JP5617075B2 - 酵素 - Google Patents

酵素 Download PDF

Info

Publication number
JP5617075B2
JP5617075B2 JP2010548164A JP2010548164A JP5617075B2 JP 5617075 B2 JP5617075 B2 JP 5617075B2 JP 2010548164 A JP2010548164 A JP 2010548164A JP 2010548164 A JP2010548164 A JP 2010548164A JP 5617075 B2 JP5617075 B2 JP 5617075B2
Authority
JP
Japan
Prior art keywords
nucleic acid
dna polymerase
seq
polypeptide
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010548164A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011512812A5 (enExample
JP2011512812A (ja
Inventor
ロイ クラーク，ダンカン
ロイ クラーク，ダンカン
モラント，ニコラス
Original Assignee
ジェネシス バイオテック リミティド
ジェネシス バイオテック リミティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ジェネシス バイオテック リミティド, ジェネシス バイオテック リミティド filed Critical ジェネシス バイオテック リミティド
Publication of JP2011512812A publication Critical patent/JP2011512812A/ja
Publication of JP2011512812A5 publication Critical patent/JP2011512812A5/ja
Application granted granted Critical
Publication of JP5617075B2 publication Critical patent/JP5617075B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2010548164A 2008-02-28 2009-02-13 酵素 Active JP5617075B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0803628.7 2008-02-28
GB0803628A GB0803628D0 (en) 2008-02-28 2008-02-28 Enzyme
PCT/GB2009/000411 WO2009106795A1 (en) 2008-02-28 2009-02-13 Enzyme

Publications (3)

Publication Number Publication Date
JP2011512812A JP2011512812A (ja) 2011-04-28
JP2011512812A5 JP2011512812A5 (enExample) 2012-02-02
JP5617075B2 true JP5617075B2 (ja) 2014-11-05

Family

ID=39284692

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010548164A Active JP5617075B2 (ja) 2008-02-28 2009-02-13 酵素

Country Status (5)

Country Link
US (1) US8986968B2 (enExample)
EP (1) EP2247607B1 (enExample)
JP (1) JP5617075B2 (enExample)
GB (1) GB0803628D0 (enExample)
WO (1) WO2009106795A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2240576B1 (en) * 2008-01-11 2012-10-03 Genesys Ltd Cren7 chimeric protein
GB0804721D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804722D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
CN113637085B (zh) * 2020-05-11 2024-01-30 苏州先达基因科技有限公司 融合dna聚合酶突变体及其在等温扩增中的应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5047342A (en) * 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
US5270179A (en) * 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
EP0550687B1 (en) 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
US5948663A (en) * 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
JP3112148B2 (ja) 1995-05-31 2000-11-27 東洋紡績株式会社 核酸の増幅方法およびその試薬
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
ATE406456T1 (de) 2001-11-28 2008-09-15 Bio Rad Laboratories Verfahren zur verwendung verbesserter polymerasen
US7052877B2 (en) * 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
US7666645B2 (en) 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
KR20070032737A (ko) * 2004-06-04 2007-03-22 다카라 바이오 가부시키가이샤 Ｄｎａ 폴리메라아제 활성을 갖는 폴리펩티드
WO2006030455A1 (en) 2004-09-17 2006-03-23 Prokaria Ehf. Dna polymerases having strand displacement activity
EP1836319B1 (en) 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptides having nucleic acid binding activity and methods for nucleic acid amplification
US9040276B2 (en) * 2005-07-15 2015-05-26 Agilent Technologies, Inc. DNA binding protein-polymerase chimeras
KR100777227B1 (ko) 2005-10-08 2007-11-28 한국해양연구원 고호열성 ｄｎａ 중합효소 및 이의 제조방법
WO2007127893A2 (en) 2006-04-28 2007-11-08 Ge Healthcare Bio-Sciences Corp. Thermostable dna polymerase from thermotoga naphthophila and thermotoga petrophellia
EP2240576B1 (en) * 2008-01-11 2012-10-03 Genesys Ltd Cren7 chimeric protein
GB0804721D0 (en) 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804722D0 (en) 2008-03-14 2008-04-16 Genesys Ltd Enzyme

Also Published As

Publication number Publication date
GB0803628D0 (en) 2008-04-02
US8986968B2 (en) 2015-03-24
EP2247607B1 (en) 2013-10-02
JP2011512812A (ja) 2011-04-28
WO2009106795A1 (en) 2009-09-03
EP2247607A1 (en) 2010-11-10
US20110008848A1 (en) 2011-01-13

Similar Documents

Publication Publication Date Title
KR100882711B1 (ko) 사이크로박터 스피시스 ｈｊ147 균주 유래의 우라실-ｄｎａ글리코실라제 및 이의 용도
EP2981609B1 (en) Novel dna-polymerases
JP5617075B2 (ja) 酵素
EP2252687B1 (en) A thermostable dna polymerase from palaeococcus helgesonii
Kim et al. Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp. NA1.
JP5394928B2 (ja) サーマス・エガートソニイ（ｔｈｅｒｍｕｓｅｇｇｅｒｔｓｓｏｎｉｉ）ｄｎａポリメラーゼ
JP6720632B2 (ja) 融合タンパク質
WO2006030455A1 (en) Dna polymerases having strand displacement activity
JP7014256B2 (ja) 核酸増幅試薬
KR100777227B1 (ko) 고호열성 ｄｎａ 중합효소 및 이의 제조방법
EP2252692B1 (en) A thermostable dna polymerase from palaeococcus ferrophilus
US20100297706A1 (en) Mutant dna polymerases and their genes from thermococcus
US20110020896A1 (en) Mutant dna polymerases and their genes
US20080311626A1 (en) Dna Polymerases Having Strand Displacement Activity
EP1075525B1 (en) Dna polymerase from pyrobaculum islandicum
KR100969477B1 (ko) 써모코커스 구아이마센시스 균주 유래 내열성 ｄｎａ중합효소 및 이를 이용한 중합효소 연쇄반응 방법
WO2024262588A1 (ja) 核酸増幅における増幅バイアスの低減方法
KR101296882B1 (ko) 써모코커스 와이오타푸엔시스 균주 유래의 ｄｎａ 중합효소 변이체들 및 이의 이용
JP2000308494A (ja) 超耐熱性ｄｎａリガーゼ
JP2001299348A (ja) Ｄｎａポリメラーゼ活性と３’−５’エキソヌクレアーゼ活性を有する耐熱性ヘテロダイマー酵素およびその製法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111206

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20111206

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130514

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20130717

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130724

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131114

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20131114

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140218

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140519

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20140520

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20140612

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140708

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20140807

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140807

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140822

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140822

R150 Certificate of patent or registration of utility model

Ref document number: 5617075

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250