JP5329978B2 - メラノーマの自己破壊を引き起こすナノ秒パルス電界 - Google Patents

メラノーマの自己破壊を引き起こすナノ秒パルス電界 Download PDF

Info

Publication number
JP5329978B2
JP5329978B2 JP2008556458A JP2008556458A JP5329978B2 JP 5329978 B2 JP5329978 B2 JP 5329978B2 JP 2008556458 A JP2008556458 A JP 2008556458A JP 2008556458 A JP2008556458 A JP 2008556458A JP 5329978 B2 JP5329978 B2 JP 5329978B2
Authority
JP
Japan
Prior art keywords
nspef
electric field
tumor
pulse
melanoma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2008556458A
Other languages
English (en)
Japanese (ja)
Other versions
JP2009532077A (ja
JP2009532077A5 (cg-RX-API-DMAC10.html
Inventor
リチャード ヌッチテッリ
シュテファン ジェー． ビービ
カール エイチ． シェーンバッハ
Original Assignee
イースタン バージニア メディカル スクール
オールド ドミニオン ユニバーシティー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イースタン バージニア メディカル スクール, オールド ドミニオン ユニバーシティー filed Critical イースタン バージニア メディカル スクール
Publication of JP2009532077A publication Critical patent/JP2009532077A/ja
Publication of JP2009532077A5 publication Critical patent/JP2009532077A5/ja
Application granted granted Critical
Publication of JP5329978B2 publication Critical patent/JP5329978B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/20—Applying electric currents by contact electrodes continuous direct currents
    • A61N1/205—Applying electric currents by contact electrodes continuous direct currents for promoting a biological process
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/36002—Cancer treatment, e.g. tumour

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Radiology & Medical Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Electrotherapy Devices (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2008556458A 2006-02-24 2007-02-26 メラノーマの自己破壊を引き起こすナノ秒パルス電界 Active JP5329978B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US77621506P 2006-02-24 2006-02-24
US60/776,215 2006-02-24
PCT/US2007/004844 WO2007100727A2 (en) 2006-02-24 2007-02-26 Nanosecond pulsed electric fields cause melanomas to self-destruct

Publications (3)

Publication Number Publication Date
JP2009532077A JP2009532077A (ja) 2009-09-10
JP2009532077A5 JP2009532077A5 (cg-RX-API-DMAC10.html) 2010-04-08
JP5329978B2 true JP5329978B2 (ja) 2013-10-30

Family

ID=38459596

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008556458A Active JP5329978B2 (ja) 2006-02-24 2007-02-26 メラノーマの自己破壊を引き起こすナノ秒パルス電界

Country Status (5)

Country Link
US (3) US9168373B2 (cg-RX-API-DMAC10.html)
JP (1) JP5329978B2 (cg-RX-API-DMAC10.html)
AU (1) AU2007221182B2 (cg-RX-API-DMAC10.html)
CA (1) CA2643210C (cg-RX-API-DMAC10.html)
WO (1) WO2007100727A2 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7748327B2 (ja) 2016-11-09 2025-10-02 パルス バイオサイエンシズ インコーポレイテッド 低電圧インピーダンスチェックパルス発生器

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994706B2 (en) 2001-08-13 2006-02-07 Minnesota Medical Physics, Llc Apparatus and method for treatment of benign prostatic hyperplasia
US12697172B2 (en) 2008-04-29 2026-08-04 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US9283051B2 (en) 2008-04-29 2016-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating a treatment volume for administering electrical-energy based therapies
US9198733B2 (en) 2008-04-29 2015-12-01 Virginia Tech Intellectual Properties, Inc. Treatment planning for electroporation-based therapies
US10272178B2 (en) 2008-04-29 2019-04-30 Virginia Tech Intellectual Properties Inc. Methods for blood-brain barrier disruption using electrical energy
US10238447B2 (en) 2008-04-29 2019-03-26 Virginia Tech Intellectual Properties, Inc. System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress
US8992517B2 (en) 2008-04-29 2015-03-31 Virginia Tech Intellectual Properties Inc. Irreversible electroporation to treat aberrant cell masses
US11254926B2 (en) 2008-04-29 2022-02-22 Virginia Tech Intellectual Properties, Inc. Devices and methods for high frequency electroporation
US10702326B2 (en) 2011-07-15 2020-07-07 Virginia Tech Intellectual Properties, Inc. Device and method for electroporation based treatment of stenosis of a tubular body part
US10245098B2 (en) 2008-04-29 2019-04-02 Virginia Tech Intellectual Properties, Inc. Acute blood-brain barrier disruption using electrical energy based therapy
US10448989B2 (en) 2009-04-09 2019-10-22 Virginia Tech Intellectual Properties, Inc. High-frequency electroporation for cancer therapy
US11272979B2 (en) 2008-04-29 2022-03-15 Virginia Tech Intellectual Properties, Inc. System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies
EP2280741A4 (en) 2008-04-29 2012-06-13 Virginia Tech Intell Prop IRREVERSIBLE ELECTROPORATION FOR THE PRODUCTION OF TISSUE OBJECTS
US9867652B2 (en) 2008-04-29 2018-01-16 Virginia Tech Intellectual Properties, Inc. Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds
WO2010107947A1 (en) * 2009-03-17 2010-09-23 Bioelectromed Corp. Nanosecond pulsed electric field parameters for destroying tumors with a single treatment
US8632534B2 (en) 2009-04-03 2014-01-21 Angiodynamics, Inc. Irreversible electroporation (IRE) for congestive obstructive pulmonary disease (COPD)
US11638603B2 (en) 2009-04-09 2023-05-02 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US11382681B2 (en) 2009-04-09 2022-07-12 Virginia Tech Intellectual Properties, Inc. Device and methods for delivery of high frequency electrical pulses for non-thermal ablation
US8903488B2 (en) 2009-05-28 2014-12-02 Angiodynamics, Inc. System and method for synchronizing energy delivery to the cardiac rhythm
US9895189B2 (en) 2009-06-19 2018-02-20 Angiodynamics, Inc. Methods of sterilization and treating infection using irreversible electroporation
WO2012051433A2 (en) 2010-10-13 2012-04-19 Angiodynamics, Inc. System and method for electrically ablating tissue of a patient
JP5854494B2 (ja) * 2011-03-31 2016-02-09 国立大学法人 熊本大学 細胞改変方法
US9078665B2 (en) 2011-09-28 2015-07-14 Angiodynamics, Inc. Multiple treatment zone ablation probe
US9414881B2 (en) 2012-02-08 2016-08-16 Angiodynamics, Inc. System and method for increasing a target zone for electrical ablation
WO2014105964A1 (en) * 2012-12-27 2014-07-03 The General Hospital Corporation Systems and methods for delivering pulsed electric fields to skin tissue
CA2914195C (en) 2013-06-03 2020-06-02 Nanoblate Corporation Methods and devices for stimulating an immune response using nanosecond pulsed electric fields
EP3113605B1 (en) 2014-03-04 2018-10-31 Pulse Biosciences, Inc. Marking tissue surfaces for treatment of lesions by electrical nanopulses
EP3143124B1 (en) 2014-05-12 2025-01-22 Virginia Tech Intellectual Properties, Inc. Selective modulation of intracellular effects of cells using pulsed electric fields
US12114911B2 (en) 2014-08-28 2024-10-15 Angiodynamics, Inc. System and method for ablating a tissue site by electroporation with real-time pulse monitoring
US9724155B2 (en) 2014-12-01 2017-08-08 Pulse Biosciences, Inc. Nanoelectroablation control and vaccination
WO2016100325A1 (en) 2014-12-15 2016-06-23 Virginia Tech Intellectual Properties, Inc. Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment
US10874451B2 (en) 2016-02-29 2020-12-29 Pulse Biosciences, Inc. High-voltage analog circuit pulser and pulse generator discharge circuit
US10548665B2 (en) 2016-02-29 2020-02-04 Pulse Biosciences, Inc. High-voltage analog circuit pulser with feedback control
WO2017165502A1 (en) * 2016-03-22 2017-09-28 The General Hospital Corporation Compositions and methods for topical delivery of pharmaceutical agents
DK3474760T3 (da) 2016-06-27 2023-03-20 Galvanize Therapeutics Inc Generator og et kateter med en elektrode til at behandle en lungepassage
US12403305B2 (en) 2016-06-27 2025-09-02 Galvanize Therapeutics, Inc. Immunostimulation in the treatment of viral infection
US10543357B2 (en) 2016-09-19 2020-01-28 Pulse Biosciences, Inc. High voltage connectors for pulse generators
WO2018075946A1 (en) 2016-10-20 2018-04-26 Old Dominion University Research Foundation Methods and devices for using sub-microsecond electric pulses to trigger apoptosis
US10905492B2 (en) 2016-11-17 2021-02-02 Angiodynamics, Inc. Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode
WO2018106672A1 (en) * 2016-12-05 2018-06-14 Old Dominion University Research Foundation Methods and devices for treatment of tumors with nano-pulse stimulation
JP2018094078A (ja) * 2016-12-13 2018-06-21 之一 市川 高電界を利用した皮下脂肪減少装置
US10946193B2 (en) 2017-02-28 2021-03-16 Pulse Biosciences, Inc. Pulse generator with independent panel triggering
US10850095B2 (en) * 2017-08-08 2020-12-01 Pulse Biosciences, Inc. Treatment of tissue by the application of energy
US11590345B2 (en) 2017-08-08 2023-02-28 Pulse Biosciences, Inc. Treatment of tissue by the application of energy
US10857347B2 (en) 2017-09-19 2020-12-08 Pulse Biosciences, Inc. Treatment instrument and high-voltage connectors for robotic surgical system
US11607537B2 (en) 2017-12-05 2023-03-21 Virginia Tech Intellectual Properties, Inc. Method for treating neurological disorders, including tumors, with electroporation
EP4552592A3 (en) 2017-12-26 2025-06-18 Galvanize Therapeutics, Inc. Optimization of energy delivery for various applications
US12390262B2 (en) 2018-03-13 2025-08-19 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US11311329B2 (en) * 2018-03-13 2022-04-26 Virginia Tech Intellectual Properties, Inc. Treatment planning for immunotherapy based treatments using non-thermal ablation techniques
US11925405B2 (en) 2018-03-13 2024-03-12 Virginia Tech Intellectual Properties, Inc. Treatment planning system for immunotherapy enhancement via non-thermal ablation
US11577071B2 (en) 2018-03-13 2023-02-14 Pulse Biosciences, Inc. Moving electrodes for the application of electrical therapy within a tissue
CN110819619A (zh) * 2018-08-10 2020-02-21 北京大学 纳秒脉冲电场在提高细胞干性中的用途
WO2020077019A1 (en) 2018-10-10 2020-04-16 Pulse Biosciences, Inc. Treatment of dermal glands by the application of non-thermal energy
JP7146310B2 (ja) 2018-11-08 2022-10-04 オールド ドミニオン ユニバーシティ リサーチ ファウンデーション ナノ秒パルスバーストのメガヘルツ圧縮
US11043745B2 (en) 2019-02-11 2021-06-22 Old Dominion University Research Foundation Resistively loaded dielectric biconical antennas for non-invasive treatment
US11571569B2 (en) 2019-02-15 2023-02-07 Pulse Biosciences, Inc. High-voltage catheters for sub-microsecond pulsing
WO2020191301A1 (en) 2019-03-21 2020-09-24 Pulse Biosciences, Inc. Tattoo removal
CN114007535A (zh) 2019-04-18 2022-02-01 盖乐世公司 用于治疗异常组织的装置、系统和方法
US11950835B2 (en) 2019-06-28 2024-04-09 Virginia Tech Intellectual Properties, Inc. Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy
CA3147592A1 (en) 2019-07-16 2021-01-21 Galary, Inc. Treatment of the reproductive tract with pulsed electric fields
US12214189B2 (en) 2019-07-24 2025-02-04 Virginia Tech Intellectual Properties, Inc. Fourier analysis spectroscopy for monitoring tissue impedance changes and treatment outcome during electroporation-based-therapies
JP2022547634A (ja) 2019-10-01 2022-11-14 パルス バイオサイエンシズ インコーポレイテッド ナノ秒パルス電源における負荷電流を制限するための装置および方法
US11452870B2 (en) 2019-12-18 2022-09-27 Pulse Biosciences, Inc. Nanosecond pulsed power sources having multi-core transformers
CN115348844A (zh) 2020-04-08 2022-11-15 盖能适治疗股份有限公司 脉冲电场波形操作和使用
US11904164B2 (en) 2020-07-27 2024-02-20 Pulse Biosciences, Inc. Nanosecond pulsed electric field system
EP4208114B1 (en) 2020-09-02 2025-03-26 Pulse Biosciences, Inc. Universal handpiece for electrical treatment applicator
US12485279B2 (en) 2020-11-25 2025-12-02 Virginia Tech Intellectual Properties, Inc. Methods for modulating temporal infrastructure of pulsed electric fields
JP7749695B2 (ja) 2021-04-26 2025-10-06 パルス バイオサイエンシズ インコーポレイテッド 周方向アブレーションデバイス及び方法
US12446944B2 (en) 2021-04-26 2025-10-21 Pulse Biosciences, Inc. Mapping and ablation applicators
US12564440B2 (en) 2021-04-26 2026-03-03 Pulse Biosciences, Inc. Multi-strut ablation and sensing catheter devices and methods
US12667717B2 (en) 2021-08-10 2026-06-30 Pulse Biosciences, Inc. Electrical applicators for applying energy to tissue surfaces or regions superficial to the surface
US12042653B2 (en) 2021-12-30 2024-07-23 Pulse Biosciences, Inc. Electrical applicators with non-penetrating electrodes for applying energy to tissue surfaces

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6326177B1 (en) * 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
US20020010491A1 (en) * 1999-08-04 2002-01-24 Schoenbach Karl H. Method and apparatus for intracellular electro-manipulation
US7653438B2 (en) * 2002-04-08 2010-01-26 Ardian, Inc. Methods and apparatus for renal neuromodulation
WO2005032646A2 (en) * 2003-07-18 2005-04-14 Eastern Virginia Medical School Apparatus for generating electrical pulses and methods of using the same
US20050171574A1 (en) * 2003-12-24 2005-08-04 The Regents Of The University Of California Electroporation to interrupt blood flow
CN101553180B (zh) * 2006-09-14 2013-08-07 拉热尔技术有限公司 用于破坏癌细胞的装置
US7655004B2 (en) * 2007-02-15 2010-02-02 Ethicon Endo-Surgery, Inc. Electroporation ablation apparatus, system, and method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7748327B2 (ja) 2016-11-09 2025-10-02 パルス バイオサイエンシズ インコーポレイテッド 低電圧インピーダンスチェックパルス発生器

Also Published As

Publication number Publication date
US10905874B2 (en) 2021-02-02
WO2007100727A2 (en) 2007-09-07
WO2007100727A3 (en) 2008-02-21
AU2007221182B2 (en) 2011-11-03
US20160015971A1 (en) 2016-01-21
US20180200510A1 (en) 2018-07-19
AU2007221182A1 (en) 2007-09-07
JP2009532077A (ja) 2009-09-10
CA2643210C (en) 2018-05-01
US20110092973A1 (en) 2011-04-21
AU2007221182A2 (en) 2008-10-02
CA2643210A1 (en) 2007-09-07
US9168373B2 (en) 2015-10-27
US9943684B2 (en) 2018-04-17

Similar Documents

Publication Publication Date Title
JP2009532077A (ja) メラノーマの自己破壊を引き起こすナノ秒パルス電界
Nuccitelli et al. Nanosecond pulsed electric fields cause melanomas to self-destruct
US20220280228A1 (en) Enhanced needle array and therapies for tumor ablation
Nuccitelli et al. Nanoelectroablation of human pancreatic carcinoma in a murine xenograft model without recurrence
US20250195128A1 (en) Methods, systems, and apparatuses for tissue ablation using a modulated exponential decay pulse
Chen et al. Long term survival of mice with hepatocellular carcinoma after pulse power ablation with nanosecond pulsed electric fields
Nuccitelli et al. Optimized nanosecond pulsed electric field therapy can cause murine malignant melanomas to self‐destruct with a single treatment
Nuccitelli et al. Non‐thermal nanoelectroablation of UV‐induced murine melanomas stimulates an immune response
JP6723153B2 (ja) 生体内の望ましくない軟部組織を切除するシステム
US6972013B1 (en) Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
US8926606B2 (en) Integration of very short electric pulses for minimally to noninvasive electroporation
JP5503575B2 (ja) 細胞内エレクトロマニピュレーション方法
US20120109122A1 (en) High-frequency electroporation for cancer therapy
US8682426B2 (en) Delivery device, system, and method for delivering nanosecond pulsed electric fields
WO2000047274A1 (en) Iontophoresis, electroporation and combination patches for local drug delivery
CN112292090A (zh) 用于治疗疾病或不需要的组织的电热疗法
Granata et al. Electroporation-based treatments in minimally invasive percutaneous, laparoscopy and endoscopy procedures for treatment of deep-seated tumors.
Sheehan et al. Pulsed electric fields
Nie et al. The state-of-the-art of atmospheric pressure plasma for transdermal drug delivery
Luo et al. Nanosecond pulsed bipolar cancellation of the killing effect on glioblastoma
Kurata et al. Feasibility of concentric electrodes in contact irreversible electroporation for superficial lesion treatment
Nuccitelli et al. Nanosecond pulsed electric fields cause melanomas to self-destruct. PEMF and skin Cancer. 12 May Nanosecond pulsed electric fields cause melanomas to self-destruct. PEMF and skin Cancer.
Cabuy Pulsed electric fields in cancer treatment
Sheehan¹ et al. Pulsed electric fields
HK40031252A (en) Cancer immunotherapy by radiofrequency electrical membrane breakdown (rf-emb)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100217

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20100217

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20120309

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120314

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120613

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20121105

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130204

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130626

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130725

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

Ref document number: 5329978

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313117

S531 Written request for registration of change of domicile

Free format text: JAPANESE INTERMEDIATE CODE: R313531

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250