KR20210064027A - Method for manufacturing mucin compelx extract for skin recovery based on multiple fermentation processes - Google Patents

Abstract

Disclosed is a method for producing a mucin complex extract using multiple fermentation processes. The method for producing a mucin complex extract according to various embodiments of the present invention comprises the steps of: performing ozone treatment of snails; performing primary fermentation of ozone-treated snails using at least one enzyme from the first group of enzymes; and performing secondary fermentation of the primary fermented snails using at least one enzyme of the second group of enzymes, wherein the enzymes used in the primary fermentation step and the secondary fermentation step are different enzymes.

Description

Manufacturing method of mucin complex extract for skin recovery using multiple fermentation processes {METHOD FOR MANUFACTURING MUCIN COMPELX EXTRACT FOR SKIN RECOVERY BASED ON MULTIPLE FERMENTATION PROCESSES}

Various embodiments of the present invention relate to a method for extracting and preparing a mucin component having a high absorption rate in a high content from snails.

Recently, the word 'inner beauty' has appeared. Inner beauty refers to all cosmetics that you eat, meaning that you take care of healthy skin from the inside out. Natural ingredients such as chitosan and nuts are used as raw materials for Inner Beauty, and recently, products using mucin extracted from hemp, eel, and snails are known.

The main components of snails, mucopolysaccharide protein and chondroitin sulfate component (mucopolysaccharide), are effective in treating adult diseases, promoting growth and preventing skin aging, nourishing and strengthening, whitening, and beauty, and their efficacy is mentioned in Donguibogam, etc. . The mucin component including the mucopolysaccharide protein and chondroitin sulfur component can be used as various raw materials such as cosmetics, food, health juice or extract.

In this regard, Korean Patent Laid-Open Publication No. 1995-0002741 (published on February 16, 1995) discloses a method for manufacturing cosmetics using a mucin component extracted from a snail.

However, in the patent document, since the snail extract is prepared by simply boiling or steaming the snail, the core component is damaged by heat, and the mucopolysaccharide component is evaporated along with the evaporation of water. .

Mucin, a component of mucopolysaccharide, acts as a messenger of regeneration information between cells, and is known as a component having the function of promoting skin dermal growth factor and fibroblasts. This mechanism is a method of cell therapy for deep wrinkles or burns, and it can be confirmed that the speed of skin recovery is significantly faster than that of the control group in various SCI-level papers.

Various embodiments of the present invention aim to provide a method for extracting a mucin complex extract with high efficiency and high absorption from edible snails.

In addition, through the mucin complex extract prepared through various embodiments of the present invention, functional efficacy effect research and health functional food raw material development on increasing the skin recovery rate based on intake and application can be carried out.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

A method for producing a mucin complex extract according to various embodiments of the present invention for solving the above-described problems comprises the steps of ozone treatment of snails; Primary fermentation of the ozone-treated snails using at least one enzyme of the first group of enzymes; and secondary fermentation of the primary fermented snails using at least one enzyme of a second group of enzymes, wherein the enzymes used in the primary fermentation stage and the secondary fermentation stage are different enzymes. can be characterized.

In some embodiments, the first group enzyme may be Escherichia coli, Propionibacterium, Pediococcus, HE (Hericium erinaceum Mycelium), Lactococcus, Streptococcus and Saccharomyces cerevisiae.

In some embodiments, the group 2 enzyme is Ent. Faecium, Breve, Longum, Leuconostoc mesenteroides, Saccharomyces cerevisiae, Clostridium butyricum, Enterobacter, Klebsiella, Lactobacillus plantarum and Escherichia coli.

In some embodiments, the primary fermentation step may include culturing the snail in a solid state.

In some embodiments, the solid culturing step may include mixing sucrose with the snail sample mixed with the primary enzyme and culturing at a temperature of 20-50° C. for 10-20 days.

In some embodiments, the method may further include drying the cultured snail sample at a temperature of 60 to 70° C. for 8 to 12 hours.

In some embodiments, the second fermentation step comprises: (a) mixing at least one enzyme of the second group enzyme and distilled water with the first fermented snail; and (b) fermenting the mixed snail sample at 25-40° C. for 5-10 days.

In some embodiments, the secondary fermentation step may further include (c) sterilizing the fermented snail sample at a temperature of 90 to 120° C. for 3 to 10 minutes.

In some embodiments, the secondary fermentation step may further include (d) cooling the sterilized snail sample to 30° C. or less.

In some embodiments, the secondary fermentation step, (e) filtering the cooled snail sample to a size of 400 ~ 1000mesh; and (f) concentrating the filtered snail sample.

According to an embodiment of the present invention, since the mucin complex extract of the snail can be extracted with higher efficiency, it can be used more economically and efficiently as a functional raw material for food, cosmetics, or various products.

In addition, the mucin component extracted according to an embodiment of the present invention can be easily stored and processed into a formulation that is easy to utilize for additional functional research.

Furthermore, the mucin component molecules extracted according to an embodiment of the present invention are decomposed by the fermentation process to have a higher body absorption rate.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 to 3 are flowcharts of a method for extracting snail mucin according to an embodiment of the present invention.
4 is experimental data comparing snail extract components according to the fermentation process.
5 is a skin test result after ingestion of a beverage containing a mucin complex extract.

The terms used in the present specification are for describing exemplary embodiments and are not intended to limit the present invention. In this specification, the singular form also includes the plural form unless specifically stated in the phrase. As used herein, “comprises” and/or “comprising” do not exclude the presence or addition of one or more other elements other than the mentioned elements. Throughout the specification, the same reference numerals refer to the same elements, and "and/or" includes each and all combinations of one or more of the mentioned elements. Although "first", "second", and the like are used to describe various elements, it goes without saying that these elements are not limited by these terms. These terms are only used to distinguish one component from another component. Therefore, it goes without saying that the first component mentioned below may be the second component within the technical idea of the present invention.

The 'mucin complex extract' referred to in this document may be interpreted as referring to a complex such as mucin or a glycoprotein containing mucin. In this document, 'mucin complex extract' may be replaced with 'mucin component'.

1 to 3 are flowcharts of a method for obtaining a snail mucin complex extract according to an embodiment of the present invention.

According to one embodiment, the method for producing a snail mucin complex extract may include a snail ozonation step (S101), a primary fermentation step (S103), and a secondary fermentation step (S105). Each of the steps disclosed in FIG. 1 may be performed by omitting at least some or changing the mutual order.

First, in the snail ozone treatment step S101, ultraviolet rays of a predetermined wavelength range may be irradiated to the snail of a predetermined capacity through an ozone treatment unit such as an ozone lamp. The ozone treatment unit may be configured to be irradiated with ultraviolet rays of a specific wavelength, for example, a wavelength band of 100 nm to 500 nm, but the embodiment of the present invention does not limit the type of ozone treatment or a specific wavelength region of the ozone lamp.

Microorganisms contained in snails can be purified and sterilized by the strong oxidative decomposition of ozone.

On the other hand, prior to the ozone treatment step, a sorting operation, washing and pulverizing operations for the snails may be performed. However, the pulverization of snails is not necessarily preceded, and the pulverization of snails may be performed not only before the ozone treatment step but also at a specific time after the ozone treatment step.

Next, the primary fermentation step (S103) for the snail proceeds.

2, the primary fermentation step (S103) includes a solid culture step (S201) and a drying step (S203).

Solid culture is a process of cultivating microorganisms or cells of animals and plants on a solid medium, and allows the cells of snails to continuously divide.

To this end, at least one enzyme of the first group of enzymes and sucrose may be mixed and used for culturing a snail sample. According to one embodiment, the first group enzymes may include Escherichia coli, Propionibacterium, Pediococcus, HE (Hericium erinaceum Mycelium), Lactococcus, Streptococcus and Saccharomyces cerevisiae. These first group enzymes were included as the most optimal enzymes from which active amino acid components were extracted from snails by an experimental method.

According to an embodiment, the first group enzyme may be used in a mixed state with distilled water at a concentration of about 5 to 15% (v/v), and the mixed solution is cultured at a temperature of 20 to 50° C. for 10 to 30 days. can be

When the solid culture step (S201) is completed, the drying step (S203) proceeds.

According to one embodiment, in the drying step, the cultured snail sample may be dried at a temperature of 60 to 70° C. for 8 to 12 hours. Thereby, a primary fermentation extract is obtained while the residue of the cultured snail sample is removed.

Each step in FIG. 2 is not necessarily included in the primary fermentation step (S103). According to various embodiments, the solid culture step (S201) and the drying step (S203) in FIG. 2 may be at least partially formed as a separate process from the primary fermentation step (S103).

Next, the secondary fermentation step (S105) is performed with respect to the primary fermented extract.

Referring to FIG. 3 , the secondary fermentation step (S105) may include a mixing step (S301), a sterilization step (S303), a cooling step (S305), and a filtration and concentration step (S307).

In the mixing step (S301), at least one of the group 2 enzymes is diluted in distilled water and mixed with the primary fermented extract. According to one embodiment, after mixing at least one enzyme selected from the group 2 enzymes at a concentration of 10% (v/v) based on 1L of distilled water, it may be mixed again with the primary fermented extract.

According to one embodiment, the group 2 enzyme is Ent. Faecium, Breve, Longum, Leuconostoc mesenteroides, Saccharomyces cerevisiae, Clostridium butyricum, Enterobacter, Klebsiella, Lactobacillus plantarum and Escherichia coli. These second group enzymes were included as the most optimal enzymes from which active amino acid components were extracted from snails by an experimental method.

Next, the sample in which the primary fermentation extract, at least one of the second group enzymes, and distilled water are mixed may be fermented in a low temperature incubator (eg, B.O.D incubator) at a temperature of 25 to 40° C. for 5 to 10 days.

Then, the step of sterilizing the fermented snail sample (S303) proceeds. This sterilization step, for example, may be sterilized at a temperature of 90 ~ 120 ℃ for 3 to 10 minutes.

After the sterilized snail sample is cooled at a temperature of 30° C. or less (S307), it is filtered and concentrated to a size of 400 to 1000 mesh. Thereby, a secondary fermented extract of snails is produced.

Although the sterilization step (S303), cooling step (S305), filtration and concentration step (S307) disclosed in FIG. 3 are disclosed as a specific process of secondary fermentation together with the fermentation step (S301) by the second group enzyme, various According to an embodiment, the steps S303, S305 and S307 may be configured as separate and independent steps from the step S301.

The active ingredients extracted by the above-described embodiments of FIGS. 1 to 3 are shown in FIG. 4 . As shown, it can be confirmed that the component of the effective amino acid is higher by going through the primary fermentation and the secondary fermentation compared to the general process of mucin extraction (a process that does not go through the fermentation process at all). In this case, the enzyme used in the primary fermentation and the enzyme used in the secondary fermentation are different enzymes.

In this case, the general process compared in the data of FIG. 4 may proceed as follows.

After washing 1.000g of 5-month-old Acatina furica snails, pulverize them to a size of 300~500mesh, and ozone treatment is carried out for a predetermined time by an ozone lamp. Next, the ozonated snails are subjected to hydrothermal treatment through a hydrothermal device to obtain a mucin complex extract from the snail sample.

The hot water extraction process may be performed in a device such as an extruder that can control internal pressure and temperature conditions, which may be done in a device of direct pressure or indirect pressure. The hydrothermal treatment step is carried out at a temperature of 100 to 130° C. for 9 to 16 hours, and is performed at a pressure of 10,000 Pa or more and 40,000 Pa.

After the hot water extraction process, a filter filtration process to additionally remove foreign substances may be performed, and the extracted mucin complex extract may be filled in a container corresponding to each product, and then quality inspection may be performed.

The following are examples of each including primary and secondary fermentation processes.

<Example 1>

After washing 1.000 g of 5-month-old Acatina furica snails, pulverized to a size of 300-500 mesh, ozone treatment was performed for about 15 by an ozone lamp. At least one of Escherichia coli, Propionibacterium, Pediococcus, HE (Hericium erinaceum Mycelium), Lactococcus, Streptococcus and Saccharomyces cerevisiae was selected for ozone-treated snails or mixed with a stirred solution of 15 cc and 20 g of sucrose. In this case, at this time, the fermentation solution of 15cc is diluted to a concentration of 10% (v/v) in distilled water. The mixed solution is incubated in a solid medium at a temperature of 25° C. for 20 days. Then, the cultured snail sample was dried at a temperature of 65° C. for 10 hours to obtain a primary fermented extract containing a mucin complex extract.

<Example 2>

* For the primary fermented extract obtained in Example 1 above, Ent. At least one of Faecium, Breve, Longum, Leuconostoc mesenteroides, Saccharomyces cerevisiae, Clostridium butyricum, Enterobacter, Klebsiella, Lactobacillus plantarum and Escherichia coli was selected or mixed with a stirred 15cc solution. At this time, the fermentation solution of 15cc was diluted to a concentration of 10% (v/v) in 1L of boiling water, and the mixed solution was fermented at a temperature of 30° C. in a low temperature incubator (B.O.D. incubator) for 5 days. Then, the fermented solution was sterilized at a temperature of 100° C. for 5 minutes, the sterilized solution was cooled at a temperature of 20° C., and then filtered to a size of 500 mesh and concentrated to obtain a secondary fermented extract.

Experimental data as shown in FIG. 4 was extracted according to Examples 1 and 2 as described above. 4A is experimental data obtained by selectively stirring each of the first group enzyme and the second group enzyme several times in each fermentation step, and FIG. 4B is the first group enzyme using only a specific enzyme as the primary fermentation enzyme. and three enzymes in group 2 enzymes were used as secondary fermentation enzymes.

In the above-described examples, the effective amino acids were Aspanic Acid, Glutanic Acid, Serine, Glycine, Histidine, Arginine, Threonine, Proline, Tyrosine, Valine, Methonine, Cysteine, Leucin, Phenylalanine, Asparagine, Lysine, Tryptephane.

In addition, in the above-described embodiments, washing, pulverization, hydrothermal treatment, etc. described in the general process may be preceded prior to the snail ozone step.

<Experimental Example>

The experimental example below is a self-test result of skin improvement effect through ingestion of mucin complex extract on 30 women in their 30s, 40s, and 50s.

Experimental purpose

When applying general cosmetics, in the afternoon, when compared to the measurement of the phenomenon that the skin moisture rapidly decreases and you can feel dryness, drink a beverage containing 700~1,000 mg of mucopolysaccharide a day as an indicator component as a mucin complex extract continuously for 3 months The degree of whether skin moisture is maintained even in the afternoon and the degree of improvement in skin tone (red/yellow), pores, skin texture, and oil were measured.

Measurement of skin moisture by time when applying general cosmetics

The moisture content of the skin surface was measured using a Corneometer CM 825 PC (Courage-Khazaka Koln, Germany) based on capacitance measurement, a capacitance measurement method, to measure the forehead and cheeks based on the moisture content of the skin epidermis. . The skin condition is expressed in the unit of corneoneter, and the AU (arbitrary units) measurement method displays the value that appears when the probe is placed in close contact with the skin surface of the measurement site and lightly pressed. The average value when measured over time is described. The measurement results are shown in Table 1 below.

Skin test results after ingestion of beverages containing mucin complex extract

The skin tone, pores, texture, and oil content were measured for 3 months using a skin tester, and mucin complex extract type A and type B containing 700-1,000 mg of mucopolysaccharide protein, an indicator component, were ingested. The result is shown in FIG. 5 .

By the mucin extraction method that has undergone ultra-high pressure hydrothermal treatment and fermentation according to the above-described embodiment, the mucin component can be extracted to have a more highly efficient and improved absorption component.

In the above, embodiments of the present invention have been described with reference to the accompanying drawings, but those skilled in the art to which the present invention pertains can be implemented in other specific forms without changing the technical spirit or essential features. You will be able to understand. Therefore, the embodiments described above are illustrative in all respects, and should be understood as non-limiting.

Claims (1)

As a method for preparing a mucin complex extract,
An ultra-high pressure treatment step of hot water extraction of the snails at a predetermined pressure to obtain a snail extract;
First fermentation of the ultra-high pressure treated snails using at least one enzyme of the first group of enzymes; and
Secondary fermentation of the first fermented snail using at least one enzyme of the second group of enzymes,
The enzymes used in the first fermentation step and the second fermentation step are different enzymes,
The ultra-high pressure treatment step, characterized in that proceeds at a temperature of 100 ~ 130 ℃, mucin complex extract manufacturing method.

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