JP4691320B2 - Composition for inhibiting fat accumulation - Google Patents

Description

  The present invention relates to a composition for inhibiting fat accumulation, and particularly to a composition for inhibiting fat accumulation comprising essential oil.

  Obesity is a hotbed for various lifestyle-related diseases, and improvement of obesity is important for maintaining health. There are many people who are obese in modern society with excessive caloric intake and lack of exercise. In Japan, there are about 13 million men and 10 million women who exceed BMI (physique index) 25, which is the standard for obesity. It is said that providing an obesity improvement method has become an important issue. The cause of obesity is that excessive calories ingested are stored in adipose tissue (adipocytes) as neutral fat.

For the purpose of developing a drug having a slimming effect, screening of various herbal medicines, extracts, drugs and the like showing effects such as suppression of fat synthesis and promotion of lipolysis is performed. For example, it has already been reported that caffeine reduces excess fat accumulation, and capsicum ethanol extract reduces the amount of triglyceride fat by an action that seems to be due to inhibition of differentiation and maturation.
On the other hand, essential oils obtained by steam-distilling plants have conventionally been known for various pharmacological actions such as bactericidal action, anti-inflammatory action, and sedative action.
JP 08-169896 A Keiko Iwashita, Eisuke Munakata, Toujiro Tsushida: 'Study on cell function control of animal cultured cells 3T3-L1 by food components' Grant-in-Aid for Research on Food No.12p163-168 (1999).

However, there have been few reports on researches that systematically investigated the effect of suppressing fat accumulation, the effect of improving obesity, particularly the direct action on adipose tissue (adipocytes).
Searching for essential oils that act on adipose tissue to prevent slimming and obesity, and providing skin preparations, clothing, foods, etc. that contain these essential oils improve obesity and maintain people's health It is an important issue above, and its industrial utility value is also great.
The present invention has been made in view of the above-described problems, and an object of the present invention is to provide a fat accumulation-suppressing composition capable of suppressing fat accumulation by directly acting on adipose tissue. It is in.

As a result of studies to solve the above problems, the present inventors have found that a specific essential oil has an effect of directly acting on adipose tissue and suppressing the accumulation of fat in fat cells, thereby completing the present invention. .
That is, the first subject of the present invention is eucalyptus oil, laurel oil, grapefruit oil, rosemary oil, clovebud oil, chamomile oil, rose oil, geranium oil, neroli oil, pine needle oil, hyssop oil, fennel oil, A fat accumulation-suppressing composition comprising one or more essential oils selected from the group consisting of clary sage oil and galvanum oil.

The second subject of the present invention is an external preparation for skin, a slimming cosmetic, an obesity-improving agent, clothing and food, characterized by comprising the above composition.
The third subject of the present invention is a method for inhibiting fat accumulation, characterized in that the composition is applied to the skin.

  According to the present invention, a composition having a high effect of suppressing fat accumulation can be provided.

Hereinafter, preferred embodiments of the present invention will be described.
The present invention was carried out paying attention to the fact that a specific essential oil has an effect of directly acting on adipose tissue and suppressing the accumulation of fat in fat cells, and the effect of inhibiting fat accumulation according to the present invention. Is a group consisting of Eucalyptus oil, Laurel oil, Grapefruit oil, Rosemary oil, Clovebud oil, Chamomile oil, Rose oil, Geranium oil, Neroli oil, Pine needle oil, Hyssop oil, Fennel oil, Clary sage oil, and Galvanum oil It is exhibited by including one or more essential oils selected more.

<Eucalyptus oil>
It is an essential oil obtained by steam distillation of leaves, twigs, etc. of evergreen persimmon eucalyptus belonging to Myrtaceae.
<Laurel oil>
This is an essential oil obtained by steam distillation after drying leaves of Laurus nobilis L. belonging to the genus Laurus of the family Lauraceae.

<Grapefruit oil>
Oil is obtained at the time of fruit juice production of Citrus paradis Macf.
<Rosemary oil>
It is an essential oil obtained by steam distillation of leaves and flowers of evergreen shrub Rosemary (Rosmarinus officinalis) of Lamiaceae.
<Clove Bad Oil>
It is an essential oil obtained by drying and steam-distilling flower buds before the flowering of Eugenia caryophyllata.

<Chamomile oil>
Chamomile the (Or m e n is mu l ticaul i s) is essential oil obtained by steam distillation.
<Rose oil>
Steam-distilling roses such as Damask Rose (Rosa damascena Mill.), Cabbage Rose (Rosa centifolia L.), Rosa alva L., Rosa gallica L. Is an essential oil.
<Geranium oil>
It is an essential oil obtained by harvesting the branches and leaves of the plant P. graveolens belonging to the genus Pelargonium belonging to the genus Pelargonium of the family Geraniaceae before flowering and steam distillation.

<Neroli Oil>
It is an essential oil obtained by steam distillation of the flowers of Citrus aurantium subs pamera.
<Pine needle oil>
It is an essential oil obtained by steam-distilling needles and twigs such as pine of Pinus sylvestris and Pinus sylvestris of the pine family and Abies.
<Hissop oil>
It is an essential oil obtained by steam-distilling the leaves and ears of Hyssopus officinalis L.

<Fennel oil>
It is an essential oil obtained by steam-distilling the fruits of the Aceraceae or Foeniculum vulgare Miller.
<Clary sage oil>
It is an essential oil obtained by steam-distilling flower spikes or whole plants during the flowering period of clary sage (Salvia sclarea L.).
<Galvanum oil>
It is an essential oil obtained by refining the exudates of Ferula galbanifua and related plants of the Apiaceae family.
(Please refer to “Encyclopedia of Scents” edited by Japan Fragrance Association, “Encyclopedia of Latest Fragrances” edited by Asakura Shoten)

In addition, in the range which does not impair the effect of this invention, essential oils other than the above can also be included.
The composition for suppressing fat accumulation according to the present invention can be applied to an external preparation for skin, food, feed, clothing, footwear, accessories and the like.
In the external preparation for skin of the present invention, in addition to the above-described composition for inhibiting fat accumulation, other ingredients usually used in cosmetics, quasi-drugs, pharmaceuticals, etc., as long as the effects of the present invention are not impaired, for example, powder Ingredients, liquid fats and oils, solid fats and oils, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, humectants, high water solubility Molecule, thickener, film agent, UV absorber, sequestering agent, lower alcohol, polyhydric alcohol, sugar, amino acid, organic amine, polymer emulsion, pH adjuster, skin nutrient, vitamin, antioxidant, Antioxidant auxiliaries, fragrances, water and the like can be appropriately blended as necessary, and can be produced by conventional methods according to the intended dosage form.

  Other ingredients that can be blended include, for example, preservatives (ethyl paraben, butyl paraben, etc.); anti-inflammatory agents (eg, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, zinc oxide, allantoin, etc.); Extract, placenta extract, saxifrage extract, arbutin, etc.); various extracts (eg, buckwheat, auren, shikon, peonies, assembly, birch, sage, loquat, carrot, aloe, mallow), iris, grape, yokuinin, loofah, lily , Saffron, nematode, ginger, hypericum, onionis, garlic, capsicum, chimney, red snapper, seaweed, etc.), activator (eg, royal jelly, photosensitizer, cholesterol derivative, etc.); blood circulation promoter (eg, nonyl acid wallenylamide, nicotine) Acid Gyl ester, nicotinic acid β-butoxyethyl ester, capsaicin, gingerone, cantalis tincture, ictamol, tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, cyclandrate, cinnarizine, trazoline, acetylcholine, verapamil, cephalanthin , Γ-oryzanol, etc.); antiseborrheic agents (eg, sulfur, thianthol, etc.); anti-inflammatory agents (eg, tranexamic acid, thiotaurine, hypotaurine, etc.) and the like.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, malic acid and other metal sequestering agents, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice, Various herbal extracts such as Karin and Ichiyakusou, drugs such as tocopherol acetate, glycyrrhizic acid, glycyrrhizic acid and derivatives or salts thereof, whitening agents such as vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, Amino acids such as arginine and lysine and derivatives thereof, saccharides such as fructose, mannose, erythritol, trehalose and xylitol can also be appropriately blended.

  The external preparation for skin of the present invention can be widely applied to cosmetics, pharmaceuticals, and quasi drugs applied to the outer skin. The dosage form is arbitrary, such as solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, gel, aerosol, mist, capsule, etc. It can be provided in the form. In addition, the product form of the external preparation for skin of the present invention is also arbitrary, body cosmetics such as massage agents and foot sprays; bath preparations; facial cosmetics such as lotions, emulsions, creams, packs, etc .; foundations, funny, blushers, Makeup cosmetics such as lipstick, eye shadow, eyeliner, mascara and sunscreen; aromatic cosmetics; skin cleansing agents such as make-up removers, facial cleansers, body shampoos; hair cosmetics such as hair rinses and shampoos; ointments; It can be applied in any form as long as it is used for conventional skin external preparations such as paper.

The blending amount of the essential oil in the external preparation for skin is not particularly limited, but is preferably 0.001 to 50% by mass, more preferably 0.002 to 30% by mass based on the entire composition. If it is less than 0.001% by mass, a sufficient fat accumulation inhibitory effect may not be observed, and even if it exceeds 50% by mass, a further increase in fat accumulation inhibitory effect is hardly observed.
As food, it can be applied to bread, rice cakes, noodles, dairy products, edible fats and oils, confectionery, jam, honey, egg products, beverages and the like.
As clothing, it can be applied to underwear, socks, stockings, girdle, nightwear and the like.

Hereinafter, preferred embodiments of the present invention will be described in more detail. In addition, this invention is not limited by this. Further, the blending amount (%) is mass% unless otherwise specified.
Fat accumulation inhibitory effect test The present inventors have studied eucalyptus oil, laurel oil, grapefruit oil, rosemary oil, clovebad oil, chamomile oil, rose oil, geranium oil in order to investigate the effect of essential oils to suppress fat accumulation. The following tests were carried out using 14 kinds of essential oils, neroli oil, pine needle oil, hyssop oil, fennel oil, clary sage oil, and galvanum oil.

<Adipocyte culture method>
Rat subcutaneous fat-derived preadipocytes (Toyobo) were seeded in 24-well plates at a density of 2-3 × 10 ⁴ cells / well, and 37 ° C. in DMEM medium containing 10% fetal calf serum until confluent. Growth culture was performed under conditions of 5% CO ₂ .
After reaching confluence, various essential oils diluted with ethanol were replaced with a differentiation medium added to a final concentration of 0.01% in the medium, and differentiation culture was performed. As the differentiation medium, DMEM medium containing 10 μg / ml insulin, 10 μg / ml transferrin, 100 μM dexamethasone, 100 μM indomethacin, and 1% fetal bovine serum was used. Ethanol used as a dilution solvent when adding essential oil to differentiation medium as a control (final concentration 0.01% in the medium), and caffeine as a positive control that has been reported to suppress fat accumulation in fat cells (final in medium) Culturing was carried out by adding 200 μg / ml) to the differentiation medium, and compared with the case where essential oil was added.
After differentiation culture for 7 days, the number of viable cells and the amount of neutral fat accumulated in the cells were measured to evaluate the effect of various essential oils on fat accumulation.

<Method for measuring the number of living cells>
After completion of differentiation culture for 7 days, trypsin is added to each well and the cells are detached. Trypan blue was added to stain viable cells, and the number of viable cells was counted with a hemocytometer under a microscope.

<Method for determining the amount of neutral fat>
After measuring the number of viable cells, each well was washed twice with PBS (0.5 ml / well) and accumulated in fat cells using a neutral fat measurement kit (Lipidos Liquid Ono Pharmaceutical Co., Ltd.) Sex fat mass was measured.
That is, cells are lysed and existing free glycerol is erased with glycerol kinase, then lipoprotein kinase is added to decompose neutral fat (triglyceride) to produce glycerol, and the produced glycerol is used as a chromogenic substrate. The amount of triglycerides in the cells was quantified by quantifying with the enzyme reaction method used. A calibration curve was prepared using triolein.

<Result>
In FIG. 1, the number of living cells and the amount of neutral fat per cell when various essential oils are added are shown as relative% when the target value is 100, respectively.
As is clear from Fig. 1, eucalyptus oil, laurel oil, grapefruit oil, rosemary oil, clove bud oil, chamomile oil, rose oil, geranium oil, neroli oil, pine needle oil, hyssop oil, fennel oil, clary sage oil, In addition, in the case of adding galvanum oil, a remarkable effect of suppressing fat accumulation was confirmed. Moreover, it was confirmed that the fat accumulation inhibitory effect is higher than caffeine, which has been known to have a fat accumulation inhibitory effect.

In particular, fennel oil, geranium oil, chamomile oil, rosemary oil, hyssop oil, clary sage oil, laurel oil, galvanum oil, and grapefruit oil have a small change in the number of living cells and have little effect on cells. It is considered to have a desirable effect.
Examples of suitable formulations for carrying out the present invention are given below, but the technical scope of the present invention is not limited by these examples.

<Prescription Example 1 Composition for inhibiting fat accumulation>
(Prescription) (mass%)
1. Lemon oil 10
2. Bergamot oil 30
3. Grapefruit oil 10
4). Galvanum oil 0.2
5. Linalool 6
6). Linalyl acetate 8
7). Fennel oil 0.3
8). Geranium oil 1.5
9. Lavender oil 2
10. Citronellol 1
11. Citral 0.5
12 Geranyl acetate 0.5
13. Methyl ionone 1
14 Methyl dihydrojasmonate 10
15. Patchouli oil 1
16. Hexylcinnamic aldehyde 2
17. Helionar 1
18. Coumarin 1
19. Belt fix 2
20. Clarisage oil 1
21. Sandalwood oil 1
22. Evernil 0.5
23. Rosemary oil 1
24. Eugenol 2
25. Dipropylene glycol 6.5

<Prescription Example 2 Composition for inhibiting fat accumulation>
(Prescription) (mass%)
1. Lemon oil 5
2. Bergamot oil 15
3. Grapefruit oil 3
4). Linalool 3
5. Linalyl acetate 5
6). Geranium oil 3
7). Lavender oil 10
8). Citronellol 1
9. Methyl ionone 1
10. Methyl dihydrojasmonate 2
11. Patchouli oil 2
12 Coumarin 1
13. Betylbenyl acetate 2
14 Clarisage oil 3
15. Hyssop oil 1
16. Laurel oil 0.5
17. Chamomile oil 1
18. Eucalyptus oil 10
19. Sandalwood oil 4
20. Evernil 0.5
21. Rosemary oil 5
22. Eugenol 1
23. Dipropylene glycol 2
24. Garaxolid 50% 4
25. Betilbene oil 3
26. Iso E Super 10
27. Benzyl acetate 2

<Prescription example 3 lotion>
(Prescription) (mass%)
1. Glycerin 2
2. Dipropylene glycol 2
3. PEG-60 hydrogenated castor oil 0.3
4). Xylitol 3
5. Ascorbic acid 0.005
6). EDTA3 sodium 0.1
7). Dye proper amount8. 8. Composition for inhibiting fat accumulation according to the present invention Purified water remaining amount 10. Caffeine 0.1

<Formulation example 4 lotion>
(Prescription) (mass%)
1. Alcohol 30
2. Butylene glycol 4
3. Glycerin 2
4). PPG-13 decyltetrades 24 0.3
5. Octyl methoxycinnamate 0.1
6). Menthol 0.2
7). Tranexamic acid 1.0
8). EDTA3 sodium 0.1
9. Dye proper amount
Ten. Composition for inhibiting fat accumulation of the present invention
11． Purified water remaining

<Formulation Example 5 Latex>
(Prescription) (mass%)
1. Ethyl alcohol 10
2. Glycerin 3
3. Butylene glycol 2
4). Polyethylene glycol 3
5. Carboxyvinyl polymer 0.1
6). Acrylic acid / alkyl acrylate copolymer 0.1
7). Caustic potash 0.1
8). Cyclomethicone 4
9. Squalane 2
Ten. Spherical polyethylene 2
11． Menthol 0.5
12． Corade Cavallo 0.3
13. Paraben appropriate amount
14. EDTA3 sodium 0.1
15． Pigment appropriate amount
16． Composition for inhibiting fat accumulation of the present invention
17． Purified water remaining

<Formulation Example 6 Latex>
(Prescription) (mass%)
1. Butylene glycol 4
2. Propylene glycol 4
3. Carboxyvinyl polymer 0.2
4). Caustic potash 0.2
5. Behenic acid 0.5
6). Stearic acid 0.5
7). Isostearic acid 0.5
8). Glyceryl stearate 1
9. Glyceryl isostearate 1
Ten. Behenyl alcohol 0.5
11． Batyl alcohol 0.5
12． Squalane 5
13. Trioctanoin 3
14. Phenyltrimethicone 2
15． Beech bud extract 0.5
16． Appropriate amount of phenoxyethanol
17． EDTA3 sodium 0.1
18． Pigment appropriate amount
19. Composition for inhibiting fat accumulation of the present invention
20． Purified water remaining
twenty one. Caffeine 0.3

<Prescription Example 7 Latex>
(Prescription) (mass%)
1. Glycerin 3
2. Xylitol 2
3. Carboxyvinyl polymer 0.1
4). Caustic potash 0.1
5. Glyceryl isostearate 1
6). Glyceryl stearate 0.5
7). Behenyl alcohol 1
8). Batyl alcohol 1
9. Palm hardened oil 2
Ten. Vaseline 1
11． Squalane 5
12． Erythrityl octoate 3
13. Cyclomethicone 1
14. Ascorbic acid phosphate magnesium 0.5
15． Paraben appropriate amount
16． EDTA3 sodium 0.1
17． Composition for inhibiting fat accumulation of the present invention
18． Purified water remaining

<Prescription Example 8 Cream>
(Prescription) (mass%)
1. Glycerin 10
2. Butylene glycol 5
3. Carboxyvinyl polymer 0.1
4). Caustic potash 0.2
5. Stearic acid 2
6). Glyceryl stearate 2
7). Glyceryl isostearate 2
8). Vaseline 5
9. Stearyl alcohol 2
Ten. Behenyl alcohol 2
11． Palm hardened oil 2
12． Squalane 10
13. α-Glucosyl hesperidin 0.1
14. Paraben appropriate amount
15． EDTA3 sodium 0.1
16． Pigment appropriate amount
17． Composition for inhibiting fat accumulation of the present invention
18． Purified water remaining
19. Caffeine 0.5

<Prescription Example 9 Cream>
(Prescription) (mass%)
1. Glycerin 3
2. Dipropylene glycol 7
3. Polyethylene glycol 3
4). Glyceryl stearate 3
5. Glyceryl isostearate 2
6). Stearyl alcohol 2
7). Behenyl alcohol 2
8). Liquid paraffin 7
9. Cyclomethicone 3
Ten. Dimethicone 1
11． Octyl methoxycinnamate 0.1
12． Vitamin A acetate 0.5
13. Appropriate amount of phenoxyethanol
14. EDTA3 sodium 0.1
15． Pigment appropriate amount
16． Composition for inhibiting fat accumulation of the present invention
17． Purified water remaining

<Prescription Example 10 Gel>
(Prescription) (mass%)
1. Ethyl alcohol 10
2. Glycerin 5
3. Butylene glycol 5
4). Carboxyvinyl polymer 0.5
5. AMP 0.3
6). PEG-60 hydrogenated castor oil 0.3
7). Menthol 0.02
8). Oil-soluble licorice extract (root) 0.2
9. Paraben appropriate amount
Ten. EDTA3 sodium 0.1
11． Composition for inhibiting fat accumulation of the present invention
12． Purified water remaining

<Prescription Example 11 Aerosol>
(Prescription) (mass%)
1. Glycerin 2
2. Dipropylene glycol 2
3. PEG-60 hydrogenated castor oil 0.3
4). Corade cavalo (root leaves) 0.5
5. Paraben appropriate amount 6. EDTA3 sodium 0.1
7). Dye proper amount8. 8. Composition for inhibiting fat accumulation according to the present invention Purified water remaining
Ten. Nitrogen gas 0.8
11． Caffeine 0.1

<Prescription Example 12 Aerosol>
(Prescription) (mass%)
1. Alcohol 15
2. Butylene glycol 2
3. Glycerin 1
4). PPG-13 Decyltetrades 24 0.1
5.4 Potassium 4-methoxysalicylate 0.5
6). EDTA3 sodium 0.1
7). Dye proper amount8. 8. Composition for inhibiting fat accumulation according to the present invention Purified water remaining
Ten. LPG 40

<Prescription Example 13 Bath Agent>
(Prescription) (mass%)
1. Sodium sulfate 45.0
2. Sodium bicarbonate 45.0
3. Hyssop oil 10.0
4). Composition for inhibiting fat accumulation of the present invention

<Formulation Example 14 Foam Pack>
(Prescription) (mass%)
1. Caffeine 1.0
2. Sodium metaphosphate 0.02
3. Trehalose 2.0
4). Glycerin 7.0
5. Methylparaben 0.1
6). Potassium hydroxide 0.15
7). Stearic acid 0.5
8). Myristic acid 1.0
9. Batyl alcohol 1.5
Ten. Polyoxyethylene (60) hydrogenated castor oil 3.0
11． Fennel oil 0.3
12． Liquefied petroleum gas 6.0
13. Dimethyl ether 3.0
14. Composition for inhibiting fat accumulation of the present invention
15． Purified water residue

<Prescription Example 15 Shampoo>
(Prescription) (mass%)
1. Lauryl polyethylene (3) sulfate sodium salt 10.0
2. Lauryl sulfate sodium salt 5.0
3. Palm oil fatty acid diethanolamide 4.0
4). Glycerin 1.0
5. 5. Composition for inhibiting fat accumulation according to the present invention Appropriate amount of dye 7. Paraben appropriate amount 8. EDTA3 sodium 0.1
9. Citric acid 0.05
Ten. Sodium citrate 0.05
11． Purified water residue

<Prescription Example 16 Shampoo>
(Prescription) (mass%)
1. Lauryl polyethylene (3) sulfate triethanolamine salt
5.0
2. Lauryl polyethylene (3) sulfate sodium salt
5.0
3. Lauryl sulfate sodium salt 5.0
4). Lauroyl monoethanolamide 1.0
5. Lauryldimethylaminoacetic acid betaine 5.0
6). Cationized cellulose 7.0
7). Ethylene glycol distearate 2.0
8). Protein derivative 0.5
9. Composition for inhibiting fat accumulation of the present invention
Ten. EDTA3 sodium 0.1
11． Citric acid 0.05
12． Sodium citrate 0.05
13. Purified water residue
14. Caffeine 0.3

<Prescription Example 17 Rinse>
(Prescription) (mass%)
1. Silicone oil 3.0
2. Liquid paraffin 1.0
3. Cetyl alcohol 1.5
4). Stearyl alcohol 1.0
5. Stearyltrimethylammonium chloride 0.7
6). Glycerin 3.0
7). 7. An appropriate amount of the composition for inhibiting fat accumulation according to the present invention 8. Appropriate amount of dye Appropriate amount of phenoxyethanol
Ten. Purified water residue

<Prescription Example 18 Fiber>
Microcapsules containing cupro ammonium cellulose solution (cellulose concentration 10% by mass, ammonium concentration 7% by mass, copper concentration 3.6% by mass) containing the essential oil of the present invention (particle size 50 μm or less, proportion of essential oil in microcapsules) Is added within a range of 0.1 to 20% by mass with respect to the mass of cellulose, and is then spun in accordance with a normal wet yarn-proofing method to obtain fibers through a scouring step and a drying step. .

<Prescription Example 19 Ice Cream>
(mass%)
1. Nonfat dry milk 8.8
2. 5.5 sugar
3. Maltose Mizuame 15.6
4). Erythritol 4.4
5. Sunflower oil 11.7
6). Palm oil 1.3
7). Emulsification stabilizer (Sunsoft Super 250: manufactured by Taiyo Chemical Co., Ltd.) 0.3
8). Water 52.0
9. Composition for inhibiting fat accumulation of the present invention 0.2
10. Vanilla flavor (made by Showa Agricultural Co., Ltd.) 0.2
(Manufacturing method)
1 to 8 were mixed and dissolved uniformly, heated to 65 ° C., homogenized in two stages (150 kg / cm ² and 50 kg / cm ² ), sterilized at 85 ° C. for 15 seconds, and rapidly cooled to 5 ° C. or lower. After adding 9, 10 and mixing uniformly, aging was performed overnight in the tank. This mix was frozen with a continuous freezer at an overrun of 30%, filled into a paper cup for frozen dessert at a temperature of -7.0 to -8.0 ° C, and quickly frozen in a freezer at -35 ° C.

<Prescription Example 20 Dry Dog Food>
Mix and grind a composition comprising 30 parts of corn, 30 parts of wheat flour, 15 parts of defatted soybean, 10 parts of meat meal, 5 parts of chicken meal, 1 part of vitamin mineral mix, and 1 part of the composition for inhibiting fat accumulation of the present invention. The size was adjusted using a sieve having an opening of 1 mm. Water was added to the obtained composition so as to be about 27% by mass, an 8 mm circular die was attached to the cooking extruder, and extrusion treatment was performed to obtain an expanded product. The obtained expanded product was dried at 120 ° C. for 20 minutes by a belt dryer, and then 5 parts of soybean oil was sprayed on 95 parts of the expanded product to obtain a dry dog food.

It is the figure which showed the number of living cells at the time of adding various essential oils, and the amount of triglycerides per cell by relative%.

Claims (4)

One or more essential oils selected from the group consisting of Laurel Oil, Grapefruit Oil, Clove Bud Oil, Rose Oil, Geranium Oil, Neroli Oil, Pine Needle Oil, Hyssop Oil, Fennel Oil, Clarisage Oil, and Galvanum Oil And a fat accumulation inhibitor characterized by directly acting on fat cells. A skin external preparation for fat accumulation suppression, comprising one or two essential oils selected from the group consisting of neroli oil and pine needle oil. A slimming cosmetic comprising one or two essential oils selected from the group consisting of neroli oil and pine needle oil. An obesity-improving agent comprising one or two essential oils selected from the group consisting of neroli oil and pine needle oil.

Images