JP5325448B2 - 固相を用いての核酸分子の単離および精製 - Google Patents

固相を用いての核酸分子の単離および精製 Download PDF

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JP5325448B2
JP5325448B2 JP2008107526A JP2008107526A JP5325448B2 JP 5325448 B2 JP5325448 B2 JP 5325448B2 JP 2008107526 A JP2008107526 A JP 2008107526A JP 2008107526 A JP2008107526 A JP 2008107526A JP 5325448 B2 JP5325448 B2 JP 5325448B2
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solid phase
nucleic acid
nucleic acids
dna
adsorption
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JP2008263982A (ja
JP2008263982A5 (enExample
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ビルクナー クリスティアン
フォン デア エルツ ヘルベルト
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エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2008107526A 2007-04-20 2008-04-17 固相を用いての核酸分子の単離および精製 Active JP5325448B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008073 2007-04-20
EP07008073.4 2007-04-20

Related Child Applications (1)

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JP2012178156A Division JP5685227B2 (ja) 2007-04-20 2012-08-10 固相を用いての核酸分子の単離および精製

Publications (3)

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JP2008263982A JP2008263982A (ja) 2008-11-06
JP2008263982A5 JP2008263982A5 (enExample) 2010-05-13
JP5325448B2 true JP5325448B2 (ja) 2013-10-23

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JP2008107526A Active JP5325448B2 (ja) 2007-04-20 2008-04-17 固相を用いての核酸分子の単離および精製
JP2012178156A Active JP5685227B2 (ja) 2007-04-20 2012-08-10 固相を用いての核酸分子の単離および精製

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JP2012178156A Active JP5685227B2 (ja) 2007-04-20 2012-08-10 固相を用いての核酸分子の単離および精製

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US (1) US8101744B2 (enExample)
EP (1) EP1983051B1 (enExample)
JP (2) JP5325448B2 (enExample)
CN (1) CN101289661B (enExample)
CA (1) CA2629589C (enExample)

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ATE551433T1 (de) * 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
EP2288726B1 (en) 2008-05-13 2012-06-13 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
US20120141980A1 (en) * 2009-08-17 2012-06-07 Merk Patent Gesellschaft Mit Beschrankter Haftung Method for isolating viruses
US9371509B2 (en) 2009-09-02 2016-06-21 Roche Diagnostics Operations, Inc. Reagents for lysis of bacterial cells
US8420801B2 (en) 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
WO2012004619A1 (en) 2010-07-07 2012-01-12 Diagon Kft. Procedure for the specific isolation of total dna content of bacterial germs and a kit for this purpose
US20150045232A1 (en) * 2010-12-28 2015-02-12 Bexmart Integrated and versatile methods for systems diagnosis of diseases
CN102533725A (zh) * 2011-09-09 2012-07-04 上海佑科生物技术有限公司 一体化缓冲液及使用该一体化缓冲液分离核酸的方法
WO2013062476A1 (en) * 2011-10-27 2013-05-02 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
US10358641B2 (en) 2014-01-28 2019-07-23 Dice Molecules Sv, Llc Monoliths with attached recognition compounds, arrays thereof and uses thereof
JP2016020826A (ja) * 2014-07-14 2016-02-04 山善株式会社 分取液体クロマトフラフィ用インジェクタ
CA3053576A1 (en) * 2017-02-27 2018-08-30 miDiagnostics NV System and method for purifying and amplifying nucleic acids
JP7719052B2 (ja) * 2019-08-08 2025-08-05 バイオカルティス エン フェー 新規の核酸精製の化学
CN115667510A (zh) * 2020-05-25 2023-01-31 积水医疗株式会社 核酸的纯化方法
CN114573483B (zh) * 2022-03-16 2022-11-01 湖南师范大学 一种疏水的磁性离子液体及其制备方法和应用
CN114990109B (zh) * 2022-06-21 2025-03-25 中国科学院过程工程研究所 一种核糖核酸纯化伴侣及其应用
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
WO2025164403A1 (ja) * 2024-01-30 2025-08-07 株式会社AdvanSentinel 核酸の回収方法及びそれに用いられるキット

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
FI885022L (fi) 1987-03-02 1988-11-01 Jr Lyle John Arnold Polykatjoniska baerare foer rening, isolering och hybridisering av nukleinsyror.
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
JPH06501225A (ja) 1990-07-09 1994-02-10 ジェイ　ガズデン　ピーティーワイ　リミテッド 改良型容器
AU689815B2 (en) 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB9725839D0 (en) * 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
CN1230440C (zh) * 1997-12-06 2005-12-07 Dna研究创新有限公司 核酸的分离
EP0969090A1 (en) 1998-05-27 2000-01-05 QIAGEN GmbH Rapid and simple process for isolation of circular nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
WO2001094573A2 (en) 2000-06-02 2001-12-13 Pall Corporation Processing of plasmid-containing fluids
US6858720B2 (en) * 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US20040121336A1 (en) 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US20040180445A1 (en) 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
AU2003300537A1 (en) * 2003-12-19 2005-07-21 Preanalytix Gmbh Composition for binding a nucleic acid to a solid phase
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
US20070196826A1 (en) * 2004-03-23 2007-08-23 Hiroyuki Ohno Solvent For Dissolving Nucleic Acid, Nucleic Acid-Containing Solution And Method Of Preserving Nucleic Acid
WO2007094739A1 (en) * 2006-02-13 2007-08-23 Agency For Science, Technology And Research Method of processing a biological and/or chemical sample

Also Published As

Publication number Publication date
JP5685227B2 (ja) 2015-03-18
EP1983051A2 (en) 2008-10-22
CA2629589A1 (en) 2008-10-20
CA2629589C (en) 2016-03-29
EP1983051A3 (en) 2008-12-03
JP2008263982A (ja) 2008-11-06
CN101289661B (zh) 2013-08-21
HK1125672A1 (en) 2009-08-14
US8101744B2 (en) 2012-01-24
JP2012255798A (ja) 2012-12-27
US20080319182A1 (en) 2008-12-25
CN101289661A (zh) 2008-10-22
EP1983051B1 (en) 2014-11-26

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