JP2002167310A - Composition of hair restoration agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition of a hair restoration agent that dose not have unfavorably irritate the scalp as well as has an excellent hair restoration effect. SOLUTION: The hair restoration agent is characterized by containing Acanthospermum hispidium and/or Ammi visnaga (L.) Lam. as active ingredients.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hair restorer composition, and an object of the present invention is to provide a hair restorer composition which has an excellent hair restorer effect and does not give an unfavorable irritation to the scalp. To provide things.

[0002]

2. Description of the Related Art Hair is formed by cell division of hair matrix cells fed through capillaries distributed in the dermal papilla. Hair does not keep growing semi-permanently, usually 2-5 years for men and 4 for women
After growing for up to 6 years (growth period), the keratinization of the hair root progresses (transition period), and finally the hair comes off (quiescent period). Then, after a certain period of time, the hair is formed again.

[0003] As described above, the hair continues to grow according to a certain cycle (hair cycle), so that in normal hair, the hair in the growing phase is 85 to 90% of the total and the hair in the transition phase is 1%. About 14-19% of hair at rest period
It is kept at a nearly constant rate. However, if this cycle is disrupted for any reason, the proportion of anagen hair to telogen hair will be disturbed, resulting in the appearance of hair loss. Common alopecia can be classified into telogen baldness, in which telogen hair is unusually shed, and anagen baldness, in which atrophy is observed. As alopecia, androgenetic alopecia, alopecia due to dandruff, seborrheic alopecia, etc.
Alopecia areata can be exemplified.

[0004]

The causes of alopecia include autoimmunity, peripheral nerve abnormalities, mental stress, bacterial infection of the scalp, and the like.
It is thought that hair matrix cells are undernourished. Therefore, it is said that hair loss can be prevented and hair growth can be promoted if the function can be restored to normal by eliminating the nutritional deficiency of hair matrix cells. Therefore, various active ingredients are blended in the current hair restorer, specifically, a keratolytic agent for the epidermis to prevent the occurrence of dandruff on the scalp, and a bactericide to prevent the growth of bacteria on the scalp. A blood flow enhancer to promote blood flow in the capillaries connected to the dermal papilla to improve blood circulation, an activator of hair matrix to activate the metabolism of hair matrix and promote hair growth, etc. Is blended. However, the above-mentioned active ingredients of the conventional hair restorer are not sufficiently satisfactory in the hair restorer effect and sometimes give an unpleasant stimulus to the scalp.

[0005]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and the invention according to claim 1 is characterized in that Acanthospermum hispidium and Acanthospermum hispidium are used as active ingredients. The present invention relates to a hair restorer composition characterized by containing Ammi visnaga (L.) Lam. The invention according to claim 2 provides the present invention as an active ingredient, in the Republic of Zimbabwe, umbima or sibama yauli.
Plants and / or ammi-naga (Ammi)
visnaga (L.) Lam.) is contained.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The hair restorer composition according to the present invention comprises, as an active ingredient, acanthospernamipisdium (Ac)
anthospermum hispidium) and / or Ammi visnaga (L.) Lam.

[0007] Acanth spernum hispidium (Acan
thospermum hispidium) is an annual of Asteraceae and is widely distributed in tropical areas of Africa and South America. In the Republic of Benin in Africa, it is used as a traditional medicine for treating infectious diseases. It is called umbima among the Shona tribes of the Republic of Zimbabwe, and Ndebele
Among the ele) tribes, they are called shibama yauli.

In the present invention, all of the above and underground parts of Acanthospermum hispidium can be used, such as seeds, fruits, flowers, leaves, roots, stems and the like. Each part may be used alone or in combination as appropriate. Further, both those in a dry state and those in a non-dry state are suitably used. In the present invention, leaves and stems are preferably used.

[0009] Ammi visnaga (L.) La
m.) is a dicotyledonous plant belonging to the genus Apiaceae. Distributed around the Mediterranean coast, with a height of about 20-9
It is a perennial or annual plant reaching 0 cm. The flower pattern of the inflorescence is used for toothpicks, and seeds are used as a specific medicine for angina pectoris and bronchial asthma in Africa, the Middle East, Middle East Asia and the like.

[0010] In the present invention, Ammi naga (Ammi
visnaga (L.) Lam.) can be used in all parts, above and below the ground, and their parts such as seeds, fruits, flowers, leaves, roots, stems, etc. can be used alone or by mixing them appropriately. May be used. Further, both those in a dry state and those in a non-dry state are suitably used. In the present invention, it is preferable to use seed.

Although the above-mentioned dried and crushed plant material can be used as an active ingredient of the hair restorer composition as it is, it is preferable to use a solvent extract. The solvent used for obtaining the solvent extract is not particularly limited, but anhydrous,
Alternatively, a water-containing organic solvent, particularly an anhydride containing one or more selected from monohydric alcohols, polyhydric alcohols or derivatives thereof, ketones, esters, ethers, petroleum ethers, aliphatic hydrocarbons or halides, and aromatic hydrocarbons, Alternatively, it is preferable to use a water-containing organic solvent because the hair growth effect is sufficiently exhibited. Specifically, methanol, ethanol, isopropyl alcohol, n-propyl alcohol, isobutanol, n-hexanol, methyl amyl alcohol,
Carbon number 1 such as 2-ethylbutanol, n-octanol, etc.
~ 8 monohydric alcohol, carbon number 2 such as ethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, etc. To 6 polyhydric alcohols or derivatives thereof, acetone, methyl acetone, ethyl methyl ketone, isobutyl methyl ketone, methyl-n
C3 to C6 ketones such as propyl ketone, C4 to C5 esters such as ethyl acetate and isopropyl acetate, C4 to C8 ethers such as ethyl ether, isopropyl ether and n-butyl ether, and petroleum ethers; n-
C4 to C8 aliphatic hydrocarbons such as butane, n-pentane, n-hexane, and n-octane; and halides of C1 to C2 aliphatic hydrocarbons such as carbon tetrachloride, chloroform, dichloroethane, and trichloroethylene. ,benzene,
A preferable example is an anhydrous or water-containing organic solvent containing one or more kinds of aliphatic hydrocarbons having 6 to 7 carbon atoms such as toluene.

The amount of the solvent used is not particularly limited, but is 1 to 15 times, preferably 3 to 10 times, more preferably 3 to 10 times the weight of the plant material to be subjected to the extraction operation.
7 weight times. The extraction operation is not particularly limited,
Extraction method by immersing the above-mentioned plant material or its dried and crushed product at room temperature for 1 to 30 days, or extraction by reflux extraction or Soxhlet extractor with the above-mentioned solvent at a temperature around its boiling point, etc. Can be exemplified.

[0013] An extract obtained by distilling the solvent from the extract obtained by the above-mentioned extraction solvent can be used.
When ethanol is used as the extraction solvent, ethanol can be used without distilling off the solvent from the extract, because ethanol has high safety to the skin.

In the present invention, an extract obtained by further purifying the extract obtained by the above method can be used as an active ingredient. As this purification treatment, any method may be used as long as it is a usual method.
For example, liquid-liquid distribution and liquid column chromatography can be exemplified. In the case of the purification treatment by liquid column chromatography, examples of the packing material to be packed in the column include ion exchange resin, alumina, silica gel, agarose gel and the like. The purification by liquid column chromatography may be performed according to a conventional method.

The hair restorer composition according to the present invention is blended with one or two kinds of active ingredients prepared by the above-mentioned method, and the amount of the active ingredient is not particularly limited. Is not sufficiently exhibited, the content is desirably 0.01 to 100% by weight based on the total composition.
1-20% by weight is more preferable, and 1-5% by weight is particularly preferable. It is also possible to use only a plant dry powder or a plant extract as a hair restorer composition.

In the hair restorer composition according to the present invention, besides the above-mentioned active ingredients, as a hair restorer / hair restorer, for example, vitamin E and its derivatives, assembly extract, tocopherol, ginseng extract, methyl nicotinate, nicotine Blood circulation promoters such as benzyl acid, garlic extract, cepharanthin, carpronium chloride, carrot extract, acetylcholine, etc., local stimulants such as pepper tincture, cantaristin tincture, ginger tincture, nonylate vanillamide, phenol, urea, salicylic acid, resorcinol, lactic acid Keratolytic agents such as placenta extract, pentadecanoic acid glyceride, pantothenyl ethyl ether, biotin, hinokitiol, linoleic acid, linoleic acid, arachidonic acid, succinic acid, citric acid, allantoin, photosensitizer 301 and other metabolic activators; Anti-inflammatory agents such as luritic acid and its derivatives, diphenhydrazine hydrochloride, hydrocortisone, chlorpheniramine array acid, glycyrrhetinic acid, orthomethoxycinnamaldehyde, methyl parahydroxybenzoate, ethyl paraoxybenzoate, isopropylmethylphenol, sorbic acid, benzal chloride Fungicides such as ruconium, isopropylmethylphenol, triclosan, zinc pyrithione, hinokitiol, menthol, peppermint oil, refreshing agents such as camphor, and others, and female hormones such as estriol, estrone, estradiol, ethinyl estradiol, and the like may also be appropriately compounded. It is possible.

Furthermore, within a range that does not impair the effects of the present invention,
Ethanol, n-propanol, isopropanol, monohydric alcohols such as phenoxyethanol, ethylene glycol, propylene glycol, butylene glycol,
Polyhydric alcohols such as glycerin, diglycerin, dipropylene glycol, water-soluble polymers, antioxidants, pH adjusters, UV inhibitors, sequestering agents, thickeners, surfactants, purified water, fragrances, preservatives Agents, antibacterial agents, fats and waxes, higher fatty acids, sterols, fatty acid esters, moisturizers, fresheners, pigments and other usual cosmetic ingredients, or hormones, vitamins, amino acids, astringents and placenta extracts, elastin Special combination of animal and plant extracts, including crude drug components such as, collagen, mucopolysaccharide, aloe extract, loofah water, royal jelly, birch, carrot extract, chamomile extract, licorice extract, salvia extract, altea extract, and yarrow extract The components may be arbitrarily mixed according to the purpose.

The hair restorer composition can be used as cosmetics, quasi-drugs or pharmaceuticals, and can be used, for example, as a hair tonic, a hair cream or a hair treatment.

[0019]

EXAMPLES Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to these examples. (Preparation of sample of Example 1) 6 liters of ethanol was added to 2.6 kg of dried and crushed leaves and stems of Acanthospermum hispidium, and the mixture was reflux-extracted at reflux temperature for 3 hours. The extract 87 obtained by filtering off the insoluble plant residue and distilling off the filtrate under reduced pressure.
g (yield 3.4%) was used as the sample of Example 1.

(Preparation of Sample of Example 2) Dry and ground 2.0 seeds of Ammi visnaga (L.) Lam.
4 liters of ethanol was added to the kg, and the mixture was refluxed at reflux temperature for 3 hours. The insoluble plant residue was filtered off, and the filtrate was distilled off under reduced pressure to obtain 80 g of an extract (4.0% yield).
%) Was taken as the sample of Example 1.

(Test Example 1: Effect on Hair Growth in Mice) Preparation of Sample Solution Each sample of Examples 1 and 2 was prepared at a concentration of 5% (W / V).
Was dissolved in 99.9% ethanol to obtain a sample solution. Also, 99.9% ethanol was added to Comparative Example 1
Sample solution.

Application to mice C3H / HeN Crj mice (8 weeks old, body weight 21 to 2)
6g) was acclimated for at least one week, and for those without abnormality, the back hair was covered with an electric hair clipper for 2 cm.
The hair was shaved to a size of × 4 cm, and the hair was further removed using an electric shaver to obtain a sample solution administration site. Three days after the hair removal, the sample solutions of Examples 1 and 2 and Comparative Example 1 were applied to 10 mice each for 19 consecutive days in an amount of 100 μl once a day in the morning. During the test period, C3H / HeN Crj mice were placed in a plastic cage (22 ± 2 ° C., 55 ± 15% relative humidity, 20 ventilations / hour, irradiation time set from 6:00 am to 6:00 pm) in a breeding room. 14.5cm
X 26 cm x 12.5 cm).

The state of the sample application site was periodically observed, scored according to the following evaluation criteria, and the average score of 10 animals was calculated. Skin turns pink ... 0 point Skin changes to gray (less than 100%) ... 1 point Skin changes to gray (100%) ... 2 points Hair changes to brown (less than 100%) ... 3 points Hair growth Changed to brown (100%) ... 4 points Hair growth changed to black ... 5 points In addition, the skin administration site of the mouse during the test period was visually checked at the time of the evaluation, and it was determined whether or not skin abnormalities such as redness appeared. Observed at the same time.

The results are shown in Table 1.

[Table 1]

From the results shown in Table 1, it can be seen that the hair restorer composition according to the present invention has an excellent hair restorer effect. Also,
Compared with Comparative Example 1, the skin administration site of the mouse had no effect throughout the test period, and no skin abnormality was observed in each sample of the example.

(Test Example 2: Effect on Proliferation of Mouse Hair Follicle-Derived Cells) Preparation of Sample Solutions The proliferation effects of mouse hair follicle-derived cells were tested on each of the samples of Examples 1 and 2. First, each sample was dissolved in 99.9% ethanol so that the final concentration when added to the culture solution was as shown in Tables 2 and 3 below. In Comparative Example 2, 99.9% of minoxidil was added to the culture solution so that the final concentration was 70 μg / ml.
What was dissolved in ethanol was used.

Collection of Hair Roots The skin of newborn C3H / HeSlc mice 4 days after birth was aseptically collected and washed several times with 10% FBS-DMEM medium. The muscle tissue was removed, the skin piece was cut into a strip having a width of about 1 mm, and the dermal connective tissue was peeled off so that the lower part of the hair follicle appeared. In order to obtain as many complete hair bulbs as possible, the dermal tissue is further subdivided with a scalpel and incubated at 37 ° C. for 60 minutes in a 0.2% collagenase DMEM culture medium (containing no calcium and magnesium), and then at 5 ° C. The reaction was stopped by adding 10% FBS-DMEM medium, and the hair bulb was collected.

Culture of cells The obtained hair root is treated with trypsin to obtain cells in the hair bulb portion (mainly hair mother cells), and these cells are cultivated in 10% FBS-DMEM.
The cells were dispersed in a medium and seeded on a 96-well microplate coated with collagen. 5% CO2, 2 at 37 ° C
After culturing for 4 hours, the culture solution was added to the test medium (MCDB153).
The medium contains 5 μg / ml insulin, 5 ng / ml EGF, 0.5 μg / ml hydrocortisone, and 3 μg / ml hydrocortisone.
5 μg-protein / ml-Bovine Pituitary Extract) was added to the test substance or 99.9% ethanol alone in 1/100 volume. After culturing for 4 days under the same conditions, the number of cells was measured. did. In addition, as a control, a cell to which only 99.9% ethanol was added was similarly cultured, and the number of cells was measured. In addition, the test using the samples of Comparative Example 2 and Control Example was performed under the same conditions as described above.

Cell Proliferation The number of cells obtained when the sample solutions of Examples 1 and 2 and Comparative Example 2 were added was compared with the number of cells obtained for the control, and the average value of cell proliferation ratio ± standard deviation Was calculated. The significant difference test with the control was performed with a risk ratio of 5% (p <0.0
5) was considered significant, and a t-test was performed.

The results are shown in Tables 2 and 3.

[Table 2]

[Table 3]

From the results shown in Tables 2 and 3, it can be seen that the samples of Examples 1 and 2 have excellent hair follicle cell proliferation activity at a lower concentration than that of Minoxidil of Comparative Example 2 and at least equivalently. .

Hereinafter, formulation examples of the hair restorer composition according to the present invention will be described.

[Table 4]

[0033]

[Table 5]

[0034]

According to the present invention, as an active ingredient, Acanthospermum hispidium is used.
And / or Ammi visnaga (L.) Lam.
Since it relates to a hair restorer composition characterized by comprising, without giving an unpleasant stimulus to the scalp, and has a hair follicle cell proliferation activity,
It can activate hair follicles and exert an excellent hair growth effect.

Continued on the front page (72) Inventor Toshihiro Sakano 5-12, Junkencho, Chuo-ku, Osaka City Inside Mandom Central Research Institute Co., Ltd. (72) Inventor Satoshi Hashigaki 5-12, 12, Junkencho, Chuo-ku, Osaka-shi Mandom Central Research Institute Co., Ltd. (72) Inventor Jean-Maurice Mann Grasse, France 06130 Le Tude Saint Matthew, 290 Saint Matthew Domain F-term (reference) 4C083 AA111 AC102 AC122 AC432 AC642 AC852 AD662 CC37 DD23 EE22 FF01 4C088 AB26 AB40 AC03 AC04 AC05 AC11 BA08 CA03 CA14 MA63 NA14 ZA92

Claims (2)

[Claims] 1. A hair restorer composition comprising, as an active ingredient, Acanthospermum hispidium and / or Ammi visnaga (L.) Lam. 2. The active ingredient in the Republic of Zimbabwe is umbima or sibama yau
li) A hair restorer composition comprising a plant and / or Ammi visnaga (L.) Lam.