JP2005194252A - Fat accumulation-suppressing composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a fat accumulation-suppressing composition which exerts excellent fat accumulation-suppressing effect by directly acting on a fat cell and also has no limit in flavoring. <P>SOLUTION: This fat accumulation-suppressing composition contains a modified grape fruit oil in which limonene of 10-100 mass% is removed from the fruit oil. Alternatively, the composition contains an extract from the grape fruit oil by a mixed solvent comprising n-hexane/ethyl acetate of 9:1. In this invention, there are disclosed the composition-containing skin care preparation for external use, slimming cosmetic, obesity improver, clothes and foods. Further, there is provided a fat accumulation-suppressing method comprising application of the composition to the skin. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a composition for suppressing fat accumulation, particularly a composition containing grapefruit oil.

Obesity is a hotbed for various lifestyle-related diseases, and improving obesity is important for maintaining health. There are many people who are obese in modern society with excessive caloric intake and lack of exercise. In Japan, there are about 13 million men and 10 million women who exceed BMI (physique index) 25, which is the standard for obesity. It is said that providing a method for improving obesity is an important issue. The cause of obesity is that excessive calories ingested are stored in adipose tissue (adipocytes) as neutral fat.
For the purpose of developing a drug having a slimming effect, screening of various herbal medicines, extracts, drugs and the like showing effects such as suppression of fat synthesis and promotion of lipolysis is performed. For example, it has already been reported that caffeine and capsicum ethanol extract reduce the amount of triglycerides (JP 08-169896, Grant-in-Aid for Scientific Research No. 12, p.163-168 (1999)). )). However, their effects are not sufficient and little direct action on adipocytes has been studied.

On the other hand, essential oils obtained by steam-distilling plants have conventionally been known for various pharmacological actions such as bactericidal action, anti-inflammatory action, and sedative action. Searching for essential oils that have effects such as suppression of fat synthesis and promotion of lipolysis is an important issue in improving obesity and maintaining people's health, and these essential oils are external preparations for skin, clothing, food, etc. It is considered that the industrial utility value is large.
Then, as a result of studying various essential oils, the present inventors have found that smelling the aroma component of grapefruit oil has an anti-obesity effect (JP 2002-193824, JP 2002-265977, etc.).
JP 08-169896 JP2002-193824 JP 2002-265977 No.12, p.163-168 (1999)

However, since grapefruit oil has a strong citrus fragrance, when used in cosmetics and the like, there is a drawback that even if other fragrances are blended, the citrus fragrance cannot be used as a whole. Moreover, the effect originates in the effect | action which activates a sympathetic nervous system by a fragrance, and the direct effect | action to an adipocyte has not been reported.
The present invention has been made in view of the problems as described above, and its purpose is to directly act on fat cells to exert an excellent fat accumulation-inhibiting effect, and the composition for inhibiting fat accumulation in which the aroma is not limited. Is to provide.

As a result of studying to solve the above-mentioned problems, the present inventors have found that removing the main component limonene from grapefruit oil weakens the citrus fragrance and exerts an effect of suppressing fat accumulation, thereby completing the present invention. .
That is, the first subject of the present invention is a composition for inhibiting fat accumulation, comprising a modified grapefruit oil obtained by removing 10-100% by mass of limonene from grapefruit oil. Or it is the composition for fat accumulation suppression characterized by including the extract by the mixed solvent of n-hexane / ethyl acetate = 9: 1 of grapefruit oil.
The second subject of the present invention is an external preparation for skin, a slimming cosmetic, an obesity-improving agent, clothing, and a food comprising the composition.
The third subject of the present invention is a method for inhibiting fat accumulation, characterized in that the composition is applied to the skin.

  According to the present invention, by removing limonene from grapefruit oil, the citrus aroma is weakened and an excellent fat accumulation suppressing effect is exhibited. Therefore, a fat accumulation-inhibiting composition that has a high fat accumulation-inhibiting effect and is not limited in fragrance, an external preparation for skin containing the composition, a slimming cosmetic, an obesity-improving agent, clothing, food, or the composition on the skin A method for suppressing fat accumulation to be applied can be provided.

Hereinafter, preferred embodiments of the present invention will be described.
The present invention was carried out by focusing on the fact that grapefruit oil has an effect of directly acting on adipose tissue and suppressing the accumulation of fat in fat cells, and limonene as a main component from grapefruit oil. It has been found that even if it is removed, the effect of suppressing fat accumulation is exhibited.
<Grapefruit oil>
Grapefruit is the scientific name Citrus paradis Macf. And is an evergreen tree of the citrus family native to the West Indies. The main component is limonene and has a unique citrus scent.
Grapefruit oil is obtained by pressing the skin simultaneously with the production of fruit juice.

本発明において、改質グレープフルーツオイルは、グレープフルーツオイルからリモネンを１０〜１００質量％除去したものであるが、特にリモネンを実質的に全量除去することが好ましい。 Limonene is a colorless liquid with a lemon-like fragrance and is an optical isomer of dextrorotatory d (+)-and levorotatory l (-)-(specific gravity 0.84, boiling point 175 ° C, flash point 48 ° C, molecular weight 136). ,twenty four).

In the present invention, the modified grapefruit oil is one obtained by removing limonene from 10 to 100% by mass from the grapefruit oil, and it is particularly preferable to remove substantially all of the limonene.

The composition for suppressing fat accumulation according to the present invention can also contain an essential oil other than the modified grapefruit oil as long as the effects of the present invention are not impaired. The modified grapefruit oil removes limonene and has a weak citrus fragrance, so that it can be made into a desired scent by adding essential oil, synthetic fragrance or the like.
The composition for suppressing fat accumulation according to the present invention can be applied to an external preparation for skin, food, feed, clothing, footwear, accessories and the like.

  The external preparation for skin of the present invention, in addition to the above-described composition for suppressing fat accumulation, is not limited to the effects of the present invention, and other components usually used in cosmetics, quasi drugs, pharmaceuticals, etc., for example, powder Ingredients, liquid fats and oils, solid fats and oils, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, silicones, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, humectants, high water solubility Molecule, thickener, film agent, UV absorber, sequestering agent, lower alcohol, polyhydric alcohol, sugar, amino acid, organic amine, polymer emulsion, pH adjuster, skin nutrient, vitamin, antioxidant, Antioxidant assistants, fragrances, water and the like can be appropriately blended as necessary, and can be produced by conventional methods according to the intended dosage form.

  Other components that can be blended include, for example, preservatives (ethyl paraben, butyl paraben, etc.); anti-inflammatory agents (eg, glycyrrhizic acid derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, zinc oxide, allantoin, etc.); Extract, placenta extract, saxifrage extract, arbutin, etc.); various extracts (eg, buckwheat, auren, shikon, peonies, assembly, birch, sage, loquat, carrot, aloe, mallow), iris, grape, yokuinin, loofah, lily , Saffron, nematode, ginger, hypericum, onionis, garlic, red pepper, chimney, red snapper, seaweed, etc.), activator (eg, royal jelly, photosensitizer, cholesterol derivative, etc.); Acid Gyl ester, nicotinic acid β-butoxyethyl ester, capsaicin, gingerone, cantalis tincture, ictamol, tannic acid, α-borneol, tocopherol nicotinate, inositol hexanicotinate, cyclandrate, cinnarizine, trazoline, acetylcholine, verapamil, cephalanthin , Γ-oryzanol, etc.); antiseborrheic agents (eg, sulfur, thianthol, etc.); anti-inflammatory agents (eg, tranexamic acid, thiotaurine, hypotaurine, etc.) and the like.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, malic acid and other metal sequestering agents, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice, Various herbal extracts such as Karin, Ichiyakusou, drugs such as tocopherol acetate, glycyrrhizic acid, glycyrrhizic acid and its derivatives or salts thereof, whitening agents such as vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, Amino acids such as arginine and lysine and derivatives thereof, saccharides such as fructose, mannose, erythritol, trehalose and xylitol can also be appropriately blended.

  The external preparation for skin of the present invention can be widely applied to cosmetics, pharmaceuticals, and quasi drugs applied to the outer skin. The dosage form is arbitrary, such as solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, gel, aerosol, mist, capsule, etc. It can be provided in the form. In addition, the product form of the external preparation for skin of the present invention is also arbitrary, body cosmetics such as massage agents and foot sprays; bath preparations; facial cosmetics such as lotions, emulsions, creams, packs, etc .; foundations, funny, blushers, Makeup cosmetics such as lipstick, eye shadow, eyeliner, mascara and sunscreen; aromatic cosmetics; skin cleansing agents such as make-up removers, facial cleansers, body shampoos; hair cosmetics such as hair rinses and shampoos; ointments; It can be applied in any form as long as it is used for conventional skin external preparations such as paper.

The blending amount of the modified grapefruit oil from which limonene has been removed in the external preparation for skin is not particularly limited, but is preferably 0.001 to 50% by mass, more preferably 0.002 to 30% by mass of the entire composition. If it is less than 0.001% by mass, a sufficient fat accumulation inhibitory effect may not be observed, and even if it exceeds 50% by mass, a further increase in fat accumulation inhibitory effect is hardly observed.
In addition, as food, it can be applied to bread, rice cakes, noodles, dairy products, edible oils and fats, confectionery, jam, honey, egg products, beverages and the like. Furthermore, the feed can be applied to livestock feed, pet food and the like.
As clothing, it can be applied to underwear, socks, stockings, girdle, nightwear and the like.

Hereinafter, preferred embodiments of the present invention will be described in more detail. In addition, this invention is not limited by this. Further, the blending amount (%) is mass% unless otherwise specified.
<GC / MS analysis of grapefruit oil>
First, in order to analyze the components of grapefruit oil, which is said to have an effect of suppressing fat accumulation, grapefruit oil (Charabo) is dissolved in acetone and analyzed by gas chromatography / mass spectrometry (GC / MS). Was done.
GC / MS analysis was performed using the 6890N system (Aligent) under the following analysis conditions. Column: INNOWax (60 m × 0.25 mm × 0.25 μm, Aligent), temperature increase: 6 ° C./min (60 ° C. → 96 ° C.), 96 ° C., 1 minute hold, 4 ° C./min (96 ° C. → 230 ° C. ), Maintained at 230 ° C. for 50 minutes, carrier gas: 1 ml / min of helium, ionization voltage: 70 eV.
The detected peaks were identified using the Wiley 275 mass spectral library.

(Table 1)
Grapefruit oil (mass%)
dl-Limonene 93.0
β-Myrcene 1.47
cis-Limonene oxide 0.64
α-Pinene 0.61
Sabinen 0.45
β-terpinene 0.17
Other 3.66
Total 100.0
As shown in Table 1, grapefruit oil is mainly composed of limonene (93.0% by mass), which causes a strong citrus fragrance.

<Limonene's effect of suppressing fat accumulation>
Next, it was investigated whether limonene, the main component of grapefruit oil, had an effect of suppressing fat accumulation.
(1) Culture of adipocytes Rat subcutaneous fat-derived preadipocytes (Toyobo) are seeded in a 24-well plate to a density of 2-3 × 10 ⁴ cells / well, and 10% fetal bovine serum is added until confluent. Growth culture was performed in a DMEM medium containing 37 ° C. and 5% CO ₂ .
After reaching confluence, differentiation medium (10 μg / ml insulin, 10 μg / ml transferrin, 100 μM dexamethasone, 100 μM) with the addition of grapefruit oil or limonene to a final concentration of 0.005%, 0.01% and 0.02% in the medium, respectively. The culture medium was replaced with indomethacin and a DMEM medium containing 1% fetal bovine serum, and differentiation culture was performed.
As a control, ethanol (final concentration 0.01% in the medium) used as a dilution solvent for the essential oil was added to the differentiation medium and cultured.
After 7 days of differentiation culture, the fat accumulation inhibitory effect was evaluated by measuring the amount of neutral fat accumulated in the cells.

(2) Quantification of triglyceride content After measuring the number of living cells, each well was washed twice with PBS (0.5 ml / well), and a triglyceride measurement kit (Lipidos Liquid: Ono Pharmaceutical Co., Ltd.) was used. The amount of triglyceride accumulated in the fat cells was measured.
That is, cells are lysed and existing free glycerol is erased with glycerol kinase, then lipoprotein kinase is added to decompose neutral fat (triglyceride) to produce glycerol, and the produced glycerol is used as a chromogenic substrate. The amount of triglycerides in the cells was quantified by quantifying with the enzyme reaction method used. A calibration curve was prepared using triolein. The ratio of the amount of triglyceride to the control (culture with 0.01% ethanol added) was calculated and used as the triglyceride content (relative%).

FIG. 1 shows the amount of neutral fat per cell in the case of adding grapefruit oil or limonene, as a percentage relative to the control (cultured with 0.01% ethanol) as 100.
As is clear from FIG. 1, grapefruit oil has a fat accumulation suppressing effect, whereas limonene, which is the main component of grapefruit oil, has no fat accumulation suppressing effect, but rather the amount of neutral fat increased. From this result, it was confirmed that the fat accumulation suppressing effect of grapefruit oil was not derived from limonene as the main component.
Therefore, the present inventors tried to remove limonene that causes citrus scent from grapefruit oil.

<Fractionation of grapefruit oil>
Wakogel C-200 was wet-packed with n-hexane, and grapefruit oil (Charabo) was loaded onto a 20 g column. Elution was performed with n-hexane, n-hexane / ethyl acetate = 9: 1, and ethyl acetate in this order, and fractionated into three fractions.
(Table 2)
Elution Solvent Elution Yield (%)
n-Hexane fraction 1 80.3
n-hexane / ethyl acetate = 9/1 Fraction 2 4.5
Ethyl acetate fraction 3 2.7

<GC / MS analysis of each fraction>
Fractions 1 to 3 were each dissolved in acetone, and component analysis was performed by GC / MS. Analysis conditions are as described above. The obtained fraction was immediately concentrated and dried with a rotary evaporator and used for the test.
The results are shown in Tables 3-5.

(Table 3)
Fraction 1 (mass%)
dl-Limonene 96.1
β-Myrcene 1.7
Sabinen 0.8
Caryophyllene 0.4
Terpinene 0.3
β-pinene 0.3
Kajinen 0.1
Other 0.3
Total 100.0

(Table 4)
Fraction 2 (mass%)
Decanal 27.1
Octanal 10.0
Linalool 7.8
Citral 7.4
Noot Katon 6.2
Geranyl acetate 5.5
Citronella 3.6
Nonanal 3.5
Dodecanal 3.3
Citral 3.3
Neryl acetate 2.3
Nerolidol 1.1
Kamfen 0.7
Other 17.9
Total 100.0

(Table 5)
Fraction 3 (mass%)
Octanal 25.1
Terpineol 16.3
Ostole 12.4
Hexadecanoic acid 5.2
Decanol 3.4
Noot Katong 2.9
Nonanol 2.1
Citronellol 1.5
Nellore 1.5
Geraniol 1.3
Carbelol 1.0
Others 27.3
Total 100.0

  As is apparent from Tables 3 to 5, limonene is contained only in fraction 1 and is hardly contained in fractions 2 and 3. Therefore, it can be seen that substantially all of the limonene can be removed from the grapefruit oil by the above fractionation method. Fractions 2 and 3 had a weak citrus fragrance.

<Fat accumulation suppression effect of modified grapefruit oil>
Next, the fat accumulation inhibitory effect of the modified grapefruit oil obtained by removing limonene from the grapefruit oil was tested.
(1) Culture of adipocytes Rat subcutaneous fat-derived preadipocytes (Toyobo) are seeded in a 24-well plate to a density of 2-3 × 10 ⁴ cells / well, and 10% fetal bovine serum is added until confluent. Growth culture was performed in a DMEM medium containing 37 ° C. and 5% CO ₂ .
After reaching confluence, differentiation medium (10 μg / ml insulin, 10 μg / ml transferrin, 100 μM dexamethasone, 100 μM indomethacin, grapefruit oil, fractions 1 to 3 added to a final concentration of 0.02% in the medium) And DMEM medium containing 1% fetal bovine serum), and differentiation culture was performed.

The differentiation medium was ethanol (final concentration 0.01% in the medium) used as a diluent for essential oils as a control, and caffeine (final concentration 200 μg / ml in the medium), which has been reported to inhibit fat accumulation in fat cells, as a positive control. And added to the culture.
After differentiation culture for 7 days, the amount of triglyceride accumulated in the cells and the number of viable cells were measured to evaluate the effect of various essential oils on fat accumulation.

(2) Quantification of the amount of triglyceride In the same manner as described above, the ratio of the triglyceride to the control (culture with 0.01% ethanol added) was calculated to obtain triglyceride (relative%).
(3) Measurement of cell number After completion of differentiation culture for 7 days, 10% alamar blue was added to each well, the absorbance at 544 nm was measured, and the absorbance with respect to the control (culture with 0.01% ethanol). The ratio was calculated as the number of living cells (relative%).
(4) Results Fig. 2 shows the number of viable cells and the amount of neutral fat per cell when caffeine, grapefruit oil, or fractions 1 to 3 are added. It was expressed as a relative% with respect to 100.

As can be seen from FIG. 2, when the fraction 2 which is a modified grapefruit oil is added, the amount of neutral fat per cell is greatly reduced, and the effect is compared with the case where the positive control caffeine is added. The neutral fat reduction effect was almost the same as when grapefruit oil was added.
In addition, when caffeine or grapefruit oil is added, the number of viable cells decreases to about 40%, whereas when fraction 2 which is a modified grapefruit oil is added, viable cells are reduced. It can be seen that it does not decrease and does not adversely affect the cells.

On the other hand, in the fraction 1 containing limonene as a main component (96.1%), the effect of reducing neutral fat was similar to that of caffeine and was not sufficient. Moreover, the number of living cells has decreased to about 40%.
As described above, the modified grapefruit oil from which limonene has been removed is very suitable for a composition for suppressing fat accumulation because it has a weak citrus aroma and exhibits a high neutral fat reduction effect without adversely affecting cells. It was confirmed that there was.
This effect is exhibited when limonene is removed from grapefruit oil in an amount of 10 to 100% by mass, particularly substantially all.

Furthermore, as shown in FIG. 3, it was found that the fat accumulation inhibitory effect of the modified grapefruit oil (here, fraction 2) increases in a concentration-dependent manner.
Although the mechanism of the fat accumulation inhibitory effect of the modified grapefruit oil has not been clarified, it is considered that components other than limonene in the grapefruit oil are combined and act directly on the adipose tissue (adipocytes).

Examples of suitable formulations for carrying out the present invention are listed below, but the technical scope of the present invention is not limited by these examples. Here, the above fraction 2 was used as the modified grapefruit oil.
<Formulation Example 1 Fragrance>
(mass%)
1. Alcohol 75
2. Purified water Residual 3. Dipropylene glycol 5
4). Blended fragrance 10
5). Antioxidant appropriate amount 6. Modified grapefruit oil 5
7). Appropriate amount of dye

<Formulation example 2 lotion>
(mass%)
1. Glycerin 2
2. Dipropylene glycol 2
3. PEG-60 hydrogenated castor oil 0.3
4). Xylitol 3
5). Ascorbic acid 0.005
6). EDTA3 sodium 0.1
7). Dye proper amount8. Modified grapefruit oil Appropriate amount 9. Purified water remaining

<Formulation example 3 lotion>
(mass%)
1. Alcohol 30
2. Butylene glycol 4
3. Glycerin 2
4). PPG-13 decyltetrades 24 0.3
5). Octyl methoxycinnamate 0.1
6). Menthol 0.2
7). Tranexamic acid 1.0
8). EDTA3 sodium 0.1
9. Appropriate amount of dye
Ten. Modified grapefruit oil
11． Purified water remaining

<Formulation Example 4 Latex>
(mass%)
1. Ethyl alcohol 10
2. Glycerin 3
3. Butylene glycol 2
4). Polyethylene glycol 3
5). Carboxyvinyl polymer 0.1
6). Acrylic acid / alkyl acrylate copolymer 0.1
7). Caustic potash 0.1
8). Cyclomethicone 4
9. Squalane 2
Ten. Spherical polyethylene 2
11． Menthol 0.5
12． Xylitol 3
13. Preservative appropriate amount
14. EDTA3 sodium 0.1
15． Pigment appropriate amount
16． Modified grapefruit oil
17． Purified water remaining

<Formulation Example 5 Latex>
(mass%)
1. Butylene glycol 4
2. Propylene glycol 4
3. Carboxyvinyl polymer 0.2
4). Caustic potash 0.2
5). Behenic acid 0.5
6). Stearic acid 0.5
7). Isostearic acid 0.5
8). Glyceryl stearate 1
9. Glyceryl isostearate 1
Ten. Behenyl alcohol 0.5
11． Batyl alcohol 0.5
12． Squalane 5
13. Trioctanoin 3
14. Phenyltrimethicone 2
15． Beech bud extract 0.5
16． Appropriate amount of phenoxyethanol
17． EDTA3 sodium 0.1
18． Pigment appropriate amount
19. Modified grapefruit oil
20． Purified water remaining

<Formulation Example 6 Latex>
(mass%)
1. Glycerin 3
2. Xylitol 2
3. Carboxyvinyl polymer 0.1
4). Caustic potash 0.1
5). Glyceryl isostearate 1
6). Glyceryl stearate 0.5
7). Behenyl alcohol 1
8). Batyl alcohol 1
9. Palm hardened oil 2
Ten. Vaseline 1
11． Squalane 5
12． Erythrityl octoate 3
13. Cyclomethicone 1
14. Ascorbyl phosphate magnesium 0.5
15． Paraben appropriate amount
16． EDTA3 sodium
17． Modified grapefruit oil
18． Purified water remaining

<Formulation example 7 cream>
(mass%)
1. Glycerin 10
2. Butylene glycol 5
3. Carboxyvinyl polymer 0.1
4). Caustic potash 0.2
5). Stearic acid 2
6). Glyceryl stearate 2
7). Glyceryl isostearate 2
8). Vaseline 5
9. Stearyl alcohol 2
Ten. Behenyl alcohol 2
11． Palm hardened oil 2
12． Squalane 10
13. α-Glucosylhasperidin 0.1
14. Paraben appropriate amount
15． EDTA3 sodium 0.1
16． Pigment appropriate amount
17． Modified grapefruit oil
18． Purified water remaining

<Formulation example 8 cream>
(mass%)
1. Glycerin 3
2. Dipropylene glycol 7
3. Polyethylene glycol 3
4). Glyceryl stearate 3
5). Glyceryl isostearate 2
6). Stearyl alcohol 2
7). Behenyl alcohol 2
8). Liquid paraffin 7
9. Cyclomethicone 3
Ten. Dimethicone 1
11． Octyl methoxycinnamate 0.1
12． Vitamin A acetate 0.5
13. Appropriate amount of phenoxyethanol
14. EDTA3 sodium 0.1
15． Pigment appropriate amount
16． Modified grapefruit oil
17． Purified water remaining

<Formulation example 9 gel>
(mass%)
1. Ethyl alcohol 10
2. Glycerin 5
3. Butylene glycol 5
4). Carboxyvinyl polymer 0.5
5). AMP 0.3
6). PEG-60 hydrogenated castor oil 0.3
7). Menthol 0.02
8). Oil-soluble licorice extract (root) 0.2
9. Paraben appropriate amount
Ten. EDTA3 sodium 0.1
11． Modified grapefruit oil
12． Purified water remaining

<Formulation Example 10 Aerosol>
(mass%)
1. Glycerin 2
2. Dipropylene glycol 2
3. PEG-60 hydrogenated castor oil 0.3
4). Corade cavalo (root leaves) 0.5
5). Paraben appropriate amount 6. EDTA3 sodium 0.1
7). Dye proper amount8. Modified grapefruit oil Appropriate amount 9. Purified water remaining
Ten. Nitrogen gas 0.8

<Formulation Example 11 Aerosol>
(mass%)
1. Alcohol 15
2. Butylene glycol 2
3. Glycerin 1
4). PPG-13 Decyltetrades 24 0.1
5.4 Potassium 4-methoxysalicylate 0.5
6). EDTA3 sodium 0.1
7). Dye proper amount8. Modified grapefruit oil Appropriate amount 9. Purified water remaining
Ten. LPG 40

<Formulation example 12 bath agent>
(mass%)
1. Sodium sulfate 45.0
2. Sodium bicarbonate 45.0
3. Hyssop oil 10.0
4). 4. Modified grapefruit oil appropriate amount Purified water residue

<Formulation example 13 foam pack>
(mass%)
1. Caffeine 1.0
2. Sodium metaphosphate 0.02
3. Trehalose 2.0
4). Glycerin 7.0
5). Methylparaben 0.1
6). Potassium hydroxide 0.15
7). Stearic acid 0.5
8). Myristic acid 1.0
9. Batyl alcohol 1.5
Ten. Polyoxyethylene (60) hydrogenated castor oil 3.0
11． Modified grapefruit oil 0.3
12． Liquefied petroleum gas 6.0
13. Dimethyl ether 3.0
14. Purified water residue

<Formulation example 14 shampoo>
(mass%)
1. Lauryl polyethylene (3) sulfate sodium salt 10.0
2. Lauryl sulfate sodium salt 5.0
3. Palm oil fatty acid diethanolamide 4.0
4). Glycerin 1.0
5). Modified grapefruit oil Appropriate amount 6. Appropriate amount of dye 7. Paraben appropriate amount 8. EDTA3 sodium 0.1
9. Citric acid 0.05
Ten. Sodium citrate 0.05
11． Purified water residue

<Formulation example 15 shampoo>
(mass%)
1. Lauryl polyethylene (3) sulfate triethanolamine salt
5.0
2. Lauryl polyethylene (3) sulfate sodium salt
5.0
3. Lauryl sulfate sodium salt 5.0
4). Lauroyl monoethanolamide 1.0
5). Lauryldimethylaminoacetic acid betaine 5.0
6). Cationized cellulose 7.0
7). Ethylene glycol distearate 2.0
8). Protein derivative 0.5
9. Modified grapefruit oil
Ten. Paraben appropriate amount
11． EDTA3 sodium 0.1
12． Citric acid 0.05
13. Sodium citrate 0.05
14. Purified water residue

<Formulation Example 16 Rinse>
(mass%)
1. Silicone oil 3.0
2. Liquid paraffin 1.0
3. Cetyl alcohol 1.5
4). Stearyl alcohol 1.0
5). Stearyltrimethylammonium chloride 0.7
6). Glycerin 3.0
7). Suitable amount of modified grapefruit oil 8. 8. Appropriate amount of dye Appropriate amount of phenoxyethanol
Ten. Purified water residue

<Formulation Example 17 Microencapsulated Fiber>
A microcapsule (grain size of 50 μm or less) encapsulating modified grapefruit oil in a cupro ammonium cellulose solution (cellulose concentration 10 mass%, ammonium concentration 7 mass%, copper concentration 3.6 mass%) is 0 with respect to the cellulose mass. After adding and mixing within the range of 1 to 20% by mass, spinning was performed according to a normal wet yarn-proofing method, and microencapsulated fibers were obtained through a scouring step and a drying step.

<Formulation Example 18 Ice Cream>
(mass%)
1. Nonfat dry milk 8.8
2. 5.5 sugar
3. Maltose Mizuame 15.6
4). Erythritol 4.4
5). Sunflower oil 11.7
6). Palm oil 1.3
7). Emulsification stabilizer (Sunsoft Super 250: manufactured by Taiyo Chemical Co., Ltd.) 0.3
8). Water 52.0
9. Modified grapefruit oil 0.2
Ten. Vanilla flavor (made by Showa Agricultural Co., Ltd.) 0.2
(Manufacturing method)
1 to 8 were mixed and dissolved uniformly, heated to 65 ° C., homogenized in two stages (150 kg / cm ² and 50 kg / cm ² ), sterilized at 85 ° C. for 15 seconds, and rapidly cooled to 5 ° C. or lower. After adding 9, 10 and mixing uniformly, aging was performed overnight in the tank. This mix was frozen with a continuous freezer at an overrun of 30%, filled into a paper cup for frozen dessert at a temperature of -7.0 to -8.0 ° C, and quickly frozen in a freezer at -35 ° C.

<Formulation Example 19 Dry Dog Food>
A composition comprising 30 parts of corn, 30 parts of wheat flour, 15 parts of defatted soybeans, 10 parts of meat meal, 5 parts of chicken meal, 1 part of vitamin mineral mix, and 1 part of the modified grapefruit oil of the present invention is mixed and pulverized. The size was adjusted using a sieve having the following opening. Water was added to the obtained composition so as to be about 27% by mass, an 8 mm circular die was attached to the cooking extruder, and extrusion treatment was performed to obtain an expanded product. The obtained expanded product was dried at 120 ° C. for 20 minutes by a belt dryer, and then 5 parts of soybean oil was sprayed on 95 parts of the expanded product to obtain a dry dog food.

It is the figure which showed the fat accumulation inhibitory effect of grapefruit oil and limonene by relative% of the amount of neutral fat per cell. It is the figure which showed the fat accumulation inhibitory effect by the relative% of the number of living cells and the amount of triglycerides per cell. It is the figure which showed the density | concentration dependence of the fat accumulation inhibitory effect.

Claims (8)

A composition for suppressing fat accumulation, comprising a modified grapefruit oil obtained by removing 10 to 100% by mass of limonene from grapefruit oil. A composition for suppressing fat accumulation, comprising an extract of a grapefruit oil mixed solvent of n-hexane: ethyl acetate = 9: 1. An external preparation for skin comprising the composition according to claim 1 or 2. A slimming cosmetic comprising the composition according to claim 1. An obesity-improving agent comprising the composition according to claim 1 or 2. A garment comprising the composition according to claim 1. A food comprising the composition according to claim 1 or 2. A method for inhibiting fat accumulation, comprising applying the composition according to claim 1 or 2 to the skin.

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