JP5325793B2 - リグニンを除去するために、アルカリ溶液で固体リグノセルロース材料を処理するステップを含む、固体リグノセルロース材料からのエタノールの発酵生産のための方法 - Google Patents
リグニンを除去するために、アルカリ溶液で固体リグノセルロース材料を処理するステップを含む、固体リグノセルロース材料からのエタノールの発酵生産のための方法 Download PDFInfo
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- JP5325793B2 JP5325793B2 JP2009538790A JP2009538790A JP5325793B2 JP 5325793 B2 JP5325793 B2 JP 5325793B2 JP 2009538790 A JP2009538790 A JP 2009538790A JP 2009538790 A JP2009538790 A JP 2009538790A JP 5325793 B2 JP5325793 B2 JP 5325793B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Description
a)アルカリ培地中でのセルロリグニンの前処理及びそれに続く水を用いた洗浄;
b)市販の酵素が装填された状態での、中程度の温度での、好ましくは8〜12時間の期間の、残渣の酵素的加水分解(糖化)のための生物反応器への残渣の移動;
c)エタノールの生産に適合した酵母の添加及び最大期間32時間の酵素濃度の維持による、前のステップにおいて加水分解された材料のセルロース画分の同時の発酵;
d)生成したエタノールの分離及び蒸留。
(実施例1)
(実施例2)
Claims (12)
- リグノセルロース材料からエタノールを酵素的に生産する方法であって、
a)セルロース及びリグニンを含む固体物を、アルカリ溶液による1回又は複数回の洗浄にかけて、前記固体物からリグニンを少なくとも部分的に除去し、酸性化した水で洗浄して、6〜7のpHとし、水相から残りの前記固体物を分離するステップ;次いで
b)固体物を、まだ前記反応器中で、加水分解するステップであって、固体物を、微量栄養素の溶液及びセルロース分解酵素と反応させることを含み、ここで、固体:液体比は1:10〜1:20であり、温度は30℃〜50℃であり、酵素濃度は20〜30FPU/gであり、反応は8〜12時間進行させるステップ;並びに
c)酵素的加水分解に由来する培地を同時発酵によって発酵するステップであって、エタノール生産酵母を追加した後の培地中の追加されたエタノール生産酵母の細胞濃度が2〜6g/Lであるエタノール生産酵母を追加すること、及び32時間以内、温度を30℃〜39℃に維持することを含むステップ;次いで
d)蒸留によってエタノールを分離するステップ
を含む、上記方法。 - 酵母は、非遺伝子改変サッカロミセス セレビシエ(Saccharomyces cerevisiae)酵母である請求項1に記載の方法。
- 方法の生産率は1.0〜3.0g/L.hの範囲である請求項1又は2に記載の方法。
- 方法の全期間は32時間以内である請求項1から3までのいずれか一項に記載の方法。
- 加水分解は45℃〜49℃の温度で進められる請求項1から4までのいずれか一項に記載の方法。
- 発酵は35℃〜39℃の温度で進められる請求項1から5までのいずれか一項に記載の方法。
- ステップc)の酵母のバイオマスの濃度は2〜6g/Lの範囲で維持される請求項1から6までのいずれか一項に記載の方法。
- ステップb)に記載される酵素は市販のセルロース酵素調製物を加えることにより提供される請求項1から7までのいずれか一項に記載の方法。
- リグノセルロース材料はサトウキビバガスを含む請求項1から8までのいずれか一項に記載の方法。
- 同時糖化発酵(SSF)がステップc)において実施される請求項1に記載の方法。
- リグノセルロース材料からエタノールを酵素的に生産する方法であって、
i)リグノセルロース材料を加水分解し、及び発酵させ、並びに固体物を保持するステップ;並びに
ii)前記固体物に対して、請求項1から10までのいずれか一項に記載の方法を実施するステップを含む、上記方法。 - リグノセルロース材料はサトウキビバガスを含む請求項11に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BRPI0605017-4 | 2006-11-30 | ||
BRPI0605017-4A BRPI0605017B1 (pt) | 2006-11-30 | 2006-11-30 | processo para a produção de etanol a partir de materiais lignocelulósicos por via enzimática |
PCT/GB2007/004618 WO2008065433A1 (en) | 2006-11-30 | 2007-11-30 | Process for the fermentative production of ethanol from solid lignocellulosic material comprising a step of treating a solid lignocellulosic material with alkaline solution in order to remove the lignin |
Publications (3)
Publication Number | Publication Date |
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JP2010510800A JP2010510800A (ja) | 2010-04-08 |
JP2010510800A5 JP2010510800A5 (ja) | 2010-11-11 |
JP5325793B2 true JP5325793B2 (ja) | 2013-10-23 |
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JP2009538790A Active JP5325793B2 (ja) | 2006-11-30 | 2007-11-30 | リグニンを除去するために、アルカリ溶液で固体リグノセルロース材料を処理するステップを含む、固体リグノセルロース材料からのエタノールの発酵生産のための方法 |
Country Status (11)
Country | Link |
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US (1) | US8232082B2 (ja) |
JP (1) | JP5325793B2 (ja) |
CN (1) | CN101426922A (ja) |
AR (1) | AR062267A1 (ja) |
BR (1) | BRPI0605017B1 (ja) |
CA (1) | CA2660673C (ja) |
CO (1) | CO5880297A1 (ja) |
DK (1) | DK178525B1 (ja) |
GB (1) | GB2454119B (ja) |
SE (1) | SE535902C2 (ja) |
WO (1) | WO2008065433A1 (ja) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
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US9399782B2 (en) | 2007-06-27 | 2016-07-26 | Novozymes A/S | Methods for producing fermentation products |
JP5233452B2 (ja) * | 2008-07-08 | 2013-07-10 | 王子ホールディングス株式会社 | 糖化発酵システム |
CA2739704C (en) | 2008-11-20 | 2015-08-18 | E.I. Du Pont De Nemours And Company | Process for producing a sugar solution by combined chemical and enzymatic saccharification of polysaccharide enriched biomass |
WO2010059825A1 (en) * | 2008-11-20 | 2010-05-27 | E. I. Du Pont De Nemours And Company | Sugar production by decrystallization and hydrolysis of polysaccharide enriched biomass |
US8524474B2 (en) * | 2008-11-20 | 2013-09-03 | E I Du Pont De Nemours And Company | Process for producing a concentrated sugar solution by enzymatic saccharification of polysaccharide enriched biomass |
RU2405832C2 (ru) | 2008-12-29 | 2010-12-10 | Дэвон Инвестмент Лимитед | Способ подготовки древесного сырья для получения сахаров, установка для его осуществления и продукт |
BRPI0805560B1 (pt) | 2008-12-29 | 2016-12-06 | Petroleo Brasileiro Sa | processo de produção de um preparado enzimático para hidrólise de celulose de resíduos lignocelulósicos e sua aplicação na produção de etanol |
MY171749A (en) | 2009-05-26 | 2019-10-27 | Arvind Mallinath Lali | Method for production of fermentable sugars from biomass |
WO2011031104A2 (ko) | 2009-09-14 | 2011-03-17 | 지에스칼텍스 주식회사 | 바이오 연료물질 및 바이오 화학물질 제조방법 |
BR122016004370B1 (pt) * | 2009-10-08 | 2018-01-16 | Dsm Ip Assets B.V. | Processo para a preparação de etanol de material lignocelulósico |
JP2012044880A (ja) * | 2010-07-29 | 2012-03-08 | Sekisui Chem Co Ltd | セルロース糖化方法 |
US8889384B2 (en) | 2010-10-07 | 2014-11-18 | Shell Oil Company | Process for the production of alcohols from biomass |
FR2966154B1 (fr) | 2010-10-13 | 2014-05-16 | Cie Ind De La Matiere Vegetale Cimv | Procede perfectionne d'hydrolyse enzymatique de la cellulose |
CN102051383A (zh) * | 2010-11-05 | 2011-05-11 | 中国科学院过程工程研究所 | 汽爆处理木质纤维素原料联产沼气、纤维素和木质素的方法 |
US8609379B2 (en) | 2010-12-20 | 2013-12-17 | Shell Oil Company | Process for the production of alcohols from biomass |
CN102251010B (zh) * | 2011-05-18 | 2014-12-24 | 广州优锐生物科技有限公司 | 一种蔗渣高效同步糖化共发酵产乙醇的方法 |
WO2013095204A1 (en) * | 2011-12-21 | 2013-06-27 | Skandinavisk Kemiinformation Ab | Method for preparing fuels from lignocellulosic feedstock |
CN117025713B (zh) * | 2023-09-21 | 2024-02-13 | 北京工商大学 | 一种复合菌系制备乙醇的方法 |
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US5628830A (en) | 1979-03-23 | 1997-05-13 | The Regents Of The University Of California | Enzymatic hydrolysis of biomass material |
AU1584201A (en) | 1999-11-02 | 2001-05-14 | Waste Energy Integrated Sytems, Llc | Process for the production of organic products from lignocellulose containing biomass sources |
US7604967B2 (en) * | 2003-03-19 | 2009-10-20 | The Trustees Of Dartmouth College | Lignin-blocking treatment of biomass and uses thereof |
JP4401735B2 (ja) * | 2003-10-15 | 2010-01-20 | 月島機械株式会社 | エタノールの製造方法 |
US8003352B2 (en) * | 2004-07-16 | 2011-08-23 | Iogen Energy Corporation | Method of obtaining a product sugar stream from cellulosic biomass |
BRPI0505299B1 (pt) | 2005-11-11 | 2014-12-16 | Petroleo Brasileiro Sa | Processo de produção de etanol a partir do hidrolisado da fração hemicelulósica do bagaço de cana-de-açúcar em reator do tipo prensa |
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WO2008065433A1 (en) | 2008-06-05 |
DK200900242A (en) | 2009-02-23 |
SE535902C2 (sv) | 2013-02-12 |
GB0901590D0 (en) | 2009-03-11 |
CO5880297A1 (es) | 2008-01-31 |
CA2660673A1 (en) | 2008-06-05 |
AR062267A1 (es) | 2008-10-29 |
BRPI0605017B1 (pt) | 2020-11-03 |
GB2454119B (en) | 2011-10-19 |
US8232082B2 (en) | 2012-07-31 |
SE0950098L (sv) | 2009-02-23 |
US20100035318A1 (en) | 2010-02-11 |
DK178525B1 (en) | 2016-05-23 |
CN101426922A (zh) | 2009-05-06 |
GB2454119A (en) | 2009-04-29 |
JP2010510800A (ja) | 2010-04-08 |
BRPI0605017A (pt) | 2008-07-15 |
CA2660673C (en) | 2014-12-30 |
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