JP5313003B2 - Moisturizer - Google Patents

Description

      The present invention comprises an anti-aging agent, a moisturizing agent, a whitening agent, an antioxidant, It relates to inflammatory agents and skin external preparations.

      Conventionally, in order to provide a moisturizing ingredient that prevents the skin from drying and moisturizes the skin, various active ingredients have been studied. Moreover, as factors of skin symptoms such as aging, diseases, stress, wrinkles due to ultraviolet rays, blemishes, reduced skin elasticity, dryness, cellular function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components, ultraviolet rays Oxidative damage of cells due to the above. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations have been studied.

For example, in order to obtain an external preparation for skin having anti-aging and improving effects on skin, moisturizing effect and safety of skin such as essence of Ponkan (see Patent Document 1) as a component having an activity of dermal fibroblast activation or proliferation. As an excellent moisturizing agent, an extract of the genus Halibut (see Patent Document 2) and the like, as a whitening agent, an extract of the white crane reishi (see Patent Document 3) and the like, and as an antioxidant, the plant of the genus Sarogase As a plant extract having an anti-inflammatory action, such as an extract of sucrose (see Patent Document 4), a dried product of soy leaf, cacao and musk or hot water extract thereof (see Patent Document 5), a solvent extract of a cannaceae plant ( Patent Document 6) and the like are disclosed.
JP 2001-131045 A JP 2007-77072 A JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413 JP 2003-201246 A JP-A-4-270224

      Thus, various naturally derived components have been applied so far. However, among naturally derived components, the effect is not sufficient, and the development of better components has been demanded. Therefore, the present invention is to provide an excellent skin external preparation having an anti-aging effect, a moisturizing effect, a whitening effect, an antioxidant effect, and an anti-inflammatory effect.

      We found that the ingredients obtained from Sonaremgra are excellent in anti-aging effect, moisturizing effect, whitening effect, antioxidant effect, anti-inflammatory effect, anti-aging agent, moisturizing agent, whitening agent, antioxidant, anti-inflammatory agent, It came to provide the skin external preparation.

      ADVANTAGE OF THE INVENTION According to this invention, the anti-aging agent, moisturizer, whitening agent, antioxidant, anti-inflammatory agent, and skin external preparation which have the outstanding effect can be provided by mix | blending the component obtained from Sonaremgra.

      The sonaregrass used in the present invention is a dicotyledonous plant belonging to the genus Rubiaceae and genus Futabamugra, which is a herb or small shrub and is distributed in the south of Chiba Prefecture, Shikoku, Kyushu, and Ryukyu.

      When using Sonaremgra in the present invention, there are no particular restrictions on the site of use, and each site such as leaves, stems, flowers, roots, and whole grasses can be used, but whole grasses are preferably used. .

      During extraction, Sonaremgra may be used as it is, but considering extraction efficiency, it is preferable to perform extraction after performing processing such as shredding, drying, and pulverization.

      The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. In order to increase extraction efficiency, stirring or homogenization in an extraction solvent may be performed. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

      As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used, and water and ethanol are preferable. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

      The solvent extract of Sonaremgra used in the present invention can be used as it is, but it may be left standing for a certain period of time and aged, or the concentrated and dried product may be used as water or a polar solvent. It can be dissolved again and used. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment by column chromatography or the like within a range not impairing these physiological functions. The said extract of plant and shellfish, its processed material, and the fractionation thing can also be freeze-dried after each processing and fractionation, and can also be melt | dissolved and used for a use solvent. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

      Sonaremgra or its extract has excellent anti-aging effect, moisturizing effect, whitening effect, antioxidant effect, anti-inflammatory effect, anti-aging agent, moisturizing agent, whitening agent, antioxidant, anti-inflammatory agent, topical skin application It can be used as an agent.

      An anti-aging agent comprising Sonalemgra or an extract thereof as an active ingredient has an activating effect on human dermal fibroblasts and exhibits an excellent effect in preventing and improving aging symptoms.

      A moisturizing agent containing Sonaremgra or an extract thereof as an active ingredient has a hyaluronic acid production promoting action of human dermal fibroblasts and an arginase activity promoting action of human epidermal keratinocytes, and exhibits an excellent moisturizing effect.

      A whitening agent containing Sonaremgra or an extract thereof as an active ingredient has a B16 mouse melanoma cell melanin production inhibitory action, and prevents and improves pigmentation, spots, freckles, etc., and exhibits an excellent whitening effect.

      An antioxidant containing Sonaremgra or its extract as an active ingredient has a radical scavenging action and a superoxide anion scavenging action, and exhibits an excellent antioxidant effect.

An anti-inflammatory agent comprising Sonaremgra or an extract thereof as an active ingredient has a phospholipase A ₂ inhibitory action and exhibits an excellent anti-inflammatory effect.

      An external preparation for skin containing Sonaremgra or an extract thereof exhibits an excellent anti-aging effect, moisturizing effect, whitening effect, antioxidant effect, anti-inflammatory effect and the like.

      As long as each agent contains Sonaremgra or an extract thereof as an active ingredient, the form and presence / absence of other ingredients are not limited. As for the form, any form such as liquid, paste, gel, solid or powder can be selected according to its use and the like, vehicle (excipient), solvent, Or other general additives (an antioxidant, a coloring agent, a dispersing agent etc.) can be included arbitrarily.

      Here, the external preparation for skin means all external compositions for external use on the skin or hair, such as cosmetics, quasi-drugs or external pharmaceuticals.

      The dosage form of the external preparation for skin is arbitrary, and can be provided, for example, as a solubilization system such as lotion, a dispersion system such as calamine lotion, or an emulsification system such as cream or emulsion. Furthermore, it can also be provided in various dosage forms such as aerosol form, ointment or poultice filled with propellant.

      Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents or makeup cosmetics; liquids, ointments, powders, granules, aerosols, patches or poultices Various forms of cosmetics, quasi-drugs, or external medicines can be exemplified.

      In addition to Sonaremgra or its extract, the external preparation for skin optionally contains any component that is usually blended in pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics, and cleansing agents, etc. Such as water, oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, cleaning agents, UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, chelating agents, drugs (medicinal effects Ingredients), fragrances, resins, fungicides, fungicides, antioxidants, alcohols and the like can be appropriately blended. Furthermore, within the range that does not impair the effects of the present invention, other moisturizers, anti-aging agents, antioxidants, slimming agents, whitening agents, anti-inflammatory agents, immunostimulants, or plants other than Sonaremgra or extracts thereof Is also possible.

      The amount of Sonaremgra or its extract to the external preparation for skin can be adjusted depending on the type, purpose, etc., but from the viewpoint of effect and stability, the total amount is preferably 0.0001 in terms of solid content. To 10.0 mass%, more preferably 0.001 to 5.0 mass%, still more preferably 0.01 to 5.0 mass%, still more preferably 0.1 to 5.0 mass%. %.

      Hereinafter, preparation examples of Sonaremgra extract, anti-aging effect, moisturizing effect, whitening effect, antioxidant effect, test method for evaluating anti-inflammatory effect, formulation example as a skin external preparation will be described in more detail. The technical scope of the present invention is not limited by these.

[Extract 1]
To 100 g of dry pulverized whole plant of Sonaremgra, 2.0 kg of 50 mass% aqueous ethanol solution was added and extracted for 2 hours at room temperature with stirring. The extract was collected by filtration, concentrated under reduced pressure, and lyophilized to obtain extract 1.

      Each effect was evaluated using the said extract. Note that * and ** described in each evaluation result indicate that the significance probability P value in the t-test is less than 5% (P <0.05), and less than 1% significance (P <0.01). ) Is represented by **.

[Example 1]
<Anti-aging effect (evaluation of human dermal fibroblast activation)>
Normal human dermal fibroblasts manufactured by Kurabo Industries Co., Ltd. (Kurashiki Boseki Co., Ltd.) were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After culturing for 24 hours, the medium was replaced with 1% by mass FBS-added DMEM medium to which extract 1 was added so that the concentrations shown in Table 1 were obtained, and further cultured for 48 hours. After removing the supernatant, the medium was replaced with a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) and cultured for about 2 hours. Thereafter, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. In the evaluation, in addition to the medium containing the sample, 1% by mass FBS-added DMEM medium was used as a control. The evaluation results are shown in Table 1 as relative values when the cell activation effect in the control is 100.

      As apparent from the results in Table 1, Sonaremgra (whole plant) 50% ethanol extract (Extract 1) showed a significant anti-aging effect.

[Example 2]
<Moisturizing effect (Evaluation of human dermal fibroblast hyaluronic acid production promoting effect)>
Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the culture medium was replaced with a sample culture solution to which the extract 1 was added so as to have each concentration shown in Table 2 in a DMEM medium supplemented with 0.5 mass% FBS, and further cultured for 3 days. For the quantification of hyaluronic acid secreted into the culture supernatant, an indirect ELISA method using proteoglycan was used, and finally 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid against labeled peroxidase. ) After diammonium salt (ABTS) and hydrogen peroxide were added and reacted, the absorbance at 405 nm was measured with a microplate reader. The amount of protein in each well was measured with a BCA protein assay kit manufactured by PIERCE, and the amount of hyaluronic acid produced per unit protein amount was determined. The evaluation results are shown in Table 2 as relative values with the amount of hyaluronic acid produced per unit protein in the control with no sample added as 100.

[Example 3]
<Moisturizing effect (Evaluation of human epidermal keratinocyte arginase activity promoting effect)>
Human epidermal keratinocytes HaCaT were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. Twenty-four hours later, the sample culture medium was replaced with a 5% by mass FBS-added DMEM medium (differentiation induction medium) containing 1.2 mM CaCl ₂ for each concentration shown in Table 3, and further cultured for 9 days. The medium was exchanged once every 3 days. Urea nitrogen B-Test Wako (Wako Pure Chemical Industries) was used for quantification of urea secreted into the culture supernatant. Arginase hydrolyzes arginine to produce ornithine and urea. Urea is decomposed into ammonia by urease, and ammonia reacts with salicylic acid and hypochlorous acid in the presence of sodium pentacyanonitrosyl iron (III) dihydrate (sodium nitroprusside) to produce indophenol. Absorbance at 570 nm derived from indophenol was measured under alkaline conditions, the urea concentration was determined, and arginase activity was quantified. The protein amount in each well was measured with a BCA protein assay kit manufactured by PIERCE, and the arginase activity per unit protein amount was determined. The evaluation results are shown in Table 3 as relative values when the arginase activity per unit protein amount in the control with no sample added is defined as 100.

      As is clear from the results in Tables 2 and 3, Sonaremgra (whole plant) 50% ethanol extract (Extract 1) showed a significant moisturizing effect.

[Example 4]
<Whitening effect (evaluation of B16 mouse melanoma cell melanin production inhibitory effect)>
B16 mouse melanoma cells (B16F0 cells) were seeded in a 90 mm dish so that there were 18000 cells per dish. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the culture medium was added with the extract 1 to the concentration shown in Table 4 in a DMEM medium supplemented with 5% mass FBS, and further cultured for 5 days. After completion of the culture, cells were peeled off by trypsin treatment, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The obtained precipitate was visually determined based on the following determination table. In the evaluation, a 5% mass FBS-added DMEM medium containing 5% mass FBS was used as a negative control, and a 5% mass FBS-added DMEM medium containing 50 mM sodium lactate was used as a positive control. These naked eye determination results were determined to be determination 5 and determination 1, and used as an index for sample determination. The naked eye evaluation was evaluated in five stages as shown below. At the same time, the tissue solubilizer (trade name Solvable) was added to the precipitate, boiled, returned to room temperature, and the absorbance at 500 nm was measured with a spectrophotometer (HITACHI spectrophotometer U-3010) to determine the total amount of melanin. Asked. The evaluation results are shown in Table 4.
Judgment and Standard Judgment Criterion 1 Same as positive control (almost white)
2 Slightly darker than the positive control (light brown)
3 Between positive control and negative control (brown)
4 Blackening is slightly suppressed compared to the negative control (blackish brown)
5 Same level as negative control (almost black)

      As is clear from the results in Table 4, Sonaremgra (whole plant) 50% ethanol extract (Extract 1) showed a significant whitening effect.

[Example 5]
<Antioxidant effect (evaluation of DPPH radical scavenging action)>
Extract 1 was adjusted to the concentration shown in Table 5 using 50% by mass ethanol to obtain a sample solution, and 100 μL was added to each 96-well microplate. Thereto was added 100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydride radical (DPPH) ethanol solution, mixed well, and allowed to stand at room temperature in the dark for 10 minutes. Thereafter, the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance of the control when the sample was not added was (A) and the absorbance when the sample was added was (B), the DPPH radical elimination rate was calculated by introducing the formula (1). Table 5 shows the measurement results.
Formula (1): Radical scavenging rate = {1- (B) / (A)} × 100 (%)

      As is clear from the results in Table 5, Sonaremgra (whole plant) 50% ethanol extract (Extract 1) showed a significant antioxidant effect.

[Example 6]
<Anti-inflammatory effects (evaluation of phospholipase _{A 2} inhibitory action)>
Phospholipase A ₂ (PLA ₂ ) adjusted to 60 ng / mL, samples prepared to the concentrations shown in Table 6, and DTNB (5,5-dithio-bis- (2-nitrobenzoic acid) adjusted to 10 mM )) Was mixed 10 μL each and allowed to stand at room temperature for 10 minutes. Further, 50 μL of 1.66 mM diheptanoyl thio-PC was added as a substrate, reacted at room temperature for 45 minutes, and the absorbance at 414 nm was measured. In addition, the absorbance at the time of buffer addition was measured instead of the PLA ₂ solution, and the difference between the two measured values was determined. When the value of the control with no sample added is (A) and the value at the time of sample addition is (B), the PLA ₂ inhibitory action is defined by the following equation.
Inhibition rate (%) = {1- (B) / (A)} × 100

      As is clear from the results in Table 6, Sonaremgra (whole plant) 50% ethanol extract (Extract 1) showed a significant anti-inflammatory effect.

      Then, the formulation example of the skin external preparation which mix | blended the Sonaremgra extract obtained by said each preparation method is shown.

[Example 7]
Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) The balance to be purified water 100 (11) Arginine (1% by mass aqueous solution) 20.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After cooling, add (11) and (12) sequentially at 40 ° C. and mix uniformly.

[Example 8]
Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract 1 1.0
Production method: (2) and (3) are dissolved in (1). Further, after sequentially adding (4) to (8), the mixture is sufficiently stirred, and (9) is added and mixed uniformly.

[Example 9]
Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. Add (11), stir, cool and add (12) at 40 ° C. and mix uniformly.

[Example 10]
Essence (1) Purified water 100 balance (mass%)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract 1 3.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. After cooling, add (15) at 50 ° C and (16) at 40 ° C and mix uniformly.

[Example 11]
Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The balance made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract 1 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Example 12]
Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 100 (4) Extract 1 4.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Example 13]
Facial cleansing foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water, the remainder as 100 (8) Extract 1 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. After cooling, add (8) at 40 ° C. and mix uniformly.

[Example 14]
Makeup base cream (1) Squalane 10.2 (mass%)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance which makes 100 purified water (8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract 1 3.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. After cooling, add components (11) and (12) at 40 ° C. and mix uniformly.

[Example 15]
Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) The balance of purified water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract 1 0.5
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. After cooling, components (16) and (17) are added sequentially at 40 ° C. and mixed uniformly.

[Example 16]
Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) The balance of 100 purified water (11) Fragrance 0.1
(12) Extract 1 3.0
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (9) are added to the remainder of (10) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. After cooling, add (11) and (12) at 40 ° C and mix uniformly.

[Example 17]
Pack (1) Remainder water (100%)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract 1 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and cool with stirring. Add (6) and (7) at 40 ° C. and mix uniformly.

[Example 18]
Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract 1 3.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 46.7
Production method: (1) to (4) are mixed uniformly.

[Example 19]
Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 100 (11) Extract 1 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. After cooling, add components (11) and (12) at 40 ° C. and mix uniformly.

[Example 20]
Hair artic (1) Ethanol 50.0 (mass%)
(2) The balance of purified water 100 (3) Extract 1 3.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

      The skin external preparations shown in Examples 7 to 20 were compositions having an anti-aging effect, a moisturizing effect, a whitening effect, an antioxidant effect, and an anti-inflammatory effect.

      The components obtained from the Sonaremgra of the present invention are useful for blending into skin external preparations such as skin cosmetics, hair cosmetics or cleansing agents, pharmaceuticals and quasi drugs. Further, the Sonaremgra extract according to the present invention is a naturally derived component, so it is easily considered to be highly safe and has great significance as a skin external preparation. Therefore, the present invention is a new skin external preparation. Useful as.

Claims (1)

  A moisturizer characterized by containing Sonaremgra (Hedyotis strigulosa var. Parvifolia) or an extract thereof.