JP5377815B2 - Cosmetic composition for enhancing skin elasticity - Google Patents

Description

  The present invention relates to a cosmetic composition for enhancing skin elasticity, which contains Ginkgo extract, Angelica acutiloba root extract and Yeast extract as active ingredients. More specifically, the present invention contains Ginkgo biloba extract, Toki extract and yeast extract as active ingredients, improves the blood color, prevents fine wrinkles, and prevents the dermis by the combined synergistic action of each extract. -It relates to a cosmetic composition for enhancing skin elasticity, which improves the epidermis and dermis by improving the epidermal boundary layer, thereby improving skin elasticity.

  Skin elasticity is formed by elastic fibers composed of elastin present in the dermis layer. Such elastic fibers have a very low elastic modulus like rubber, and are easily deformed even by a small force, and easily return to the original shape when the force is removed. The elastic fiber has a form in which microfibrils are embedded in an amorphous substrate called elastin. Elastin is a protein composed of very unique amino acids found only in elastic fibers such as desmosine and isodesmosine derived from lysine. Such desmosine, isodesmosine, and the like form cross-links in a long peptide chain. Due to such a structure, elastin has a rubber-like property.

  Elastic fibers composed of elastin exist together with collagen fibers called collagen, and the skin elasticity can be maintained in a state where the elastin and collagen are sufficiently present.

  On the other hand, a decrease in skin elasticity is induced by a decrease in production and destruction of elastin accompanying weakening of skin cells (fibroblasts) due to aging, ultraviolet rays, and the like. That is, when elastin degrading enzyme is expressed in the skin by ultraviolet rays or the like, this enzyme degrades normal elastin in the skin, resulting in a decrease in skin elasticity. In addition, the skin elasticity is reduced due to the damage and reduced production of a protein called collagen that constitutes 70% or more of the skin dermis. Collagen is also degraded by a degrading enzyme called collagenase that is induced by ultraviolet rays or the like.

  On the other hand, as a prior patent related to the increase in skin elasticity, US Pat. No. 5,322,839, which describes that a protein fraction obtained from Leguminosae Seeds shows an effect of increasing elasticity, malt extract Japanese Patent No. 5,105,693, which describes that a composition containing (malt extract) or the like is used for inhibiting collagenase, and as an inhibitor of collagenase or elastase, carbamate peptide (peptidyl) US Pat. No. 5,614, which provides cosmetic compositions containing carbamates, peptidyl thiocarbamates, hydroxamates, cephalosporins, sulphonate salts, and the like. , 489 and the like.

  However, in order to obtain the effect of increasing skin elasticity, the above-mentioned prior patents simply suppress the activity of collagenase or suppress the activity of elastase, and function only in the dermis layer. Has the disadvantage of not being fully demonstrated.

  As a result of studying cosmetic compositions capable of maximizing skin elasticity, the present inventors have found that water-soluble active ingredients such as Ginkgo biloba extract, Toki extract and yeast extract are used as cosmetics. When it was contained in the composition, it was found that the skin elasticity was enhanced by the combination of the respective interactions, and the present invention was completed.

  Accordingly, an object of the present invention is to provide a cosmetic composition for enhancing skin elasticity, which contains a ginkgo biloba extract, a toki extract and a yeast extract as active ingredients.

  In order to achieve the above-mentioned object, the cosmetic composition for enhancing skin elasticity of the present invention comprises, as active ingredients, a ginkgo biloba extract, a Toki extract and a yeast extract, each 0.01% relative to the total weight of the composition. It is contained in an amount of ˜10.0% by weight.

  The cosmetic composition for enhancing skin elasticity of the present invention is a ginkgo biloba extract that has an effect of preventing the degradation of elastin, an extract of Toki that has an effect of preventing the degradation of collagen, and the synthesis of integrin. By containing a yeast extract that has an effect of increasing the amount of the composition, a cosmetic composition having an excellent skin elasticity effect can be provided by the combined synergistic action.

  Hereinafter, the present invention will be described in more detail.

  The ginkgo biloba extract used in the present invention is a ginkgo biloba extract obtained by extracting leaves of GINKGO BILOBA L. using 1,3-butylene glycol, which is a hydrophilic solvent, and water. .

  In general, Ginkgo biloba leaf extract is a total component composed of leaf flavonoids and aromatic oxycarboxylic acid, and has an effect of improving blood flow disturbance in fine arteries and preventing and treating thrombus. In addition, the leaves are used to treat diseases that cause numbness of limbs and blood flow in fine arteries, and have anti-allergic properties as well as antibacterial activity that suppresses Pseudomonas, Diphtheria, Ryukyu, Typhi, Shigella Has effects and the like.

  The citrus extract (ANGELICA ACUTILOBA) used in the present invention is a perennial grass of the dicotyledonous leaflet flower subfamily Umbrella type Apiaceae, and its root is 1,3-butylene glycol, which is a hydrophilic solvent. And an extract extracted with water.

  In general, Toki extract is an important drug material for blood replacement and blood feeding, and is widely known in Chinese medicine. It is effective when the complexion is pale, and improves symptoms such as dizziness and palpitation. In particular, it is effective in treating lack of heart blood and injury, removing blood, and generating new blood. In addition, it acts to suppress E. coli, Salmonella typhi, etc., antagonizes vitamin E deficiency, intestines and stool, protects the liver and prevents the reduction of glycogen.

  The yeast extract used in the present invention is obtained by producing and extracting yeast by microbial fermentation. In general, yeast extract has a function of promoting the synthesis of collagen IV, collagen VII and integrin.

  In the cosmetic composition for enhancing skin elasticity of the present invention, Ginkgo biloba extract, Toki extract and yeast extract are used as active ingredients in amounts of 0.01 to 10.0% by weight, respectively, based on the total weight of the composition. contains.

  The dosage form of the cosmetic composition for enhancing skin elasticity of the present invention is not particularly limited. Specifically, for example, it is produced as a dosage form such as soft lotion, nutritional lotion, nutritional cream, massage cream, essence and the like. can do.

  EXAMPLES Hereinafter, although this invention is demonstrated in detail by a test example and an Example, this invention is not limited only to these examples.

[Test Example 1] Elastin production promoting effect of Ginkgo biloba extract It was collected directly from humans, or commercially available human fibroblasts were treated with Ginkgo biloba extract, and the inhibitory effect on elastase activity was measured. The specific experimental method is as follows.

  As the Ginkgo biloba extract used in the experiment, the Ginkgo biloba extract whose trade name is Ginko Extract (product of GINKO EXTRACT, BIOLAND Co.) was used.

First, human fibroblasts are placed in a culture flask and cultured until they grow to about 70 to 80%, and then the ginkgo biloba extract is treated for 1 day, and the cell culture solution is collected to obtain a commercially available elastin measuring instrument. Was used to measure the production of elastin (Biochemical Medicine 11: 350-357, 1974 “The synthesis and analytical use of a highly sensitive and covenient substrate of elastase” and J. Invest Dermatol 86: 63-68, 1986 “Elastase and neutral cathepsin production by human fibroblasts effect of culture conditions on synthesis and secretion ”). That is, the activity of elastase was measured by measuring the color change caused by NA degradation using Suc- (Ala) 3NA, which is a substrate for elastase, using absorbance. At this time, a group not treated with Ginkgo biloba extract was used as a control group. The experimental results are shown in Table 1 below.

[Test Example 2] Collagen production promoting effect of Toki extract A human fibroblast was collected directly from humans or treated with Toki extract to measure the inhibitory effect of collagenase activity. The specific experimental method is as follows.

  As the Toki extract used in the experiment, a Toki extract whose trade name is Toki extract (product of ANGELICA ACUTILOBA ROOT EXTRACT, Green Tech.) Was used.

First, human fibroblasts are placed in a culture flask and cultured until it grows to about 70 to 80%, then treated with a Japanese extract of cinnamon for 1 day, a cell culture solution is collected, and a commercially available collagenase measuring instrument is used. The production of collagen was measured (see Anal Biochem 116 (1): 172-7, 1987 “An assay of collagenase activity using enzyme-linked immunosorbent assay for mammalian collagenase”). That is, collagenase activity was measured by quantifying human-derived MMP-1 (Matrix Metalloproteinase-1) using an antibody against anti-collagenase. At this time, a group not treated with Toki extract was used as a control group. The experimental results are shown in Table 2 below.

[Test Example 3] Elastin production promoting effect of yeast extract The elastin production effect was measured by collecting human fibroblasts directly from humans or treating human fibroblasts with yeast extract. The experimental method is as follows.

  As the yeast extract used in the experiment, a yeast extract having a trade name of Yeast Extract or Toniskin (product of YEAST EXTRACT or TONISKIN, Silab Co.) was used.

First, human fibroblasts are put in a culture flask and cultured until it grows to about 70 to 80%, then the yeast extract is treated for 1 day, a cell culture solution is collected, and a commercially available elastin measuring instrument is used. The elastin production rate was measured using this. At this time, a group not treated with the yeast extract was used as a control group. The experimental results are shown in Table 3 below.

<Example 1 and Comparative Examples 1-7>
Based on the composition of Table 4 below, cosmetic compositions of Example 1 and Comparative Examples 1 to 7 were produced in the form of a lotion.

Production Method (1) Components 1 to 3 (A) and Components 4 to 8 (B) are each heated to 75 ° C.

  (2) While adding the A to the B little by little, emulsify and stir at a speed of 7000 rpm. Next, after all the A is added, the mixture is emulsified and stirred for 2 minutes.

  (3) Next, it stirs at 2500 rpm for 1 minute, and deaerates air from a composition.

  (4) The beaker containing the composition is immersed in ice water and cooled to 28-30 ° C.

  (5) The obtained composition is allowed to stand at room temperature for 24 hours to be stabilized.

[Test Example 4]
After irradiating hairless mice with ultraviolet rays once a day for 8 weeks (UVB 70 mJ / cm ² ), it was divided into two groups (20 mice per group), and Comparative Examples 1 to 7 and Example 1 were After applying 100 ml / animal on the back daily for one month, the skin was collected and the formation of elastin and collagen was measured histologically. In the measurement, after elastin and collagen were stained, the degree of increase in elastin and collagen was determined by image analysis of the degree of staining. The experimental results are shown in Table 5 below.

  As is clear from the results of Table 5, Comparative Example 2 containing only Ginkgo biloba extract as an active ingredient and Example 1 containing Ginkgo biloba extract, Toki extract and yeast extract as active ingredients. By comparison, it can be seen that Example 1 shows a significant increase in elastin by the Toki extract and the yeast extract.

  In addition, if Comparative Example 3 containing only a Toki extract as an active ingredient is compared with Example 1 containing a Toki extract, a Ginkgo biloba extract and a yeast extract as an active ingredient, Example 1 would be Ginkgo biloba. It can be seen that the extract and yeast extract show a significant increase in collagen.

[Test Example 5] Moisturizing power and skin elasticity improvement degree (clinical test)
In order to compare the skin elasticity recovery effect with the lotions of Example 1 and Comparative Examples 1 to 7 produced above, 280 women in their 30s and 40s were targeted, and 20 people as a group Measurements were divided into groups. The experimental method is as follows.

  Example 1 and Comparative Examples 1-7 were provided to each group and applied once a day for one month. One month later, the elasticity of the facial region was measured using a cutometer (SEM474, Courage + Khazaka electronic GmbH. Germany) capable of measuring skin elasticity, and compared with before application. Moreover, the moisture retention power was measured using a corneometer (CM820 Courage + Khazaka electronic GmbH. Germany).

The experimental results are shown in Table 6 below as average values for each group.

  As is clear from the results of Table 6 above, compared to the case where only the ginkgo biloba extract is contained as an active ingredient (Comparative Example 2) or the case where only the Toki extract is contained as an active ingredient (Comparative Example 3), In addition to these components, it can be seen that the case of further containing yeast extract as an active ingredient (Example 1) shows a remarkable synergistic effect in both moisturizing power and improvement in skin elasticity.

  As mentioned above, the mixture of Ginkgo leaf extract (Ginkgo extract), Toki extract (Angelic a acutiloba root extract) and yeast extract (Yeast extract) is useful as a skin elasticity enhancer, and the composition containing these is It turns out that it is useful as a cosmetic composition for enhancing skin elasticity.

<Example 2> Suspension emulsion lotion A suspension emulsion lotion of Example 2 was produced based on the composition shown in Table 7 below.

<Example 3> Essence The essence of Example 3 was produced based on the composition shown in Table 8 below.

<Example 4> Nutritional cream The nutritional cream of Example 4 was manufactured based on the composition of Table 9 below.

Example 5 Gel A gel of Example 5 was produced based on the composition shown in Table 10 below.

<Example 6> Pack A pack of Example 6 was manufactured based on the composition shown in Table 11 below.

Claims (2)

  A cosmetic composition for enhancing skin elasticity, comprising a ginkgo biloba extract, a toki extract, and a yeast extract as active ingredients.   The cosmetic composition for enhancing skin elasticity according to claim 1, wherein the active ingredient is contained in an amount of 0.01 to 10.0% by weight based on the total weight of the composition.