JP5138316B2 - Plant cultivation method - Google Patents

Description

  The present invention provides a predetermined plant stress tolerance imparting agent or plant stress tolerance imparting composition to plants in a stress environment, for example, foliar spraying on plant roots, stems, leaves or fruits, soil irrigation, etc. It is related with the plant stress tolerance imparting method applied by the method. Here, hereinafter, the “plant” represents one that can be recognized from the wording of the plant itself, vegetable, fruit, fruit tree, grain, seed, bulb, flower, herb (herb), taxonomic plant, etc. .

  About one-third of the land on the earth belongs to arid land, and further arid land is expected to increase due to future warming. In addition, as a countermeasure against serious food shortages due to population growth, yields are improved in dry areas, salt accumulation areas, areas where temperatures are high and low, that is, areas where growth is difficult or growth has deteriorated and yield has declined. There is an urgent need to develop, maintain and increase technology.

  When plants grow in nature or artificial environments, temperature (high temperature, low temperature, freezing), strong wind, light intensity (strong light, weak light), drying, inorganic toxicity (salts, heavy metals, aluminum, etc.), oxygen, Subject to various stresses such as machines and pests. However, plants cannot protect themselves from various stresses by moving like animals. In order to acquire stress tolerance, plants are known to synthesize various substances in vivo when stressed. For example, it is a compatible solute such as proline, glycine betaine, and sugar (Non-Patent Document 1). In addition, it is known that when subjected to the stress described above, plants produce aging hormones such as abscisic acid, and the growth decreases or stops, resulting in a decrease in yield.

As methods for improving the stress tolerance of such plants, methods such as selection and breeding, gene recombination (patent documents 1 and 2), drugs such as glycine betaine (patent document 3), aminolevulinic acid (patent document 4), Although there is application of silicic acid (Non-Patent Document 2), these effects cannot be obtained enough to slightly relieve stress, and none are currently in practical use. In addition, it is known to use a specific compound such as stearyl alcohol as a low temperature tolerance improver for agricultural products (Patent Document 5).
JP 2002-262885 A JP 2002-369634 A JP-A-10-262457 JP-A-8-151304 JP 2002-265307 A “Protein Nucleic Acid Enzyme” (Kyoritsu Shuppan) vol. 44 no. 15 PP54-65 1999 “Study of Roots” (Root Research Society) vol. 14 (2): PP41-49 2005

  An object of the present invention is to provide a method of imparting stress resistance to plants that promotes growth even in an environment in which various stresses on the plants occur.

  The present invention applies (A) a plant stress tolerance imparting agent comprising a compound represented by the following general formula (1) to a plant under cultivation conditions having a plant stress rate of 111 to 200% (low temperature) (Excluding tolerance).

(In the formula, R ¹ is a hydrocarbon group having 10 to 22 carbon atoms, R ² is a hydrogen atom, a hydroxyl group or a hydrocarbon group having 1 to 24 carbon atoms, R ³ is a hydrogen atom or a hydrocarbon group having 1 to 24 carbon atoms. Represents.)

  The present invention also relates to (A) a plant stress tolerance imparting agent comprising a compound represented by the above general formula (1), or (A) a compound represented by the above general formula (1) [hereinafter referred to as compound (A). )], And (B) one or more surfactants selected from sugar derivative surfactants and sugar alcohol derivative surfactants (hereinafter referred to as compound (B)), (C) silicon compounds (hereinafter referred to as compounds) (Referred to as (C)), (D) betaine (hereinafter referred to as compound (D)), (E) amino acid (excluding tryptophan) (hereinafter referred to as compound (E)), (F) antioxidant (hereinafter referred to as compound ( F)], (G) auxin biosynthetic pathway intermediate substance (biosynthetic pathway from shikimic acid to indoleacetic acid) [hereinafter referred to as compound (G)] and (H) saccharide [hereinafter referred to as compound (H)] Selected from the group consisting of One or more compounds, a plant stress tolerance imparting composition containing that plant stress rate is applied to the plant in the 111 to 200% of the cultivation conditions, relating to plant stress tolerance imparting method.

  ADVANTAGE OF THE INVENTION According to this invention, tolerance with respect to various stress, such as salt stress, temperature stress, and drought stress, can be provided with respect to a plant. Therefore, the growth of the plant can be promoted even in an environment where various stresses occur on the plant.

<Compound (A)>
In the general formula (1), the hydrocarbon groups of R ¹ , R ² and R ³ may be either saturated or unsaturated, preferably saturated, and may be linear, branched or cyclic, Preferably it is a straight chain or branched chain, more preferably a straight chain. The total carbon number of the hydrocarbon group may be an odd number or an even number, but an even number is preferable.

Also, the total number of carbon atoms of R ^1, R ^2, R ³ are all preferably 50 or less, more preferably 12 to 48, more preferably from 16 to 44.

In the general formula (1), the carbon number of R ¹ is preferably 14 to 22, more preferably 14 to 20, more preferably from 14 to 18. Further, the compound represented by the general formula (1) preferably has a total carbon number of 12 to 48, more preferably 16 to 28, and even more preferably 16 to 24. Further, those having a total carbon number of 12 to 24 and one hydroxyl group are preferred, and those having a total carbon number of 16 to 22 and one hydroxyl group are more preferred. Specific examples of the compound represented by the general formula (1) include the following.

(A1)
1-alkanol represented by CH ₃ (CH ₂ ) _s-1 OH (s is an integer of 12 to 24, preferably 16 to 24, more preferably 16 to 20). That is, as the compound represented by the general formula (1), a monohydric alcohol having 12 to 24 carbon atoms can be given. Specifically, 1-dodecanol, 1-tridecanol, 1-tetradecanol, 1-pentadecanol, 1-hexadecanol, 1-heptadecanol, 1-octadecanol, 1-nonadecanol, 1-icosanol 1-henicosanol, 1-docosanol, 1-tricosanol, 1-tetracosanol.

(A2)
2-alkanol represented by CH ₃ CH (OH) (CH ₂ ) _p-3 CH ₃ (p is an integer of 12 to 24, preferably 16 to 24, more preferably 16 to 20). Specifically, 2-dodecanol, 2-tridecanol, 2-tetradecanol, 2-pentadecanol, 2-hexadecanol, 2-heptadecanol, 2-octadecanol, 2-nonadecanol, 2-icosanol Etc.

(A3)
_{CH 2 = CH (CH 2)} q-2 OH (q is 12 to 24, preferably 16 to 24, more preferably an integer of 16 to 20) include terminally unsaturated alcohol represented by. Specific examples include 11-dodecene-1-ol, 12-tridecene-1-ol, 15-hexadecene-1-ol, and the like.

(A4)
Examples of other unsaturated long chain alcohols include oleyl alcohol, elaidyl alcohol, linoleyl alcohol, linoleenyl alcohol, eleostearyl alcohol (α or β), ricinoyl alcohol, and the like.

(A5)
_{HOCH 2 CH (OH) (CH} 2) r-2 H (r is 12 to 24, preferably 16 to 24, more preferably an integer of 16 to 20) include 1,2-diol represented by. Specific examples include 1,2-dodecanediol, 1,2-tetradecanediol, 1,2-hexadecanediol, 1,2-octadecanediol, and the like.

  Of the above (A1) to (A5), (A1), (A2), (A4), and (A5) are preferable, and (A1), (A2), and (A4) are more preferable. Preferably, (A1) and (A4) are more preferable, and (A1) is still more preferable.

  In order to apply the plant stress tolerance imparting agent comprising the compound (A) to the above-ground part or underground part of the plant, a treatment liquid containing the compound (A), preferably a treatment liquid containing water, can be used. . In that case, in the case of foliar spraying, the concentration of the compound (A) in the treatment liquid is preferably 0.1 to 5000 ppm, more preferably 1 to 1000 ppm, and even more preferably 10 to 500 ppm, and the compound (A) per spraying The application amount is preferably 0.01 g / 10 are (hereinafter abbreviated as 10 a) to 500 g / 10 a, more preferably 0.1 g / 10 a to 100 g / 10 a, and even more preferably 1 g / 10 a to 50 g / 10 a. From the viewpoint of imparting resistance to plant stress, it is preferable to adjust the concentration of the compound (A) in the treatment liquid so that the application rate is within this range.

  Moreover, in the case of soil irrigation, the concentration of the compound (A) in the treatment liquid is preferably 0.1 to 1000 ppm, more preferably 1 to 500 ppm, and still more preferably 10 to 200 ppm, and the concentration of the compound (A) per irrigation The application rate is preferably 0.3 g / 10a to 3000 g / 10a, more preferably 3 g / 10a to 1500 g / 10a, and even more preferably 30 g / 10a to 600 g / 10a. From the viewpoint of imparting resistance to plant stress, it is preferable to adjust the concentration of the compound (A) in the treatment liquid so that the application rate is within this range.

  Moreover, when applying with a granule, the application amount of the compound (A) per time is 0.1 g / 10a to 1000 g / 10a, further 10 g / 10a to 5000 g / 10a, further 50 g / 10a to 2500 g / 10a, Furthermore, 100g / 10a-1200g / 10a, Furthermore, 300g / 10a-1000g / 10a are preferable.

<Compound (B)>
Compound (B) is one or more surfactants selected from a sugar derivative-type surfactant and a sugar alcohol derivative-type surfactant, and the sugar or sugar alcohol has any of an ester bond, glycoside bond, or amide bond with a hydrophobic group. A surfactant having a structure containing one bond is preferred. Examples of those having a structure in which a hydrophobic group is ester-bonded to sugar or sugar alcohol include sorbitan fatty acid ester, polyoxyalkylene sorbitan fatty acid ester, sucrose fatty acid ester, sorbit fatty acid ester, polyoxyalkylene sorbit fatty acid ester, polyglycerin, polyglycerin Examples include fatty acid esters, glycerin fatty acid esters, and polyoxyalkylene glycerin fatty acid esters.

  Also, those having a structure in which a hydrophobic group is glycosidically bonded to sugar or sugar alcohol include alkyl glycosides, alkyl polyglycosides, polyoxyalkylene alkyl (poly) glycosides, and alkyl (poly) glycosides obtained by sulfating alkyl (poly) glycosides. Sulfates, phosphorylated alkyl (poly) glycosides, glyceryl etherified alkyl (poly) glycosides, sulfosuccinate esterified alkyl (poly) glycosides, glyceryl esterified alkyl (poly) glycosides, carboxyalkylated alkyl (poly) glycosides, cationized alkyls Examples include (poly) glycosides and betaylated alkyl (poly) glycosides.

Further, those having a structure in which a hydrophobic group is amide-bonded to sugar or sugar alcohol, for example, sugar fatty acid amides such as glucose and fructose fatty acid amides can be used. Further, those having a structure in which a hydrophobic group is amide-bonded to a sugar or sugar alcohol having an amino group, for example, a sugar fatty acid amide such as a fatty acid amide of N-methylglucamine can be used. As the sugar fatty acid amide, the following general formula (2)
R ₁₁ —CO—NR ₁₂ X (2)
[Wherein, R ₁₁ is a linear or branched alkyl group or alkenyl group having 5 to 17 carbon atoms, or an alkyl (carbon number 1 to 13) phenyl group, and R ₁₂ is a hydrogen atom or carbon number 1 to 18 A linear or branched alkyl group or alkenyl group, — (CH ₂ CH (R ₁₃ ) O) _c —H (where R ₁₃ is a hydrogen atom or a methyl group, and c is a number from 0 to 10. -CH ₂ CH ₂ OH, -CH ₂ CH (OH) CH ₃ or -CH ₂ CH ₂ CH ₂ OH, and X is a polyhydroxyalkyl group consisting of a sugar residue having 4 to 30 carbon atoms. is there. ]
The compound represented by can be preferably used.

R ₁₁ in the formula (2) is a linear or branched alkyl or alkenyl group having 5 to 17 carbon atoms or an alkylphenyl group, and R ₁₁ CO is capric acid, caprylic acid, lauric acid, myristic acid. And groups derived from palmitic acid, stearic acid, and isostearic acid, and groups derived from capric acid and lauric acid are preferred.

Specific examples of R ₁₂ include hydrogen atom, methyl group, ethyl group, n-propyl group, isopropyl group, n-butyl group, t-butyl group, n-hexyl group, octyl group, 2-ethylhexyl group, decyl group. , Dodecyl group, stearyl group, isostearyl group, polyethylene glycol group or polypropylene glycol group having a polymerization degree of 2 to 10, 2-hydroxyethyl group, 2-hydroxypropyl group, 3-hydroxypropyl group, and the like. Among these, a hydrogen atom, a methyl group, an ethyl group, a 2-hydroxyethyl group, a 2-hydroxypropyl group, and a 3-hydroxypropyl group can be preferably exemplified.

  The polyhydroxyalkyl group consisting of a sugar residue of 4 to 30 carbon atoms in X includes a 4 to 7 carbon polyhydroxyalkyl group that is glycosidically bonded to a mono-, di-, or oligosaccharide group.

  As the compound (B), sorbitan fatty acid ester, polyoxyalkylene sorbitan fatty acid ester, alkyl polyglycoside, and sucrose fatty acid ester are more preferable from the viewpoint of imparting plant stress resistance.

  The sorbitan fatty acid ester preferably has a high monoester ratio, and the HLB (Hydrophilic Lyophilic Balance) is preferably in the range of 3-10. The acyl group constituting the hydrophobic group may be saturated, unsaturated, linear or branched, but preferably has 8 to 18 carbon atoms from the viewpoint of imparting plant stress resistance.

  As an alkyl polyglycoside, it is preferable that average sugar condensation degree is 1.1-5.0, and it is still more preferable that it is 1.1-2.0 from a viewpoint of plant stress tolerance provision. Further, those having a glucose skeleton as the sugar skeleton and an average sugar condensation degree of 1.1 to 2.0 are preferred. The hydrophobic group may be saturated, unsaturated, linear or branched, but preferably has 8 to 18 carbon atoms, and more preferably has 8 to 14 carbon atoms.

  The sucrose fatty acid ester is a mixture of mono, di, tri, and polyester (tetraester or higher), but from the viewpoint of imparting plant stress resistance, the monoester and diester content is high, the polyester content is low, and the HLB is 4-18. It is preferable that it is the range of these. The acyl group constituting the hydrophobic group may be saturated, unsaturated, linear or branched, but preferably has 8 to 18 carbon atoms.

  The concentration of the compound (B) in the treatment solution is preferably 0.01 to 10000 ppm, more preferably 0.1 to 5000 ppm, and more preferably 1 to 2000 ppm as the concentration when applied to the plant body when spraying the leaves. More preferred. When applied from the underground in soil and hydroponics, 0.01 to 10000 ppm is preferable, 0.1 to 2000 ppm is more preferable, and 1 to 1000 ppm is more preferable.

<Compound (C)>
Compound (C) is a silicon compound, inorganic silicon compounds such as silicon, silicic acid, orthosilicic acid, disilicic acid, trisilicic acid, polysilicic acid and silane, silicone oil, organic silane, siloxide, silylhydride, silene, silicone surface activity And organosilicon compounds such as agents. Among inorganic silicon compounds, silicon, silicic acid, orthosilicic acid, disilicic acid, trisilicic acid, and polysilicic acid are preferred, and silicic acid, orthosilicic acid, disilicic acid, and trisilicic acid are more preferred. The silicone oil is preferably mixed with a surfactant and dispersed in water. In the organosilicon compound, a silicone surfactant is preferable, and polyoxyethylene methyl polysiloxane is more preferable.

  Accordingly, the compound (C) is one or more selected from the group consisting of silicon, silicic acid, orthosilicic acid, disilicic acid, trisilicic acid, polysilicic acid, silicone oil, and silicone surfactant from the viewpoint of imparting plant stress resistance. Compounds are preferred.

  The concentration of the compound (C) in the treatment solution is preferably 0.01 to 10000 ppm, preferably 0.1 to 5000 ppm from the viewpoint of imparting plant stress resistance, when the foliar spray is applied as a concentration when applied to the plant body. More preferably, it is more preferably 1 to 2000 ppm. When applying from the underground in soil and hydroponics, 0.01 to 10000 ppm is preferable, 0.1 to 2000 ppm is more preferable, and 1 to 1000 ppm is more preferable from the viewpoint of imparting plant stress resistance.

<Compound (D)>
Compound (D) is a betaine compound, and examples thereof include glycine betaine, proline betaine, alanine betaine, and betaine surfactants. As betaine surfactants, alkyl dimethyl betaine, alkyl hydroxyethyl betaine, acylamidopropyl hydroxypropyl ammonia sulfobetaine, acylamidopropyl hydroxypropyl ammonia sulfobetaine, ricinoleic acid amidopropyl dimethylcarboxymethyl ammonia betaine, imidazoline series, Examples thereof include alkyl carboxymethyl hydroxyethyl imidazolinium betaine and alkyl ethoxy carboxymethyl imidazolium betaine. From the viewpoint of imparting plant stress resistance, glycine betaine and alkyldimethylbetaine are preferable, and the alkyl group of alkyldimethylbetaine preferably has 8 to 24 carbon atoms. The compound (D) is preferably one or more compounds selected from the group consisting of glycine betaine and alkyldimethylbetaine.

  The concentration of the compound (D) in the treatment solution is preferably 0.1 to 5000 ppm, more preferably 0.1 to 1000 ppm from the viewpoint of imparting plant stress resistance, when the foliar spray is applied as a concentration when applied to the plant body. More preferably, 1 to 500 ppm is more preferable. When applying from the underground in soil and hydroponics, 0.1 to 10,000 ppm is preferable, 0.1 to 2000 ppm is more preferable, and 1 to 1000 ppm is more preferable from the viewpoint of imparting plant stress resistance.

<Compound (E)>
Compound (E) is an amino acid excluding tryptophan, such as alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, glycine, serine, threonine, cysteine, glutamine, asparagine, tyrosine, lysine, histidine, aspartic acid, glutamic acid, etc. Can be mentioned. From the viewpoint of imparting plant stress tolerance, one or more compounds selected from the group consisting of proline, glutamic acid and aspartic acid are preferred.

  The concentration of the compound (E) in the treatment solution is preferably 0.1 to 10000 ppm, more preferably 1 to 5000 ppm from the viewpoint of imparting plant stress resistance when spraying leaves as the concentration when applied to the plant body. Furthermore, 10 to 1000 ppm is more preferable. When applying from the underground part in soil and hydroponics, 0.01 to 5000 ppm is preferable, 0.1 to 1000 ppm is more preferable, and 1 to 500 ppm is more preferable from the viewpoint of imparting plant stress resistance.

<Compound (F)>
Compound (F) is an antioxidant, and includes antioxidants, plant antioxidants, plant-derived antioxidants, and the like. Furthermore, examples of plant-derived antioxidants include vitamins, carotenoids, phenols, terpenes, and polyphenols. Specifically, examples of the antioxidant include dibutylhydroxytoluene (BHT), butylhydroxyanisole (BHA), ascorbic acid, guaiac fat, oryzanol, sulfurous acid, hydrogen sulfite, n-propyl gallate, hydrogen phosphite, and the like. Examples of plant antioxidants include superoxide dismutase, catalase, and glutathione peroxidase. Plant-derived antioxidants include carotene, zeaxanthin, glutathione, caffeine, flavone, quercetin, rutin, chlorophyll, saponin, cafe In, catechin, polyphenol, sesamin, sesamol, astaxanthin, curcumin, ascorbic acid, α-tocopherol and the like. From the viewpoint of imparting plant stress tolerance, more preferably one or more selected from the group consisting of flavone, quercetin, rutin, chlorophyll, saponin, caffeine, catechin, polyphenol, sesamin, sesamol, astaxanthin, curcumin, ascorbic acid and α-tocopherol More preferred are quercetin, saponin, catechin, and α-tocopherol.

  The concentration of the compound (F) in the treatment solution is preferably 0.01 to 5000 ppm, more preferably 0.1 to 1000 ppm from the viewpoint of imparting plant stress resistance, when the foliar spray is applied as a concentration when applied to the plant body. More preferably, 1 to 500 ppm is more preferable. When applying from the underground part in soil and hydroponics, 0.01 to 5000 ppm is preferable, 0.05 to 1000 ppm is more preferable, and 0.1 to 500 ppm is more preferable, from the viewpoint of imparting plant stress resistance.

<Compound (G)>
Compound (G) is an auxin biosynthetic pathway intermediate substance in the biosynthetic pathway from shikimic acid to indoleacetic acid in the plant body. Shikimic acid, chorismic acid, anthranilic acid, tryptophan, indole-3-pyruvic acid, indole- Examples include 3-acetaldehyde, indole-3-acetoaldoxime, indole-3-methylglucosinolate, indole-3-acetonitrile, tryptamine, and indole acetic acid. From the viewpoint of imparting plant stress tolerance, one or more compounds selected from the group consisting of shikimic acid, anthranilic acid, tryptophan, and indoleacetic acid are more preferred.

  The concentration of the compound (G) in the treatment solution is preferably 0.01 to 5000 ppm, more preferably 0.1 to 1000 ppm from the viewpoint of imparting plant stress resistance when spraying leaves as the concentration when applied to the plant body. More preferably, 1 to 500 ppm is more preferable. When applying from the underground part in soil and hydroponics, 0.01 to 5000 ppm is preferable, 0.05 to 1000 ppm is more preferable, and 0.1 to 500 ppm is more preferable, from the viewpoint of imparting plant stress resistance.

<Compound (H)>
Compound (H) is a saccharide, glycerol, erythritol, erythrulose, erythrose, threose, xylose, ribose, arabinose, lyxose, deoxyribose, ribulose, arabitol, fructose, inositol, rhamnose, mannitol, sorbitol, glucose, gluconic acid, mannose , Altrose, idose, galactose, quinobose, glucaric acid, gulose, digitalose, digitoxose, cimarose, sorbose, tagalose, talose, fucose, psicose, galactitol, iduronic acid, galacturonic acid, glucuronic acid, mannuronic acid, galactosamine, glucosamine, Fucosamine, mannosamine, muramic acid, curdlan, maltitol, trehalose, melibiose, sucrose , Lactose, palatinose, agarobiose, isomaltose, xylobiose, gentiobiose, cordiobiose, chondroucine, cellobiose, sophorose, nigerose, hyalobiuronic acid, maltose, lactulose, laminaribiose, lutinose, glucosyl sucrose, raffinose, gentianose, Triose, melendiose, stachyose, xylo-oligosaccharide, isomaltoligosaccharide, gentio-oligosaccharide, fructooligosaccharide, chitosan oligosaccharide, chitin oligosaccharide, cellooligosaccharide, cyclodextrin, pectin, starch, agarose, amylose, amylopectin, arabinan, arabinogalactan, Alginate, inulin, galactan, xylan, chitin, chitosan, glycogen, Rukoman'nan, keratan sulfate, colominic acid, chondroitin, chondroitin sulfate, cellulose, dextran, hyaluronic acid, pectin, pectic acid, heparan sulfate, heparin, mannan, lichenan, levan, and the like lentinan. From the viewpoint of imparting plant stress resistance, one or more compounds selected from the group consisting of trehalose, raffinose, fructose, glucosyl sucrose, cyclodextrin, mannitol and oligosaccharides are more preferred.

  The concentration of the compound (H) in the treatment liquid is preferably 0.1 to 10000 ppm, more preferably 1 to 5000 ppm from the viewpoint of imparting plant stress resistance when spraying leaves as the concentration when applied to the plant body. Furthermore, 10 to 1000 ppm is more preferable. In the case of applying from the underground in soil and hydroponics, 0.1 to 10,000 ppm is preferable, 1 to 5000 ppm is more preferable, and 10 to 1000 ppm is more preferable from the viewpoint of imparting plant stress resistance.

  In the present invention, a plant stress tolerance imparting agent comprising the compound (A) or a plant stress tolerance imparting agent composition containing the compound (A) and at least one of the compounds (B) to (H) is applied to a plant. Thus, even in an environment where stress occurs, stress tolerance that can promote the growth of plants is imparted.

In general, for cultivated plants such as agricultural crops, suitable cultivation conditions are known for each plant. When a plant is cultivated under such appropriate cultivation conditions or conditions close thereto, the plant is not stressed. In the present invention, whether the plant is stressed is determined by the following plant stress rate. That is, the weight of the plant body (plant body weight 1, the weight of the plant body cultivated under stress) when cultivated under conditions that can cause stress such as salt, drying, and temperature to exceed appropriate values, From the plant weight (plant weight 2, weight of plant grown under non-stress) when cultivated under appropriate conditions (stress not applied) excluding stress factors from the conditions, the following The plant stress rate (%) is calculated by the formula (ii), and when this value is 111% or more, it means that the growth is reduced by 10% (weight basis) or more, and under the cultivation conditions under stress. It is determined that there is. The present invention is applied to a plant having a plant stress rate of 111 to 200%. Furthermore, when applied to a plant having a plant stress rate of 120 to 180%, preferably 120 to 160%, a more remarkable effect is obtained from the viewpoint of imparting plant stress resistance. Note that the plant stress rate can be calculated using a result obtained by reproducing a condition excluding the stress factor while paying attention to a predetermined stress factor in an actual cultivation condition and at a laboratory level.
Plant stress rate (%) = plant weight 2 / plant weight 1 × 100 (ii)

Whether or not stress tolerance is imparted to a plant is confirmed by the above-mentioned plant stress rate that the plant is in a cultivation condition that gives stress, and the plant weight of the plant cultivated under that condition (plant weight 1) And plant weight of a plant cultivated by applying the plant stress tolerance imparting agent or plant stress tolerance imparting agent composition of the present invention from the underground part or the ground part (plant weight 3, stress applied to plants grown under stress) Based on the weight of the plant subjected to the tolerance imparting treatment), the plant stress tolerance imparting rate (%) is calculated by the following formula (iii). If the plant stress tolerance imparting rate exceeds 100, stress tolerance is imparted to the plant, but it is preferably 105% or more, more preferably 111% or more.
Plant stress tolerance imparting rate (%) = plant weight 3 / plant weight 1 × 100 (iii)

  By applying the plant stress tolerance imparting method or plant stress tolerance imparting agent or plant stress tolerance imparting agent composition of the present invention, cultivation is performed under cultivation conditions having stress factors such as salt, temperature, and drying under the conditions of the examples described later. Even if it did, the plant stress tolerance provision rate exceeding 115% can be achieved.

  In the present invention, as a criterion for determining whether or not a specific compound can impart stress tolerance, the standard plant salt stress tolerance imparting rate according to the following standard test is preferably 111% or more. In actual cultivation such as in the field, various stresses are applied to plants, but this standard test identifies the stressed environment and reproduces it at the laboratory level to test the effect of imparting stress tolerance to the test compound. is there. The plant stress tolerance imparting agent or the plant stress tolerance imparting agent composition having a standard plant salt stress tolerance imparting rate of preferably 111% or more can be applied to the above-ground part or underground part of the plant. A standard test for measuring the standard plant salt stress tolerance imparting rate (here, control group 2 is also prepared) is described below.

<Standard test>
(I) Preparation of plant Culture soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / culture soil 1 kg) is packed in a 50-well cell tray and tomato “Momotaro” (Takii seedling) Seeds, cover the culture soil thinly, irrigate with sufficient water and germinate. When the leaves of the second leaf stage are fully developed, the soil at the root of the tomato is washed away with running water and used for the test. As cultivation medium, Kureha gardening cultivation soil made by Kureha Chemical Co., Ltd. can be used.
(II) Setting of test conditions Environmental conditions are controlled at a temperature of 23 ° C., a relative humidity of 50%, an illuminance of 5000 Lux, and a light / dark cycle of 16 hours for a light period of 8 hours and a dark period of 8 hours. Such environmental conditions can be obtained, for example, by adjusting the temperature in a room or an artificial meteor that can control the temperature and relative humidity, and by adjusting the illuminance with a fluorescent lamp or the like. The tomato according to the above preparation is planted in a hydroponic solution (tap water added with NaCl to a concentration of 3510 ppm (water potential of 0.29 MPa with NaCl)) in a container (for example, a polyethylene container).
(III) Treatment with plant stress tolerance imparting agent The following test group, control group 1 and control group 2 are prepared. In each of the test group, the control group 1 and the control group 2, 10 individuals (30 individuals in total) are prepared, and the raw weight of the whole plant after 2 weeks is measured.
Test plot: An aqueous solution or an aqueous dispersion of the plant stress tolerance imparting agent or the plant stress tolerance imparting agent composition [100 ppm as the concentration of the compound (A) represented by the general formula (1)], 10 ml per leaf of tomato, leaf Sprinkle on the surface.
Control group 1: NaCl is added to the hydroponic solution (salt stress is applied), but the plant stress tolerance imparting agent or the plant stress tolerance imparting agent composition is not imparted to the tomato.
Control group 2: NaCl is not added to the hydroponic solution (no salt stress is applied), and the plant stress tolerance imparting agent or the plant stress tolerance imparting agent composition is not imparted to the tomato.
(IV) Calculation of standard plant salt stress tolerance imparting rate (%) The standard plant salt stress tolerance imparting rate is calculated as follows using the average value of the raw weight of the whole plant obtained [Formula (i)].
Standard plant salt stress tolerance application rate (%) = Plant weight of test body / Plant weight of control group 1 × 100 (i)

Note that the plant stress rate (standard plant salt stress rate) in the standard test is around 130%. In this case, the standard plant salt stress rate can be calculated by the following formula (ii) ′.
Standard plant salt stress rate (%) = Plant weight of control group 2 / Plant weight of control group 1 × 100 (ii) ′

  In the examples described later, the standard plant salt stress rate in the standard test was 130%. Moreover, the standard plant salt stress tolerance provision rate by the said standard test of the compound used in the below-mentioned Example was as Tables 1-3 showed.

  Examples of plant stress factors to which tolerance can be imparted according to the present invention include salt stress, drought stress, and temperature stress. That is, the method of the present invention includes at least one stress factor of salt stress caused by salt concentration in soil or culture solution, drought stress caused by moisture content in soil, and temperature stress caused by temperature of cultivation environment. Therefore, it can be applied to plants that have been cultivated with a plant stress rate of 111 to 200%. However, the plant stress tolerance imparting agent comprising the compound (A) is used for imparting stress tolerance excluding low temperature tolerance.

In soil cultivation and hydroponics, the accumulation of salts such as fertilizers increases the osmotic pressure in the cultivation solution and inhibits water absorption of the plant, resulting in a phenomenon in which growth is inhibited. Such a state is generally recognized as a state in which the plant is subjected to salt stress. Specifically, for example, the osmotic potential due to the salt of the hydroponics in hydroponics or the osmotic potential due to the salt in the soil in soil cultivation is 0.2 MPa (2400 ppm in NaCl concentration) or more, and further 0.25 MPa or more. Further, it can be said that the condition is salt stress at 0.30 MPa or more. According to the present invention, it is possible to impart resistance to the proper growth of plants even under such conditions that show osmotic pressure potential. In soil cultivation, the osmotic pressure potential is calculated by the following Raoul's law by diluting the soil with water and analyzing the salt concentration of the supernatant.
Raoul's law π (atm) = cRT
R = 0.082 (L · atm / mol · K)
T = absolute temperature (K)
c = ion molar concentration (mol / L)
1 atm = 0.1 MPa

  In soil cultivation, the moisture content in the soil decreases due to a decrease in the amount of rainfall and irrigation, and the water absorption of the plant is inhibited, resulting in a phenomenon that the growth is inhibited. Such a state is generally recognized as a state in which drought stress is applied to the plant. Specifically, the pF value of the soil where the plant is cultivated is 1.7 or more, which means a state where gravity water cannot be recognized as soil water, more than 2.3, and even more than 2.5. It can be said that this is a condition with drought stress. According to the present invention, it is possible to confer tolerance that a plant grows properly even under such a condition showing a pF value. Here, the pF value can be measured according to the principle described in the “pF value measurement method” on pages 61 to 62 of “Soil / Plant Nutrition / Environment Encyclopedia” (Taiyosha, 1994, Matsuzaka et al.). it can.

  In addition, when a plant is exposed to a temperature higher or lower than the optimum growth temperature of a certain plant with respect to the cultivation environment, a phenomenon occurs in which the physiological metabolic function in the living body is reduced and growth is inhibited. Such a state is generally recognized as a state in which a plant is subjected to temperature stress. Specifically, the average cultivation temperature in the environment where the plant is cultivated is 25 ° C. or higher, further 28 ° C. or higher, more preferably 32 ° C. or higher, or 20 ° C. or lower, further 17 ° C. or lower, and even more. It can be said that it is conditions with temperature stress as it is 15 degrees C or less. According to the present invention, it is possible to impart resistance to the proper growth of plants even under such conditions that indicate the average cultivation temperature. Here, the average cultivation temperature is an average value of cultivation temperatures measured every hour in the cultivation period (period from sowing to the end of growth) regardless of day or night. The plant stress tolerance imparting agent composition containing the compound (A) and one or more of the compounds (B) to (H) has an average cultivation temperature of 20 ° C. or lower, further 17 ° C. or lower, and more preferably 15 ° C. or lower. It is also applicable to cultivation conditions that are considered to be subject to temperature stress.

  As described above, in the present invention, from the viewpoint of imparting plant stress tolerance, a plant stress tolerance imparting agent comprising the compound (A), or the compound (A), the compound (B), the compound (C), and the compound (D). A plant stress tolerance imparting agent composition containing at least one compound selected from the group consisting of a compound (E), a compound (F), a compound (G) and a compound (H) is used. The content of the compound (A) in the plant stress tolerance imparting agent composition is preferably 0.1 to 50% by weight, more preferably 1 to 10% by weight, from the viewpoint of imparting plant stress resistance. From the same viewpoint, the content of the compound (B) is preferably 0.1 to 25% by weight, more preferably 1 to 10% by weight, and the content of the compound (C) is 0.1 to 25% by weight, The content of the compound (D) is further preferably 0.1 to 25% by weight, more preferably 1 to 10% by weight, and the content of the compound (E) is 0.1 to 25% by weight. %, More preferably 1 to 10% by weight, and the content of the compound (F) is preferably 0.1 to 25% by weight, more preferably 1 to 10% by weight, and the content of the compound (G) is 0.1 to 10% by weight. 25% by weight, more preferably 1 to 10% by weight, and the content of the compound (H) is preferably 0.1 to 25% by weight, more preferably 1 to 10% by weight. It is preferable to prepare a treatment solution containing each component at the aforementioned concentration and apply it to a plant.

<Surfactant>
In the present invention, a surfactant (except for those belonging to the compounds (A) to (H)) can be used together with the compound (A) and further the compounds (B) to (H). By using a surfactant, the wettability, adhesion, and permeability of the compound (A) to the plant surface are dramatically improved, the effect of the compound (A) is enhanced, or the effect is efficiently exhibited. The use concentration of the compound (A) can be reduced.

  Nonionic surfactants include resin acid esters, polyoxyalkylene resin acid esters, polyoxyalkylene alkyl ethers, polyoxyalkylene alkyl phenyl ethers, alkyl alkanolamides, and the like.

  Examples of the anionic surfactant include carboxylic acid-based surfactants, sulfonic acid-based surfactants, sulfate ester-based surfactants, and preferably one or more selected from carboxylic acid-based surfactants and phosphate ester-based surfactants. .

  Examples of the carboxylic acid-based surfactant include fatty acids having 6 to 30 carbon atoms or salts thereof, polyvalent carboxylates, polyoxyalkylene alkyl ether carboxylates, polyoxyalkylene alkylamide ether carboxylates, rosinates, Examples include dimer acid salts, polymer acid salts, tall oil fatty acid salts, and esterified starches. Of these, esterified starch, and alkenyl succinylated starch are preferred.

  Examples of the sulfonic acid surfactant include alkylbenzene sulfonate, alkyl sulfonate, alkyl naphthalene sulfonate, naphthalene sulfonate, diphenyl ether sulfonate, alkyl naphthalene sulfonate condensate, and naphthalene sulfonic acid condensation. Examples include physical salts.

  Examples of sulfate surfactants include alkyl sulfates, polyoxyalkylene alkyl sulfates, polyoxyalkylene alkyl phenyl ether sulfates, tristyrenated phenol sulfates, polyoxyalkylene distyrenated phenol sulfates. Etc.

Examples of the phosphate ester surfactant include alkyl phosphate ester salts, alkylphenyl phosphate ester salts, polyoxyalkylene alkyl phosphate ester salts, and polyoxyalkylene alkylphenyl phosphate ester salts.
Examples of the salt include metal salts (Na, K, Ca, Mg, Zn, etc.), ammonium salts, alkanolamine salts, aliphatic amine salts, and the like.

  Examples of amphoteric surfactants include amino acids, imidazolines, and amine oxides.

  Examples of the amino acid system include acyl amino acid salts, acyl sarcosine salts, acyloylmethylaminopropionates, alkylaminopropionates, acylamidoethylhydroxyethylmethylcarboxylates, and the like.

  Examples of amine oxides include alkyldimethylamine oxide, alkyldiethanolamine oxide, alkylamidopropylamine oxide, and the like.

  The concentration of the surfactant in the treatment liquid is preferably 0.1 to 10000 ppm, more preferably 1 to 5000 ppm, and still more preferably 10 to 1000 ppm as the concentration when applied to the plant body when spraying the leaves. When applying from the underground part in soil and hydroponics, 0.01 to 5000 ppm is preferable, 0.1 to 1000 ppm is more preferable, and 1 to 500 ppm is more preferable.

<Chelating agent>
In this invention, a chelating agent can be used with a compound (A) and also a compound (B)-a compound (H). By using a chelating agent, the stability of the composition prepared from the compound (A) and other components and the treatment liquid can be dramatically improved, and as a result, the stress tolerance imparting effect can be stabilized. When an organic acid or a salt thereof having the following chelating ability as a chelating agent is used in combination, the stress tolerance imparting effect is stabilized. Specifically, citric acid, gluconic acid, malic acid, heptonic acid, oxalic acid, malonic acid, lactic acid, tartaric acid, succinic acid, fumaric acid, maleic acid, adipic acid, glutaric acid and other oxycarboxylic acids, polyvalent carboxylic acids And potassium salts, sodium salts, alkanolamine salts, aliphatic amine salts and the like thereof. Further, a chelating agent other than an organic acid may be mixed. As a chelating agent to be mixed, ethylenediaminetetraacetic acid (EDTA) or a salt thereof, nitrilotriacetic acid (NTA) or a salt thereof, 1,2-cyclohexanediaminetetraacetic acid monohydrate. Examples thereof include aminocarboxylic acid-based chelating agents such as (CDTA) or a salt thereof.

  The concentration of the chelating agent in the treatment solution is preferably 0.1 to 10000 ppm, more preferably 1 to 5000 ppm, and even more preferably 10 to 1000 ppm when spraying the leaves as the concentration when applied to the plant body. When applying from the underground part in soil and hydroponics, 0.1-10000 ppm is preferable, 1-5000 ppm is more preferable, and 10-1000 ppm is more preferable.

<Fertilizer ingredients>
In this invention, a fertilizer component can further be used with a compound (A) and also a compound (B)-a compound (H).

As a fertilizer component, specifically, N, P, K, Ca, Mg, S, B, Fe, Mn, Cu, Zn, Mo, Cl, Si, Na, etc., and further, N, P, K, Ca, Examples include inorganic substances and organic substances that serve as a source of Mg. Examples of such an inorganic substance include ammonium nitrate, potassium nitrate, ammonium sulfate, ammonium chloride, ammonium phosphate, sodium nitrate, urea, ammonium carbonate, potassium phosphate, superphosphate lime, and molten phosphorus fertilizer (3MgO · CaO · P ₂ O ₅ · 3CaSiO _2), potassium sulfate, salts potassium nitrate of lime, slaked lime, lime carbonate, magnesium sulfate, magnesium hydroxide, magnesium carbonate, and the like. Organic substances include chicken dung, beef dung, bark compost, peptone, Mieki, fermented extract, calcium salts of organic acids (citric acid, gluconic acid, succinic acid, etc.), fatty acids (formic acid, acetic acid, propionic acid, caprylic acid) , Capric acid, caproic acid, etc.). These fertilizer components can also be used in combination with a surfactant. It is not necessary to add the fertilizer component when the fertilizer component is sufficiently applied as the original fertilizer in the soil, such as in the field cultivation of leafy vegetables. Moreover, it is preferable to mix | blend a fertilizer component with the type of cultivation form which avoids excessive application of original fertilizer and gives a fertilizer component in the same way as irrigation like a hydroponics and hydroponics.

  The concentration of the fertilizer component in the treatment liquid is preferably 0.1 to 5000 ppm, more preferably 1 to 1000 ppm for the N component, the P component, and the K component, respectively, as the concentration when applied to the plant body when spraying the leaves. Further, 10 to 500 ppm is more preferable. When applied from the underground in soil and hydroponics, the N component, P component, and K component are each preferably 0.1 to 5000 ppm, more preferably 1 to 1000 ppm, and even more preferably 10 to 500 ppm. Moreover, the density | concentration which added all the fertilizer components has preferable 1-10000 ppm, 10-5000 ppm is more preferable, and 50-2000 ppm is more preferable when foliar spraying. The concentration of all fertilizer components added is preferably 1 to 10000 ppm, more preferably 10 to 5000 ppm, and even more preferably 50 to 2000 ppm when applied from the underground in soil and hydroponics.

  Examples of plants that can impart stress tolerance according to the present invention include cucumbers, pumpkins, watermelons, melons, tomatoes, eggplants, peppers, strawberries, okras, green beans, broad beans, peas, green beans, corn, and the like. For leafy vegetables, Chinese cabbage, hornbill, cabbage, cauliflower, broccoli, cabbage, onion, leeks, garlic, rakkyo, leek, asparagus, lettuce, saladna, celery, spinach, garlic, parsley, honey bee, seri, udo, myoga , Fuki, perilla etc. Root vegetables include radish, turnip, burdock, carrot, potato, taro, sweet potato, yam, ginger, lotus root and the like. In addition, it can also be used for rice, wheat, and flowering plants.

Example 1 Salt stress tolerance test (tomato)
Test method Test conditions: temperature 23 ° C., relative humidity 50%, illuminance: 5000 Lux (fluorescent lamp), light / dark cycle: 16 hr / 8 hr
Hydroponic liquid conditions: Otsuka 1 / 2A prescription [Otsuka House 1 (N: P: K = 10: 8: 27) 7.5 g / 10 L, Otsuka House 2 (N: P: K: Ca = 10: 0 : 0:23) 5 g / 10 L compound liquid, total nitrogen 130 ppm, phosphoric acid 60 ppm, potassium 203 ppm]
Salt stress condition: NaCl concentration 3510 ppm (water potential by NaCl 0.29 MPa)
Cultivation period: 2 weeks Plant preparation: Kureha Horticultural cultivation soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / cultured soil 1 kg) manufactured by Kureha Chemical Co., Ltd. 50 holes Pack in a cell tray, seed seeds of tomato "Momotaro", cover the Kureha Horticultural soil thinly, irrigate with sufficient water and germinate. When the leaves of the second leaf stage were fully developed, the soil at the root of the tomato was carefully washed away with running water and subjected to the test.
Reagents:
Stearyl alcohol, lauryl alcohol, behenyl alcohol: Calcoal 8098, 2098, 220-80) [Kao Corporation]
POE (20) sorbitan fatty acid ester: Rheodor TW-O120V [Kao Corporation]
Alkyl polyglycoside: AG-10LK [Kao Corporation]
Sucrose fatty acid ester: Ryoto sugar ester S-1570 [Mitsubishi Chemical Foods, Inc.]
Octenyl Succinic Oxidized Starch: Emulster [Matsutani Chemical Co., Ltd.]
Silicone oil: BY22-007 [Toray Dow Corning Co., Ltd.]
Silicone surfactant: SILWET L-77 (polyoxyethylene methyl polysiloxane) [GE Toshiba Silicone Co., Ltd.]
Alkylbetaine (carbon number 12-14): Anhitoal 24B [Kao Corporation]
Other drugs: Wako Pure Chemical Industries, Ltd.
Treatment liquid application rate: foliar spray 10ml / strain Hydroponics (underground treatment) 250ml / strain

<Salt stress tolerance test method>
Environmental conditions were adjusted in an artificial weather device at a temperature of 23 ° C., an illuminance of 5000 Lux with a fluorescent lamp, a light / dark cycle of 16 hours for a light period of 16 hours, and a dark period of 8 hours. Tomatoes prepared as described above were planted in 250 ml of polyethylene bottles containing the above-mentioned hydroponic solution (1/2 Otsuka A prescription and NaCl of 3510 ppm). A treatment liquid containing the compounds shown in Tables 1 to 3 at a predetermined concentration (the balance being water) was prepared and applied to the foliage or underground. In addition, as a control group, no control stress is applied without adding NaCl (a control group for plant weight 2 of formula (ii), which is a control group without salt stress) and salt stress due to the addition of NaCl. And a control group [the control group for the plant weight 1 of the formula (ii) and a control group with salt stress] was prepared. In each of the test group and the control group, 10 individuals are prepared, and the average value of the plant raw weight of each individual two weeks after the start of the test is used as the plant raw weight, and the plant stress rate according to the above formulas (ii) and (iii) And the plant stress tolerance imparting rate was calculated. In this test condition, the plant stress rate is 130%, and it is evaluated that the stress is generated. However, the plant stress resistance imparting rate for NaCl is compared with the comparative product as shown in Tables 1 to 3 according to the present invention. The goods were clearly higher.

Example 2 Temperature stress tolerance test (tomato)
Test method Non-temperature stress test conditions: Temperature 23 ° C. Other cultivation conditions conform to the temperature stress test conditions.
Temperature stress test conditions: temperature 32 ° C., relative humidity 50%, illuminance: 5000 Lux (fluorescent lamp), light / dark cycle: 16 hr / 8 hr
Hydroponic liquid conditions: Otsuka 1 / 2A prescription [Otsuka House 1 (N: P: K = 10: 8: 27) 7.5 g / 10 L, Otsuka House 2 (N: P: K: Ca = 10: 0 : 0:23) 5 g / 10 L compound liquid, total nitrogen 130 ppm, phosphoric acid 60 ppm, potassium 203 ppm]
Cultivation period: 2 weeks Plant preparation: Kureha Horticultural cultivation soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / cultured soil 1 kg) manufactured by Kureha Chemical Co., Ltd. 50 holes Packed in a cell tray, seeded with tomato "Momotaro" seeds, thinly covered Kureha Horticultural soil, irrigated with sufficient water to germinate. When the leaves of the second leaf stage were fully developed, the soil at the root of the tomato was carefully washed away with running water and subjected to the test.
The applied chemicals and treatment liquid are applied in the same manner as in Example 1.

<Temperature stress tolerance test method>
Environmental conditions were adjusted in an artificial meteorological device at a temperature of 32 ° C., an illuminance of 5000 Lux with a fluorescent lamp, and a light / dark cycle of 16 hr light period and 8 hr dark period. In this test, a temperature of 23 ° C. at which growth was favorable was set as a reference (non-temperature stress test condition) temperature, and stress was applied by increasing the temperature from that temperature. The tomato prepared above was planted in 250 ml of a polyethylene bottle containing the hydroponic solution (1/2 Otsuka A formulation). The treatment liquids of Tables 1 to 3 were applied to the foliar surface or treated underground. In addition, as a control group, the temperature was set to 23 ° C., and a control group that was not subjected to stress (a control group for plant weight 2 of formula (ii) and a control group without temperature stress) and a temperature of 32 ° C. Then, a control group [the control group for plant weight 1 of formula (ii) and a control group with temperature stress] to which the treatment solution was not applied was prepared. In each of the test group and the control group, 10 individuals are prepared, and the average value of the plant raw weight of each individual two weeks after the start of the test is used as the plant raw weight, and the plant stress rate according to the above formulas (ii) and (iii) And the plant stress tolerance imparting rate was calculated. It should be noted that the plant stress rate is 125% under this test condition, and it is evaluated that the stress is generated. However, the plant stress resistance imparting rate with respect to the temperature is compared with the comparative product as shown in Tables 1 to 3 according to the present invention. The goods were clearly higher.

Example 3 Dry stress tolerance imparting test (tomato)
-Test method Non-drying stress test condition: Soil pF value is controlled to 1.7 or more and less than 2.3. Other cultivation conditions conform to the dry stress test condition.
Stress test condition: Soil pF value 2.3 or higher Control cultivation temperature: 23 ° C. Relative humidity 50% Illuminance: 5000 Lux (fluorescent lamp), light / dark cycle: 16 hr / 8 hr
Cultivation period: 3 weeks Soil: Kureha Horticultural cultivation soil (Kureha Chemical Co., Ltd.) (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / cultured soil 1 kg)
Preparation of plant: Kureha Horticultural culture soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / 1 kg of culture soil) manufactured by Kureha Chemical Co., Ltd. was packed in a 50-well cell tray and tomato Seed "Momotaro" seeds, cover the Kureha Horticulture soil thinly, irrigate with enough water to germinate. When the leaves of the second leaf stage were fully developed, the soil at the root of the tomato was carefully washed away with running water and subjected to the test.
The concentrations of the chemicals used and the treatment liquid are the same as in Example 1.
Treatment liquid application rate: foliar spray 10ml / soil soil irrigation 50ml / strain

<Drying stress tolerance test method>
Five strains of the tomatoes prepared above were planted in a planter of 60 cm on a plant culture shelf in a temperature-controlled room. A soil moisture meter (manufactured by Daiki Rika Kogyo Co., Ltd.) was installed in the center of the planter, and irrigation was performed several times a day while confirming the state of soil moisture. The drought stress was controlled so that the pF value was 2.3 or more. Immediately after planting the tomatoes, the treatment liquids shown in Tables 1 to 3 were applied to the foliage or treated underground at a rate of once a week. In addition, as a control plot, a control plot that does not give a stress irrigated so that the soil pF value is between 1.7 and less than 2.3 [control plot for plant weight 2 of formula (ii), and drought stress No control group] and a control group that is irrigated to give a pF value of 2.3 or more and no treatment solution is applied [control group for plant weight 1 of formula (ii), with drought stress It is a control plot]. In the test group and the control group, 5 individuals (1 planter) are prepared. After 3 weeks from the start of the test, the plant body is sampled so as not to cut the roots, the soil is washed away with running water, and the raw plant weight is measured. The plant stress rate and the plant stress tolerance imparting rate were calculated by the above formulas (ii) and (iii) as average values. It should be noted that the plant drought stress rate is 130% under this test condition, and it is evaluated that stress has occurred, but the plant stress tolerance imparting rate for drought is compared with that of the comparative product as shown in Tables 1 to 3. The invention was clearly higher.

Example 4 Salt stress tolerance imparting test (tomato)
Test method Test conditions: temperature 23 ° C., relative humidity 50%, illuminance: 5000 Lux (fluorescent lamp), light / dark cycle: 16 hr / 8 hr
Hydroponic liquid condition: tap salt stress condition: NaCl concentration 3510ppm (water potential by NaCl 0.29MPa)
Cultivation period: 2 weeks Plant preparation: Kureha Horticultural cultivation soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / cultured soil 1 kg) manufactured by Kureha Chemical Co., Ltd. 50 holes Packed in a cell tray, seeded with tomato "Momotaro" seeds, thinly covered Kureha Horticultural soil, irrigated with sufficient water to germinate. When the leaves of the second leaf stage were fully developed, the soil at the root of the tomato was carefully washed away with running water and subjected to the test.
The concentrations of the chemicals used and the treatment liquid are the same as in Example 1.
Treatment liquid application rate: foliar spray 10ml / strain underground treatment 250ml / strain

<Salt stress tolerance test method>
The test plot and the control plot were cultivated in the same manner as in Example 1 except that the hydroponic solution used was tap water with NaCl added to a concentration of 3510 ppm. As a result, the plant stress rate is 130% under this test condition, and it is evaluated that stress is occurring. However, the plant stress resistance imparting rate for NaCl is compared with that of the comparative product as shown in Tables 1 to 3. The product of the invention was clearly higher, and the plant stress resistance imparting rate was 111% or more.

Example 5 Dry stress tolerance imparting test (potato: variety Toyoshiro)
-Test method Non-drying stress test condition: Control to soil pF value 1.7-2.3 Other cultivation conditions conform to the dry stress test condition.
Stress test condition: Soil pF value 2.3 or higher Control cultivation temperature: 23 ° C. Relative humidity 50% Illuminance: 5000 Lux (fluorescent lamp), light / dark cycle: 16 hr / 8 hr
Cultivation period: 3 weeks Soil: Kureha Horticultural cultivation soil (Kureha Chemical Co., Ltd.) (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / cultured soil 1 kg)
The applied chemicals and the treatment liquid are applied in the same manner as in Example 3.

<Drying stress tolerance test method>
In the glass greenhouse, Kureha Horticultural Culture Soil (fertilizer component; N: P: K = 0.4: 1.9: 0.6 (g) / 1 kg of culture soil) made by Kureha Chemical Co., Ltd. was packed into a 60 cm planter and potato “Toyoshiro” seed pods were cut in half and sown 5 pieces at a time, and Kureha horticulture soil was thinly covered, and water was thoroughly sprinkled to allow germination. At the stage where the leaves at the 3 leaf stage were fully developed, the potato was moved to a plant culture shelf in a room where the temperature was controlled, and the test was started. A soil moisture meter was installed in the center of the planter, and irrigation was performed once a day while confirming the state of soil moisture. The drought stress was controlled so that the pF value was 2.3 or more. Immediately after the start of the test, the treatment liquids shown in Tables 1 to 3 were applied to the foliar surface or treated underground at a rate of once a week. In addition, as a control plot, a control plot that does not give a stress irrigated so that the soil pF value is between 1.7 and less than 2.3 [control plot for plant weight 2 of formula (ii), and drought stress Control group without irrigation] and control group in which irrigation is controlled so that the pF value is 2.3 or more and no treatment solution is given [control group for plant weight 1 of formula (ii), and drought stress This is a control zone. In the test group and the control group, 5 individuals (1 planter) are prepared. After 3 weeks from the start of the test, the plant body is sampled so as not to cut the roots, the soil is washed away with running water, and the raw plant weight is measured. The plant stress rate and the plant stress tolerance imparting rate were calculated by the above formulas (ii) and (iii) as average values. It should be noted that the plant stress rate is 128% under the present test conditions, and it is evaluated that stress has occurred. However, the plant stress resistance imparting rate for drying is compared with the comparative products as shown in Tables 1 to 3, according to the present invention. The goods were clearly higher.

Claims (3)

_{(A) CH 3 (CH 2} ) s-1 OH (s 12 to 24 of an integer) is represented by 1-alkanol, as well (C) a silicon compound [hereinafter referred to as the compound (C)], (F) Antioxidant [hereinafter referred to as compound (F)] , (G) Auxin biosynthetic pathway intermediate substance (biosynthetic pathway from shikimic acid to indoleacetic acid) [hereinafter referred to as compound (G)] and (H) saccharide [ Hereinafter, a plant stress tolerance imparting agent composition containing one or more compounds selected from the group consisting of compounds (H)] has a water potential of 0.2 MPa or more due to salt stress and a soil pF value due to drought stress. 2.3 or more, and an average cultivation temperature due to temperature stress is 28 ° C. or more, and is applied to a plant under cultivation conditions including at least one selected from the group of the following:
Compound (C) is one or more compounds selected from the group consisting of silicon, silicic acid, orthosilicic acid, disilicic acid, trisilicic acid, polysilicic acid, silicone oil, and silicone surfactant, and compound (F) is flavone, quercetin , Rutin, chlorophyll, saponin, caffeine, catechin, polyphenol, sesamin, sesamol, astaxanthin, and curcumin, wherein compound (G) is shikimic acid, anthranilic acid, tryptamine, and indoleacetic acid One or more compounds selected from the group consisting of: Compound (H) is one or more compounds selected from the group consisting of trehalose, raffinose, fructose, glucosyl sucrose, cyclodextrin, mannitol and oligosaccharides;
The plant is tomato or potato,
Plant cultivation method . Furthermore, surfactants cultivation method of the plant according to claim 1, wherein applying the [(A), (C), (F), (G), belonging excluding those in (H)]. The plant cultivation method according to claim 1 or 2, wherein the surfactant is alkenyl succinylated starch.