JP4498593B2 - Hair restorer composition - Google Patents

Description

【００２５】
表１の結果から、本発明に係る育毛剤組成物は優れた育毛効果を有していることが分かる。また、比較例１と比較して、マウスの皮膚投与部位は、試験期間中を通じて影響はなく、実施例の試料による皮膚異常は認められなかった。
【００２６】
（試験例２：マウス毛包由来細胞の増殖に対する効果）
１．試料溶液の調製
実施例１の試料につき、マウス毛包由来細胞の増殖効果を試験した。まず、各試料を培養液に添加したときの最終濃度が後記表２及び３に示す濃度となるように、９９．９％エタノールに溶解した。また、比較例２としては、ミノキシジルを培養液に添加したときの最終濃度が７０μｇ／ｍｌとなるように、９９．９％エタノールに溶解したものを用いた。
【００２７】
▲２▼毛根の回収
生後４日齢のＣ３Ｈ／ＨｅＳｌｃ系新生仔マウスの皮膚を無菌的に採取し、１０％ＦＢＳ−ＤＭＥＭ培地で数回洗浄した。筋組織を取り除き、皮膚片を約１ｍｍ幅の短冊状に切り、毛包下部が現れるよう真皮結合組織を剥離した。出来るだけ多くの完全な毛球が得られるよう、メスで真皮組織をさらに細かく分け、０．２％コラゲナーゼＤＭＥＭ培養液（カルシウム、マグネシウム不含）で６０分間、３７℃でインキュベートした後、５℃に冷し、１０％ＦＢＳ−ＤＭＥＭ培地を加え反応を止め、毛球を回収した。
【００２８】
▲３▼細胞の培養
得られた毛根をトリプシン処理し、毛球部分の細胞（主に毛母細胞）を得、この細胞を１０％ＦＢＳ−ＤＭＥＭ培地に分散させコラーゲンコートした９６ウェルマイクロプレートに播種した。５％ＣＯ２，３７℃条件下で２４時間培養した後、培養液を試験培地（ＭＣＤＢ１５３培地に、５μｇ／ｍｌのインシュリン、５ｎｇ／ｍｌのＥＧＦ、０．５μｇ／ｍｌのヒドロコルチゾン、及び３５μｇ−protein ／ml−Bovine Pituitary Extractを添加したもの）に試験物質又は９９．９％エタノールのみを１／１００容添加したものに交換し、引き続き同じ条件で４日間培養した後、細胞数を測定した。また、対照例として、９９．９％エタノールのみを添加したものについても同様に培養し、細胞数を測定した。尚、比較例２及び対照例の試料を用いた試験は、上記と同じ条件下で行った。
【００２９】
３．細胞増殖
実施例１及び比較例２の試料溶液を添加した場合について得られた細胞数を、対照例について得られた細胞数と比較し、細胞増殖比の平均値±標準偏差を算出した。対照例との有意差検定は、危険率５％（ｐ＜０．０５）を有意とし、ｔ−検定を行った。
【００３０】
結果を表２に示す。
【表２】

【００３１】
表２の結果から、実施例１の試料は、比較例２のミノキシジルよりも低濃度で同等程度以上の優れた毛包細胞増殖活性を有していることが分かる。
【００３２】
【表３】
（処方例１）
育毛剤 配合量（重量％）
実施例で得られた抽出物 ５．０
酢酸トコフェロール ０．１
パントテニルアルコール ０．２
ニコチン酸アミド ０．１
ヒノキチオール ０．０５
ポリオキシエチレン（Ｅ０６０）
硬化ヒマシ油 ０．３
香料 ０．１
１，３−ブタンジオール ２．０
エタノール ５５．０
精製水 残 部
合計 １００．０
【００３４】
【発明の効果】
本発明は、有効成分として、アキャントスペルナムヒスピジウム（Acanthospermum hispidium）の抽出物が含有されてなることを特徴とする育毛剤組成物に関するものであるから、頭皮に対して好ましくない刺激を与えることなく、しかも、毛包細胞増殖活性作用を有し、毛包を活性化して優れた育毛効果を発揮することができる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a hair restorer composition, and an object thereof is to provide a highly safe hair restorer composition that has an excellent hair restoring effect and that does not give undesired stimulation to the scalp. .
[0002]
[Prior art]
Hair is made by cell division of hair matrix cells that are fed through capillaries distributed in the hair papilla. The hair does not continue to grow semipermanently. Normally, after growing for 2 to 5 years for males and 4 to 6 years for females (growth stage), keratinization of the hair root proceeds (transition period) Finally, the hair comes off (rest period). And hair is formed again after a certain period of time.
[0003]
As described above, since the hair continues to grow according to a certain cycle (hair cycle), in normal hair, 85% to 90% of the hair in the growth period and about 1% of the hair in the transition period are resting. The hair of the period is kept at a substantially constant rate of 14 to 19%. However, if this cycle breaks down for some reason, the proportion of hair in the growing phase and hair in the resting phase is disturbed, and consequently, symptoms of hair loss are exhibited.
General alopecia can be classified into resting hair alopecia, in which the resting hair is abnormally lost, and growing hair alopecia in which atrophy hair is observed, specifically, resting hair alopecia Examples of alopecia include androgenetic alopecia, alopecia due to dandruff, seborrheic alopecia and the like, and alopecia areata can be exemplified as the growth alopecia areata.
[0004]
[Problems to be solved by the invention]
The causes of alopecia include autoimmunity, peripheral nerve abnormalities, mental stress, bacterial infection of the scalp, etc., but the direct cause is considered to be nutritional deficiency of hair matrix cells. Therefore, hair loss can be prevented and hair growth can be promoted if the nutritional deficiency of hair matrix cells can be resolved and the function can be restored to normal.
Therefore, various active ingredients are blended in the current hair restorer. Specifically, the keratolytic agent for the epidermis to prevent the occurrence of dandruff in the scalp, and the fungicide for preventing the growth of bacteria in the scalp. , Blood flow promoters for improving blood circulation by promoting blood flow in capillaries connected to hair papilla, hair matrix cell activators for activating hair matrix metabolism and promoting hair growth, etc. Is blended.
However, the above-mentioned active ingredients of the conventional hair-restoring agents are not sufficiently satisfactory in the hair-restoring effect, and may give undesired stimulation to the scalp.
[0005]
[Means for Solving the Problems]
The present invention has been made to solve the above problems, and the invention according to claim 1 is characterized in that an extract of Acanthospermum hispidium is contained as an active ingredient. It is related with the hair restorer composition characterized.
[0006]
DETAILED DESCRIPTION OF THE INVENTION
The hair restorer composition according to the present invention is characterized by containing an extract of Acanthospermum hispidium as an active ingredient.
[0007]
Acanthospermum hispidium (Acanthospermum hispidium) is an annual plant of the Asteraceae family and is widely distributed in tropical regions of Africa and South America. In the Republic of Benin, Africa, it is used as a traditional medicine to treat infectious diseases. It is called “umbima” among the Shona people in the Republic of Zimbabwe, and is called “sibama yauli” among the Ndebele people.
[0008]
In the present invention, all parts of the above-ground part and underground part of Acanthosperm hispidium can be used, and each part such as a seed, a fruit part, a flower part, a leaf part, a root part, and a stem part is used. You may use individually or in mixture as appropriate. Moreover, the thing of a dry state and a non-dry state are used suitably. In the present invention, leaves and stems are preferably used.
[0011]
The above-mentioned dry pulverized material of the plant raw material can be used as it is as an active ingredient of the hair restorer composition, but a solvent extract is used . The solvent used in obtaining the solvent extract is not particularly limited, but is anhydrous or water-containing organic solvent, particularly monohydric alcohol, polyhydric alcohol or derivatives thereof, ketone, ester, ether, petroleum ether, aliphatic hydrocarbon or halogen. It is preferable to use an anhydrous or water-containing organic solvent containing one or more compounds selected from chemicals and aromatic hydrocarbons, since the hair-growth effect is sufficiently exhibited. Specifically, monohydric alcohols having 1 to 8 carbon atoms such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octanol, ethylene glycol , Propylene glycol, 1,3-butylene glycol, hexylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, or other polyhydric alcohols or derivatives thereof , Acetone, methylacetone, ethylmethylketone, isobutylmethylketone, methyl-n-propylketone, etc., C3-C6 ketone, ethyl acetate, isopropyl acetate, etc. C4-C8 ethers such as esters of number 4-5, ethyl ether, isopropyl ether, n-butyl ether, etc., petroleum ethers, petroleum ether, n-butane, n-pentane, n-hexane, n-octane, etc. 1 of 8 aliphatic hydrocarbons, halogenated aliphatic hydrocarbons having 1 to 2 carbon atoms such as carbon tetrachloride, chloroform, dichloroethane and trichloroethylene, and aliphatic hydrocarbons having 6 to 7 carbon atoms such as benzene and toluene. Preferred examples include anhydrous or hydrous organic solvents containing two or more species or species.
[0012]
Although the usage-amount of an above-described solvent is not specifically limited, It is 1-15 weight times with respect to the plant raw material with which extraction operation is provided, Preferably it is 3-10 weight times, More preferably, it is 3-7 weight times.
In addition, the extraction operation is not particularly limited, and the above-described plant raw material or a dry pulverized product thereof is extracted by immersing it at room temperature for 1 to 30 days, or reflux extraction or Soxhlet extraction at a temperature around the boiling point with the above-mentioned solvent. A method of extracting using a container or the like can be exemplified.
[0013]
An extract obtained by distilling off the solvent from the extract obtained from the above extraction solvent can be used. However, when ethanol is used as the extraction solvent, ethanol is highly safe for the skin, so It can be used without distilling off the solvent.
[0014]
Moreover, in this invention, the extract obtained by further refine | purifying the extract obtained by said method can be used as an active ingredient.
The purification treatment may be any method that is usually performed, and examples thereof include liquid-liquid distribution and liquid column chromatography.
In the case of a purification treatment by liquid column chromatography, examples of the packing material packed in the column include ion exchange resin, alumina, silica gel, agarose gel and the like. In addition, the purification process by liquid column chromatography may follow a conventional method.
[0015]
Hair restorer composition according to the present invention, active ingredient content, prepared by the method as described above is blended, the blending amount thereof is not particularly limited, since the effect of the active ingredient formulation is too small is not sufficiently exerted, the total composition The content is desirably 0.01 to 100% by weight, more preferably 0.1 to 20% by weight, and particularly preferably 1 to 5% by weight. It is also possible to only extract the plant with hair restorer composition.
[0016]
In addition to the above-mentioned active ingredients, the hair restorer composition according to the present invention includes, for example, vitamin E and its derivatives, assembly extract, tocopherol, ginseng extract, methyl nicotinate, benzyl nicotinate, Circulatory agents such as garlic extract, cephalanthin, carpronium chloride, Japanese cypress extract, acetylcholine, local stimulants such as pepper tincture, cantalis tincture, ginger tincture, nonyl acid vanillamide, keratin such as phenol, urea, salicylic acid, resorcin, lactic acid Solubilizer, placenta extract, pentadecanoic acid glyceride, pantothenyl ethyl ether, biotin, hinokitiol, linoleic acid, linolenic acid, arachidonic acid, succinic acid, citric acid, allantoin, photosensitizer 301 and other metabolic activators, glycyrrhizic acid Derivatives thereof, diphenhydrazine hydrochloride, hydrocortisone, chlorpheniramine aleate, glycyrrhetinic acid and other anti-inflammatory agents, orthomethoxycinnamaldehyde, methyl paraoxybenzoate, ethyl paraoxybenzoate, isopropylmethylphenol, sorbic acid, benzalkonium chloride, Fungicides such as isopropylmethylphenol, triclosan, zinc pyrithione and hinokitiol, refreshing agents such as menthol, mint oil, camphor, and other female hormones such as estriol, estrone, estradiol, ethinyl estradiol, etc. .
[0017]
Furthermore, within the range not impairing the effects of the present invention, monohydric alcohols such as ethanol, n-propanol, isopropanol, phenoxyethanol, polyhydric alcohols such as ethylene glycol, propylene glycol, butylene glycol, glycerin, diglycerin, dipropylene glycol, Water-soluble polymers, antioxidants, pH adjusters, UV inhibitors, sequestering agents, thickeners, surfactants, purified water, fragrances, preservatives, antibacterial agents, fats and oils, higher fatty acids, sterols Ordinary cosmetic ingredients such as fatty acid esters, moisturizers, refreshing agents, pigments, or hormones, vitamins, amino acids, astringents and placenta extracts, elastin, collagen, mucopolysaccharides, aloe extract, loofah water, royal jelly , Birch, carrot extract, chamomile extract, licorice extract, sa Biaekisu, Altea extract may be formulated into any appropriate according to the purpose of the special ingredients of animal and plant extract component or the like, including herbal components such as yarrow extract.
[0018]
In addition, the said hair restorer composition can be used as cosmetics, a quasi-drug, or a pharmaceutical, for example, can be used as a hair tonic, a hair cream, and a hair treatment.
[0019]
【Example】
EXAMPLES Hereinafter, although this invention is demonstrated in detail based on an Example, this invention is not limited to these Examples.
(Preparation of the sample of Example 1)
6 liters of ethanol was added to 2.6 kg of dry pulverized leaves and stems of Acanthospermum hispidium, and the mixture was reflux extracted at reflux temperature for 3 hours. The insoluble plant residue was filtered off, and 87 g (yield 3.4%) of the extract obtained by distilling off the filtrate under reduced pressure was used as the sample of Example 1.
[0021]
(Test Example 1: Effect on hair growth in mice)
1. The specimen of Example 1 of the sample solution and those dissolved in 99.9% ethanol to a concentration of from 5% (W / V) and the sample solution. Further, 99.9% ethanol was used as the sample solution of Comparative Example 1.
[0022]
2. Application to Mice C3H / HeN Crj mice (8 weeks old, body weight 21-26 g) were acclimated for more than one week, and those with no abnormalities were covered with an electric clipper on the back coat of 2 cm × 4 cm wide. The hair was shaved and further removed with an electric shaver to provide a sample solution administration site. After three days of hair removal, Example 1及 beauty Comparative Example 1 in the sample solution 19 consecutive days in each 10 mice and each 100 [mu] l, was applied in the morning once a day. During the test period, C3H / HeN Crj mice were placed in plastic cages (22 ± 2 ° C., relative humidity 55 ± 15%, ventilation rate 20 times / hour, in a breeding room set at 6 am to 6 pm irradiation time). 14.5 cm × 26 cm × 12.5 cm) were used for each 5 animals.
[0023]
The state of the specimen application site was regularly observed, scored according to the following evaluation criteria, and the average score of 10 animals was calculated.
Skin turns pink… 0 point skin changes to gray (less than 100%)… 1 point skin changes to gray (100%)… 2 points hair growth turns brown (less than 100%)… 3 points hair growth Changes to brown (100%) ... 4 point hair growth changes to black ... 5 point In addition, the skin administration site of the mouse during the test period is visually confirmed at the time of the evaluation, and whether skin abnormalities such as redness appear Observed at the same time.
[0024]
[Table 1]

[0025]
From the results in Table 1, it can be seen that the hair restorer composition according to the present invention has an excellent hair restoring effect. In comparison with Comparative Example 1, the skin site of administration mice, no effect throughout the study period, skin abnormalities by specimen examples were not observed.
[0026]
(Test Example 2: Effect on proliferation of mouse hair follicle-derived cells)
1. Preparation of sample solution The proliferation effect of mouse hair follicle-derived cells was tested on the sample of Example 1 . First, each sample was dissolved in 99.9% ethanol so that the final concentration when the sample was added to the culture solution was the concentration shown in Tables 2 and 3 below. Moreover, as Comparative Example 2, a solution dissolved in 99.9% ethanol was used so that the final concentration when minoxidil was added to the culture solution was 70 μg / ml.
[0027]
(2) Collection of hair roots Skin of 4 day old C3H / HeSlc newborn mice was aseptically collected and washed several times with 10% FBS-DMEM medium. The muscle tissue was removed, the skin piece was cut into a strip of about 1 mm width, and the dermis connective tissue was peeled off so that the lower part of the hair follicle appeared. In order to obtain as many complete hair bulbs as possible, the dermal tissue is further subdivided with a scalpel and incubated for 60 minutes at 37 ° C. in 0.2% collagenase DMEM medium (without calcium and magnesium), followed by 5 ° C. The reaction was stopped by adding 10% FBS-DMEM medium, and the hair bulb was collected.
[0028]
(3) Cell culture The hair root thus obtained was treated with trypsin to obtain hair bulb cells (mainly hair matrix cells), which were dispersed in a 10% FBS-DMEM medium and coated on a collagen-coated 96-well microplate. Sowing. After culturing for 24 hours under conditions of 5% CO 2 and 37 ° C., the culture solution was added to the test medium (MCDB153 medium, 5 μg / ml insulin, 5 ng / ml EGF, 0.5 μg / ml hydrocortisone, and 35 μg-protein / ml-Bovine Pituitary Extract) was added to 1/100 volume of the test substance or 99.9% ethanol alone, and subsequently cultured under the same conditions for 4 days, and then the number of cells was measured. In addition, as a control example, a sample to which only 99.9% ethanol was added was also cultured and the number of cells was measured. In addition, the test using the sample of the comparative example 2 and the control example was performed on the same conditions as the above.
[0029]
3. Number resulting cells to the case of adding a sample solution cell proliferation Example 1及 beauty Comparative Example 2, compared to the cell number obtained for control example, to calculate the mean ± standard deviation of cell growth ratio. The significant difference test with the control example was t-test with a significance level of 5% (p <0.05).
[0030]
The results are shown in Table 2 .
[Table 2]

[0031]
The results in Table 2, the specimen of Example 1, it is found to have the same degree or more superior hair follicle cell proliferation activity at lower concentrations than minoxidil Comparative Example 2.
[0032]
[Table 3 ]
(Prescription Example 1)
Hair restorer blending amount (% by weight)
Extract obtained in Example 5.0
Tocopherol acetate 0.1
Pantothenyl alcohol 0.2
Nicotinamide 0.1
Hinokitiol 0.05
Polyoxyethylene (E060)
Hardened castor oil 0.3
Fragrance 0.1
1,3-butanediol 2.0
Ethanol 55.0
Purified water balance
Total 100.0
[0034]
【The invention's effect】
The present invention relates to a hair restorer composition characterized by containing an extract of Acanthospermum hispidium as an active ingredient, and thus gives unpleasant irritation to the scalp. Moreover, it has a hair follicle cell proliferation activity and can activate the hair follicle to exhibit an excellent hair growth effect.

Claims (1)

A hair restorer composition comprising an extract of Acanthospermum hispidium as an active ingredient.