JP5429562B2 - Beverage with increased collagen activity containing fermented rosemary extract - Google Patents

Description

The present invention relates to a beverage exhibiting an action of increasing collagen, comprising a fermented rosemary extract obtained by fermenting rosemary, ceramide and placenta.

Collagen is a protein present in skin, bone, cartilage, blood vessels, and membrane tissues in various tissues. The number of types of collagen is 10 or more, and its function is based on the structure of the skeleton of the parenchyma, bone, and inflammation. Such as prevention of cancer invasion and prevention of metastasis.

Fibroblasts produce collagen, and the production involves fibroblast proliferation stimulation and growth factors, and activation of transcription factors induces collagen production at the gene level.

Living organisms are decomposed into collagen or peptides by the enzymes that degrade collagen, collagenase or matrix metalloprotease. This collagenase is induced and activated during inflammation and by cancer cells.

In addition, even with arthritis, collagenase is produced by inflamed tissues such as cartilage sites and surrounding muscles, and inflammatory cells such as neutrophils, causing collagen to break down and dissipating collagen, resulting in tissues such as muscles and bones. Collapses.

As a method of increasing collagen, there are a method of increasing collagen production of fibroblasts and inhibition of collagenase activity or suppression of induction, etc. It is preferable to use both methods in combination, extraction of raw materials and active ingredients having both functions Research and development of methods are underway.

Methods for increasing collagen, drugs, foods, and cosmetics include cancer cell metastasis, suppression of angiogenesis related to cancer metastasis, suppression of cancer cell proliferation, suppression of arthritis, improvement of inflammation, suppression of osteoporosis, suppression of cellulite, Since it is effective for improvement, improvement and prevention of wrinkles and talmi, and has high industrial applicability, methods for increasing collagen in various research facilities have been studied.

Plants and herbs contain active ingredients that increase collagen, and these active ingredients are being isolated and purified.

Although anti-inflammatory agents such as fenbuchen, indomethacin, and ipflofen are known as chemically synthesized collagenase inhibitors, they have been reported to have side effects such as dry cough and gastrointestinal tract, respectively. It is desired.

As an invention for increasing collagen, there is a composition containing a phenol compound derived from mimosa, and there is a composition comprising a biologically active phenol compound-containing extract or its active fraction obtained from white crab (for example, , See Patent Document 1).

As an invention of a substance that inhibits collagenase, there is a collagenase activity inhibitor containing at least one of magnolol and honokiol as an active ingredient, and here, it includes parts of honoki leaves, bark, trunk material, root bark and the like. Components are used (for example, see Patent Document 2).

In addition, as herbs and plant-derived terpenes, there is an invention regarding the use of at least one saponin or sapogenol as a cosmetic agent for increasing collagen in the dermis-epidermal junction (see, for example, Patent Document 3).

However, all the inventions are caused by either the enhancement of collagen production or the inhibition of collagenase, the effects thereof are limited, and the industrial application is limited.

Japanese Patent No. 3626727 Japanese Patent No. 2886623 Special table 2002-516837

As described above, a chemically synthesized anti-inflammatory agent exhibiting a collagenase inhibitory action has a problem that side effects such as dry cough and gastrointestinal tract disorders occur. Furthermore, there is a problem that even if collagen is fed as it is, it is not converted into collagen of its own skin or joints.

On the other hand, there is a problem that although the safety of a naturally derived substance is high, its effect is mild. Accordingly, foods, particularly beverages, containing natural product-derived substances exhibiting a collagen-increasing action with weak side effects and excellent anti-inflammatory effects are desired.

The present invention has been made paying attention to the problems existing in the prior art as described above. The object is to provide an excellent beverage with weak side effects, that is, a beverage exhibiting an action of increasing collagen containing a rosemary fermented extract.

In order to achieve the above object, the invention described in claim 1 relates to a beverage exhibiting an action of increasing collagen containing a rosemary fermented extract, ceramide and placenta.

Since this invention is comprised as mentioned above, there exist the following effects. According to the beverage containing the rosemary fermented extract according to claim 1, there are few side effects, a collagen increasing action can be exhibited, and the skin can be made beautiful.

Hereinafter, the best mode for carrying out the present invention will be described in detail.

First, the beverage according to the present embodiment contains rosemary powder, soybeans, natto bacteria, and fermented fermented liquor with magnesium-type ceramic beads. 1% by weight, further containing 0.004% to 0.4% by weight of ceramide and 0.03% to 3% by weight of placenta, exhibiting a collagen increasing action.

The rosemary fermented extract used here exhibits a collagen increasing action. This extract is obtained by fermenting rosemary with natto bacteria and then treating the fermented fermented liquid with magnesium-type ceramic beads.

Rosemary fermented extract is rich in terpenoids and peptides, and these components activate collagen synthase to promote collagen production.

This method for producing a rosemary fermented extract is characterized by adding a rosemary powder, soybeans and natto bacteria, and treating the fermented fermented liquid with magnesium-type ceramic beads.

Below, the manufacturing method of a rosemary fermented extract is demonstrated.

Rosemary as a raw material has the scientific name Rosmarinus officinalis, and can be used from any of Japan, China, Europe such as Spain, and the United States. Rosemary cultivated with pesticide-free or reduced pesticides is preferred.

The terpenoids are contained in rosemary, and in particular, the content of diterpene is high and is 10 to 13% by dry weight, which is preferable as a raw material for the intended rosemary fermented extract. In this production process, rosemary leaves are washed with water, dried, and then pulverized with a pulverizer. Thereafter, it is sterilized by autoclave for the purpose of preventing propagation of various bacteria.

The soybean used as a raw material can be used in soybeans of any origin such as Japanese, Chinese, American and Russian. Non-genetically modified soybeans cultivated with pesticide-free or reduced pesticides are preferred. This soybean is pulverized by a pulverizer such as DM-6 manufactured by Nakayama Technical Research Institute. Thereafter, it is sterilized by autoclave for the purpose of preventing propagation of various bacteria.

The Bacillus natto used as a raw material is a kind of Bacillus subtilis used for natto and food processing. Natto bacteria manufactured by Natto Motopo are preferred because of their stable quality and suitable for fermentation.

The amount of the raw material used for the fermentation is preferably 0.5 to 3 wt. For soybean powder and 0.001 to 0.02 wt.

The fermentation is carried out in a clean culture tank, and it is preferable to mix the materials with tap water.

Moreover, this fermentation is heated at 30-42 degreeC, and fermentation is performed for 24-92 hours. In order to extract efficiently after fermentation, it is diluted with tap water.

The terpenes in rosemary are liberated by this fermentation process. In addition, protein derived from soybean is decomposed by protease to produce a peptide. Furthermore, terpenes and peptides are bound by transesterification enzyme generated in the fermentation process to form an ester body.

By this fermentation process, terpene, peptide, or the like is released or combined to produce a component that exhibits a collagen producing action.

It is preferable that the fermented product produced by the above fermentation is extracted with hot water of 30 to 60 ° C. because the product can be efficiently recovered and the next step can be easily performed.

It is preferable to concentrate the obtained fermented product by freeze-drying or the like.

The resulting fermented product is treated with magnesium type ceramic beads. Magnesium-type ceramic beads may be manufactured in Japan or in the USA. For example, magnesium type ceramic beads manufactured by Cytokin Co., Ltd. are preferable.

By treating the fermented product with magnesium type ceramic beads, magnesium donates electrons and terpenes and peptides are stabilized through the ceramic surface.

As for the addition amount of a magnesium type ceramic bead, 0.01 to 0.1 weight is preferable with respect to 1 weight of said fermented material.

It is preferable to use magnesium-type ceramic beads that have been washed with deionized water, sterilized by autoclaving at 121 ° C. for 20 minutes, and then cooled.

Putting the fermented product in a clean tank, adding magnesium-type ceramic beads, heating to 35 ° C to 40 ° C, stirring and reacting 2-10 times per minute, It is preferable because it can be efficiently performed.

The obtained reaction solution is filtered with a clean filter paper and sterilized to obtain the intended rosemary fermented extract as a liquid.

This liquid is dried with an evaporator or a vacuum dryer, and the water is removed to obtain a powder.

The beverage containing the rosemary fermented extract thus obtained exhibits an increasing action of collagen.

The target beverage that increases collagen is 0.1% to 1% by weight of the above-mentioned fermented rosemary extract, 0.004% to 0.4% by weight of ceramide and 0.03% by weight of placenta. Contains ~ 3 wt%.

The rosemary fermented extract used here activates collagen synthase to promote collagen production. Ceramide also promotes collagen production by protecting skin cells, bone cells and fibroblast cell membranes. Furthermore, collagen production is maintained by preventing the degradation of collagen formed in the skin and tissues.

Placenta promotes collagen production by supplying amino acids, especially proline, to produce collagen. It also strengthens the tissue by increasing hyaluronic acid.

Since the production of collagen synergistically increases by combining rosemary fermented extract, ceramide and placenta, it is necessary to add rosemary fermented extract, ceramide and placenta simultaneously.

When the content of the rosemary fermented extract is less than 0.1% by weight, the productivity of collagen is not sufficiently exhibited. On the other hand, when the content of the rosemary fermented extract exceeds 1% by weight, there is a disadvantage that the cost is high and industrial use is limited.

When the ceramide of the beverage is less than 0.004% by weight, the permeability of the ceramide to the cell membrane is not sufficiently exhibited, and the protective effect of the cells is lowered. On the other hand, when ceramide exceeds 0.4% by weight, the fat solubility becomes high, and oil and water are separated from each other in the beverage, and the quality is deteriorated.

If the placenta is less than 0.03% by weight, the amino acids forming the collagen are insufficient, and the production of collagen may not be sufficient. On the other hand, if the placenta exceeds 3% by weight, the unique odor becomes strong and the palatability may decrease.

It is preferable that the beverage is put in a glass bottle, a PET bottle, an aluminum can or the like and sterilized from the viewpoint of maintaining the collagen increasing action.

Since the said drink exhibits a collagen increase effect | action, it is utilized as a cosmetic drink for the purpose of the increase in the collagen of skin.

The above beverages are suitable for those who have suffered skin disorders such as acne and atopic dermatitis, because they activate wound fibroblasts to repair wounds and disorders.

Since the beverage increases collagen such as joints, it is used for the purpose of treating or preventing osteoporosis due to the elderly or menopause.

It is preferable that the beverage is used as a beverage for a person who has damaged the spinal cord or spinal column due to an accident because it promotes the repairing action of the disorder.

Hereinafter, the embodiment will be specifically described with reference to examples and test examples.

Rosemary leaves grown in Hokkaido were harvested, washed with water and dried in the sun. This was pulverized by a pulverizer (high-speed pulverizer, manufactured by Nihon Leeing).

1 kg of this crushed rosemary leaf was added to a clean 50 liter fermentation tank, and 5 liters of purified water was added.

A ground soybean from Hokkaido was purchased from Kongo Pharmaceutical Co., Ltd., and 1 kg of the ground soybean was added to the fermentation tank and stirred.

This was heated to 95 ° C., cooled and sterilized.

To this, 10 g of natto bacteria from Natto Honpo Co., Ltd. was added and fermented. This fermentation was carried out at 40 ° C. for 72 hours.

The obtained fermented product was filtered with a cloth and further filtered with a clean filter paper to collect a fermentation broth. This was put into a clean stainless steel tank having a capacity of 10 liters.

To 5 kg of the above fermented liquid, 250 g of magnesium type ceramic beads manufactured by Cytokin Co., Ltd. was autoclaved and added to the fermented liquid tank.

The tank was heated to 37 ° C. and reacted while stirring at 5 revolutions per minute. The resulting reaction solution was filtered through clean filter paper.

The obtained filtrate was autoclaved to obtain a target rosemary fermented extract solution.

100 g of the rosemary fermented extract was put into a clean tank, and 50 L of purified water was added. To this, 40 g of ceramide derived from rice bran (manufactured by Kongo Pharmaceutical) was added and heated to 30 ° C. to dissolve.

Furthermore, 300 g of porcine placenta (manufactured by Toyo Enzyme Chemical) was added and dispersed. Here, 1.3 kg of reduced starch syrup and 130 g of citric acid were added and dissolved.

Purified water was added to make the total volume 100 L, and the mixture was stirred with a stirrer. This was filled into a 50 mL glass container and capped.

This beverage was sterilized at 85 ° C. for 10 minutes, mixed by inversion, and then cooled to obtain the sample of Example 1. Moreover, the drink which added only rosemary fermented extract, ceramide, and placenta was similarly manufactured, and it used for the following tests.

Below, the test example using the drink of the said Example 1 is reported.

The effect was tested by experiments using human skin-derived fibroblasts. This is a test method for evaluating the amount of collagen produced using human skin-derived fibroblasts, and has been established as an evaluation method for human skin.
(Test Example 1)

Normal human adult-derived dermal fibroblasts (Fibrocell NHDF (AD), manufactured by Kurabo Corp.) are cultured in a dedicated culture solution, and the sample obtained in Example 1 is dissolved in the culture solution, and 0.1 mg / mL It added so that it might become a density | concentration, and it culture | cultivated at 37 degreeC for 48 hours.

Dimethyl sulfoxide was used as a solvent control. As a positive control, fibroblast growth factor (FGF, manufactured by Cosmo Bio) was dissolved in purified water and Sample 1 was added at a concentration of 1 mg / mL. The number of cells was counted, and the amount of collagen type 1 was observed by an antibody method (manufactured by Chondrex).

As a result, the number of cells by addition of the sample of Example 1 was 131% compared to the value of the solvent control with respect to the value of the solvent control, and the sample of Example 1 showed fibroblast proliferation.

On the other hand, the beverage to which only the rosemary fermented extract was added was 105% compared to the solvent control value. The beverage to which only ceramide was added was 103% compared to the solvent control value. The beverage to which only placenta was added was 104% compared to the value of the solvent control, and the increase rate was slight compared to the sample 1.

In addition, the addition of 1 mg / mL of the positive control FGF was 120% with respect to the value of the solvent control. Therefore, the sample of Example 1 may exhibit a significant proliferation of fibroblasts than FGF. found.

With respect to the amount of collagen, the value of the 1 mg / mL sample in Example 1 was 144% of the value of the solvent control compared to the solvent control, indicating an increasing action of collagen.

On the other hand, the beverage to which only the rosemary fermented extract was added was 109% compared to the solvent control value. The beverage added with ceramide alone was 108% of the solvent control value. The beverage to which only the placenta was added was 107% compared to the value of the solvent control, and the increase rate was smaller than that of the sample 1.

In addition, the addition of 1 mg / mL of the positive control FGF was 130% with respect to the value of the solvent control. Thus, it was found that the sample of Example 1 exhibited a stronger collagen increasing action than FGF.

Beverages exhibiting an action of increasing collagen containing the rosemary fermented extract of the present invention contribute to the health and beauty of the nation by promoting the production of collagen. It also contributes to the development of the beauty industry.

Furthermore, since collagen is required for cartilage formation and bone remodeling, this beverage is used for the treatment of joint pain and age-related osteoporosis due to diseases and accidents, and contributes to the health promotion of the elderly. .

The present invention utilizes fermentation technology and contributes to technical improvement in the fermentation industry.

Claims (1)

Rosemary powder obtained by adding rosemary powder, soybeans, natto bacteria, and fermented fermented liquor with magnesium-type ceramic beads 0.1% to 1% by weight, and 0% ceramide A beverage exhibiting an action of increasing collagen, comprising .004 wt% to 0.4 wt% and placenta 0.03 wt% to 3 wt%.