JP4512948B2 - Useful amino acid composition, food or food compounding agent containing the same, and method for producing the same - Google Patents

Description

  TECHNICAL FIELD The present invention relates to a useful amino acid composition derived from fish and shellfish, a food or a food formulation containing the same, and a method for producing the same.

  Seafood is a useful food as a source of protein and other nutrients. In recent years, seafood landed at the port is not just a commercial product, but also a variety of products processed and commercialized at processing plants. Has increased. Various by-products are produced in the process of producing the main product, and if there is no effective usage, it becomes a waste and not only lowers the processing profit, but also in recent years, in particular, disposal costs and disposal sites Issues such as environmental destruction due to securing and disposal are becoming serious. There are many examples of these. For example, squid that is the internal organs of the squid, the epidermis that is peeled off when processed into squid or salted fish, etc. There are scallops that correspond to the internal organs that are by-products.

  By the way, the amount of scallops in Japan reaches 100,000 tons per year, and it is also known that squid gourds are produced in Japan very much and are rich in various substances useful for human health. However, since it is difficult to separate, concentrate and purify these useful substances, most of them are currently discarded, resulting in waste of resources and harmful effects of disposal. Some attempts have been made to solve this problem and to make effective use of materials that are currently discarded.

  Furthermore, seafood waste contains various toxic heavy metals, and cadmium (Cd) contained in scallops and the like is 100 to 200 ppm in terms of wetness of waste. There are concerns about contamination. For this reason, it is necessary to separate and extract useful substances from seafood waste, and at the same time, concentrate harmful substances such as Cd in the residue and perform final treatment.

  As a conventional technique, Patent Document 1 discloses a method for efficiently separating and recovering oil and protein from scallops and squid, and a recovered oil. However, the separation method is not yet precise enough, and the product obtained for this purpose is not a single structural component that can be expressed by a chemical structural formula, for example, and its use is also for food and general human nutrition. Stays.

  Patent Documents 2 to 4 disclose methods for producing useful peptides or amino acids from fish and shellfish proteins by enzymatic degradation.

  Furthermore, prior arts have been disclosed in which useful amino acids from fish and shellfish are obtained by extraction treatment without enzymatic degradation. Patent Document 5 discloses that amino acids are extracted from bonito by hot water extraction.

JP 2003-81996 A JP-A-6-007188 JP-A-8-214841 JP-A-8-231414 JP-A-10-276737

Conventionally, with the increase in waste due to advanced processing of seafood products, disposal costs have increased, the disposal site has been secured, environmental pollution caused by Cd (cadmium) contained in the waste has become serious, and disposal Reducing the amount of waste through the effective use of materials is an important issue for the fishery industry.
On the other hand, it is known that healthy and nutritionally useful components may be abundantly contained in waste, and by efficiently recovering this, it is possible to achieve a reduction in waste and production of useful components It is an important and promising issue.
It has been widely known that fish and shellfish contain a high-quality protein, and thus a high-quality amino acid composition can be obtained by decomposing it. In many cases, this proteolysis is performed by an enzyme.

  Further, fish and shellfish generally contain heavy metals such as high concentrations of iron and Cd. If these cannot be separated and removed efficiently, there is a problem that the active ingredient cannot be separated and recovered.

The present invention is intended to light of the above problems, to provide a method for manufacturing a fish, particularly useful amino acid composition derived from waste from the processing process.

In order to achieve the above object, a method for producing a useful amino acid composition according to the present invention comprises a first step and a second step for removing water and oil from a portion of seafood and taking out a solid, and an organic solvent in the solid. And a third step of removing the organic solvent extract by separating and removing the residual solid, a fourth step of adding an acid to the residual solid and hydrolyzing, a neutralization treatment after the decomposition, and filtering, The method includes a fifth step of obtaining a filtrate and a sixth step of extracting a useful amino acid composition from an effluent obtained by removing heavy metals from the filtrate.
In the above configuration, preferably, the first and second steps include a first steaming step of adding water to a fish and shellfish portion and cooking at a predetermined temperature for a predetermined time, and a second solid liquid by centrifugation and / or filtration. A separation step, and the steaming step and the solid-liquid separation step are continuously performed. In the third step, the organic solvent is ethanol or hydrous acetone. In the fourth step, hydrolysis is preferably performed by adding hydrochloric acid, and the filtrate is passed through the ion exchange resin to remove heavy metals. In the fourth step, preferably, a proteolytic enzyme is further added after hydrolysis, followed by enzymatic degradation to obtain a useful amino acid composition containing a useful peptide.
According to the said structure, the useful amino acid composition which removes a heavy metal from the site | part of seafood, and contains abundantly the amino acid and functional amino acid used as an umami | taste and a sweet taste component can be extracted efficiently. In addition, when a proteolytic enzyme is added for enzymatic degradation, a useful amino acid composition containing a useful peptide can be efficiently extracted.

Preferably, in the sixth step, the filtrate is heated after being adjusted to alkalinity, and further the pH of the filtrate is acidified, the filtrate is filtered to obtain a filtrate and a residue, and heavy metals are removed from the filtrate. An effluent is obtained, and the effluent is decolorized and purified to remove salt and extract a useful amino acid composition.
Preferably, after the salt content is removed in the sixth step, a powdery useful amino acid composition is obtained by a drying step.
In the above structure, the seafood part is a by-product produced from the process of marine processing, and preferably scallops.
According to the said structure, heavy metals, such as Cd contained in the seafood site | part, can be ionized by addition of hydrochloric acid, it can concentrate with an ion exchange resin etc., and it can collect | recover almost completely. For this reason, a useful amino acid composition can be made not to contain a heavy metal.

  In the above-described configuration, the seafood part is preferably a by-product produced from the process of marine processing, particularly scallop. According to the said structure, a useful amino acid composition can be extracted from the scallop uro which is normally discarded as a fishery part.

The present invention removes moisture and oil from the seafood parts and takes out the solid matter, adds an organic solvent to the solid matter, separates and removes the organic solvent extract, takes out the residual solid matter, and removes the acid residue into the residual solid matter. Is added to hydrolyze , and after neutralization, the heavy metal is removed and the useful amino acid composition is extracted. Obtained useful amino acid composition is composed of a amino acids consisting of more than 20 wt% aspartic acid and glutamic acid, 30 wt% or more of serine, glycine, and amino acids consisting of threonine and alanine, 20% or more by weight of arginine and lysine including the amino acid.
Preferably, after hydrolysis, enzymatic degradation by adding protease.
According to the said structure, the useful amino acid composition which contains abundantly functional amino acids, such as an amino acid used as an umami | flavor and sweet taste component which consists of aspartic acid, glutamic acid, serine, glycine, threonine, and alanine, and arginine, and a lysine is obtained.
In addition, when proteolytic enzyme is added to perform enzymatic degradation, a useful amino acid composition containing a useful peptide can be obtained.

As described above, the useful amino acid composition comprises an amino acid composed of 20% by weight or more of aspartic acid and glutamic acid, an amino acid composed of 30% by weight or more of serine, glycine, threonine and alanine, and 20% by weight or more of arginine and lysine. made and an amino acid. Preferably, it further contains a useful peptide.
Thus, aspartic acid, glutamic, serine, glycine, and an amino acid as a flavor and sweetening component consisting threonine or alanine, a useful amino acid composition rich in the functional amino acids such as arginine and lysine in the food or food formulations By adding, useful amino acids can be easily taken from food . For this reason, for example, the useful amino acid composition obtained by the present invention can be effectively used as a seasoning, a cooking dashi, a raw material for processed foods, etc., for tasting a cooked product.

ADVANTAGE OF THE INVENTION According to this invention, the useful amino acid composition derived from fish and shellfishes, or the foodstuff or foodstuff compound containing it can be obtained.
Furthermore, according to the method for producing a useful amino acid composition of the present invention, a composition rich in useful amino acids can be produced with high purity, efficiency, and low cost, while removing harmful heavy metals. it can. This composition can be effectively used, for example, as a seasoning for adding umami to cooked foods, cooking dashi, processed food ingredients, and the like.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
Initially, the manufacturing method of the amino acid composition of this invention is demonstrated.
FIG. 1 is a process diagram showing a method for producing a useful amino acid composition of the present invention. In the figure, the starting material 1 is a portion of seafood to be discarded (hereinafter referred to as a portion of seafood). As shown in the figure, the first step and the second step are performed continuously, and the solid 2 and the liquid phase 3 are obtained from the portion of seafood.
This first step is a step of separating the neutral oil contained in the fish and shellfish tissue as a water-insoluble phase. Specifically, the mixture of the starting material 1 and water is boiled at a predetermined temperature. And heating for a predetermined time. Hereinafter, this first step is referred to as a steaming step in the present invention.
Here, as the starting material 1, it is preferable to use a portion of fish and shellfish that has been conventionally discarded without being effectively used as a by-product produced from the marine fishery processing step. Preferably, scallops that are soft bodies other than scallops or squids that are internal organs of squid can be used.

  The steaming process in the case of using the seafood part as the starting material 1 can be performed by adding 0.5 to 5 times the weight of water to the seafood part and heating to 95 to 100 ° C. . When cooking is performed for a long time, the useful phospholipid, which is the target product in the “useful phospholipid composition and production method thereof” filed on the same date in relation to the present invention, may be decomposed. This useful phospholipid is glycerin phosphate, and examples thereof include phosphatidylcholine and phosphatidylethanolamine. For this reason, when trying to obtain each of the useful phospholipid composition and the useful amino acid composition of the present invention efficiently from the starting material, the cooking time should be set to a short time so that the useful phospholipid is not decomposed. Is desirable.

The second step is a step of separating the solid 2 and the liquid phase 3 after the first step. This step can be performed by a solid-liquid separation step by centrifugation and / or filtration, but a centrifugation step can be preferably used. Specifically, after completion of the first step, the solid material 2 and the liquid phase 3 are obtained by centrifuging at a predetermined rotational speed for a predetermined time. The centrifugal force in this case may be about 1000 to 3000G. From this liquid phase 3, useful phospholipids such as phosphatidylcholine and phosphatidylethanolamine can be obtained. Further, the liquid phase 3 may be recovered as waste.
In addition, since the solid substance 2 obtained by the centrifugation process contains a water | moisture content, in order to reduce a water | moisture content, it is preferable to perform reduced pressure drying and to reduce the water content of a solid substance. In this case, drying under reduced pressure is desirable because atmospheric pressure drying may cause decomposition of the intended useful amino acid composition due to high temperature.

The third step is an organic solvent extraction step of the solid 2 obtained in the second step. In this step, an organic solvent is added to the solid 2 and extraction with the organic solvent is performed to obtain a residual solid 4 and a liquid phase 5. This organic solvent extraction step can be performed, for example, in an explosion-proof tank with a stirrer.
This organic solvent extraction step can be performed, for example, by adding an organic solvent having the same weight as the solid 2 to the solid 2, and this step can be repeated. Moreover, this organic solvent extraction process can also extract continuously with the extraction apparatus of a cylindrical column shape.
Here, methanol, ethanol, propanol, butanol, acetone, chloroform, methylene chloride, hexane and their hydrates can be used as the organic solvent, but ethanol is most preferable in consideration of food use. Ethanol is an organic solvent that is harmless even if a trace amount is taken into the human body and has a performance that can be used efficiently for the purpose of the present invention. The organic solvent can also be hydrous acetone. In this case, the water-containing acetone preferably contains 10 to 30% by weight of water.

The fourth step is a hydrolysis step of the residual solid 4 extracted with the organic solvent obtained in the third step. The polymer protein is decomposed into amino acids by the hydrolysis process. In the hydrolysis step, hydrochloric acid is preferably added in order to ionize Cd (cadmium) and other heavy metals contained in the residual solid 4. According to the hydrolysis step by the addition of hydrochloric acid, heavy metals are ionized and eluted into the aqueous phase, which is suitable for subsequent processing.
Instead of this hydrolysis step, the residual solid 4 is diluted with a hydrochloric acid concentration lower than the above hydrolysis, the hydrolysis time is shortened, and Cd is ionized under mild conditions. May be degraded to low peptide-containing amino acids by proteolytic enzymes.

  The fifth step is a neutralization step of the content after the fourth step which is acidic. Neutralization can be carried out using any ordinary alkaline chemical, but the content is preferably made weakly acidic to neutral by the addition of sodium carbonate powder or an aqueous solution thereof. In the weakly acidic to neutral contents, the contained Cd and other heavy metals become insoluble in water and mostly precipitate.

  The 5A process is a filtration process of the contents after the 5th process, and is separated into the filtrate 6 and the residue 7. At this time, most of Cd and other heavy metals are transferred to the residue 7 by filtration, and the Cd and other heavy metals recovered in the residue 7 are separately treated. Processing is simple.

  The sixth step is a step of extracting amino acids contained in the filtrate 6 obtained in the fifth step. In this step, a solution comprising a useful amino acid composition 12 can be obtained after removing trace impurities and salts other than amino acids contained in the filtrate 6. Moreover, if the solution which consists of useful amino acid compositions is dried, the powdery useful amino acid composition 15 can be obtained.

Instead of this hydrolysis step, the residual solid 4 is diluted with a hydrochloric acid concentration lower than the above hydrolysis, the hydrolysis time is shortened, Cd is ionized under mild conditions, and the high molecular protein in the residual solid is obtained. It may be decomposed into low peptide-containing amino acids by proteolytic enzymes.
Specifically, after the fifth neutralization step, the temperature is adjusted by adding water for dilution. A proteolytic enzyme is added to the temperature-adjusted diluted water, and the enzymatic decomposition is performed for a predetermined time. When a predetermined time has elapsed, the diluted water is heated and further cooled with water to inactivate the proteolytic enzyme. In the subsequent steps, the useful amino acid composition containing the useful peptide can be produced by performing the steps after the 5A step.

FIG. 2 is a process diagram showing further specific steps in the method for producing a useful amino acid composition of the present invention. In the figure, the sixth step is a region surrounded by a dotted line indicated by an arrow.
The 6A step is a pH adjusting step for making the filtrate 6 extracted in the 5A step alkaline. The pH is preferably adjusted to 8.5 to 9.5. Any ordinary alkaline chemical can be used, but sodium hydroxide is preferred.
Next, the filtrate 6 made alkaline after Step 6A is heated (see Step 6B in FIG. 2). Thereby, the organochlorine toxic substance mixed in the filtrate 6 made alkaline can be decomposed.

At this stage, processes for recovering impurities such as heavy metals are Steps 6C to 6E.
First, in the 6C step, the pH of the alkaline aqueous solution 6 in the 6B step is neutralized with an acid such as hydrochloric acid to precipitate insoluble components to obtain a form suitable for the subsequent treatment.
Next, the liquid neutralized in step 6C is filtered and separated into filtrate 8 and filtration residue 9 (see step 6D in FIG. 2).
Next, the ion exchange resin process of the 6E process is performed. Specifically, the filtrate 8 is passed through a vertical cylinder filled with an ion exchange resin such as a chelate resin to obtain an effluent 10. It is desirable to further adjust the pH to weak acidity by adding hydrochloric acid or the like to the effluent 10 in this step. Thereby, harmful heavy metals such as remaining iron and cadmium can be substantially removed. At this stage, the filtrate 8 becomes an almost pure amino acid solution.

Next, a pure amino acid solution 11 can be obtained by removing trace impurities from the effluent 10 obtained in the 6E step by a decolorization step using an adsorbent (see step 6F in FIG. 2). As the adsorbent, activated carbon can be preferably used. Since this amino acid solution 11 contains salt, it can be used as a liquid seasoning in this form.
Next, the amino acid solution 11 is subjected to a drying process in the 6G process, whereby a powder of useful amino acid composition 12 containing salt can be obtained. This drying step can be, for example, a spray drying step.

Next, the desalting process of the effluent 10 will be described.
FIG. 3 is a process diagram showing an example of a desalting step in the method for producing a useful amino acid composition of the present invention. In the figure, the sixth step is a region surrounded by a dotted line indicated by an arrow. Until the useful amino acid solution 11 containing salt is obtained, the process is the same as that shown in FIG.
Next, the salt contained in the salt-containing amino acid solution 11 is removed by an electrodialyzer or the like in the desalting step of the sixth H step.
Next, if necessary, a decolorization step using an adsorbent is performed to obtain a pure amino acid solution 13 containing no salt.
And the powder of the useful amino acid composition 14 in which salt content is not contained can be obtained by performing the drying process of 6th G process for the amino acid solution 13 in which salt content is not included. This drying step can be, for example, a spray drying step.

  According to the present invention, heavy metals such as Cd contained in fish and shellfish can be ionized by addition of hydrochloric acid, concentrated with an ion exchange resin, etc., and almost completely recovered. For this reason, it is possible to prevent the useful amino acid compositions 11 to 14 from containing heavy metals. This makes it possible to efficiently extract a useful amino acid composition that contains abundant amino acids and functional amino acids as umami and sweetness components from previously discarded portions of seafood.

  The useful amino acid composition 14 produced by the present invention comprises 20% by weight or more of aspartic acid and glutamic acid, 30% by weight or more of serine, glycine, threonine and alanine, and 20% by weight or more of arginine. And an amino acid consisting of lysine.

  Moreover, the foodstuff or food compounding agent containing the useful amino acid composition of this invention contains the amino acid composition 11, 12, 13, 14 obtained by said extraction process process. In this case, the liquids 11 and 13 containing salt or a salt-free useful amino acid composition can be used as a liquid seasoning or a raw material for a liquid seasoning. Further, powders 12 and 14 obtained by drying the liquid can also be used. A compounding quantity can be arbitrarily set according to the use to the foodstuff or food compounding agent of these compositions. For example, when used as a seasoning, a salt-containing product is used, and when used as a functional food, a salt-free product may be used.

Hereinafter, the present invention will be described in detail by way of examples.
A useful amino acid solution 11 of Example 1 was obtained using scallops as starting material 1. The scallop weight of the starting material 1 is 1000 kg, and is in a state where it has been drained for a while but not particularly dried (hereinafter referred to as a wet standard). The breakdown is a solid content of 23% by weight and a water content of 770 kg. The protein in the solid content is about 13.5% in the case of scallops.
First, the starting material 1 was continuously added, and the first cooking step and the second solid-liquid separation step were continuously performed. Specifically, 2000 liters of water was added to the starting material 1 and heated and boiled in a steam jacket heating type continuous steaming tube at 95 to 100 ° C. for 20 minutes.

  Subsequently, as a second solid-liquid separation step, the solid substance 2 and the liquid phase 3 were obtained by passing through a continuous centrifugal separator and rotating at 2000 G. This liquid phase 3 was recovered as waste. The weight of the solid 2 obtained in this step was 470 kg, and the water content was 61.7% by weight as measured by Karl Fischer.

  As an organic solvent extraction step, 146 kg (about 55 kg of dry solid content) of the above-mentioned solid material 2 is filled into a stainless steel cylindrical extraction device (diameter 0.5 m, height 1.5 m, capacity 200 liters), The extraction operation was performed by circulating 300 liters of 95% ethanol at a circulation rate of 100 liters / hour. Finally, the residual liquid phase was extruded with air, and this was added to the organic solvent extract 5 to obtain about 400 liters of the organic solvent extract 5. The extraction residue solid 4 obtained at this time was 51 kg.

Next, as a fourth hydrolysis step performed by adding an acid, 51 kg of the extracted residual solid 4 (dry solid content of about 40 kg), 28 liters of hydrochloric acid (322 mol, molar ratio of nitrogen to 1.13), 25 water Hydrolysis was performed by adding 1 liter. In this case, after the start of boiling, the condensed water started hydrolyzing for 11 hours, and the protein in the residual solid 4 was hydrolyzed into amino acids. The condensed water generated at this time was neutralized and discarded.
As a fifth neutralization step, 10 kg (119 mol) of soda ash is added to the above hydrolyzed solution for primary neutralization, and about 31 kg (20% aqueous solution) of caustic soda is added with stirring. The pH was adjusted to 5 to 5.5 and allowed to cool overnight.
And the filtration process of 5A process was performed with the centrifuge, and it isolate | separated into 74 liters of filtrate 6 and the residue 7 of about 14 kg on a dry basis. This residue 9 contained 230 ppm of Cd.

In Steps 6A to 6C, sodium hydroxide is added to adjust the pH to about 9 to 9.2, the mixture is heated at 80 to 83 ° C. for 3 hours, 3N hydrochloric acid is added to adjust the pH to 5.9 to 6 Adjusted to .2.
Next, as Step 6D, about 2 kg of silica filter aid radiolite is added and filtered through a centrifuge, and separated into about 80 liters of filtrate 8 and 4.2 kg of residue 9 on a dry basis. did. This residue 9 contained 1400 ppm of Cd, and it was found that a significant concentration effect was obtained.
Next, as a 6E step, the filtrate 8 was treated with a chelate resin column (Diaion CR-11, manufactured by Mitsubishi Chemical Corporation), and about 110 liters of the effluent 10 was obtained by water extrusion. When the effluent 10 was subjected to metal analysis using an emission spectrometer, heavy metals such as iron and cadmium were not detected within the detection limit range.
At this time, the recovered liquid obtained by regenerating sulfuric acid through the chelate resin column after Step 6E and neutralizing the effluent sulfuric acid with caustic soda contained a trace amount of Cd of 0.09 ppm. The recovered ash and the residues 9 and 11 after the filtration were incinerated, and the remaining ash was transferred to a Cd processor.

  Next, about 2.5 liters of hydrochloric acid is added to the effluent 10 to adjust the pH to 4.9 to 5.0, about 3 kg of activated carbon is added, stirred, separated and decolored, about 90 liters. A useful amino acid solution 11 containing sodium chloride was extracted. This amino acid solution 11 was almost colorless and transparent.

  As Example 2, about 90 liters of amino acid solution 11 obtained in Example 1 was spray-dried. As a result, a powdery useful amino acid composition 12 containing 33.4 kg of sodium chloride was obtained.

  The useful amino acid solution 11 containing sodium chloride obtained in Example 1 was dechlorinated. About 90 liters of useful amino acid solution 11 was applied to an ion exchange membrane electrodialyzer (manufactured by Nippon Nurisui Co., Ltd., DW-1 type) to remove residual salt, and further subjected to decolorization filtration. Thereby, as Example 3, 75 liters of solution containing the salt-free useful amino acid composition 13 was obtained.

  The process up to Step 6F obtained in Example 3 is the same as that in Example 1. However, Step 6G is further performed, and 75 liters of a solution containing salt-free useful amino acid composition 13 is spray-dried. A powdered useful amino acid composition 14 (about 28 kg) was obtained.

The solid residue 4 was produced in the same manner as in Example 1, and the useful amino acid composition 14 of Example 5 was produced using a proteolytic enzyme by the following steps.
First, 12.3 liters of hydrochloric acid and 130 liters of water were added to about 51 kg of the extraction residue solid 4 (dry solid content of about 40 kg), and primary decomposition was performed for 3 hours after the start of boiling. Next, as a fifth neutralization step, ca. 31 kg (20%) of caustic soda is added to the above solution with stirring, the pH in the system is adjusted to 7-7.2, and 65 liters of water is added to about 270 liters of water. Diluted solution. The diluted solution was adjusted to 40 to 43 ° C., and 25 g of peptidase R (manufactured by Amano Enzyme) and horseamizyme (manufactured by Amano Enzyme) were added as proteolytic enzymes to perform enzymatic degradation for 24 hours. Thereafter, the mixture was heated to 90 ° C. and further cooled with water to inactivate the proteolytic enzyme.
Next, 5 kg of pearlite and 3 kg of radiolite silica filter aid radiolite were added to this diluted solution, filtered, washed with water, and separated into about 190 liter of filtrate 6 and 26 kg of residue 7 on a dry basis. This residue 7 contains about 230 ppm of Cd, and it has been found that a significant concentration effect can be obtained.
Next, the filtrate 6 was treated with a chelate resin column (Diaion CR-11, manufactured by Mitsubishi Chemical Corporation), and about 190 liters of the effluent 10 was obtained by water extrusion. When the effluent 10 was subjected to metal analysis using an emission spectrometer, heavy metals such as iron and cadmium were not detected within the detection limit range.
At this time, the recovered liquid obtained by regenerating sulfuric acid through the chelate resin column after Step 6E and neutralizing the effluent sulfuric acid with caustic soda contained 0.3 ppm of trace amount Cd. The recovered ash and the residues 9 and 11 after the filtration were incinerated, and the remaining ash was transferred to a Cd processor.

  Next, about 0.76 liters of hydrochloric acid is added to the effluent 10 to adjust the pH from 5.3 to 5.5, and it remains on an ion exchange membrane electrodialyzer (made by Nippon Nersui, DW-1 type). The salt content was removed, and about 140 liters of a solution 13 containing a useful amino acid composition not containing sodium chloride was obtained. Further, it was subjected to decolorization filtration and spray-dried to obtain a powdery useful amino acid composition 14 (about 18 kg) of Example 5. This useful amino acid composition 14 contained a useful peptide.

実施例１の有用アミノ酸組成物１４の１００ｇ中には、アスパラギン酸８．４５ｇ、グルタミン酸１２．５７ｇ、セリン７．９２ｇ、グリシン７．９２ｇ、ヒスチジン１．９０ｇ、アルギニン１０．０６ｇ、スレオニン８．２３ｇ、アラニン８．３０ｇ、プロリン６．６３ｇ、チロジン０．５３ｇ、バリン４．８０ｇ、メチオニン３．１２ｇ、イソロイシン２．２１ｇ、ロイシン４．８０ｇ、フェニルアラニン２．５１ｇ、リジン１０．０５が含まれていた。表１中には、比較のため同じ方法で分析した、いわし魚粉、大豆蛋白、馬鈴薯蛋白からのアミノ酸組成物分析結果も示した。 Table 1 shows the composition analysis results of the useful amino acid composition 14 obtained in Example 4.

In 100 g of the useful amino acid composition 14 of Example 1, 8.45 g of aspartic acid, 12.57 g of glutamic acid, 7.92 g of serine, 7.92 g of glycine, 1.90 g of histidine, 10.06 g of arginine, and 8.23 g of threonine. , Alanine 8.30 g, proline 6.63 g, tyrosin 0.53 g, valine 4.80 g, methionine 3.12 g, isoleucine 2.21 g, leucine 4.80 g, phenylalanine 2.51 g, lysine 10.05. . Table 1 also shows the results of amino acid composition analysis from sardine fish meal, soybean protein, and potato protein, which were analyzed by the same method for comparison.

As shown in Table 2, among the amino acids contained in the useful amino acid composition 14 of Example 1, aspartic acid and glutamic acid are known as amino acids related to umami as seasonings, and the amount was 21.02 g. It was. This value is smaller than soy protein and potato protein, but more than sardine fish meal.
Moreover, serine, glycine, threonine, and alanine are amino acids related to sweetness, and the amount thereof was 32.37 g. This value was almost the same as sardine fish meal, and was found to be larger than soy protein and potato protein.
Furthermore, arginine and lysine are known as various highly functional amino acids, and the amount thereof was 20.11 g. This value was found to be larger than any of the comparative examples.
Thus, the useful amino acid composition 14 of Example 1 has a higher content of functional amino acids than sardine fish meal, which is a comparative example derived from the same seafood, and contains amino acids related to umami and sweetness to the same extent. There was found. Therefore, the useful amino acid composition 14 of Example 1 extracted from scallops contains abundant functional amino acids and amino acids related to umami and sweetness.

  When the powdered useful amino acid composition 14 obtained in Example 1 was used as a seasoning for cooking, it was well received by tasters for adding a mild umami and sweetness to the cooked product.

  The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope of the invention described in the claims, and it goes without saying that these are also included in the scope of the present invention. . For example, the pH in the above process, the type of ion exchange resin used for heavy metal removal, and the like can be designed as appropriate, and it goes without saying that the seafood site is not limited to scallops or squid.

It is process drawing which shows the manufacturing method of the useful amino acid composition derived from seafood by this invention. It is process drawing which shows the further specific process in the manufacturing method of the useful amino acid composition of this invention. It is process drawing which shows an example of the desalting process in the manufacturing method of the useful amino acid composition of this invention.

Explanation of symbols

1: Starting material 2: Solid matter 3, 5: Liquid phase 4: Residual solid matter 6, 8: Filtrate 7, 9: Residue 10: Effluent 11: Salt-containing useful amino acid solution 12: Salt-containing useful amino acid composition 13 : Salt-free amino acid solution 14: Useful amino acid composition

Claims (9)

First and second steps for removing moisture and oil from a portion of seafood and taking out a solid,
A third step of adding an organic solvent to the solid, separating and removing the organic solvent extract, and taking out the residual solid;
A fourth step of adding an acid to the residual solid to hydrolyze,
A fifth step of neutralizing and filtering after the hydrolysis to obtain a filtrate;
A sixth step of extracting a useful amino acid composition from an effluent obtained by removing heavy metals from the filtrate;
A method for producing a useful amino acid composition, comprising:       The first and second steps consist of a steaming step in which water is added to the portion of the seafood and steamed at a predetermined temperature for a predetermined time, and a solid-liquid separation step by centrifugation and / or filtration, and the steaming step and The method for producing a useful amino acid composition according to claim 1, wherein the solid-liquid separation step is continuously performed.       The method for producing a useful amino acid composition according to claim 1, wherein the organic solvent is ethanol or water-containing acetone.       The method for producing a useful amino acid composition according to claim 1, wherein in the fourth step, hydrolysis is performed by adding hydrochloric acid, and the filtrate is passed through an ion exchange resin to remove heavy metals. .       The useful amino acid composition according to claim 1, wherein, in the fourth step, a useful amino acid composition containing a useful peptide is obtained by adding a proteolytic enzyme after hydrolysis and further performing enzymatic degradation. Production method.       In the sixth step, the filtrate is adjusted to be alkaline and then heated. Further, the pH of the filtrate is acidified, the filtrate is filtered to obtain a filtrate and a residue, and heavy metals are removed from the filtrate. The method for producing a useful amino acid composition according to claim 1, wherein the effluent is obtained, the effluent is decolorized and purified, and the salt content is removed to extract the useful amino acid composition.       The method for producing a useful amino acid composition according to claim 6, wherein after the salt content is removed in the sixth step, a powdery useful amino acid composition is obtained by a drying step.       The method for producing a useful amino acid composition according to claim 1, wherein the seafood part is a by-product produced from a fish processing process.       The method for producing a useful amino acid composition according to claim 8, wherein the by-product is scallop uro.

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