CN101203748B - 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 - Google Patents

蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 Download PDF

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CN101203748B
CN101203748B CN2006800226213A CN200680022621A CN101203748B CN 101203748 B CN101203748 B CN 101203748B CN 2006800226213 A CN2006800226213 A CN 2006800226213A CN 200680022621 A CN200680022621 A CN 200680022621A CN 101203748 B CN101203748 B CN 101203748B
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enzyme
immobilized
biosensor
cell membrane
layer
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CN101203748A (zh
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胜木幸治
山冈秀亮
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Arkray Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/80—Cytochromes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Emergency Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2006800226213A 2005-05-20 2006-05-18 蛋白质固定膜、蛋白质的固定化方法、酶固定化电极和生物传感器 Expired - Fee Related CN101203748B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2005148253A JP5021183B2 (ja) 2005-05-20 2005-05-20 タンパク質固定化膜および固定化方法、ならびにバイオセンサ
JP148253/2005 2005-05-20
PCT/JP2006/309906 WO2006123730A1 (ja) 2005-05-20 2006-05-18 タンパク質固定膜、タンパク質の固定化方法、酵素固定化電極、およびバイオセンサ

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CN101203748A CN101203748A (zh) 2008-06-18
CN101203748B true CN101203748B (zh) 2011-09-14

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US (2) US20090101499A1 (enExample)
EP (1) EP1884771B1 (enExample)
JP (1) JP5021183B2 (enExample)
CN (1) CN101203748B (enExample)
WO (1) WO2006123730A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103344639A (zh) * 2013-07-11 2013-10-09 山东大学 一种快速检测尿中吡咯加合物的检测管

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US20060091006A1 (en) * 1999-11-04 2006-05-04 Yi Wang Analyte sensor with insertion monitor, and methods
EP1661516B1 (en) * 2003-09-02 2012-08-15 Koji Sode Glucose sensor and glucose level measuring apparatus
JP2008243380A (ja) * 2007-03-23 2008-10-09 Sony Corp 酵素固定化電極、燃料電池、電子機器、酵素反応利用装置および酵素固定化基体
US8268604B2 (en) * 2007-12-20 2012-09-18 Abbott Point Of Care Inc. Compositions for forming immobilized biological layers for sensing
US9653006B2 (en) 2008-09-17 2017-05-16 Avery Dennison Corporation Activatable adhesive, labels, and related methods
WO2010090271A1 (ja) 2009-02-09 2010-08-12 アークレイ株式会社 電気化学センサーおよびその作製方法
JP5432575B2 (ja) * 2009-04-21 2014-03-05 グンゼ株式会社 バイオセンサ及びその製造方法
WO2010126139A1 (ja) 2009-04-30 2010-11-04 池田食研株式会社 蛋白質性電子メディエータ
CN102449086B (zh) 2009-09-17 2014-08-13 艾利丹尼森公司 可活化粘合剂、标签及相关方法
JP5665070B2 (ja) * 2009-09-25 2015-02-04 独立行政法人産業技術総合研究所 酸化還元タンパク質固定化ナノ構造電極
CN105492902B (zh) * 2013-08-07 2020-07-24 爱科来株式会社 使用电化学式生物传感器的物质测量方法和测量装置
EP3305721B1 (en) 2015-06-08 2023-10-18 Japan Science And Technology Agency High-density micro-chamber array and measurement method using same
CN105355530B (zh) * 2015-10-30 2017-08-11 三诺生物传感股份有限公司 一种用于试条电极预处理的等离子处理装置和方法

Citations (2)

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CN1321245A (zh) * 1999-09-13 2001-11-07 松下电器产业株式会社 脂质修饰酶的制备方法及生物传感器
US6773563B2 (en) * 2000-08-09 2004-08-10 Nec Corporation Electrochemical sensor having a reference electrode

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JPH0810208A (ja) 1994-06-30 1996-01-16 Toshiba Corp 食器洗浄機
US5650062A (en) * 1995-03-17 1997-07-22 Matsushita Electric Industrial Co., Ltd. Biosensor, and a method and a device for quantifying a substrate in a sample liquid using the same
JP2003525320A (ja) * 2000-02-18 2003-08-26 アスピラ バイオシステムズ，インコーポレイティド 分子インプリンティング組成物、並びに高分子の結合及び分析方法
US6458599B1 (en) 2000-02-18 2002-10-01 Aspira Biosystems, Inc. Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules
JP2004512062A (ja) * 2000-07-28 2004-04-22 エモリー　ユニバーシテイ 人工膜から成る生物学的構成要素
WO2002036779A1 (fr) * 2000-10-31 2002-05-10 Koji Sode Nouvelle glucose deshydrogenase et procede de production de la deshydrogenase
JP4385219B2 (ja) * 2001-09-14 2009-12-16 アークレイ株式会社 濃度測定方法
EP1522592B1 (en) * 2002-06-17 2008-08-06 ARKRAY, Inc. Glucose sensor with the use of glucose dehydrogenase
CN1672280A (zh) * 2002-07-25 2005-09-21 松下电器产业株式会社 电解质膜和使用其的膜电极接合体以及燃料电池
JP4250481B2 (ja) * 2003-08-11 2009-04-08 キヤノン株式会社 多孔質構造体
EP1661516B1 (en) * 2003-09-02 2012-08-15 Koji Sode Glucose sensor and glucose level measuring apparatus
WO2005030807A1 (ja) * 2003-09-30 2005-04-07 Koji Sode グルコース脱水素酵素とシトクロームとの融合蛋白質

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CN1321245A (zh) * 1999-09-13 2001-11-07 松下电器产业株式会社 脂质修饰酶的制备方法及生物传感器
US6773563B2 (en) * 2000-08-09 2004-08-10 Nec Corporation Electrochemical sensor having a reference electrode

Non-Patent Citations (1)

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Z. Salamon and G.Tollin.Interfacial electrochemistry of cytochrome c at a lipid bilayer modified electrode:effect of incorporation of negative charges into the bilayer on cyclic voltammetric parameters.《Bioelectrochemistry and Bioenergetics》.1991,第26卷(第2期),321-334. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103344639A (zh) * 2013-07-11 2013-10-09 山东大学 一种快速检测尿中吡咯加合物的检测管
CN103344639B (zh) * 2013-07-11 2015-10-28 山东大学 一种快速检测尿中吡咯加合物的检测管

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Publication number Publication date
US9702843B2 (en) 2017-07-11
EP1884771B1 (en) 2017-12-13
EP1884771A4 (en) 2011-10-19
JP5021183B2 (ja) 2012-09-05
WO2006123730A1 (ja) 2006-11-23
CN101203748A (zh) 2008-06-18
JP2006322889A (ja) 2006-11-30
US20090101499A1 (en) 2009-04-23
EP1884771A1 (en) 2008-02-06
US20150377818A1 (en) 2015-12-31

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