JP5663841B2 - 骨細胞への分化誘導培地及び方法 - Google Patents

骨細胞への分化誘導培地及び方法 Download PDF

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Publication number
JP5663841B2
JP5663841B2 JP2009038998A JP2009038998A JP5663841B2 JP 5663841 B2 JP5663841 B2 JP 5663841B2 JP 2009038998 A JP2009038998 A JP 2009038998A JP 2009038998 A JP2009038998 A JP 2009038998A JP 5663841 B2 JP5663841 B2 JP 5663841B2
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Prior art keywords
medium
differentiation
vitamin
cells
stem cells
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Expired - Fee Related
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JP2009038998A
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English (en)
Japanese (ja)
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JP2010193723A (ja
JP2010193723A5 (https=
Inventor
佳之 堀田
佳之 堀田
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Fujirebio Inc
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Fujirebio Inc
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Priority to JP2009038998A priority Critical patent/JP5663841B2/ja
Priority to EP10743811.1A priority patent/EP2399987B1/en
Priority to US13/202,713 priority patent/US9315777B2/en
Priority to CA2753303A priority patent/CA2753303A1/en
Priority to PCT/JP2010/052455 priority patent/WO2010095685A1/ja
Publication of JP2010193723A publication Critical patent/JP2010193723A/ja
Publication of JP2010193723A5 publication Critical patent/JP2010193723A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2009038998A 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法 Expired - Fee Related JP5663841B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2009038998A JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法
EP10743811.1A EP2399987B1 (en) 2009-02-23 2010-02-18 Culture medium and method to induce differentiation into bone cells
US13/202,713 US9315777B2 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells
CA2753303A CA2753303A1 (en) 2009-02-23 2010-02-18 Culture medium and method for inducing differentiation into bone cells
PCT/JP2010/052455 WO2010095685A1 (ja) 2009-02-23 2010-02-18 骨細胞への分化誘導培地及び方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2009038998A JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法

Publications (3)

Publication Number Publication Date
JP2010193723A JP2010193723A (ja) 2010-09-09
JP2010193723A5 JP2010193723A5 (https=) 2012-04-05
JP5663841B2 true JP5663841B2 (ja) 2015-02-04

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JP2009038998A Expired - Fee Related JP5663841B2 (ja) 2009-02-23 2009-02-23 骨細胞への分化誘導培地及び方法

Country Status (5)

Country Link
US (1) US9315777B2 (https=)
EP (1) EP2399987B1 (https=)
JP (1) JP5663841B2 (https=)
CA (1) CA2753303A1 (https=)
WO (1) WO2010095685A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2015003233A (es) * 2012-09-13 2015-11-16 Univ Leland Stanford Junior Estimulacion del desarrollo de policulo del ovario y maduracion del oocito.
CN113025566A (zh) * 2020-12-30 2021-06-25 无锡市第九人民医院 一种内皮细胞成骨诱导分化培养基及制备方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69734395T2 (de) * 1996-12-06 2006-07-27 Osiris Therapeutics, Inc. Verbesserte chondrogene differenzierung von menschlichen mesenchym-stammzellen
US6153582A (en) * 1998-11-05 2000-11-28 Bausch & Lomb Surgical, Inc. Defined serumfree medical solution for ophthalmology
CA2488425A1 (en) 2002-06-07 2003-12-18 Es Cell International Pte Ltd Methods of regulating differentiation in stem cells
AU2004261899A1 (en) * 2003-08-01 2005-02-10 Norimasa Nakamura Scaffold-free self-organized 3D synthetic tissue
JP2006055106A (ja) 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
US9074176B2 (en) * 2006-01-13 2015-07-07 Two Cells Co., Ltd. Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof
LV13634B (en) 2007-05-02 2007-12-20 Jp Biotechnology Sia Production of ethanol and feedstuffs
KR101574989B1 (ko) * 2007-12-28 2015-12-21 후지레비오 가부시키가이샤 포유 동물 체세포용 배지 및 이를 위한 첨가제
JP5515319B2 (ja) * 2009-02-23 2014-06-11 富士レビオ株式会社 脂肪細胞への分化誘導培地及び方法

Also Published As

Publication number Publication date
EP2399987A1 (en) 2011-12-28
JP2010193723A (ja) 2010-09-09
EP2399987A4 (en) 2013-12-25
US20110306132A1 (en) 2011-12-15
WO2010095685A1 (ja) 2010-08-26
CA2753303A1 (en) 2010-08-26
EP2399987B1 (en) 2015-11-04
US9315777B2 (en) 2016-04-19

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