample is added”, and B represents “absorbance at a wavelength of 405 nm when no sample is added”.

  As a result of calculation by the above formula, the transglutaminase-1 production promotion rate of the acid hydrolyzate of Hunan coffee tea extract (sample 2) is 115.5 ± 1.9%, and the acid hydrolyzate of Hunan coffee tea extract (Sample 2) was confirmed to have an excellent transglutaminase-1 production promoting action.

[Test Example 9] Testosterone 5α-reductase inhibitory action test Each sample (Samples 1 and 2) obtained in Production Example 1 and Production Example 2 was tested for testosterone 5α-reductase inhibitory action as follows.

  In a V-bottom test tube with a lid, 20 μL of testosterone (manufactured by Wako Pure Chemical Industries, Ltd.) dissolved in 1 mL of propylene glycol and 5 mmol / mL Tris-HCl buffer (pH 7) containing 1 mg / mL NADPH. .13) 825 μL was mixed.

  Further, 80 μL of an ethanol aqueous solution of each sample (Samples 1 and 2) and 75 μL of S-9 (rat liver homogenate, manufactured by Oriental Yeast Co., Ltd.) were added and mixed, and incubated at 37 ° C. for 30 minutes. Thereafter, 1 mL of methylene chloride was added to stop the reaction. This was centrifuged (1600 × g, 10 minutes), the methylene chloride layer was separated, and the separated methylene chloride layer was subjected to gas chromatography analysis under the following conditions to obtain 3α-androstanediol, 5α. -The concentration of dihydrotestosterone (5α-DHT) and testosterone (μg / mL) was quantified. As a control, the same amount (80 μL) of the sample solvent was used instead of the sample solution, and the same treatment was performed for gas chromatography analysis.

[Gas chromatography conditions]
Equipment used: Shimadzu GC-7A (manufactured by Shimadzu Corporation)
Column: DB-1701 (inner diameter: 0.53 mm, length: 30 m, film thickness: 1.0 μm, manufactured by J & W Scientific)
Column temperature: 240 ° C
Inlet temperature: 300 ° C
Detector: FID
Sample injection volume: 1 μL
Split ratio: 1: 2
Carrier gas: Nitrogen gas Carrier gas flow rate: 3 mL / min

Quantification of the concentrations of 3α-androstanediol, 5α-DHT and testosterone was performed by the following method.
Standard products of 3α-androstanediol, 5α-DHT and testosterone were dissolved in methylene chloride, and the solution was subjected to gas chromatography analysis. From the concentration (μg / mL) and peak area of these compounds, peak area and compound Correspondence with the concentration of was previously determined. Then, the concentration per peak area of 3α-androstanediol, 5α-DHT and testosterone after the reaction of testosterone and S-9 is calculated using the following formula (1). Based on.

A = B × C / D (1)
In the formula, A represents “3α-androstanediol, 5α-DHT or testosterone concentration (μg / mL)”, B represents “3α-androstanediol, 5α-DHT or testosterone peak area”, and C Represents “concentration of standard product (μg / mL)”, and D represents “peak area of standard product”.

  Using the compound concentration calculated based on the formula (1), based on the following formula (2), the conversion rate (the concentrations of 3α-androstanediol and 5α-DHT produced by reducing testosterone by testosterone 5α-reductase) And the ratio of the initial concentration of testosterone).

Conversion rate = (E + F) / (E + F + G) (2)
In the formula, E represents “3α-androstanediol concentration (μg / mL)”, F represents “5α-DHT concentration (μg / mL)”, and G represents “testosterone concentration (μg / mL)”. ".

Using the conversion rate calculated based on the formula (2), the testosterone 5α-reductase inhibition rate (%) was calculated based on the following formula (3).
Inhibition rate (%) = (1-H / I) × 100 (3)
In the formula, H represents “conversion rate at the time of sample addition”, and I represents “control conversion rate”.

The inhibition rate was measured by gradually reducing the sample concentration, and the sample concentration IC ₅₀ (μg / mL) at which the testosterone 5α-reductase inhibition rate was 50% was determined by interpolation.
The results are shown in Table 6.

[Table 6]
Sample IC ₅₀ (μg / mL)
Sample 1 1123
Sample 2 201

  As shown in Table 6, it was confirmed that Hunan 甜 tea extract (sample 1) and its acid hydrolyzate (sample 2) have an excellent testosterone 5α-reductase inhibitory action. Moreover, it was confirmed that the degree of testosterone 5α-reductase inhibitory action can be adjusted by the concentration of Hunan 甜 tea extract and / or its acid hydrolyzate.

Test Example 10 Androgen Receptor Binding Inhibitory Action Test Sample 1 obtained in Production Example 1 was tested for androgen receptor binding inhibitory action as follows.

Androgen-dependent mouse breast cancer cells (SC-3 cells) cloned from mouse spontaneous breast cancer (Shionogi cancer; SC115) were cultured in MEM medium containing 2% DCC-FBS and 10 ⁻⁸ mol / L testosterone (hereinafter, After culturing using MEM-2, the cells were collected by trypsin treatment. The collected cells are diluted with MEM-2 medium so as to have a cell density of 1.0 × 10 ⁵ cells / μL, seeded at 100 μL per well in a 96-well plate, 37 ° C., 5% CO ₂ -95%. The cells were cultured under air conditions. After 24 hours, the medium was replaced with the sample (sample 1) and 0.5% BSA-containing HamF12 + MEM medium (hereinafter referred to as HMB medium) supplemented with 1.0 × 10 ⁻⁹ mol / L DHT, and cultured for 48 hours. did. Thereafter, the medium was replaced with MEM-2 medium containing 0.97 mmol / L of MTT, and after culturing for 2 hours, the medium was replaced with isopropanol, and blue formazan produced in the cells was extracted. For the isopropanol containing the eluted blue formazan, the absorbance at 570 nm where the absorption maximum of blue formazan is present was measured.

  In order to correct the influence of adherent cells, the absorbance at 650 nm was also measured at the same time, and the difference between the two absorbances was taken as a value proportional to the amount of blue formazan produced (the absorbance in the formula for calculating the binding inhibition rate is the corrected absorbance below). Is). In parallel with the above, in order to examine the effect of the sample alone on the SC-3 cells, the same culture and measurement were performed by adding only the sample without adding DHT to the HMB medium. Further, as a control, the same measurement was performed when culturing in an HMB medium without addition of a sample and DHT, and when culturing in an HMB medium without addition of a sample and only DHT. From the measurement results, the androgen receptor binding inhibition rate (%) was calculated by the following formula.

Androgen receptor binding inhibition rate (%) = {1− (A−B) / (C−D)} × 100
In the formula, A represents “absorbance when DHT is added / sample added”, B represents “absorbance when DHT is not added / sample is added”, and C is “absorbance when DHT is added / sample is not added”. D represents “absorbance when DHT is not added / sample is not added”.

The concentration of the sample solution is decreased stepwise to measure the inhibition rate, and the inhibition rate of androgen binding (%) at each concentration is determined. From the result, androgen binding to the receptor is determined by interpolation. The sample concentration IC ₅₀ (μg / mL) that inhibits% was determined.

The IC ₅₀ of the Hunan coffee extract (Sample 1) was 60.0 μg / mL, and it was confirmed that the Hunan coffee tea extract (Sample 1) has an excellent androgen receptor binding inhibitory action. In addition, it was confirmed that the degree of the androgen receptor binding inhibitory action can be adjusted by the concentration of Hunan 甜 tea extract.

[Formulation Example 1]
An emulsion having the following composition was produced by a conventional method.
Hunan 甜 tea extract (Production Example 1) 0.1g
Jojoba oil 4.0g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Polyoxyethylene cetyl ether (20E.O.) 2.5g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0g
Twilight extract 0.1g
0.1g dipotassium glycyrrhizinate
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Chamomile extract 0.1g
1,3-butylene glycol 3.0 g
Methyl paraoxybenzoate 0.15g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A lotion having the following composition was produced by a conventional method.
Hunan 甜 tea acid hydrolyzate (Production Example 2) 0.1g
Glycerin 3.0g
1,3-butylene glycol 3.0 g
Oleic acid polyoxyethylene sorbitan (20E.O.) 0.5g
Methyl paraoxybenzoate 0.15g
Citric acid 0.1g
Sodium citrate 0.1g
Oil soluble licorice extract 0.1g
Seaweed extract 0.1g
Xylobiose Mixture 0.5g
Kujin extract 0.1g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A cream having the following composition was produced by a conventional method.
Hunan 甜 tea extract (Production Example 1) 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
1,3-butylene glycol 6.0 g
Yeast extract 0.1g
Perilla extract 0.1g
Linden extract 0.1g
Jiuyu Extract 0.1g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A pack having the following composition was produced by a conventional method.
Hunan 甜 tea acid hydrolyzate (Production Example 2) 0.2g
Polyvinyl alcohol 15.0g
Polyethylene glycol 3.0g
Propylene glycol 7.0g
Ethanol 10.0g
Sage extract 0.1g
Toki extract 0.1g
Carrot extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 5]
A hair nourishing hair tonic having the following composition was produced by a conventional method.
Hunan coffee extract (Production Example 1) 0.2g
0.1 g of pyridoxine hydrochloride
Resorcin 0.01g
D-pantothenyl alcohol 0.1g
0.1g dipotassium glycyrrhizinate
L-Menthol 0.05g
1,3-butylene glycol 4.0 g
Carrot extract 0.5g
Ethanol 25.0g
Fragrance 0.01g
Purified water remainder (total amount is 100 g)

[Composition Example 6]
A shampoo (cream shampoo) having the following composition was produced by a conventional method.
Hunan 甜 tea acid hydrolyzate (Production Example 2) 0.2g
Sodium polyoxyethylene alkyl ether sulfate 30.0g
Polyoxyethylene alkyl ether ammonium sulfate 20.0g
Coconut oil fatty acid amidopropyl betaine 6.0g
Coconut oil fatty acid modiethanolamide 4.0g
2.0g ethylene distearate
Preservative (Methyl paraoxybenzoate) 0.15g
Mukuroji extract 0.2g
Twilight extract 0.5g
Oat extract 0.3g
Rosemary extract 0.5g
1,3-butylene glycol 3.0 g
Fragrance 0.01g
Purified water remainder (total amount is 100 g)

[Formulation Example 7]
A rinse having the following composition was produced by a conventional method.
Hunan coffee extract (Production Example 1) 0.2g
Stearyltrimethylammonium chloride 1.5g
Polyoxyethylene cetyl ether 1.0g
Cetyl alcohol 2.0g
Octyldodecanol 1.0g
Cationized cellulose 0.5g
Propylene glycol 5.0g
Mukuroji extract 0.2g
Twilight extract 0.5g
Oat extract 0.3g
Rosemary extract 0.5g
Fragrance 3.0g
Purified water remainder (total amount is 100 g)

  The anti-inflammatory agent of the present invention is used for the prevention / improvement of various inflammatory diseases, and the anti-aging agent of the present invention is used for the prevention / improvement of skin aging such as skin hypersensitivity, decreased elasticity, and reduction of moisture. The hair-restoring agent or anti-androgen agent of the invention greatly contributes to the prevention and improvement of androgenetic alopecia, hirsutism, seborrhea, acne (acne etc.), benign prostatic hyperplasia, prostate tumor, premature boyhood, etc. Can do.

Claims (4)

  A hair restorer comprising, as an active ingredient, an extract from Linancarpus litseifolius and / or an acid hydrolyzate of the extract. The extract has testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action,
The hair restorer according to claim 1, wherein the acid hydrolyzate has a testosterone 5α-reductase inhibitory action.   An anti-androgen agent characterized by containing an extract from Lithocarpus litseifolius and / or an acid hydrolyzate of the extract as an active ingredient. The extract has testosterone 5α-reductase inhibitory action and / or androgen receptor binding inhibitory action,
The anti-androgenic agent according to claim 3, wherein the acid hydrolyzate has a testosterone 5α-reductase inhibitory action.