JP4272137B2 - 細胞破砕装置及び破砕法 - Google Patents
細胞破砕装置及び破砕法 Download PDFInfo
- Publication number
- JP4272137B2 JP4272137B2 JP2004289554A JP2004289554A JP4272137B2 JP 4272137 B2 JP4272137 B2 JP 4272137B2 JP 2004289554 A JP2004289554 A JP 2004289554A JP 2004289554 A JP2004289554 A JP 2004289554A JP 4272137 B2 JP4272137 B2 JP 4272137B2
- Authority
- JP
- Japan
- Prior art keywords
- cell
- pipette
- cells
- droplet
- tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Description
Claims (5)
- 所定の細胞を含む液滴を表面上に保持するための基板を載置するためのステージと、
細胞を含む溶液を保持でき、所定の大きさの細胞ないし細胞塊が通過できる程度の先端開口径を有するピペットと、
前記ピペットの先端を監視するための手段と、
シリンジポンプおよびその駆動装置を含む、前記ピペットの先端部に細胞を含む溶液を該ピペットの内部から押し出して液滴を形成する手段と、
前記ピペット先端から前記基板上に滴下した液滴に細胞に吸収帯域のある集束光を照射する光学系と
を備える細胞破砕装置。 - 前記基板が、前記細胞を含む液滴を保持するための該液滴のサイズより狭い範囲の親水性領域を有し、該親水性領域の周囲が撥水性である請求項1記載の細胞破壊装置。
- 複数の細胞を含む溶液を保持でき、所定の大きさの細胞ないし細胞塊が通過できる程度の先端開口径を持つピペットと、
前記ピペット先端の細胞を観察する手段と、
シリンジポンプおよびその駆動装置を含む、前記ピペット先端部に細胞を含む溶液をピペット内部から押し出して液滴を形成する手段と、
前記液滴内に所定の細胞が包含され、所定の大きさの液滴となったことを判断する手段と、
前記ピペット先端部に形成された前記液滴を基板上の所定の位置に滴下する手段と、
前記液滴に細胞に吸収帯域のある集束光を照射する光学系と
を備える細胞破砕装置。 - 前記ピペットの内部から押し出された前記液滴が1個の細胞を含む、請求項1〜3のいずれかに記載の装置。
- 請求項1〜4のいずれかに記載の装置を用いて、周囲が撥水性で細胞のサイズより狭い範囲が親水性領域からなる基板表面の該親水性領域に細胞を含む液滴を保持し、液滴中の細胞が光吸収する波長帯域の集束光を照射する細胞破砕法。
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004289554A JP4272137B2 (ja) | 2004-10-01 | 2004-10-01 | 細胞破砕装置及び破砕法 |
EP07013611A EP1901067A3 (en) | 2004-08-03 | 2005-08-01 | Cellomics system |
EP05016714A EP1626278A3 (en) | 2004-08-03 | 2005-08-01 | Cellomics system |
US11/195,662 US7569354B2 (en) | 2004-08-03 | 2005-08-03 | Cellomics system |
US12/143,156 US20090042200A1 (en) | 2004-08-03 | 2008-06-20 | Cellomics system |
US12/143,181 US20090042739A1 (en) | 2004-08-03 | 2008-06-20 | Cellomics system |
US12/471,947 US20100016568A1 (en) | 2004-08-03 | 2009-05-26 | Cellomics system |
US12/472,010 US20100016569A1 (en) | 2004-08-03 | 2009-05-26 | Cellomics system |
US12/471,853 US20100021933A1 (en) | 2004-08-03 | 2009-05-26 | Cellomics systems |
US12/471,993 US20100018862A1 (en) | 2004-08-03 | 2009-05-26 | Cellomics system |
US12/472,037 US20090325215A1 (en) | 2004-08-03 | 2009-05-26 | Cellomics system |
US13/755,079 US20130252848A1 (en) | 2004-08-03 | 2013-01-31 | Cellomics system |
US14/640,471 US20150231635A1 (en) | 2004-08-03 | 2015-03-06 | Cellomics system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004289554A JP4272137B2 (ja) | 2004-10-01 | 2004-10-01 | 細胞破砕装置及び破砕法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2006101718A JP2006101718A (ja) | 2006-04-20 |
JP4272137B2 true JP4272137B2 (ja) | 2009-06-03 |
Family
ID=36372130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004289554A Expired - Fee Related JP4272137B2 (ja) | 2004-08-03 | 2004-10-01 | 細胞破砕装置及び破砕法 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP4272137B2 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7039261B2 (ja) * | 2017-11-17 | 2022-03-22 | 株式会社日立製作所 | 組織切片の特定領域からの生体分子採取方法および装置 |
-
2004
- 2004-10-01 JP JP2004289554A patent/JP4272137B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2006101718A (ja) | 2006-04-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9279749B2 (en) | Laser microdissection method and apparatus | |
EP1775589A1 (en) | Dna chip manufacturing method, manufacturing system, hybridization detection method, detection system, substrate treatment device, and substrate treatment method | |
JP6757730B2 (ja) | マスサイトメトリーによる分析用の構造化生体試料 | |
JP2005534321A (ja) | ホログラフィ式光学トラップで物質を加工、区分け、および統合するための装置および方法 | |
JP2000504824A (ja) | レーザー照射を用いる平坦に配置される生物学的対象への非接触顕微注射、分別及び採取方法並びに装置 | |
WO2013126902A1 (en) | Method and apparatus for light based recovery of sequence verified dna | |
JP2020174598A (ja) | 粒子操作方法、粒子捕捉用チップ、粒子操作システム、及び粒子捕捉用チャンバ | |
JP4272137B2 (ja) | 細胞破砕装置及び破砕法 | |
JP4344474B2 (ja) | レーザ捕捉顕微分析のための機械的処理システム | |
JP2012217397A (ja) | 細胞採取システム | |
US20060023201A1 (en) | Laser microdissection on inverted polymer films | |
JP4216018B2 (ja) | 核酸回収チップと核酸回収装置 | |
JP3820227B2 (ja) | 細胞単離方法 | |
KR102457508B1 (ko) | 기판으로부터 시료를 선택적으로 분리하는 장치 | |
JP7039261B2 (ja) | 組織切片の特定領域からの生体分子採取方法および装置 | |
JP2009207392A (ja) | 増幅核酸の解析方法及び解析装置 | |
US20190056294A1 (en) | Methods for selectively separating samples from substrate | |
JP4194988B2 (ja) | 電気泳動分離物の回収装置と回収方法 | |
CN114729893A (zh) | 用于对样本进行成像和消融的系统和方法 | |
JP4467422B2 (ja) | 生体分子検出装置および生体分子検出法 | |
JP2018091634A (ja) | 試料の処理方法、および試料処理用キット |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060116 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20070125 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20070125 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20070319 |
|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20070703 |
|
A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20080213 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20081104 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20081224 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20090203 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20090226 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120306 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130306 Year of fee payment: 4 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20140306 Year of fee payment: 5 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |