JP5486501B2 - 小さなｒｎａ分子の改善された検出のための方法、組成物およびキット - Google Patents

小さなｒｎａ分子の改善された検出のための方法、組成物およびキット Download PDF

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JP5486501B2
JP5486501B2 JP2010528992A JP2010528992A JP5486501B2 JP 5486501 B2 JP5486501 B2 JP 5486501B2 JP 2010528992 A JP2010528992 A JP 2010528992A JP 2010528992 A JP2010528992 A JP 2010528992A JP 5486501 B2 JP5486501 B2 JP 5486501B2
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target sequence
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primer
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JP2011501663A (ja
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イゴール バシリー クートヤビン，
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/207—Modifications characterised by siRNA, miRNA

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  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010528992A 2007-10-09 2008-10-08 小さなｒｎａ分子の改善された検出のための方法、組成物およびキット Active JP5486501B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97865707P 2007-10-09 2007-10-09
US60/978,657 2007-10-09
PCT/US2008/079169 WO2009048928A1 (en) 2007-10-09 2008-10-08 Methods, compositions and kits for the improved detection of small rna molecules

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JP2011501663A JP2011501663A (ja) 2011-01-13
JP2011501663A5 JP2011501663A5 (OSRAM) 2012-11-22
JP5486501B2 true JP5486501B2 (ja) 2014-05-07

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JP2010528992A Active JP5486501B2 (ja) 2007-10-09 2008-10-08 小さなｒｎａ分子の改善された検出のための方法、組成物およびキット

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US (2) US8637276B2 (OSRAM)
EP (1) EP2198059B1 (OSRAM)
JP (1) JP5486501B2 (OSRAM)
AU (1) AU2008310955B2 (OSRAM)
CA (1) CA2702584A1 (OSRAM)
ES (1) ES2526925T3 (OSRAM)
WO (1) WO2009048928A1 (OSRAM)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008310955B2 (en) * 2007-10-09 2015-01-29 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules
US9074248B1 (en) * 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
WO2016129941A1 (ko) 2015-02-09 2016-08-18 주식회사 하임바이오텍 마이크로알앤에이 검출용 키트 및 방법
WO2017087943A1 (en) * 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
JP6970732B2 (ja) * 2016-07-21 2021-11-24 ハイムバイオテック インコーポレイテッド Ｒｎａ検出用キット及び方法
JP7055691B2 (ja) * 2017-07-11 2022-04-18 株式会社東芝 短鎖核酸伸長用プライマーセット、アッセイキット、短鎖核酸伸長方法、増幅方法及び検出方法
US20220389497A1 (en) * 2019-11-04 2022-12-08 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN118166080B (zh) * 2024-04-15 2025-04-04 苏州市立医院 一种无RNA逆转录酶的miRNA一步法检测方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218118B1 (en) * 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
CA2401781A1 (en) * 2000-03-01 2001-09-07 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
DK1735459T3 (da) 2004-04-07 2012-05-29 Exiqon As Fremgangsmåder til kvantificering af microRNA'er og små interfererende RNA'er
WO2007127992A2 (en) * 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
AU2008310955B2 (en) * 2007-10-09 2015-01-29 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules

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US20140170666A1 (en) 2014-06-19
AU2008310955A1 (en) 2009-04-16
AU2008310955B2 (en) 2015-01-29
ES2526925T3 (es) 2015-01-16
WO2009048928A1 (en) 2009-04-16
US20100304442A1 (en) 2010-12-02
JP2011501663A (ja) 2011-01-13
US8980558B2 (en) 2015-03-17
US8637276B2 (en) 2014-01-28
CA2702584A1 (en) 2009-04-16
EP2198059B1 (en) 2014-12-03
EP2198059A1 (en) 2010-06-23

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