JP4355560B2 - 有害物質のモニタリング方法 - Google Patents
有害物質のモニタリング方法 Download PDFInfo
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- JP4355560B2 JP4355560B2 JP2003391645A JP2003391645A JP4355560B2 JP 4355560 B2 JP4355560 B2 JP 4355560B2 JP 2003391645 A JP2003391645 A JP 2003391645A JP 2003391645 A JP2003391645 A JP 2003391645A JP 4355560 B2 JP4355560 B2 JP 4355560B2
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Description
以下の(I)式で定義される酸素消費率を所定の範囲内に維持するように、前記測定溶液中の前記基質の濃度を制御することを特徴とする。
(上記(I)式において、V1は、有害物質及び基質を含まない緩衝溶液と純水とを流した際の微生物センサの電気的出力、V2は測定溶液を流した際の微生物センサの電気的出力を表す。)
本発明の有害物質のモニタリング方法によれば、上記の酸素消費率を指標として基質濃度を制御することによって、試料溶液の栄養状態が異なる様々な水質においても、固定化膜の微生物の活性度を常に最適な感度状態に調整できる。すなわち、低過ぎる酸素消費率(固定化膜の微生物の活性度低下)によって生じる、溶存酸素電極のノイズ等の外乱による誤警報の発生や、高過ぎる酸素消費率(固定化膜の微生物の活性度が過剰に高い場合)によって生じる感度低下や感度のバラツキを防止できる。その結果、様々な水質の試料溶液に対しても有害物質に対する検出感度を低下させることなく、長期にわたって安定して有害物質をモニタリングすることができる。また、基質濃度を変動させて、微生物の基質酸化反応速度を化学的に調整することから、温度制御に比べて短時間で活性度の調整が可能である。
すなわち、酸素消費率は、固定化微生物膜1に担持された微生物の活性度を示し、微生物の呼吸量の最大値に対する比率である。そして、本発明においては、この酸素消費率を所定の範囲内に維持するように、測定溶液中の基質の濃度を制御することを特徴としている。
すなわち、試料水中に微生物の呼吸を阻害するような有害物質が存在すると、測定溶液中の溶存酸素濃度が上昇して(センサ出力値V2が増加して)、阻害率が上昇する。そして、上記の微生物センサにおいて設定した阻害率の閾値を超えた場合に、試料水中の有害物質検知の警報を出力する。通常、この阻害率が10%以上であれば、検出可能レベルであると判断される。
2 溶存酸素電極
3 リード線
4 フローセル
5 試料流路
6 微生物センサ
7 恒温槽
8 熱交換器
9 制御部
10 エアポンプ
11 流量可変ポンプ
12 定流量ポンプ
13a、13b、13c、13d 電磁弁
14 測定部
Claims (3)
- 微生物を固定化した膜と、溶存酸素電極とから構成される微生物センサを用い、前記微生物の基質を含む緩衝溶液と試料溶液とを混合した測定溶液を前記膜に接触させるように連続的に流し、前記溶存酸素電極からの出力の変化によって、前記試料溶液中の有害物質を検知する有害物質のモニタリング方法において、
以下の(I)式で定義される酸素消費率を所定の範囲内に維持するように、前記測定溶液中の前記基質の濃度を制御することを特徴とする有害物質のモニタリング方法。
酸素消費率(%)={(V1−V2)/V1}×100 …(I)
(上記(I)式において、V1は、有害物質及び基質を含まない緩衝溶液と純水とを流した際の微生物センサの電気的出力、V2は測定溶液を流した際の微生物センサの電気的出力を表す。) - 前記酸素消費率が50〜90%となるように、前記測定溶液中の前記基質の濃度を制御する請求項1に記載の有害物質のモニタリング方法。
- 前記モニタリング中に、前記有害物質を含まない前記測定溶液を前記膜に接触させるように連続的に流す校正を行い、この校正時に前記基質の濃度の制御を行う請求項1又は2に記載の有害物質のモニタリング方法。
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JP4355560B2 true JP4355560B2 (ja) | 2009-11-04 |
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Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
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JP4521319B2 (ja) * | 2005-06-17 | 2010-08-11 | 株式会社東芝 | バイオセンサ型異常水質監視装置 |
JP5129463B2 (ja) * | 2006-06-29 | 2013-01-30 | メタウォーター株式会社 | 水質異常検出法 |
JP2008032691A (ja) * | 2006-06-29 | 2008-02-14 | Fuji Electric Systems Co Ltd | 水質監視システムおよび水質監視方法 |
JP4866304B2 (ja) * | 2007-06-19 | 2012-02-01 | メタウォーター株式会社 | 硝化細菌バイオセンサ応用水質計測器 |
CN101498681B (zh) * | 2009-03-13 | 2012-05-09 | 吴守清 | 测量微量溶解氧的电极 |
JP5347711B2 (ja) * | 2009-05-21 | 2013-11-20 | 新日鐵住金株式会社 | 水中の有害物質の検知方法 |
CN114965617B (zh) * | 2022-06-06 | 2023-11-07 | 北京航空航天大学 | 一种识别电子受体导致的电化学活性微生物检测毒害污染物假阳性的方法 |
CN115639333A (zh) * | 2022-09-30 | 2023-01-24 | 五邑大学 | 一种利用硝化生物反应检测氨氮含量的方法 |
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