CN102318725B - Feed with chicken viscera as raw materials and preparation method thereof - Google Patents
Feed with chicken viscera as raw materials and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of animal feed; the invention discloses feed with chicken viscera as raw materials and a preparation method thereof; the feed comprises 30%-36% by weight of polypeptides and 35%-66% by weight of amino acids. The amino acids in the feed have 17 types; and the digestion and absorption rate of the feed is up to above 90%; according to the invention, the feed is obtained by process steps of enzymatic hydrolysis of various protease, fat removal by organic solvents, and ultrafiltration; the method of the invention has the advantages of simple process, high finished product yield, and suitability for industrial production.
Description
Technical field
The invention belongs to technical field of animal, be specifically related to a kind of raw material and be feed that chicken organ prepares and preparation method thereof.
Chicken organ of the present invention comprises heart, liver, lung, kidney, intestines of animal chicken etc., the internal organ part of namely removing behind the slaughtering chickens.
Background technology
Chicken organ is the internal organ of Phasianidae animal man chicken, and shy stink is arranged.Chicken organ has following purposes: (1) is prepared into typical local food as the raw material of smoking food, and is edible for people; (2) use as bait; (3) use as feed: the feeds such as feeding fish, tortoise, soft-shelled turtle use, and perhaps the feedstuff as domestic pets cat or dog uses; Etc..
Along with greatly developing of edible chicken aquaculture, the output annual increment amount of chicken organ reaches more than 10%, and therefore, the deep development of chicken organ will solve the deep development problem in edible chicken slaughterhouse; Chicken organ is as the feedstuff of domestic pets cat and dog, be widely used, but most of feed factory is very simple to the processing mode of chicken organ: by using as feed after cleaning, pulverizing, the drying, this processing chicken organ method has very large shortcoming: (1) chicken organ processing mode is simple, some bacteriums, virus also are retained in the raw material, cat or dog food are easy to cause the generation of parasite or disease after using; (2) contain a large amount of stodgy materials and useless material in the chicken organ, behind the animal edible, increased the weight of Alimentary burden, cause for a long time the disease of animal digestion organ; (3) active ingredient can't play a role in the chicken organ, particularly contains immune protein, amino acid etc. in the chicken organ, can not give full play to its effect, causes the waste of resource.Therefore, chicken organ further be studied when using as feedstuff.
Chinese patent application number is the production technology of the disclosed chicken intestinal peptide of 200910099838.x, this technique has obtained containing the chicken intestinal peptide of the materials such as 3-methyl indol and amino acid by methods such as enzymolysis, thereby can eat feed by induced animal because contain the 3-methyl indol, although this patent has been carried out deep exploitation to the chicken intestines, but have following shortcoming: the composition in (1) chicken organ is lost, contain amino acid/11 in the chicken organ more than 5 kinds, but amino acid only have 10 kinds in the chicken intestinal peptide that the method obtains; Contain important active ingredient-polypeptide in the chicken organ, this process can't obtain; (2) add chemical composition: this process has added chemical composition antioxidant BHA in order to keep 3-methyl indol content, and there is potential danger in animal safety; (3) invalid components is not removed, and such as the animal tallow constituents, this constituents is for pet cat and pet dog, and is nondigestible, and can bring some diseases.
Summary of the invention
For these reasons, the inventor obtains the feed that a kind of new chicken organ prepares by experimental study, contains polypeptide and amino acid in this feed, and wherein the amino acid kind reaches 17 kinds; The digestibility of this feed can reach more than 90%; The present invention removes the different process methods such as fat, ultrafiltration, ion-exchange by enzymolysis, the organic solvent of different protease, obtains above-mentioned feed, and the method has the advantages such as technique is simple, high, the suitable suitability for industrialized production of finished product yield.
The present invention is achieved through the following technical solutions.
A kind of chicken organ is the feed of raw material, and feed contains polypeptide weight content 30%-36%, amino acid weight content 35%-66%.
The digestibility of above-mentioned feed is greater than 90% and less than 100%.
Amino acid weight percentage in the above-mentioned feed is respectively aspartic acid 0.5-1.5%, glutamic acid 3.0%-11%, threonine 1.0%-3.0%, arginase 12 .0%-3.0%, proline 2.0%-4.0%, valine 0.02%-0.05%, isoleucine 2.0%-3.0%, phenylalanine 2.0%-3.5%, lysine 3.0%-6.0%, serine 2.0%-3.5%, glycine 2.0%-3.0% histidine 1.5%-2.0%, alanine 4.0%-6.0%, cystine 1.0%-1.5%, methionine 5.0%-7.0%, leucine 3.0%-6.0%, tyrosine 1.5%-2.5%.
The amino acid detection method is measured according to GB/T18246-2000 in the feed of the present invention.
The preparation method of above-mentioned feed includes but not limited to following: get the chicken organ that cleans up, add pepsin or trypsase under 20-80 ℃ of condition, stir, be incubated 2-5 hour, add organic solvent, mix, left standstill 1-3 hour, separate clear liquid, filter, filtrate is through the ultrafiltration of molecular cut off 5000-8000 milipore filter, keep and see through liquid, concentrated, drying, and get final product.
The preparation method of above-mentioned feed includes but not limited to following: the chicken organ that cleans up, under 20-80 ℃ of condition, add papain, and stir, be incubated 2-5 hour, add organic solvent, mix, left standstill 1-3 hour, separate clear liquid, filter, filtrate is through the ultrafiltration of molecular cut off 5000-8000 milipore filter, keep and see through liquid, concentrated, drying, and get final product.
The preparation method of above-mentioned feed includes but not limited to following: get the chicken organ that cleans up, add pepsin or papain under 20-80 ℃ of condition, stir, be incubated 2-5 hour, add the machine solvent, mix, left standstill 1-3 hour, separate clear liquid, under 20-80 ℃ of condition, add trypsase, insulation 2-5h, filter, concentrated, drying, and get final product.
The preparation method of above-mentioned feed includes but not limited to following: get the chicken organ that cleans up, add pepsin or papain under 20-80 ℃ of condition, stir, be incubated 2-5 hour, add the machine solvent, mix, left standstill 1-3 hour, and separated clear liquid, filter, the strong-acid type cation exchange column washes with water on the filtrate, and eluent discards, be the ammoniacal liquor wash-out of 5%-25% with concentration, collect eluent, concentrated, remove ammonia to most, drying, and get final product.
Organic solvent includes but not limited to following among the above-mentioned preparation method: carbon tetrachloride, benzinum, ethanol, ether, carrene, chloroform, methyl alcohol, normal propyl alcohol, isopropyl alcohol, acetone, butanone, espeleton, methylisobutylketone, n-hexane, pentane, oxolane, ethyl acetate, propyl acetate, butyl acetate etc.
Feed of the present invention can be used as one of raw material of animal feed and uses.
The dietary protein origin that the purpose of this invention is to provide animal feed.
The above-mentioned protease of the present invention includes but not limited to that said method mentions, and can use other protease that can make the animal protein enzymolysis.
Feed of the present invention have a following advantages:
1, strengthens the innate immunity ability of animal
Contain the materials such as polypeptide, behind the animal edible, can excite its innate immunity ability, prevent the generation of disease.
2, guarantee the intestines problems such as digestion security, prevention diarrhoea
The material that this feed contains is the material of molecular weight below 8000, is easy to digesting and assimilating of animal, avoids increasing the weight of the digestion burden of animal, can prevent the intestines problems such as diarrhoea.
3, reduce ight soil peculiar smell and faecal volume
This feed does not contain the stodgy materials such as animal protein and animal tallow, thereby can reduce the ight soil peculiar smell; The digestibility of feed of the present invention can reach more than 90%, thereby has reduced the volume of ight soil.
One, the mensuration of digestibility
Experimental animal: (about 2 years old) the male Poodle that grows up, 22 kilograms of body weight, totally 4.
Test feed: the feed that the embodiment of the invention 1, embodiment 10, embodiment 19, embodiment 22 obtain.
Test method: 4 normal nursings for 1 week of Poodle, measure the normal amount of every dog, do not find to exist disease to continue test; Front 24 hours of on-test, do not give any food, only gives drinking water; After 24 hours, begin test, the feed (giving respectively the feed of different embodiment) of the amount that every Poodle is measured, freely drink water, adopt the full fecaluria method of receiving, collect 24 hours fecalurias, adopt micro-Kjeldahl to measure the nitrogen content that gives food, and the nitrogen content in the fecaluria of collecting, calculate according to the following equation digestibility: (food nitrogen content-fecaluria nitrogen content)/food nitrogen content * 100%.
Result of the test: see Table 1.
Table 1 feed digestibility of the present invention
Conclusion (of pressure testing): above-mentioned test shows that the digestibility of feed of the present invention is more than 90%, the highlyest can reach 98%, proves absolutely that the present invention has scientific meaning.
Two, Preparation Example
Embodiment 1
Get 10 kilograms of the chicken organs that clean up, under 40 ℃ of conditions, add pepsin, stir, be incubated 3 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 6000 milipore filter ultrafiltration, and is concentrated, drying namely gets 6.43 kilograms in feed.
Contain in the above-mentioned feed: polypeptide weight content 35.5%, amino acid weight content 60.84%.Aspartic acid 1.3%, glutamic acid 10.4%, threonine 2.9%, arginase 12 .4%, proline 3 .1%, valine 0.04%, isoleucine 3.0%, phenylalanine-3,4-quinone .2%, lysine 5.7%, serine 3.3%, glycine 2.6% histidine 1.8%, alanine 5.7%, cystine 1.4%, methionine 6.8%, leucine 5.2%, tyrosine 2.0%.
Embodiment 2
Get 3 kilograms of the chicken organs that clean up, under 25 ℃ of conditions, add pepsin, stir, be incubated 2 hours, add machine solvent carbon tetrachloride, mix, left standstill 2 hours, and separated clear liquid (carbon tetrachloride layer reclaims solvent to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 1.88 kilograms in feed.
Contain in the above-mentioned feed: polypeptide weight content 31.3%, amino acid weight content 58.604%.Aspartic acid 1.2% in the amino acid, glutamic acid 10.0%, threonine 2.55%, arginase 12 .1%, proline 3 .03%, valine 0.024%, isoleucine 2.91%, phenylalanine-3,4-quinone .11%, lysine 5.63%, serine 3.21%, glycine 2.43% histidine 1.72%, alanine 5.61%, cystine 1.30%, methionine 6.72%, leucine 5.13%, tyrosine 1.93%.
Embodiment 3
Get 1 kilogram of the chicken organ that cleans up, under 50 ℃ of conditions, add pepsin, stir, be incubated 5 hours, add machine solvent benzinum, mix, left standstill 3 hours, and separated clear liquid (petroleum ether layer reclaims benzinum to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.625 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 34.7%, amino acid weight content 59.93%, aspartic acid 1.25%, glutamic acid 10.34%, threonine 2.91%, arginase 12 .25%, proline 3 .03%, valine 0.03%, isoleucine 2.96%, phenylalanine-3,4-quinone .13%, lysine 5.67%, serine 3.28%, glycine 2.50%, histidine 1.73%, alanine 5.62%, cystine 1.40%, methionine 6.71%, leucine 5.15%, tyrosine 1.97%.
Embodiment 4
Get 1 kilogram of the chicken organ that cleans up, under 45 ℃ of conditions, add trypsase, stir, be incubated 2.5 hours, add the machine solvent methanol, mix, left standstill 2 hours, and separated clear liquid (methanol layer reclaims methyl alcohol to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.614 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 35.6%, amino acid weight content 59.115%; Aspartic acid 1.23%, glutamic acid 10.2%, threonine 2.6%, arginase 12 .2%, proline 3 .13%, valine 0.025%, isoleucine 2.92%, phenylalanine-3,4-quinone .14%, lysine 5.65%, serine 3.21%, glycine 2.43% histidine 1.71%, alanine 5.6%, cystine 1.32%, methionine 6.74%, leucine 5.11%, tyrosine 1.90%.
Embodiment 5
Get 1 kilogram of the chicken organ that cleans up, under 35 ℃ of conditions, add pepsin, stir, be incubated 2.5 hours, add the machine methylene chloride, mix, left standstill 1.5 hours, and separated clear liquid (dichloromethane layer reclaims solvent to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 8000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.604 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 32.5%, amino acid weight content 59.011%.Aspartic acid 1.29% in the amino acid, glutamic acid 10.1%, threonine 2.53%, arginase 12 .2%, proline 3 .05%, valine 0.021%, isoleucine 2.94%, phenylalanine-3,4-quinone .13%, lysine 5.62%, serine 3.27%, glycine 2.43% histidine 1.73%, alanine 5.61%, cystine 1.32%, methionine 6.72%, leucine 5.11%, tyrosine 1.94%.
Embodiment 6
Get 1 kilogram of the chicken organ that cleans up, under 40 ℃ of conditions, add trypsase, stir, be incubated 4 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.608 kilogram in feed.
Above-mentioned feed contains: polypeptide weight content 35%, amino acid weight content 59.075%.Aspartic acid 1.21%, glutamic acid 10.2%, threonine 2.6%, arginase 12 .2%, proline 3 .13%, valine 0.025%, isoleucine 2.92%, phenylalanine-3,4-quinone .14%, lysine 5.62%, serine 3.21%, glycine 2.42% histidine 1.73%, alanine 5.6%, cystine 1.32%, methionine 6.74%, leucine 5.11%, tyrosine 1.90%.
Embodiment 7
Get 1 kilogram of the chicken organ that cleans up, under 30 ℃ of conditions, add pepsin, stir, be incubated 2 hours, add the machine solvent ethyl acetate, mix, left standstill 1 hour, separate clear liquid, filter, filtrate is through molecular cut off 5000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.576 kilogram in feed of the present invention.
Above-mentioned feed contains: polypeptide weight content 30.8%, amino acid weight content 35.57%.Weight percentage is respectively aspartic acid 0.55% in the amino acid, glutamic acid 3%, threonine 1.0%, arginase 12 .0%, proline 2.0%, valine 0.02%, isoleucine 2.0%, phenylalanine 2.0%, lysine 3.0%, serine 2.0%, glycine 2.0% histidine 1.5%, alanine 4.0%, cystine 1.0%, methionine 5.0%, leucine 3.0%, tyrosine 1.5%.
Embodiment 8
Get 1 kilogram of the chicken organ that cleans up, under 50 ℃ of conditions, add trypsase, stir, be incubated 5 hours, add organic solvent ethanol, mix, left standstill 3 hours, separate clear liquid, filter, filtrate is through molecular cut off 8000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.619 kilogram in feed of the present invention.
Contain in the above-mentioned feed: polypeptide weight content 30.3%, amino acid weight content 36.42%.Weight percentage is respectively aspartic acid 0.55% in the amino acid, glutamic acid 3.10%, threonine 1.05%, arginase 12 .1%, proline 2.1%, valine 0.02%, isoleucine 2.1%, phenylalanine 2.0%, lysine 3.0%, serine 2.1%, glycine 2.1% histidine 1.5%, alanine 4.0%, cystine 1.0%, methionine 5.1%, leucine 3.0%, tyrosine 1.6%.
Embodiment 9
Get 1 kilogram of the chicken organ that cleans up, under 45 ℃ of conditions, add pepsin, stir, be incubated 3.5 hours, add organic solvent-acetone, mix, left standstill 2.5 hours, separate clear liquid, filter, filtrate is through molecular cut off 7000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.670 kilogram in feed of the present invention.
Contain in the above-mentioned feed: polypeptide weight content 34%, amino acid weight content 53.39%.Wherein weight percentage is respectively aspartic acid 0.95% in the amino acid, glutamic acid 9.3%, threonine 1.75%, arginase 12 .45%, proline 3 .1%, valine 0.04%, isoleucine 2.55%, phenylalanine 2.75%, lysine 4.6%, serine 2.85%, glycine 2.45% histidine 1.75%, alanine 4.85%, cystine 1.35%, methionine 6.25%, leucine 4.6%, tyrosine 1.8%.
Embodiment 10
Get 10 kilograms of the chicken organs that clean up, under 75 ℃ of conditions, add papain, stir, be incubated 3 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 6.37 kilograms in feed.
Contain in the above-mentioned feed: polypeptide weight content 33.5%, amino acid weight content 53.87%.Weight percentage is respectively aspartic acid 0.9% in the amino acid, glutamic acid 7.5%, threonine 2.3%, arginase 12 .43%, proline 3 .1%, valine 0.04%, isoleucine 2.6%, phenylalanine-3,4-quinone .1%, lysine 5.2%, serine 2.8%, glycine 2.7% histidine 1.9%, alanine 5.2%, cystine 1.3%, methionine 5.9%, leucine 4.8%, tyrosine 2.1%.
Embodiment 11
Get 3 kilograms of the chicken organs that clean up, under 55 ℃ of conditions, add papain, stir, be incubated 2 hours, add machine solvent carbon tetrachloride, mix, left standstill 1.5 hours, and separated clear liquid (carbon tetrachloride layer reclaims solvent to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 8000 milipore filter ultrafiltration, and is concentrated, drying namely gets 1.69 kilograms in feed.
Contain in the above-mentioned feed: polypeptide weight content 30.9%, amino acid weight content 36.75%; Weight percentage is respectively aspartic acid 0.52% in the amino acid, glutamic acid 3.1%, threonine 1.2%, arginase 12 .0%, proline 2.1%, valine 0.03%, isoleucine 2.1%, phenylalanine 2.2%, lysine 3.0%, serine 2.1%, glycine 2.0% histidine 1.5%, alanine 4.1%, cystine 1.1%, methionine 5.0%, leucine 3.1%, tyrosine 1.6%.
Embodiment 12
Get 1 kilogram of the chicken organ that cleans up, under 55 ℃ of conditions, add papain, stir, be incubated 5 hours, add machine solvent benzinum, mix, left standstill 3 hours, and separated clear liquid (petroleum ether layer reclaims benzinum to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.613 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 35.04%, amino acid weight content 64.75%; Weight percentage is respectively aspartic acid 1.45% in the amino acid, glutamic acid 10.30%, threonine 2.85%, arginase 12 .90%, proline 3 .85%, valine 0.05%, isoleucine 2.95%, phenylalanine-3,4-quinone .45%, lysine 5.90%, serine 3.40%, glycine 2.90% histidine 1.95%, alanine 6.00%, cystine 1.50%, methionine 6.95%, leucine 5.90%, tyrosine 2.45%.
Embodiment 13
Get 1 kilogram of the chicken organ that cleans up, under 45 ℃ of conditions, add papain, stir, be incubated 2.5 hours, add the machine solvent methanol, mix, left standstill 2 hours, and separated clear liquid (methanol layer reclaims methyl alcohol to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 6000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.624 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 35.3%, amino acid weight content 59.145%.Aspartic acid 1.20%, glutamic acid 10.1%, threonine 2.60%, arginase 12 .20%, proline 3 .10%, valine 0.025%, isoleucine 2.92%, phenylalanine-3,4-quinone .15%, lysine 5.65%, serine 3.20%, glycine 2.45% histidine 1.75%, alanine 5.65%, cystine 1.35%, methionine 6.75%, leucine 5.15%, tyrosine 1.90%.
Embodiment 14
Get 1 kilogram of the chicken organ that cleans up, under 55 ℃ of conditions, add papain, stir, be incubated 2.5 hours, add the machine methylene chloride, mix, left standstill 1.5 hours, and separated clear liquid (dichloromethane layer reclaims solvent to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 8000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.624 kilogram in feed.
Contain in the above-mentioned feed: polypeptide weight content 32.8%, amino acid weight content 58.94%.Aspartic acid 1.30% in the amino acid, glutamic acid 10.1%, threonine 2.53%, arginase 12 .2%, proline 3 .05%, valine 0.020%, isoleucine 2.95%, phenylalanine-3,4-quinone .14%, lysine 5.62%, serine 3.27%, glycine 2.43% histidine 1.73%, alanine 5.60%, cystine 1.30%, methionine 6.75%, leucine 5.10%, tyrosine 1.94%.
Embodiment 15
Get 1 kilogram of the chicken organ that cleans up, under 50 ℃ of conditions, add papain, stir, be incubated 4 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), filter, filtrate keeps seeing through liquid through molecular cut off 5000 milipore filter ultrafiltration, and is concentrated, drying namely gets 0.635 kilogram in feed.
Above-mentioned feed contains: polypeptide weight content 34.3%, amino acid weight content 59.065%.Aspartic acid 1.25%, glutamic acid 10.1%, threonine 2.65%, arginase 12 .23%, proline 3 .10%, valine 0.035%, isoleucine 2.90%, phenylalanine-3,4-quinone .15%, lysine 5.62%, serine 3.21%, glycine 2.42% histidine 1.73%, alanine 5.6%, cystine 1.32%, methionine 6.74%, leucine 5.11%, tyrosine 1.90%.
Embodiment 16
Get 1 kilogram of the chicken organ that cleans up, under 60 ℃ of conditions, add papain, stir, be incubated 2 hours, add the machine solvent chloroform, mix, left standstill 1 hour, separate clear liquid, filter, filtrate is through molecular cut off 5000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.594 kilogram in feed of the present invention.
Above-mentioned feed contains: polypeptide weight content 32.8%, amino acid weight content 35.77%.Weight percentage is respectively aspartic acid 0.65% in the amino acid, glutamic acid 3.10%, threonine 1.0%, arginase 12 .0%, proline 2.0%, valine 0.02%, isoleucine 2.0%, phenylalanine 2.0%, lysine 3.0%, serine 2.0%, glycine 2.0% histidine 1.5%, alanine 4.0%, cystine 1.0%, methionine 5.0%, leucine 3.0%, tyrosine 1.5%.
Embodiment 17
Get the chicken organ that cleans up, under 50 ℃ of conditions, add papain, stir, be incubated 5 hours, add organic solvent ethanol, mix, left standstill 3 hours, separate clear liquid, filter, filtrate is through molecular cut off 8000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.613 kilogram in feed of the present invention.
Contain in the above-mentioned feed: polypeptide weight content 30.2%, amino acid weight content 36.47%.Weight percentage is respectively aspartic acid 0.50% in the amino acid, glutamic acid 3.05%, threonine 1.1%, arginase 12 .1%, proline 2.2%, valine 0.02%, isoleucine 2.2%, phenylalanine 2.0%, lysine 3.0%, serine 2.1%, glycine 2.0% histidine 1.5%, alanine 4.0%, cystine 1.0%, methionine 5.2%, leucine 3.0%, tyrosine 1.5%.
Embodiment 18
Get 1 kilogram of the chicken organ that cleans up, under 55 ℃ of conditions, add papain, stir, be incubated 3.5 hours, add organic solvent methyl alcohol, mix, left standstill 2.5 hours, separate clear liquid, filter, filtrate is through molecular cut off 7000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 0.664 kilogram in feed of the present invention.
Contain in the above-mentioned feed: polypeptide weight content 34%, amino acid weight content 52.34%.Wherein weight percentage is respectively aspartic acid 0.9% in the amino acid, glutamic acid 9.1%, threonine 1.7%, arginase 12 .4%, proline 3 .1%, valine 0.04%, isoleucine 2.6%, phenylalanine 2.8%, lysine 4.7%, serine 2.9%, glycine 2.4% histidine 1.7%, alanine 4.8%, cystine 1.3%, methionine 6.2%, leucine 4.7%, tyrosine 1.7%.
Embodiment 19
Get 10 kilograms of the chicken organs that clean up, under 40 ℃ of conditions, add pepsin, stir, be incubated 3 hours, add machine solvent n-hexane, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), under 40 ℃ of conditions, add trypsase, insulation 3h filters, and is concentrated, drying namely gets 4.93 kilograms in feed.
Feed contains: polypeptide weight content 33.5%, amino acid weight content 53.73%.Weight percentage is respectively aspartic acid 1.35% in the amino acid, glutamic acid 7.5%, threonine 2.45%, arginase 12 .6%, proline 2.85%, valine 0.03%, isoleucine 2.55%, phenylalanine 2.95%, lysine 5.05%, serine 2.95%, glycine 2.45% histidine 1.70%, alanine 5.25%, cystine 1.20%, methionine 5.90%, leucine 5.05%, tyrosine 1.90%.
Embodiment 20
Get 3 kilograms of the chicken organs that clean up, under 30 ℃ of conditions, add pepsin, stir, be incubated 2 hours, add machine solvent carbon tetrachloride, mix, left standstill 2 hours, and separated clear liquid (carbon tetrachloride layer reclaims solvent to most, and residue discards), under 30 ℃ of conditions, add trypsase, insulation 2h filters, and is concentrated, drying namely gets 1.46 kilograms in feed.
Feed contains: polypeptide weight content 30.2%, amino acid weight content 42.625%.Weight percentage is respectively aspartic acid 1.1% in the amino acid, glutamic acid 5.2%, threonine 1.2%, arginase 12 .2%, proline 2.1%, valine 0.025%, isoleucine 2.1%, phenylalanine 2.0%, lysine 4.1%, serine 2.2%, glycine 2.2% histidine 1.6%, alanine 4.1%, cystine 1.1%, methionine 5.6%, leucine 4.2%, tyrosine 1.6%.
Embodiment 21
Get 1 kilogram of the chicken organ that cleans up, under 50 ℃ of conditions, add papain, stir, be incubated 5 hours, add machine solvent benzinum, mix, left standstill 3 hours, and separated clear liquid (petroleum ether layer reclaims benzinum to most, and residue discards), under 45 ℃ of conditions, add trypsase, insulation 4.5h filters, and is concentrated, drying namely gets 0.527 kilogram in feed.
Polypeptide weight content 35.6% in the feed, amino acid weight content 63.34%, weight percentage is respectively aspartic acid 1.45% in the amino acid, glutamic acid 10.5%, threonine 2.9%, arginase 12 .85%, proline 3 .85%, valine 0.04%, isoleucine 2.80%, phenylalanine-3,4-quinone .4%, lysine 5.85%, serine 3.45%, glycine 2.9% histidine 1.95%, alanine 5.50%, cystine 1.45%, methionine 6.75%, leucine 5.25%, tyrosine 2.45%.
Embodiment 22
Get 1 kilogram of the chicken organ that cleans up, under 45 ℃ of conditions, add pepsin, stir, be incubated 2.5 hours, add the machine solvent methanol, mix, left standstill 2 hours, and separated clear liquid (methanol layer reclaims methyl alcohol to most, and residue discards), under 40 ℃ of conditions, add trypsase, insulation 3h filters, and is concentrated, drying namely gets 0.510 kilogram in feed.
Feed contains: polypeptide weight content 35.4%, amino acid weight content 61.15%.Weight percentage is respectively aspartic acid 1.5% in the amino acid, glutamic acid 10.9%, threonine 2.85%, arginase 12 .95%, proline 3 .85%, valine 0.05%, isoleucine 2.90%, phenylalanine-3,4-quinone .45%, lysine 5.75%, serine 3.15%, glycine 2.7% histidine 1.8%, alanine 4.9%, cystine 1.4%, methionine 5.9%, leucine 4.8%, tyrosine 2.3%.
Embodiment 23
Get 1 kilogram of the chicken chicken organ that cleans up, under 55 ℃ of conditions, add papain, stir, be incubated 2.5 hours, add the machine methylene chloride, mix, left standstill 1.5 hours, and separated clear liquid (dichloromethane layer reclaims solvent to most, and residue discards), under 45 ℃ of conditions, add trypsase, insulation 2.5h filters, and is concentrated, drying namely gets 0.535 kilogram in feed.
Feed contains: polypeptide weight content 32.4%, amino acid weight content 49.83%.Weight percentage is respectively aspartic acid 1.2% in the amino acid, glutamic acid 6.8%, threonine 2.2%, arginase 12 .3%, proline 2.7%, valine 0.03%, isoleucine 2.4%, phenylalanine 2.6%, lysine 4.5%, serine 2.7%, glycine 2.3% histidine 1.8%, alanine 4.7%, cystine 1.2%, methionine 5.9%, leucine 4.8%, tyrosine 1.7%.
Embodiment 24
Get 1 kilogram of the chicken organ that cleans up, under 55 ℃ of conditions, add papain, stir, be incubated 4 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid (the alcohol layer Recycled ethanol is to most, and residue discards), under 35 ℃ of conditions, add trypsase, insulation 4h filters, and is concentrated, drying namely gets 0.508 kilogram in feed.
Feed contains: polypeptide weight content 35.0%, amino acid weight content 59.075%.Weight percentage is respectively aspartic acid 1.45% in the amino acid, glutamic acid 9.90%, threonine 2.65%, arginase 12 .5%, proline 3 .04%, valine 0.05%, isoleucine 2.46%, phenylalanine 2.98%, lysine 4.89%, serine 2.97%, glycine 2.67% histidine 1.75%, alanine 4.98%, cystine 1.35%, methionine 6.34%, leucine 5.23%, tyrosine 1.90%.
Embodiment 25
Get the chicken organ that cleans up, under 35 ℃ of conditions, add pepsin, stir, be incubated 3 hours, add the machine etoh solvent, mix, left standstill 2 hours, and separated clear liquid, filter, the strong-acid type cation exchange column washes with water on the filtrate, and eluent discards, be 15% ammoniacal liquor wash-out with concentration, collect eluent, concentrated, remove ammonia to most, drying, and get final product.
Feed contains: polypeptide weight content 30.2%, amino acid weight content 42.625%.Weight percentage is respectively aspartic acid 1.1% in the amino acid, glutamic acid 5.2%, threonine 1.2%, arginase 12 .2%, proline 2.1%, valine 0.025%, isoleucine 2.1%, phenylalanine 2.0%, lysine 4.1%, serine 2.2%, glycine 2.2% histidine 1.6%, alanine 4.1%, cystine 1.1%, methionine 5.6%, leucine 4.2%, tyrosine 1.6%.
Described embodiment includes but not limited to above-mentioned.
Claims (1)
1. the feed that chicken organ is raw material is characterized in that containing in the feed polypeptide weight content 35.5%, amino acid weight content 60.84%, aspartic acid 1.3%, glutamic acid 10.4%, threonine 2.9%, arginase 12 .4%, proline 3 .1%, valine 0.04%, isoleucine 3.0%, phenylalanine-3,4-quinone .2%, lysine 5.7%, serine 3.3%, glycine 2.6%, histidine 1.8%, alanine 5.7%, cystine 1.4%, methionine 6.8%, leucine 5.2%, tyrosine 2.0%; Its preparation method is: get 10 kilograms of the chicken organs that clean up, add pepsin under 40 ℃ of conditions, stir, be incubated 3 hours, add organic solvent ethanol, mix, left standstill 2 hours, and separated clear liquid, the alcohol layer Recycled ethanol is to most, residue discards, and filters, and filtrate is through molecular cut off 6000 milipore filter ultrafiltration, keep and see through liquid, concentrated, drying namely gets 6.43 kilograms in feed.
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| CN112841406A (en) * | 2021-01-25 | 2021-05-28 | 窦靖鑫 | Deep processing technology of black-bone chicken and black-bone chicken polypeptide protein powder |
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| US4579660A (en) * | 1983-06-14 | 1986-04-01 | Edinen Centar Po Chimia | Method for treatment of biomass |
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| CN101849622A (en) * | 2009-04-02 | 2010-10-06 | 凌文忠 | Preparation method of feed of hedgehog |
| CN102180945B (en) * | 2010-09-01 | 2012-11-07 | 中国科学院南海海洋研究所 | Small peptides extracted from internal organs of squids, method for preparing same, composition thereof and use thereof as protein source of marine aquatic feed |
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