KR101881417B1 - Cosmetic composition comprising menthol, extracts of ginkgo biloba leaf, aloe barbadensis, camellia sinensis, centella asiatica and polygonum multiflorum - Google Patents

Abstract

The present invention menthol, Ginkgo (Ginkgo biloba Leaf extract, Aloe Barbadensis extract, Camellia Sinensis extract, Centella Asiatica Extract and Polygonum Multiflorum ) extract as an active ingredient. The cosmetic composition may exhibit hair growth promoting or hair loss prevention effect.

Description

COSMETIC COMPOSITION COMPRISING MENTHOL, EXTRACTS OF GINKGO BILOBA LEAF, ALOE BARBADENSIS, CAMELLIA SINENSIS, CENTELLA ASIATICA AND POLYGONUM MULTIFLORUM, comprising menthol, ginkgo leaf, aloe, green tea,

The present invention menthol, Ginkgo (Ginkgo biloba Leaf extract, Aloe Barbadensis extract, Camellia Sinensis extract, Centella Asiatica Extract and Polygonum Multiflorum ) extract as an active ingredient. The cosmetic composition may exhibit hair growth promoting or hair loss prevention effect.

The human hair has about 100,000 to 150,000 hairs, and each hair grows and falls through anagen, catagen, and telogen with different cycles. This cycle repeats over 3 to 6 years, with an average of 50 to 100 hairs per day dropping normally.

Although the mechanism of hair loss has not been elucidated yet, it is generally known to be caused by unbalanced dietary habits, excessive drinking and smoking, drug abuse, insufficient sleep, excessive stress, childbirth, menopausal disorders, frequent perms and staining, and hormonal imbalance . The hair growing in a person's hair grows and maintains a constant density by repeating the action of falling and growing. However, when the hair loss is progressed due to various reasons described above, hair on the parietal or forehead becomes tapered, The number is increased. In other words, people with baldness tend to have a general tendency to increase hair loss due to hereditary factors, acquired natural hair loss, various diseases, etc. Baldness people suffer a great deal of emotional stress, It is tilting.

Various methods for treating hair loss have been studied and proposed, but they are ineffective for long-term use. However, it is known that a method of hair transplantation which requires costly hair transplantation method can not be achieved completely.

In order to solve such a problem, it is required to develop a material which is more effective in promoting hair growth and preventing hair loss, and which can maintain safety against skin during long-term application.

Korean Patent No. 10-0681699

It is an object of the present invention to provide a method for producing menthol, ginkgo biloba Leaf extract, Aloe Barbadensis extract, Camellia Sinensis extract, Centella Asiatica Extract and Polygonum Multiflorum ) extract as an active ingredient.

In order to accomplish the above object, one aspect of the present invention is to provide a method for producing menthol, ginkgo biloba Leaf extract, Aloe Barbadensis extract, Camellia Sinensis extract, Centella Asiatica extract and Polygonum Multiflorum extract as an active ingredient. Specifically, the composition may further include one or more vitamin components selected from the group consisting of biotin, panthenol, tocopheryl acetate, and nicotinic acid amide.

In the present invention, " menthol " is known as the main component of mints. According to traditional oriental medicine, mint has a refreshing effect, a spasolysio effect, and it is known that it has a heat ray and a sea pound effect. It is also known to act on the blood vessel center and to relax blood vessels, thereby promoting blood circulation. It is known that this effect is due to menthol, which is the main component of mint, and it is known to exhibit circulatory effects through a strong incense and stimulation peculiar to menthol.

In the present invention, " Ginkgo biloba Leaf "is a leaf of Ginkgo biloba, which contains various flavonoids such as free radical removal of human body, antioxidant activity, protection of cell membranes, improvement of blood vessel disease, blood pressure lowering effect, various substances for blood circulation improvement, Is known to be contained. It is also known to have an anti-allergic effect as well as an antibacterial action.

In the present invention, " Aloe Barbadensis " is widely known as a plant that supplies moisture to the skin, and is known to form a moisture barrier film particularly because of excellent skin moisturizing effect. In addition, it exhibits an anti-inflammatory effect, exhibits excellent effects on wound healing, and is known to have an effect on cell regeneration. In the present invention, extraction was carried out using Jeju aloe.

In the present invention, " Camellia Sinensis " is an evergreen broad-leaved shrub belonging to the genus Chrysanthemum. Especially antioxidant activity, anticancer activity, blood lipid lowering effect in cardiovascular system, and blood circulation promoting action. In particular, Jeju green tea was used in the present invention.

In the present invention, " Centella Asiatica "is a perennial plant that is common in the mountains and fields of southern islands of Korea. The main stem extends sideways, and there are two degenerated scaly leaves near the node where the root descends. The main ingredients are beta-carotene, beta-sitosterol, campesterol, camphorol and saponin. Pharmacological actions include diuretic, tonic, nervous stability, anesthesia, immunostimulation, vomiting suppression, cholesterol suppression, It is known to have an effect. In the present invention, the extract was especially extracted using Jeju jujube.

In the present invention, " Polygonum Multiflorum ) "is a perennial herbaceous, vine medicinal plant belonging to the family Mallardiidae. It is used as a tonic, gangjeongje, tongkuk economy, hemicellulant, and chinhaeje, and it is known to be used for the treatment of cold, hematemesis, nervous breakdown and arthritis. In the present invention, extraction was carried out using Jeju sewage sludge.

In the present invention, biotin, panthenol, tocopheryl acetate and nicotinic amide are nutritional ingredients belonging to vitamins.

Biotin, also known as vitamin B7 or coenzyme R, is a water-soluble vitamin that is used as a component of hair, nails and skin nutrients.

Panthenol, also known as provitamin B5, is known to be effective in moisturizing and soothing skin, and is known to promote cell division and normalize keratinization of skin, wounds and anti-inflammatory effects.

Tocopheryl acetate is a type of vitamin E that exhibits antioxidant and moisturizing effects. When it comes in contact with vitamin C, it reproduces vitamin E particles consumed with vitamin C, thereby synergizing the skin and protecting the skin more effectively.

Nicotinic amide is a type of vitamin B group that helps energy production and is known to have effects on dermatitis and neuropathy along with nicotinic acid.

The "extract" of the present invention is an extract obtained by extracting the above mixture of Ginkgo biloba leaf extract, Aloe extract, Green tea extract, Centella asiatica, Centella asiatica extract and Sucrose extract as well as a mixture of the above extracts, Gingko leaf, Aloe, Green tea, Extracts of all the formulations which can be formed using the extract itself and extracts such as diluted or concentrated liquid of the extracted liquid, dried product obtained by drying the extracted liquid, prepared or purified product of the extracted liquid, or a mixture thereof.

The extract of the present invention can be extracted from natural, hybrid, or mutant plants of the respective plants, and can also be extracted from plant tissue cultures.

The method for extracting the extract of the present invention is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

The kind of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, anhydrous or lower alcohol of C ₁ to C ₄ , a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, chloroform Or a mixture of two or more of them.

More specifically, water (or distilled water) may be used as an extraction solvent in the present invention. The solvent extract may be prepared by extracting the ginkgo leaf, aloe, green tea, cinnabar, and / or tap water one or more times using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract can do. In a specific example of the present invention, a mixture of Ginkgo biloba extract, Aloe extract, Green tea extract, Centella asiatica and Centella asiatica extract and menthol was prepared using water (or distilled water) (Example 1) Panthenol, tocopheryl acetate and nicotinic acid amide (Example 2). The aloe, green tea, cinnabar, and seaweed were extracted using Jeju Aloe, Jeju Green Tea, Jeju Jujube, and Jeju Hwaseo.

In the present invention, the extract obtained by hot water extraction or cold extraction is filtered to remove suspended solid particles by filtration, for example, nylon or the like, or by freeze filtration, Spray drying and the like.

Specifically, the ginkgo leaf extract, the aloe extract, the green tea extract, the centilla sp. Extract and the extract of Sasa extract may be contained in a weight ratio of 1: 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10, May be contained in a proportion of 0.1 wt% to 3 wt% to prepare a mixture.

In one embodiment of the present invention, the mixture of ginkgo leaf extract, aloe extract, green tea extract, Centella asiatica and Centella asiatica extracts in a weight ratio of 5: 5: 5: 5: 5 and menthol content of 0.5 wt% These mixing ratios can be modified and used as needed. In particular, the raw materials of all the extracts of the present invention are derived from natural materials and thus have no toxicity. They are classified as EWG (Environmental Working Group) grade 1 and have been proved to have excellent safety. Therefore, even if a specific extract is contained in excess, .

Specifically, the cosmetic composition containing menthol, ginkgo leaf extract, aloe extract, green tea extract, centilla callus extract, and extract of Sasa extract as an active ingredient of the present invention may be used for promoting hair growth or preventing hair loss.

In the present invention, " hair growth " refers to hair in the growing stage in three stages of hair growth period, growth period, regenerator, and rest period. Promoting hair growth increases the proportion of hair during growth period, Quot; refers to the formation of individual hairs by differentiating into different cells and pushing out other cells out of the hair follicles.

The term " hair " refers to hair of the body and refers to a thread-like form composed of keratin proteins. Specifically, it may be hair, eyebrows, eyelashes, nose hair, or hair, but is not limited thereto.

In the present invention, " hair loss " refers to an increase in the number of abnormally dropped hair due to a shortening of hair growth rate in the growing period and an increase in hair in the retrograde period or in the resting period. Hair loss has been associated with the death and elimination of hair follicles, and male hormones have been reported to play a major role in the death and elimination of hair follicles.

In the present invention, " prevention of hair loss " is intended to prevent abnormal hair from falling off. It can be controlled by controlling the action of enzyme involved in testosterone, which is a male hormone, and activating dermal papilla cells.

In one embodiment of the present invention, when a mixture containing menthol, Ginkgo biloba extract, aloe extract, green tea extract, Centella asiatica extract, and Sasa extract was applied, excellent hair growth promoting effect (Table 1), 5-alpha reductase activity inhibitory effect (Table 2) and the activity of the dermal papilla cell (Table 3). Thus, it was confirmed that the effect of preventing and treating hair loss and the hair growth promoting effect can be shown. Further, it was confirmed that when the biotin, panthenol, tocopheryl acetate and nicotinic acid amide as vitamins were further mixed into the mixture, hair growth promoting effect, 5-alpha reductase activity inhibiting effect and dermal papilla cell activation effect were further enhanced .

In particular, the composition of the present invention may be characterized in that it is transdermally delivered through fine needle aspiration.

In the present invention, " fine needle " is thinner and finer fine needle than hair, which is used to physically create a small hole in the skin to create a drug penetration path. When such a fine needle is used, a drug having a large molecular weight or a drug having ionization or polarization can be effectively penetrated into the scalp.

The fine needle may be solid, hollow or soluble and is not limited thereto, and may be modified and used by using a suitable material if necessary. In addition, transdermal delivery may be performed through a method of contacting or attaching a patch or sheet having a plurality of microspheres to the skin, but the present invention is not limited thereto.

The hair follicles are more developed than other skin tissues and capillary vessels. The isthmus and the inferior portion of the skin around the skin nerve fibers (nerve fiber) tissue is most developed, and external stimuli and internal Respond quickly to stimuli. In addition, the thickness of the tissue is thinner than the skin tissue of the other region by about 1.5 mm. Especially, the scalp thickness of the region where the hair loss occurs is particularly sensitive because the hair is only 30 to 60% of the scalp tissue in which the hair exists.

On the other hand, in one embodiment of the present invention, it was confirmed that the composition of the present invention is suitable for transdermal delivery through micro needle without any pain, irritation, erythema, itching, and inflammation 5).

In addition, it was confirmed that when the composition of the present invention is delivered through fine needle-punching, the effect of the present invention can be not only confirmed from a faster time but also exhibits remarkably excellent hair growth promoting effect even after a certain period of time 6), the composition of the present invention may be transdermally delivered through fine needle aspiration, thereby exhibiting a more excellent effect.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening lotion, a pack, a soft water, a latex, a makeup base, But are not limited to, those selected from the group consisting of solutions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations, patches and sprays no.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the cosmetic composition, menthol, ginkgo biloba extract, aloe extract, green tea extract, Centella asiatica, and Sasa extract may be contained in an amount of 0.001% by weight to 30.0% by weight based on the total weight of the cosmetic composition. More specifically from 0.005% to 20% by weight. There is an advantage that excellent hair growth promotion or hair loss prevention effect is exhibited within the above range, and there is an advantage that the formulation of the composition is stabilized.

In addition, the composition of the present invention can be used as a transdermal administration method such as direct application or spraying on the skin, and the administration route of the composition of the present invention can be administered through any ordinary route as long as it can reach the target tissue.

The amount of the composition of the present invention to be used can be appropriately adjusted according to individual differences such as age and degree of lesion, and formulation, and can be applied to scalp once a day or several times a day for a week to several months.

The cosmetic composition containing menthol, ginkgo leaf extract, aloe extract, green tea extract, centilla sp. Extract, and extract of Sasa extract as an active ingredient of the present invention is excellent in hair growth promotion or hair loss prevention effect.

In addition, since the composition of the present invention is a substance derived from a natural substance, it has no toxicity and does not cause side effects even when it is applied to human body, and thus it is highly utilized as a cosmetic composition. Particularly, all the raw materials used in the present invention are classified as EWG (Environmental Working Group) grade 1, and thus it has been proved that the raw materials are excellent in safety.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example  One. menthol , Ginkgo leaf extract, aloe extract, green tea extract, Bedspread  The extract and Sewage  Extract Mixture 1  Produce

100 g of dry weight of each of ginkgo leaf, aloe, green tea, cinnabar, and seaweed was put into a flask and extracted with 1,000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare gingko leaf, aloe, green tea, cinnabar and cucumber extracts. The aloe, green tea, cinnabar, and seaweed were extracted using Jeju Aloe, Jeju Green Tea, Jeju Jujube, and Jeju Hwaseo. Each of the extracts was mixed in a weight ratio of 5: 5: 5: 5: 5, and menthol was mixed so as to contain 0.5 wt% of the total mixture.

Example  2. Example  1 < / RTI > Mixture 2  Produce

To the mixture of Example 1, biotin (vitamin B), panthenol (converted to vitamin B5), tocopheryl acetate (vitamin E) and nicotinic acid amide (vitamin B), which are vitamins, Respectively.

Comparative Example  1 to 5. Ginkgo biloba extract, aloe extract, green tea extract, Bedspread  Extract, Sucrose Extract, and Mixture of Each Extract and Menthol

The extracts of gingko leaf, aloe, green tea, cinnabar and sea bass were prepared in the same manner as in Example 1, and L-menthol was added to each extract so as to contain 0.5 wt% of the total mixture. The aloe, green tea, cinnabar, and seaweed were extracted using Jeju Aloe, Jeju Green Tea, Jeju Jujube, and Jeju Hwaseo.

As shown in Table 1 below, the respective extracts and mixtures of menthol were summarized as comparative examples.

Comparative Example 1 Ginkgo biloba extract + menthol Comparative Example 2 Aloe extract + menthol Comparative Example 3 Green tea extract + menthol Comparative Example 4 Centella asiatica extract + menthol Comparative Example 5 Sucrose Extract + Menthol

Experimental Example  1. Identification of hair growth promoting effect

A C57BL / 6 mouse model was used as an animal test model used for evaluation of hair growth promotion. C57BL / 6 mice are characterized by black hair, spontaneous alopecia, and melanocytes are present only in hair follicles, and melanin synthesis is responsible for the hair growth cycle. And it is widely used for evaluation of hair growth promotion because it has an advantage of being able to determine the growth period of hair with skin color.

The hair growth promoting evaluation test was carried out by using a mouse (C57BL / 6, male) of 42-56 days old, removing the hairs on the back region and cultivating 7 dogs of Example 1 and Comparative Examples 1 to 5, One hundred microliters was applied for 28 days. As a negative control, 30% ethanol was applied. The ratio of the area of newborn mothers to the area of hair removed was analyzed.

sample Area ratio of newborn mature area / hair removed area (%) After 9 days After 20 days Negative control group 4.11 54.93 Example 1 14.87 94.14 Example 2 15.55 95.86 Comparative Example 1 9.76 82.41 Comparative Example 2 8.80 79.88 Comparative Example 3 9.54 78.15 Comparative Example 4 8.11 74.36 Comparative Example 5 9.36 77.56

As shown in Table 2, even in the case of Comparative Example 1 to Comparative Example 5, hair growth effect was shown as compared with the negative control. However, compared with Comparative Examples 1 to 5, the hair growth promoting effect .

Experimental Example  2. 5- Alpha-reductase  Identification of the activity inhibition effect

The 5 alpha-reductase enzyme used in the inhibition of 5-alpha reductase activity was obtained from rat liver (adult, Sprague-Dawley, male rat (8-10 weeks old, testis) (protein suspension).

Radioactively labeled testosterone ( ³ H testosterone) was used as the substrate for the 5-alpha reductase enzyme (enzyme reaction solution: protein suspension: each mixture of Example 1 and Comparative Examples 1 to 5 = 70:20:10) for 30 minutes in an incubator, extracted with ethyl acetate, and developed with 100 microliters of ethyl acetate on a thin layer chromatograph (TLC). The conversion rate to developed testosterone was measured and the 5-alpha reductase inhibition rate was evaluated. The method of calculating the inhibition rate is as follows.

Conversion rate = amount of radioactivity in DHT area / total amount of radioactivity

5-alpha reductase activity inhibition rate (%) = [(A-B) / A] X 100

A = conversion of testosterone to dihydrotestosterone when no extract was added (negative control)

B = conversion of testosterone to dihydrotestosterone upon addition of extract

Dihydrotestosterone
(dpm) Testosterone
(dpm) Conversion Rate
(%) Inhibition rate
(%) Negative control group 18387 21845 45.7 - Example 1 3356 37625 8.1 82.2 Example 2 3289 39151 7.7 83.2 Comparative Example 1 8459 30056 21.9 52.0 Comparative Example 2 7486 31045 19.4 57.4 Comparative Example 3 7753 30597 20.2 55.7 Comparative Example 4 6986 31133 18.3 59.8 Comparative Example 5 7301 30338 19.4 57.5

As shown in Table 3, it was evaluated that the activity of 5-alpha reductase was significantly inhibited compared with those of Comparative Examples 1 to 5 when Examples 1 and 2 were applied. In particular, it was found that the highest inhibitory effect was observed in Example 2.

Experimental Example  3. Dermal papilla cells  Check activity effect

From the plastic surgeon, the human scalp tissue after the scalp surgery was obtained and the hair follicle tissue was separated therefrom. Specifically, the scalp tissue was immersed in 70% ethanol for one minute and sterilized and washed three times with M199, a cell culture medium. Under the stereoscopic microscope, the epidermis and the dermis were cut at the interface between the epidermis and the dermis by a surgical scalpel, and the hair follicles embedded in the dermis were extracted one by one using tweezers. The isolated hair follicles were transferred to M199 medium containing 10% fetal bovine serum (FBS) and the lower part of the hair follicle containing the papilloma was cut with a scalpel. The dermal papilla was separated using fine tweezers and transferred to M199 medium containing 20% FBS and cultured for 2 weeks. When the cells started to grow from the papilla tissue, when the culture container became full, the cells were separated from the culture container with 0.25% trypsin and 0.02% EDTA, and the cells were continuously cultured by subculture. Then, 2 to 3 passages of dermal papilla cells were diluted with M199 medium containing 10% FBS to a concentration of 2X10 ⁴ / ml, and then 5 ml was added to a 60 mm culture dish and cultured in a 37 ° C incubator for 24 hours. On the next day, the culture medium containing FBS was removed, and the culture medium containing no FBS (control) and the mixture of Example 1 and Comparative Examples 1 to 5 was exchanged and cultured for 24 hours. Then, Thymidine was added at 10 μCi per culture dish and further cultured for 24 hours. The cultured cells were washed three times with phosphate buffered saline and washed once with 5% trichloroacet-tic acid. Cells were dissolved in 0.1% sodium dodecylsulfonate and 0.5 N sodium hydroxide solution, mixed in a scintillation cocktail, and the amount of radioactive isotopes was measured using a beta-counter. The activity of the dermal papilla cells was compared with that of the control group by radioactivity labeled thymidine incorporation into the cells (uptake).

sample Dermal papillary cell activity (%) Control group 100 Example 1 161 Example 2 177 Comparative Example 1 115 Comparative Example 2 126 Comparative Example 3 138 Comparative Example 4 130 Comparative Example 5 128

As shown in Table 4, when the Example 1 and the Example 2 were applied, the activity of the dermal papilla cell was significantly higher than that of Comparative Examples 1 to 5, The highest activity was obtained. From the results, it was found that the composition of the present invention promotes the activity of the dermal papilla cells, thereby ultimately exhibiting preventive and therapeutic effects on hair loss and promoting hair growth.

Experimental Example  4. On fine needle  by Transdermal  Check conformity

(Scleroderma, hair density, pore status, hair thickness, hair follicle condition, hair condition, etc.) using a scalpel diagnosis apparatus (ARAMO-SG, (0.075 mm or more), even if the hair density is less than 70% of the normal hair density (the number of hair per 1 cm 2 area) (120 or more / 1 cm 2) or the hair density is close to normal, Ten patients were selected for the case of more hair loss (less than 0.5mm) and hair loss was ongoing.

Thereafter, the ampoule mixed with the extract of Example 2 was applied twice inside the arm of the subject according to the following Table 5, and then infiltrated using a micro-needle.

Raw material name Content (% by weight) ethanol 10 Propylene glycol 7 1,3-butylene glycol 5 Polysorbate 80 3 Salicylic acid 0.3 Nicotinamide 0.5 Dipotassium glycyrrhizate 0.5 Allantoin 0.5 Panthenol 0.5 glycerin 3 Bis-phage-18 methylethyldimethylsilane One antiseptic Suitable amount Example 2 [ 10 Purified water To 100

Erythema, irritation, itching, and inflammation were confirmed as the determinants of microsomal compatibility. (0: no erythema; 1: erythema but insufficient; 2: erythema but overall good; 3: erythema is severe). 4: erythema was very severe), and the extent of onset or persistence of itching after microneedle procedure was 0 to 4 (0: no itching, 1: itching but not enough, 2: itching, but overall good) (0: no inflammation, 1: inflammation but insufficient), and the degree of inflammation after the micro needle procedure was visualized through visual observation. 2: inflammation but overall good 3: severe inflammation 4: very inflammatory).

Symptom Degree Pain or irritation 0.2 Erythema 0.3 Itching 0.1 Inflammation 0.1

As shown in Table 6, it was confirmed that the composition of the present invention is suitable for transdermal delivery through microspheres.

Experimental Example  5. On fine needle  Confirmation of transfer effect by

5-1. Check hair growth promoting effect

The hair growth promoting effect was confirmed in the same manner as in Experimental Example 1, except that the mixture of Example 2 was simply applied to the skin and the case of microdermabrasion was compared.

Example 2 Area ratio of newborn mature area / hair removed area (%) After 9 days After 20 days Simple application 15.55 95.86 Fine needle 23.58 98.89

As shown in Table 7, when the mixture of Example 2 was transferred through microspheres, the hair growth promoting effect was more excellent. Particularly, in Example 2, it was confirmed through Experimental Example 1 that the hair growth promoting effect was excellent even in the composition itself, but when the hair was transferred through the micro needle, the effect was observed from the 9th day, which is a faster time.

Therefore, the composition of the present invention exhibits excellent effects even when it is applied in a simple manner. However, when the composition is delivered through a fine needle, the effect of the composition is not only confirmed from a faster time but also remarkably excellent after a certain period of time there was.

Manufacturing example 1. Bonn  Containing an extract of the invention hair ample  Produce

A cosmetic composition of ampoule formulation was prepared as shown in Table 8 below (unit: wt%).

Raw material name Content (% by weight) ethanol 10 Propylene glycol 7 1,3-butylene glycol 5 Polysorbate 80 3 Salicylic acid 0.3 Nicotinamide 0.5 Dipotassium glycyrrhizate 0.5 Allantoin 0.5 Panthenol 0.5 glycerin 3 Bis-phage-18 methylethyldimethylsilane One antiseptic Suitable amount The composition of the present invention 10 Purified water To 100

Manufacturing example 2. Bonn  Manufacture of hair cream containing the extract of the invention

A cream form cosmetic composition was prepared as shown in Table 9 below (unit: wt%).

Composition Content (% by weight) The composition of the present invention 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Suitable amount incense Suitable amount Purified water To 100

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

A cosmetic for enhancing hair growth and preventing hair loss comprising an extract of menthol, Ginkgo biloba Leaf extract, Aloe Barbadensis extract, Camellia Sinensis extract, Centella Asiatica extract and Polygonum Multiflorum extract as active ingredients Composition. The method according to claim 1,
Wherein the composition further comprises at least one vitamin component selected from the group consisting of biotin, panthenol, tocopheryl acetate and nicotinic acid amide. The method according to claim 1,
Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol of C ₁ to C ₄ , a mixed solvent of water and a lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate and chloroform And extracting the cosmetic composition. The method according to claim 1,
Menthol, ginkgo biloba extract, aloe extract, green tea extract, centilla sp. Extract and Sasa extract are contained in an amount of 0.001 wt% to 30.0 wt% with respect to the total weight of the cosmetic composition. The method according to claim 1,
Wherein the composition is transdermally delivered through a microspheres. 6. The cosmetic composition according to claim 5, wherein the microspheres are solid, hollow or soluble. 6. The method of claim 5,
Wherein the transdermal delivery is carried out by contacting or attaching a patch or sheet with a plurality of microspheres attached thereto. delete