JP5576080B2 - β-glucan-containing composition - Google Patents

Description

  The present invention relates to a composition containing β-glucan, and more particularly to a β-glucan-containing composition used for the production of cosmetics.

  Conventionally, water-soluble polymers have been widely used for the purpose of increasing the viscosity of cosmetics, improving stability, and adjusting the feeling of use. Examples of water-soluble polymers include natural polymers such as guar gum, carrageenan, casein, and xanthan gum, semi-synthetic polymers such as carboxymethyl starch, propylene glycol alginate, methylcellulose, hydroxypropylcellulose, and carboxymethylcellulose, polyacrylic acid, carboxy Synthetic polymers such as vinyl polymers are appropriately selected and used depending on the purpose.

  Usually, in order to improve the stability of cosmetics using the protective colloid properties of water-soluble polymers, it is necessary to add a large amount of water-soluble polymer, but on the other hand, a large amount of water-soluble polymer was added. In this case, a slimy feeling peculiar to a water-soluble polymer is produced, and an unfavorable feeling of use arises. Β-glucan is an example of a compound that does not have such a problem, has a high protective colloid property, and does not have a slimy feeling peculiar to a water-soluble polymer even when blended in a large amount, and has a good usability. β-glucan is known to be abundant in mushrooms such as Agaricus and Hanabiratake, and can be obtained by extraction. β-glucan is a compound that does not have the slimy feeling peculiar to water-soluble polymers even when blended in a large amount, has a high protective colloid property, and has a good feeling in use. However, since it is obtained by the extraction process from mushrooms, the cost is high, and β-glucan has a structure in which many glucoses are bonded through β-glycosidic bonds, so its solubility in water is low and it can be applied to cosmetics. It was difficult to blend.

  For this reason, a compound with improved water solubility by chemical modification such as carboxymethylation is also provided for β-glucan. However, when such a compound is added in a large amount, a dry touch peculiar to β-glucan cannot be obtained. .

Patent Document 1 discloses that a polysaccharide component obtained from a black yeast ( Aureobasidium pullulans ) culture solution contains β-glucan as a main component. As a composition containing β-glucan obtained by the method described in Patent Document 1, a composition containing β-glucan at a concentration of about 0.5% is the maximum concentration. It was difficult to obtain a composition containing β-glucan. Patent Document 1 discloses β-glucan obtained by drying an ethanol precipitate of a black yeast culture solution to form a powder. However, such powder is insoluble in ethanol and hardly soluble in water. Therefore, it was difficult to blend into cosmetics.

JP-A-57-149301

  An object of the present invention is to provide a β-glucan-containing composition. In particular, the present invention provides a β-glucan-containing composition that does not produce aggregates when blended into cosmetics, exhibits good protective colloid properties, improves stability, and gives a good feeling of use. The purpose is to provide.

The present invention provides a β-glucan-containing composition which is obtained by dispersing an organic solvent precipitate from a black yeast ( Aureobasidium pullulans ) culture solution in a polyhydric alcohol and substantially does not contain water. The present invention also provides a cosmetic comprising the composition. The present invention further provides a method for producing a β-glucan-containing composition characterized in that an organic solvent is added to a black yeast culture solution to obtain a precipitate, and the precipitate is dispersed in a polyhydric alcohol.

  The composition of the present invention can be blended in a relatively large amount without limiting the blending amount of other components when blended into a cosmetic. And the composition of this invention does not produce an aggregate, but exhibits the protective colloid property of β-glucan to improve the stability of the cosmetic, and can impart a good feeling to the cosmetic.

A black yeast ( Aureobasidium pullulans ) culture solution (hereinafter referred to as “black yeast culture solution”) used in the present invention is obtained by culturing Aureobasidium pullulans (black yeast) according to a conventional method, The removed culture solution. Black yeast culture fluid (Aureobasidium culture fluid) is a substance approved as a food additive, and has been conventionally incorporated into cosmetics.

For example, a method disclosed in Patent Document 1 may be employed as a black yeast culture solution preparation method. Although it does not specifically limit as a black yeast strain, The FERM-P4257 strain of patent document 1 is used suitably. A commercially available black yeast culture solution or aureobasidium culture solution may be used. Although it is not limited to these as a commercial item, For example, the culture solution of Aureobasidium pullulans FERM-P4257 is marketed under the brand name "Akphage Max" from Ikko Chemical Co., Ltd., and such a product is used suitably.

  The composition of the present invention is obtained by dispersing an organic solvent precipitate of a black yeast culture solution in a polyhydric alcohol.

  Examples of the organic solvent used for preparing the organic solvent precipitate include methanol, ethanol, isopropyl alcohol, pyridine, chloroform and the like. An organic solvent may be used independently or may be used in combination of 2 or more. Considering the application to cosmetics, ethanol and isopropyl alcohol with high safety are most preferable.

  The polyhydric alcohol in which the organic solvent precipitate is dispersed is not particularly limited as long as it is a compound having 3 or more carbons and 2 or more hydroxyl groups in the molecule and liquid at room temperature. Specifically, 1,3-propanediol, 1,3-butylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, 1,2-pentanediol, ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, glycerin, di- Examples include glycerin. Polyhydric alcohols may be used alone or in admixture of two or more.

  The composition of the present invention is prepared by adding an organic solvent to a black yeast culture solution to obtain a precipitate containing β-glucan as a main component, and adding the polyhydric alcohol to the obtained precipitate and dispersing it.

  The input amount of the organic solvent to the black yeast culture solution is not limited, and it is sufficient to input an amount by which β-glucan in the culture solution is sufficiently precipitated. Preferably, an equivalent amount to about 5 times the black yeast culture solution may be added. The time until the precipitation product after the addition of the organic solvent is obtained is not limited, and the mixture of the culture solution and the organic solvent may be allowed to stand, and it may be confirmed visually that the precipitate has settled. The precipitation operation may be repeated a plurality of times.

  The resulting precipitate is removed and the organic solvent is removed. The organic solvent may be removed by a conventional method, for example, pressing. The organic solvent does not need to be completely removed from the precipitate, and 1/2 to 5 times as much organic solvent as the dry mass of the precipitate may remain.

  The precipitate obtained above is dispersed in a polyhydric alcohol. When the precipitate is dispersed in the polyhydric alcohol, the solid content (dry weight) of the organic solvent precipitate is suitably 0.01% by weight or more, preferably 0.5% by weight or more, more preferably in the polyhydric alcohol dispersion. Is 1% by weight or more. The upper limit of the solid content of the organic solvent precipitate is 5% by weight or less, preferably 3% by weight or less. If the amount of the precipitate exceeds 5% by weight, it will be difficult to disperse in the polyhydric alcohol. The amount of polyhydric alcohol produced is too large, which is not preferable.

  In order to disperse the organic solvent precipitate in the polyhydric alcohol, any conventionally proposed method may be adopted as appropriate, and there is no particular limitation. For example, a method of using a disper, a homomixer, a planetary mixer, a kneader, or the like and dispersing using a shearing force or an impact force is preferably used.

  The composition of the present invention is substantially free of water. In the present specification and claims, “substantially free of water” means that no aqueous component is added in preparing the composition, for example, water derived from black yeast culture liquid, etc. It is not intended to exclude even moisture that is inevitably mixed during preparation.

  The composition of the present invention can increase the concentration of β-glucan compared to the products obtained in the past, and can be easily blended into an aqueous medium. A relatively large amount of β-glucan can be blended without limiting the blending amount of these components. By blending the composition of the present invention, the stability of cosmetics can be improved and a good feeling of use can be imparted by the protective colloid of β-glucan.

  The cosmetic composition containing the composition of the present invention is not particularly limited in its dosage form and product form, and is a water-in-oil type, an oil-in-water type, a water-dispersed type, a pressed form, a solid, and a powder form. can do. Product forms include facial cleansing foam, makeup remover, lotion, serum, milk, cream, massage cream, pack, makeup base, sunscreen and other skin cosmetics, powder foundation, liquid foundation, whitening powder, eye shadow, Finishing cosmetics such as eyeliner, mascara, eyebrow, blusher, concealer, lipstick, lip balm, hair shampoo, hair rinse, hair tonic, hair treatment, liquid hair conditioner, set lotion, hair spray, hair dye, etc. Examples thereof include cosmetics, powder sprays, antiperspirants such as roll-on, shaving cosmetics such as shaving foam, shaving gel, and shaving lotion.

  Among these, oil-in-water and water-dispersed products such as face-washing foams, lotions, emulsions, packs, sunscreen cosmetics, liquid foundations, hair shampoos, hair treatments, etc. are cosmetics that can easily exert the effects of the present invention.

The present invention will be described in detail below with reference to examples. However, the following examples do not limit the present invention. All blending amounts are in weight percent unless otherwise specified.

Example 1
Using the method disclosed in Patent Document 1, aureobasidium ( Aureobasidiumu pullulans FERM-P4257) was added with carbon source 0.5-1.0%, N source 0.1%, vitamins and mineral 0.1%. A black yeast culture solution obtained by culturing at a pH of 5.2 to 6.0, an aeration amount of 1/3 of the medium volume, and a temperature of 25 ° C to 30 ° C was used. After adding 300 g of ethanol to 300 g of black yeast culture and stirring, the mixture was allowed to stand for 60 minutes to precipitate β-glucan. The precipitate was filtered and removed from the solution. The operation of adding 300 g of ethanol to the taken-out precipitate again, stirring and allowing to settle by precipitation was further repeated twice, and the ethanol was gradually removed by pressing. In the process of removing ethanol, the amount of dry precipitate and the amount of ethanol were confirmed by measuring the remaining amount of drying as needed. The dry weight of the precipitate and the ethanol weight were adjusted to a ratio of 1: 2, and 9 g of a precipitate containing 3 g of the dry weight of the precipitate and 6 g of ethanol was obtained. The main component of the precipitate is β-glucan. 91 g of 1,3-propanediol (Zemea (registered trademark) propanediol: manufactured by DuPont Tate & Lyle BioProduct) was added to the resulting precipitate and dispersed uniformly with a disper (Example 1). 100 g was obtained.

Examples 2-4, Comparative Examples 1-4
Sunscreen Stabilization of the sunscreen formulation containing the composition of the present invention was evaluated. In the formulation of Table 1, a necessary amount of the component A was weighed and stirred uniformly, and then the component B was added and stirred again to be uniform. The dispersion state of the powder and the state of the composition after 30 days of standing at room temperature were visually confirmed. Moreover, the usability of the obtained sunscreen was evaluated by a monitor.

Powder dispersion state ○: The powder is uniformly dispersed.
Δ: Slight settling of powder is observed.
X: Sedimentation of powder is observed.

State of composition ○: The composition is uniform.
Δ: Slight separation is observed.
X: Separation is observed.

Usability ○: Smooth and refreshing feel.
Δ: Slightly smooth and refreshing feel.
×: Stickiness is felt.

＊アウレオバシジウムM-P04「一光化学社製」 Sunscreen formulation example

* Aureobasidium M-P04 “Ikko Chemical Co., Ltd.”

  As is apparent from the results in Table 1, it was confirmed that the composition of the present invention uniformly dispersed the powder and kept the composition stable as compared with Comparative Examples 1 to 4.

Examples 5-7, Comparative Examples 5-7
Cream formulation The usability of the cream containing the composition of the present invention was evaluated. In the formulation of Table 2, the necessary amount of the component A was weighed and dissolved by heating at 80 ° C. The component B was added to the component A and stirred uniformly with a homomixer. Thereafter, defoaming was performed to obtain a cream.

Smoothness at the time of application, no stickiness ◎: More than 8 out of 10 people recognized that it was smooth and non-sticky at the time of application.
○: Six to seven out of ten people recognized that they were smooth and non-sticky during application.
(Triangle | delta): 4-5 persons recognized that it was smooth and not sticky at the time of application | coating.
X: 3 or less out of 10 persons recognized that it was smooth and not sticky at the time of application.

Moistness at the time of application ◎: More than 8 out of 10 people recognized that it was moist at the time of application.
○: Six to seven out of ten people recognized that they were moist during application.
Δ: 4 to 5 out of 10 people recognized that they were moist during application.
X: 3 or less out of 10 persons recognized that it was moist at the time of application | coating.

Freshness after application ◎: More than 8 out of 10 people recognized that it was refreshing at the time of application.
○: Six to seven out of ten people recognized that they were refreshing during application.
(Triangle | delta): It recognized that 4-5 persons out of 10 were refreshing at the time of application | coating.
X: 3 or less out of 10 persons recognized that it was refreshing at the time of application | coating.

Emulsion state ○: Emulsion is uniform.
Δ: Slight separation is observed.
X: Separation is observed.

＊アウレオバシジウムM-P04「一光化学社製」

* Aureobasidium M-P04 “Ikko Chemical Co., Ltd.”

  As is clear from the results of Table 2, when the composition of the present invention was blended, particularly in Example 5, a cream having better usability than Comparative Examples 5 to 7 was obtained.

Example 8
Facial cleansing foam (amino acid)
A necessary amount of the component A described in Table 3 was weighed and dissolved by heating at 80 ° C. Similarly, the necessary amount of component B was weighed and dissolved by heating at 80 ° C. The component B is slowly added to the component A and stirred uniformly. Furthermore, the component C was added and stirred uniformly. After cooling to room temperature, defoaming was performed to obtain a facial cleansing foam (amino acid type).

  When the composition of the present invention is blended, foaming is good, foam quality is good, and after washing the face, a clean face-washing foam (amino acid type) is obtained despite the fact that the face is refreshed and the skin is not washed. It was.

Example 9
Facial cleansing foam (soap system)
A necessary amount of the component A described in Table 4 was weighed and dissolved by heating at 80 ° C. Similarly, the necessary amount of component B was weighed and dissolved by heating at 80 ° C. Saponification was performed by slowly adding the component B to the component A. Furthermore, the component C was added and stirred uniformly. After cooling to room temperature, defoaming was performed to obtain a face-washing foam (soap system).

  When the composition of the present invention is blended, foaming, foam quality is good, and after washing the face, a clean face-washing foam (soap-type) is obtained, regardless of whether the face is refreshed and clean. It was.

Example 10
Lotion Toner A necessary amount of the component A described in Table 5 is weighed and stirred uniformly. The component B was added to the component A and stirred uniformly. Furthermore, the component C was added and stirred uniformly to obtain a skin lotion. The lotion was prepared at room temperature.

＊ビオセラG 「大日本化成社製」：スフィンゴモナスエキス、グリセリン、天然ビタミンE

* Biosera G "Dainippon Kasei Co., Ltd.": Sphingomonas extract, glycerin, natural vitamin E

  When the composition of the present invention was blended, a refreshing use feeling was obtained at the time of application and after application, and a lotion having no stickiness was obtained.

Example 11
Emulsion A necessary amount of the component A described in Table 6 was weighed and dissolved by heating at 80 ° C. Similarly, the necessary amount of component B was weighed and stirred uniformly. The component A was added to the component B and stirred uniformly. Thereafter, defoaming was performed to obtain an emulsion.

＊クインスシード2SP「大日本化成社製」：クインスシードエキス、1,3-ブチレングリコール、フェノキシエタノール、精製水

* Quince Seed 2SP "Dainippon Kasei Co., Ltd.": Quince Seed Extract, 1,3-butylene glycol, phenoxyethanol, purified water

  When the composition of the present invention was blended, the stability of the emulsion with time was good, and a refreshing feel during use and after application was obtained, and an emulsion without stickiness was obtained.

Example 12
Pack A necessary amount of the component A described in Table 7 was weighed and dissolved by heating at 80 ° C. Similarly, the necessary amount of component B was weighed and stirred uniformly. The component A was added to the component B and stirred uniformly. Then, it degas | defoamed and obtained the pack.

  The pack containing the composition of the present invention gave a smooth feel when applied, and a refreshing feel with no stickiness after peeling of the pack.

Example 13
Sunscreen cream A necessary amount of the component A described in Table 8 was weighed, heated and dissolved at 80 ° C., and stirred uniformly. A necessary amount of the component B was weighed and added to the component A and stirred uniformly. Furthermore, component C was added and stirred uniformly with a homomixer. After cooling to room temperature, defoaming was performed to obtain a sunscreen cream.

  By blending the composition of the present invention, a refreshing feel was obtained, a dispersion stability of the fine particle titanium oxide was improved, and a sunscreen excellent in water resistance was obtained.

Example 14
Foundation A necessary amount of the component A described in Table 9 was weighed, dissolved by heating at 80 ° C., and stirred uniformly. A necessary amount of the component B was weighed and added to the component A and stirred uniformly. Furthermore, component C was added and stirred uniformly with a homomixer. After cooling to room temperature, defoaming was performed to obtain a foundation.

  When the composition of the present invention was blended, a refreshing feel was obtained, the dispersion stability of the powder was improved, and a smooth finished foundation was obtained with an appropriate covering power.

Example 15
Hair shampoo A necessary amount of the component A described in Table 10 was weighed, heated and dissolved at 80 ° C., and stirred uniformly. The component B was added to the component A and stirred uniformly. After cooling to room temperature, a hair shampoo was obtained.

  When the composition of the present invention was blended, a cleansing hair shampoo with good foaming and foam quality was obtained.

Example 16
Hair Treatment A necessary amount of the component A described in Table 11 was weighed, heated and dissolved at 80 ° C., and stirred uniformly. Similarly, the necessary amount of component B was weighed and stirred uniformly. The component A was added to the component B and stirred uniformly. After cooling to room temperature, defoaming was performed to obtain a hair treatment.

  By blending the composition of the present invention, a hair treatment that gives good smoothness and softness to the hair, and also a hair-merging effect was obtained.

Claims (12)

A composition containing β-glucan and containing substantially no water, wherein the organic solvent precipitate of black yeast ( Aureobasidium pullulans ) culture solution is dispersed in polyhydric alcohol without drying . The composition according to claim 1, wherein the solid content of the organic solvent precipitate is 0.01 to 5% by weight of the total. The composition according to claim 1 or 2, wherein the organic solvent is selected from methanol, ethanol, isopropyl alcohol, pyridine and chloroform. The composition according to claim 3, wherein the organic solvent is ethanol or isopropyl alcohol. The composition according to any one of claims 1 to 4, wherein the polyhydric alcohol is a compound having 3 or more carbons and 2 or more hydroxyl groups in the molecule and liquid at normal temperature. The composition according to any one of claims 1 to 5, wherein the polyhydric alcohol is 1,3-propanediol. The composition according to any one of claims 1 to 6, which is used for producing a cosmetic containing water. The composition according to claim 7, which is used for producing an oil-in-water type or water-dispersed cosmetic . Cosmetics which mix | blend the composition in any one of Claims 1-8 . The cosmetic according to claim 9, comprising water. The cosmetic according to claim 10, which is an oil-in-water type or water-dispersed type cosmetic. The preparation method of the composition in any one of Claims 1-8 which has the process of throwing an organic solvent into a black yeast culture liquid, obtaining a precipitate, and the process of disperse | distributing this precipitate in a polyhydric alcohol.