JP5021183B2 - タンパク質固定化膜および固定化方法、ならびにバイオセンサ - Google Patents
タンパク質固定化膜および固定化方法、ならびにバイオセンサ Download PDFInfo
- Publication number
- JP5021183B2 JP5021183B2 JP2005148253A JP2005148253A JP5021183B2 JP 5021183 B2 JP5021183 B2 JP 5021183B2 JP 2005148253 A JP2005148253 A JP 2005148253A JP 2005148253 A JP2005148253 A JP 2005148253A JP 5021183 B2 JP5021183 B2 JP 5021183B2
- Authority
- JP
- Japan
- Prior art keywords
- cygdh
- immobilized
- cell membrane
- biosensor
- layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims description 28
- 108090000623 proteins and genes Proteins 0.000 title claims description 7
- 102000004169 proteins and genes Human genes 0.000 title claims description 6
- 239000012528 membrane Substances 0.000 title claims description 5
- 229920000642 polymer Polymers 0.000 claims description 43
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 42
- 239000008103 glucose Substances 0.000 claims description 42
- 150000003904 phospholipids Chemical class 0.000 claims description 32
- 210000004027 cell Anatomy 0.000 claims description 31
- 102000018832 Cytochromes Human genes 0.000 claims description 20
- 108010052832 Cytochromes Proteins 0.000 claims description 20
- 230000027756 respiratory electron transport chain Effects 0.000 claims description 19
- 230000000694 effects Effects 0.000 claims description 17
- ZSZRUEAFVQITHH-UHFFFAOYSA-N 2-(2-methylprop-2-enoyloxy)ethyl 2-(trimethylazaniumyl)ethyl phosphate Chemical group CC(=C)C(=O)OCCOP([O-])(=O)OCC[N+](C)(C)C ZSZRUEAFVQITHH-UHFFFAOYSA-N 0.000 claims description 12
- 230000003100 immobilizing effect Effects 0.000 claims description 10
- 238000011282 treatment Methods 0.000 claims description 10
- 239000006087 Silane Coupling Agent Substances 0.000 claims description 8
- 238000006356 dehydrogenation reaction Methods 0.000 claims description 8
- 210000002390 cell membrane structure Anatomy 0.000 claims description 6
- 239000010410 layer Substances 0.000 description 90
- 102000004316 Oxidoreductases Human genes 0.000 description 31
- 108090000854 Oxidoreductases Proteins 0.000 description 31
- 239000008280 blood Substances 0.000 description 21
- 210000004369 blood Anatomy 0.000 description 21
- 239000003153 chemical reaction reagent Substances 0.000 description 21
- 239000000758 substrate Substances 0.000 description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 20
- 229910052799 carbon Inorganic materials 0.000 description 20
- 230000004044 response Effects 0.000 description 19
- 238000005259 measurement Methods 0.000 description 11
- 239000000463 material Substances 0.000 description 9
- 210000000170 cell membrane Anatomy 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 238000000089 atomic force micrograph Methods 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 230000004043 responsiveness Effects 0.000 description 6
- 125000006850 spacer group Chemical group 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000589513 Burkholderia cepacia Species 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000002848 electrochemical method Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000004040 coloring Methods 0.000 description 3
- 150000004696 coordination complex Chemical class 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000007650 screen-printing Methods 0.000 description 3
- 239000002344 surface layer Substances 0.000 description 3
- SOGAXMICEFXMKE-UHFFFAOYSA-N Butylmethacrylate Chemical compound CCCCOC(=O)C(C)=C SOGAXMICEFXMKE-UHFFFAOYSA-N 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004502 linear sweep voltammetry Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 108091005703 transmembrane proteins Proteins 0.000 description 2
- 102000035160 transmembrane proteins Human genes 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
- OXYZDRAJMHGSMW-UHFFFAOYSA-N 3-chloropropyl(trimethoxy)silane Chemical compound CO[Si](OC)(OC)CCCCl OXYZDRAJMHGSMW-UHFFFAOYSA-N 0.000 description 1
- UUEWCQRISZBELL-UHFFFAOYSA-N 3-trimethoxysilylpropane-1-thiol Chemical compound CO[Si](OC)(OC)CCCS UUEWCQRISZBELL-UHFFFAOYSA-N 0.000 description 1
- XDLMVUHYZWKMMD-UHFFFAOYSA-N 3-trimethoxysilylpropyl 2-methylprop-2-enoate Chemical compound CO[Si](OC)(OC)CCCOC(=O)C(C)=C XDLMVUHYZWKMMD-UHFFFAOYSA-N 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- 229920002978 Vinylon Polymers 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000009118 appropriate response Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 208000028659 discharge Diseases 0.000 description 1
- WOXXJEVNDJOOLV-UHFFFAOYSA-N ethenyl-tris(2-methoxyethoxy)silane Chemical compound COCCO[Si](OCCOC)(OCCOC)C=C WOXXJEVNDJOOLV-UHFFFAOYSA-N 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 125000005397 methacrylic acid ester group Chemical group 0.000 description 1
- -1 monosodium salt Chemical class 0.000 description 1
- KBJFYLLAMSZSOG-UHFFFAOYSA-N n-(3-trimethoxysilylpropyl)aniline Chemical compound CO[Si](OC)(OC)CCCNC1=CC=CC=C1 KBJFYLLAMSZSOG-UHFFFAOYSA-N 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
- 238000004528 spin coating Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 229920005992 thermoplastic resin Polymers 0.000 description 1
- GQIUQDDJKHLHTB-UHFFFAOYSA-N trichloro(ethenyl)silane Chemical compound Cl[Si](Cl)(Cl)C=C GQIUQDDJKHLHTB-UHFFFAOYSA-N 0.000 description 1
- JXUKBNICSRJFAP-UHFFFAOYSA-N triethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical compound CCO[Si](OCC)(OCC)CCCOCC1CO1 JXUKBNICSRJFAP-UHFFFAOYSA-N 0.000 description 1
- QQQSFSZALRVCSZ-UHFFFAOYSA-N triethoxysilane Chemical compound CCO[SiH](OCC)OCC QQQSFSZALRVCSZ-UHFFFAOYSA-N 0.000 description 1
- DQZNLOXENNXVAD-UHFFFAOYSA-N trimethoxy-[2-(7-oxabicyclo[4.1.0]heptan-4-yl)ethyl]silane Chemical compound C1C(CC[Si](OC)(OC)OC)CCC2OC21 DQZNLOXENNXVAD-UHFFFAOYSA-N 0.000 description 1
- 238000007740 vapor deposition Methods 0.000 description 1
- 239000005050 vinyl trichlorosilane Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/80—Cytochromes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/80—Cytochromes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Emergency Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005148253A JP5021183B2 (ja) | 2005-05-20 | 2005-05-20 | タンパク質固定化膜および固定化方法、ならびにバイオセンサ |
| EP06746594.8A EP1884771B1 (en) | 2005-05-20 | 2006-05-18 | Protein-immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor |
| US11/920,782 US20090101499A1 (en) | 2005-05-20 | 2006-05-18 | Protein-Immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor |
| PCT/JP2006/309906 WO2006123730A1 (ja) | 2005-05-20 | 2006-05-18 | タンパク質固定膜、タンパク質の固定化方法、酵素固定化電極、およびバイオセンサ |
| CN2006800226213A CN101203748B (zh) | 2005-05-20 | 2006-05-18 | 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 |
| US14/850,576 US9702843B2 (en) | 2005-05-20 | 2015-09-10 | Biosensor incorporating protein-immobilized membrane and method of immobilizing protein in biosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005148253A JP5021183B2 (ja) | 2005-05-20 | 2005-05-20 | タンパク質固定化膜および固定化方法、ならびにバイオセンサ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006322889A JP2006322889A (ja) | 2006-11-30 |
| JP2006322889A5 JP2006322889A5 (enExample) | 2008-03-06 |
| JP5021183B2 true JP5021183B2 (ja) | 2012-09-05 |
Family
ID=37431302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005148253A Expired - Fee Related JP5021183B2 (ja) | 2005-05-20 | 2005-05-20 | タンパク質固定化膜および固定化方法、ならびにバイオセンサ |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20090101499A1 (enExample) |
| EP (1) | EP1884771B1 (enExample) |
| JP (1) | JP5021183B2 (enExample) |
| CN (1) | CN101203748B (enExample) |
| WO (1) | WO2006123730A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060091006A1 (en) * | 1999-11-04 | 2006-05-04 | Yi Wang | Analyte sensor with insertion monitor, and methods |
| EP1661516B1 (en) * | 2003-09-02 | 2012-08-15 | Koji Sode | Glucose sensor and glucose level measuring apparatus |
| JP2008243380A (ja) * | 2007-03-23 | 2008-10-09 | Sony Corp | 酵素固定化電極、燃料電池、電子機器、酵素反応利用装置および酵素固定化基体 |
| US8268604B2 (en) * | 2007-12-20 | 2012-09-18 | Abbott Point Of Care Inc. | Compositions for forming immobilized biological layers for sensing |
| US9653006B2 (en) | 2008-09-17 | 2017-05-16 | Avery Dennison Corporation | Activatable adhesive, labels, and related methods |
| WO2010090271A1 (ja) | 2009-02-09 | 2010-08-12 | アークレイ株式会社 | 電気化学センサーおよびその作製方法 |
| JP5432575B2 (ja) * | 2009-04-21 | 2014-03-05 | グンゼ株式会社 | バイオセンサ及びその製造方法 |
| WO2010126139A1 (ja) | 2009-04-30 | 2010-11-04 | 池田食研株式会社 | 蛋白質性電子メディエータ |
| CN102449086B (zh) | 2009-09-17 | 2014-08-13 | 艾利丹尼森公司 | 可活化粘合剂、标签及相关方法 |
| JP5665070B2 (ja) * | 2009-09-25 | 2015-02-04 | 独立行政法人産業技術総合研究所 | 酸化還元タンパク質固定化ナノ構造電極 |
| CN103344639B (zh) * | 2013-07-11 | 2015-10-28 | 山东大学 | 一种快速检测尿中吡咯加合物的检测管 |
| CN105492902B (zh) * | 2013-08-07 | 2020-07-24 | 爱科来株式会社 | 使用电化学式生物传感器的物质测量方法和测量装置 |
| EP3305721B1 (en) | 2015-06-08 | 2023-10-18 | Japan Science And Technology Agency | High-density micro-chamber array and measurement method using same |
| CN105355530B (zh) * | 2015-10-30 | 2017-08-11 | 三诺生物传感股份有限公司 | 一种用于试条电极预处理的等离子处理装置和方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0810208A (ja) | 1994-06-30 | 1996-01-16 | Toshiba Corp | 食器洗浄機 |
| US5650062A (en) * | 1995-03-17 | 1997-07-22 | Matsushita Electric Industrial Co., Ltd. | Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same |
| CN1321245A (zh) * | 1999-09-13 | 2001-11-07 | 松下电器产业株式会社 | 脂质修饰酶的制备方法及生物传感器 |
| JP2003525320A (ja) * | 2000-02-18 | 2003-08-26 | アスピラ バイオシステムズ，インコーポレイティド | 分子インプリンティング組成物、並びに高分子の結合及び分析方法 |
| US6458599B1 (en) | 2000-02-18 | 2002-10-01 | Aspira Biosystems, Inc. | Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules |
| JP2004512062A (ja) * | 2000-07-28 | 2004-04-22 | エモリー ユニバーシテイ | 人工膜から成る生物学的構成要素 |
| JP2002055076A (ja) * | 2000-09-08 | 2002-02-20 | Nec Corp | 電気化学センサ |
| WO2002036779A1 (fr) * | 2000-10-31 | 2002-05-10 | Koji Sode | Nouvelle glucose deshydrogenase et procede de production de la deshydrogenase |
| JP4385219B2 (ja) * | 2001-09-14 | 2009-12-16 | アークレイ株式会社 | 濃度測定方法 |
| EP1522592B1 (en) * | 2002-06-17 | 2008-08-06 | ARKRAY, Inc. | Glucose sensor with the use of glucose dehydrogenase |
| CN1672280A (zh) * | 2002-07-25 | 2005-09-21 | 松下电器产业株式会社 | 电解质膜和使用其的膜电极接合体以及燃料电池 |
| JP4250481B2 (ja) * | 2003-08-11 | 2009-04-08 | キヤノン株式会社 | 多孔質構造体 |
| EP1661516B1 (en) * | 2003-09-02 | 2012-08-15 | Koji Sode | Glucose sensor and glucose level measuring apparatus |
| WO2005030807A1 (ja) * | 2003-09-30 | 2005-04-07 | Koji Sode | グルコース脱水素酵素とシトクロームとの融合蛋白質 |
-
2005
- 2005-05-20 JP JP2005148253A patent/JP5021183B2/ja not_active Expired - Fee Related
-
2006
- 2006-05-18 EP EP06746594.8A patent/EP1884771B1/en not_active Not-in-force
- 2006-05-18 CN CN2006800226213A patent/CN101203748B/zh not_active Expired - Fee Related
- 2006-05-18 US US11/920,782 patent/US20090101499A1/en not_active Abandoned
- 2006-05-18 WO PCT/JP2006/309906 patent/WO2006123730A1/ja not_active Ceased
-
2015
- 2015-09-10 US US14/850,576 patent/US9702843B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US9702843B2 (en) | 2017-07-11 |
| EP1884771B1 (en) | 2017-12-13 |
| EP1884771A4 (en) | 2011-10-19 |
| CN101203748B (zh) | 2011-09-14 |
| WO2006123730A1 (ja) | 2006-11-23 |
| CN101203748A (zh) | 2008-06-18 |
| JP2006322889A (ja) | 2006-11-30 |
| US20090101499A1 (en) | 2009-04-23 |
| EP1884771A1 (en) | 2008-02-06 |
| US20150377818A1 (en) | 2015-12-31 |
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